Download - Swapnil Poster Presentation 23 Final
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QUALITY BY DESIGN CONCEPT TO
ADVANCEMENT IN TECHNIQUES
IN BRAIN CANNULATION SURGERYIN RATS.
CHAUHAN S. B*. AND ANSARI M. A.,
UPADHYAY M. & TAKSANDE B.G.
Smt. Kishoritai Bhoyar College OfPharmacy, Kamptee.
Dist-Nagpur Pin:-441002
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*Brain canulation surgery in rats is necessary for injection of
anatomical tracers, implantation of electrodes, microdialysis
probes. etc
Steps in rodents stereotaxic surgery say for
example in ICV cannula implantation
1) Anesthesia
2) Placement
3) Intracranial surgery
4) Location of bregma
5) Mesurement of co-ordinates
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6) Drill a burr-hole through skull over co-ordinate/s
7) Implantation of guide cannula
8) Cementation of guide cannula.
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*A cannula or canulais a tube that can be inserted into the
body, often for the delivery or removal of fluid or for the
gathering of data.
*Brain cannulation surgery stereotactic aparatus are used
*Stereotaxis: Use of a coordinate system to provide accuratenavigation to a point or region in space.
*Stereotaxic (or stereotactic) is used to target specific brain
regions by precisely placing a subject's head into a rigid frame(the Horsley-Clarke frame) and positioning an instrument at
predetermined distances from reference landmarks.
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Single Point Stereotactic Apparatus.
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*In the rodent brain the conventional landmarks are
bregma for rostral and interaural for caudal targets.
*Stereotaxic methods are also used for radiosurgery and
other therapeutic approaches such as transplantationand gene therapy.
*Traditional stereotaxic atlases derived from such
positioning protocols use two-dimensional drawings ofserial sections to describe the three-dimensional (3D)
location of brain structures.
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Stereotactic Atlas
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DISADVANTAGES
*The main disadvantage of above stereotaxic model is
having only one pointer hence if two or more surgery isto be done in different cerebral hemispheres it would be
very difficult.
*One pointer can locate only one desired site at a time
and shifting of the pointer to another site would be time
consuming.
*Shifting of the pointer to different location may provide
time to animal to recover from anesthesia.
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*Hence another kind of stereotaxic instrument can be
used in such instant.
Double Point
Stereotactic
Apparatus.
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ADVANTAGES:
1) More than one surgery can be performed at a time
2)It is time saving
DISADVANTAGES:
*The common disadvantages of the above two
stereotaxic systems are human operational errorbecause of confusion in acquiring co-ordinates.
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*Another advancement made in such instruments are
the introduction of computer software which
eliminates human operational error such as settingwrong stereotactic co-ordinates.
Computer Aided
Stereotactic Aparatus.
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ADVANTAGES:
*A correct placement of the subject in the stereotacticframe is achieved, as all the calculations rely on an
accurate positioning of the landmarks relative to the
coordinate system using computer aided stereotacticsystem.
*Another advantages are:
1) Greater accuracy
2) Greater precision
3) Greater Robustness
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Reference :-
*DAVID KOPF INSTRUMENTPrecision Designs for Research.
*Stereotaxic Surgery in the Rodent CNS: Basic Protocols
and ApplicationsHarvard Center for Neurodegeneration and Repair
(HCNR)--Brain Repair and Regeneration Core.