Download - Proteomics: 2D Gel
Proteomics: 2D Gel
Proteome analysis 장치 (2D)
Root Proteome Nodule Proteome
Table I. Proteins identified in soybean root and nodule through MALDI-TOF/MS & LC-MS/MS
Spot No.
Calc. pI/ Mwa
Z-scoreb / Mascot scorec
Identifyd
Species (NCBI accession No.)
Theor. pI/MW
SCe (%)
StatusAnaly-zerf
Ⅰ. Primary metabolism
sr038g 6.19/41 1.65/ -Cytosolic malate dehydrogenase
Cicer arietinum(CAC10208)
5.9/35.82 24 . m
sr150 5.98/42 0.99/ -Malate dehydrogenase, cytoplasmic
Beta vulgaris(Q9SML8)
5.9/35.82 20 5d-up m
sr151 5.98/42 1.1/ -Malate dehydrogenase, cytoplasmic
Beta vulgaris(Q9SML8)
5.9/35.82 20 5d-up m
sr160 6.42/41 0.85/ -Malate dehydrogenase, Mdh-2, mitochondrial
Glycine max(T06326)
5.6/27.54 15 . m
sr161 6.42/41 1.6/ -Malate dehydrogenase, Mdh-2, mitochondrial
Glycine max(T06326)
5.6/27.54 21 . m
sn125h 6.41/38 -/ 741 Malate dehydrogenase
Bradyrhizobium japonicum USDA 110(NP_767096)
5.88/34.1 44.7210d-new, 13d-up
t
ssn002 6.00/43 -/ 62 Malate dehydrogenaseGlycine max(AAD56659)
8.23/36.1 8.11 10d-new t
sr101 5.40/40 0.23/ -pfkB-type carbohydrate kinase family protein
Arabidopsis thaliana(NP_180697)
5.3/35.43 18 . m
sr112 5.30/40 0.89/ -pfkB-type carbohydrate kinase family protein
Arabidopsis thaliana(NP_180697)
5.3/35.43 24 . m
단백질의 질량 스펙트럼
단백질
효소
질량분석기MS spectrum
Peptide sequenc
es
Proteindatabase
Protein id
A
B
A B
Proteomics: MALDI-Mass
Mass spectrum vs. database
Mass spectrum
database
Protein A
Protein B
Protein C
탄뎀 질량 스펙트럼(MS/MS spectrum)
단백질
효소
1st MS 2nd MS
MS spectrum
energy
Tandem MS
spectrum
Peptide sequenc
e
Proteindatabase
Protein id
탄뎀 질량 스펙트럼Peptide fragmentation
H2
NCOOH
R1
C
H
O
N
H
C
R2
C
H
O
N
H
C
R3
C
H
O
N
H
C
R4
C
H
x3 y3 z3 x2 y2 z2 x1 y1 z1
a1 b1 c1 a2 b2 c2 a3 b3 c3
2D data 분석