Download - Stam Cell Niche
Department of Hematology School of Medical
Sciences Tarbiat Modares
University
Najmaldin saki
Stem Cell Niche
: مسعود دکتر راهنما استادسلیمانی
الدین : نجم کننده ارائهساکی
HSCs
MSCs
CLP
CMP
GMP
MKEP
Cell fates available to a stem cell
Quiesce Differentiate
Self-renew Apoptose
Self-renewal or differentiation
Hematopoietic Stem Cell(HSC)
Hematopoietic Stem Cell(HSC)
Ratio in BM : 1̸$1̸0000 to 1̸$1̸00000
Characteristic:Sca-1̸Thy.1̸ LOW
Lin-(HLA-DR, CD71̸, CD45RA,CD38)
rTpo(C-MPL)CD1̸33+CD34+CD1̸1̸7+
Mobilizing:G-CSF or rh G-CSFM-CSFSCFIL3TpoMIP-1̸α
Stemness:Jagged-1̸N-Cadherin
Endothelial Cell
Clathrin-Coated pits
ED Cells:VWFCol IVLamVCAM1Selectin ECD34+CD14+CD45-SDF-1IL6
Ulex europeausAb CD34
CD22
Adventitial reticular cells
Characteristic:Alp +CD1̸0+CD1̸3+HLA IAlpha smoth-muscle actinVementinLamininFibronectinColl I,III,IVCD34-
receptor for:Nerve growth factor
Adipocytes
BM reticular cells ………..Adipocyte Starvation Saturated fatty acid
Express:LeptinOsteocalsinProlactin receptorVEGF
Maturin in vitro inhibited by:IL1̸ & IL1̸1̸
Stem cell erythroiedLeptin vs EPO
Fibroblasts
CD1̸4-
CD34-
CD45-
VCAM-1̸+
α-smooth muscle protien(ASP)α-smooth muscle actin(ASA)
Receptor for :bFGFPDGFEGF
TGFβ
Secret:GM-CSFM-CSFCSFIL6LIF
Secret:CollFibrinectinLamininProteogglycopro
Osteoblasts
Markers:CD34-STRO-1+RANKLSecret:TGFβB-FGFBMP-2M-CSFG-CSFGM-CSFIL1IL6ALPOstepontin(OPN)OsteocalcinCollfibronectinOPG
PTHWnt/βcatenin
osteoblastN-cad+
N-cad-Spindle shaped N-cadherin positive osteoblast
markers of primitive human marrow stromal cells and their more differentiated progeny
osteoclasts
CD34+STRO-1-TRAP+RANKCD51+CD54+CD68+
Secreat:MMPCatepsin K
M-CSF has a critical rol in Osteoclastojenesis
Osteopetrosis&
Osteoporosis
Vascular niche
Endosteal niche
M-CSF & RANKL & OPGIL7
Ost
HSC
HSC
مغز در رگ اندوتلیال نیچ) فعال) سلول استخوان
استئوبالست نیچمغز در
سلول) استخوانخاموش(
تمایزسلول بنیادی خونساز
ه رد یید
وئریت
ار
ه رد ست
الوب
ریکا
گام
یه
رد یید
وئیل
م
ه رد یید
وئفلن
O2
O2
غزم
یها
رگره
وادی
لیاتلدو
انی
هاول
سل
نوا
خست
ا
ObOs
CD1̸38
OPG
RANKL
RANK
MM
RANKL/OPG ratio
Enucleation of late-stage erythroblasts leads to the formation of two cells – a reticulocyte and a pyrenocyte
Pathways Involved in Stem CellSelf Renewal and Cancer
• Notch
• Hedgehog
• Bmi-1
• Wnt
Wnt
Extracellular space
CYTOPLASM
NUCLEUS
B-cateninB-catenin
B-catenin
GSK3
glycogen synthase kinase 3 APC
adenomatous polyposis coli
Axin
P
B-catenin
NUCLEUS
Extracellular space
CYTOPLASM
LRP
LRP
low-density lipoprotein receptor–related protein 5 and 6 (LRP5/6)
WntWnt inhibitory factor 1
frizzled-related protein
LRP
Kremen
SOSTDKK
Sclerostin(SOST gene product)
Dickkopf (Dkk) families
Interaction of the Dkk/LRP complex with kremen internalizesthe complex for degradation, thus diminishing the number
of Wnt coreceptors available for signaling
LRP
Wnt
GSK3
APC
Axin
Frat-1
Dsh
B-cateninB-catenin
B-cateninB-catenin
B-catenin
B-catenin
silencing mediator of retinoid and thyroid receptors and nuclear receptor corepressor
T cell factor/lymphoid enhancer binding factor
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