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QIAgility ─ Pure Precision
Andrea TesorieroApplication Specialist
QIAGEN 2009
Sample & Assay Technologies
First we automated manual spin column
preps…
You Have More Important Things To Do….
Now we are able to automate all manual
pipetting in the lab…
20092007
Now QIAGEN takes the next step and enables another convenience shiftNow QIAGEN takes the next step and enables another convenience shift
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From Manual to Automation
Higher Precision and more time every day for…
Preparing & designing new experiments
Data analysis
Literature search
Writing scientific articles
And much more…
Eliminating tedious pipetting in the lab!
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PrecisionResults achieved exceed the best hand pipetting
VersatilitySupports all different cycler formats in the market
Ease-of-useWhat-you-see-is-what-you-get software interface
Speed
Streamlined workflow by
Developed For PCR SetupQIAgility Main Advantages
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QIAgility performs all “tricks” an experienced technician uses
while pipetting
Dynamic liquid level sensing limiting tip immersion
Tracking the meniscus in each tube to minimize tip
retention
Retracting the tip while dispensing
Minimizing Tip/Liquid contact
Slowing down pipetting speed for viscous liquids
Avoiding formation of bubbles in tip
Selecting automatically which tip (50µl or 200µl) to select
maximizing precision
High Precision Meets Pipetting SkillsPrecision Aspects
Precision
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No matter what real-time instrument you use, QIAgility is
compatible
The system is pre-configured to work with all PCR tube
types and all plate formats, including varieties of 96-
well, 384-well and Rotor-Disc
Beside any PCR setup the system can perform
Set-up of quantification standards (dilution series)
Normalization of sample concentration
Sample pooling
Transfer of samples from one tube format to
another
Automatic set up for multiple variables
Post PCR setup
The Hallmark Of QIAgility Is Its VersatilityVersatility Aspects
Versatility
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VersatilityQIAgility supports all real-time PCR formats
LightCycler™ CapillariesRotor-Gene™ 0.2 mL, 0.1 mL and
Gene-Disc™ formats
SmartCycler™ Tubes
Roche COBAS™
96-well and 384-well(Incl. all ABI, Bio-Rad, Stratagene, Eppendorf etc. instruments)
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Versatility96 and 384-well support
Compatible with plates, tubes, tube racks, cooling blocks etc
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VersatilityUnusual formats are also supported
Including: MALDI plates, ELISA plates, reservoir plates, etc, etc
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The Intuitive Software Is Easy To UseSoftware Features
The software mirrors the actual deck setup
Intuitive layout—simple to learn
Calculation of reagent needs according to the
number of reactions and replicates specified
Tip re-use options
Import/Export of sample names
Pre- and Post-run reports including worktable
setup guide
Virtual mode aids new protocol testing
Wizards for speedy PCR setup and optimization
Ease of Use
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You Have More Important Things To Do….QIAgility Means Time Savings
“If you worked once with the QIAgility, you don’t
remember how you have managed without…”
Staff can be attending to other duties while the
QIAgility is running
Simple Transfer Actions 1:1
– 96 samples
• 17 minutes
• 6 minutes with tip reuse
Transfer Actions with pre-MM
– 96 samples PCR set-up
• ~25 minutes
Transfer Actions with pre-MM
– 192 samples PCR set-up
• ~46 minutes
Speed
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Unrivaled Pipetting PrecisionRotorGene Probe RT PCR Kit
Data presented in brochure table 1
Precision
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Exceptional Reproducibility and Accurate ResultsRotorGene Probe PCR – Template dilutions
Setup Details
Target: IL1 R2
Sample: Hs gDNA
10 twofold dilutions
(30ng – 60pg)
5 replicates
Rotorgene 6000
Data presented in brochure figure 1
Precision
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Automated Workflow for Accurate ResultsRotorGene Probe RT PCR Kit
Setup Details
Target: IL1 2RB1
Sample: Leuko RNA
Standard row in triplicates
5 tenfold dilutions (10 pg – 100 ng)
Rotorgene 6000
Data presented in brochure fig 3
Precision
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Worktable setup example
Worktable Positions
1. Master Mix Block
2. Reagent Block
3. Tips
4. Tips
5. Tips or Rotor-Gene Q Loading Block
6. Tips
7. Sample Plate or Rotor-Gene Q Loading
Block
8. Reaction Plate
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3 4
5 6
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3 4
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Guides you through creating a
run file with master mix, standard,
Sample and final reaction!
Life Can Be So Simple….QIAgility PCR Setup Wizard
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Just a few simple steps
Define Plate/Tube Format Sample Bank (number of
samples) Reagents (primer, buffer, taq,
etc.) Master Mix (pre-mix, setup) Standards Reactions
Add Controls Add Standard Add Samples
GO!
Set Up Your Own RunSoftware Explanation
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N View the software