Download - Rb cs count
![Page 1: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/1.jpg)
Red cell countRed cell count
![Page 2: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/2.jpg)
MethodsMethodsA. Manual diluting method.
Principle:Using a diluting fluid that destruct all blood cells
leaving only RBCs intact.
With the aid of a hemocytometer, counts the number of cells expressed in millions per micro liter of blood.
![Page 3: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/3.jpg)
ReagentsReagents
Gower’s solution: Sod. Sulphate, Glacial acetic acid and D.W
Hayem’s solution: mercuric chloride, NaCl, sod. Sulphate and
D.W. Physiological saline (0.85%)
![Page 4: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/4.jpg)
EquipmentsEquipments::
HemocytometerCover slipPipettes
![Page 5: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/5.jpg)
ProceduresProcedures::1. Dilution:
Add 2 ml of hayem’s solution to clean and dry tube.
Add 10 µl of the sample (whole anti-coagulated blood) and mix.
Dilution factor: 1:200
![Page 6: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/6.jpg)
2. Charging
Charge the hemocytometer between the cover slip and the counting chamber.
Leave the cells to settle down.The depth factor is 1\10
3. Counting:
Count the RBCs in 5 medium squares (4 at the 4 corners & 1 in the center) inside the central large square.
Count those cells in L-shape manner
![Page 7: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/7.jpg)
![Page 8: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/8.jpg)
![Page 9: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/9.jpg)
![Page 10: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/10.jpg)
![Page 11: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/11.jpg)
![Page 12: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/12.jpg)
![Page 13: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/13.jpg)
16 Tertiary squares
![Page 14: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/14.jpg)
Reading (counting)Reading (counting)????? Cell /square.
![Page 15: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/15.jpg)
CalculationCalculation::Erythrocytes / µl of blood= cells counted per
square х 10 (depth) х 5 х200(dilution)
ORErythrocytes / µl of blood= cells counted per
square х 10 (depth)х 25х200 (dilution)
= …………………. Cells / µl or cells / mm3
![Page 16: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/16.jpg)
B. Other automatic B. Other automatic methodsmethods : :
Electronic cell counterFlow cell cytometers : depends
on laser lights.
![Page 17: Rb cs count](https://reader033.vdocuments.us/reader033/viewer/2022061604/5560e1c3d8b42a3d768b4aaf/html5/thumbnails/17.jpg)