Solis BioDyneProduct Catalogue 2017Ambient Temperature Stable PCRand qPCR Reagents
www.sbd.ee
Dear Partners,Thank you for trusting Solis BioDyne with your research.
The cooperation we have built with you over the years has become more personalized and synergetic.
This ever closer relationship helps us understand your needs better and provide the solutions that precisely meet your requirements. In this 2017 edition of our catalogue you will see our most popular items, but keep in mind we love to discuss about your projects and help you tackle your PCR challenges.
Best regards,
Kadri Artma Managing Director
About UsSolis BioDyne manufactures high quality molecular biology reagents since 1995. The company is based in Tartu, Estonia, an international academic city with a growing biotechnology sector.
Our young and professional team is dedicated to provide:
• high quality products• cost effective solutions• quick and professional service
Our product line includes DNA polymerases, master mixes for PCR/qPCR and other reagents – all stable at ambient temperature. Each product batch goes through very strict quality control system based on ISO 9001:2015 standard.
Greetings from the Solis BioDyne Team
PRODUCT CATALOGUE 2017 1
Stability TAG Technology 2
qPCR Mix Specifications & Mix Compatibility Table 4
Dye-based qPCR Mixes 6
Probe-based qPCR Mixes 9
Product selection Guide:
Standard PCR Enzymes, Master Mixes and Other Enzymes 11
Hot-start PCR Enzyme and Mixes 12
Standard PCR Enzyme and Mixes 16
Other Enzymes:
TERMIPol DNA Polymerase; M-MLV Reverse Transcriptase 18
Other Reagents:
dNTP Mix and Set, DNA Ladders, DNA Loading Dye Buffers etc. 20
Ordering 23
Distributors 25
Product List 26
Table of Contents
Riia 185a, 51014 Tartu, EstoniaTel: +372 7409 960Fax: +372 7402 079E-mail: [email protected]: solis.biodyneWeb: www.sbd.ee
Solis BioDyneVAT No: EE100587614Reg. No: 10242922
Bank details:Swedbank ASIBAN code: EE692200221005142234SWIFT/BIC.: HABAEE2XBank address: Liivalaia 8, 15040 Tallinn, Estonia
2
Taq DNA Polymerase Stability TAG
Ice-free reaction set-upOur temperature stable enzymes allow you to change the way you work with enzymes that are usually extremely sensitive to tem-perature changes.
• more space on your work station and in your freezer• convenient working conditions• less energy used for making ice or keeping bench-top coolers
cold
Polypeptide Stabilization Technology: Stability TAG
REACTION SET-UP ON ICEREACTION SET-UP AT ROOM TEMPERATURE
3D MODEL OF FIREPOL® DNA POLYMERASE INCLUDING STABILITY TAG
Barleygenomic DNA
HOT FIREPol® DNA Polymerase
Mousegenomic DNA
Barleygenomic DNA
Mousegenomic DNA
FIREPol® DNA Polymerase
-20°C1 2 2 weeks at +35°C 3 4 weeks at +35°C
1 2 3 1 2 3 1 2 3 1 2 3
“ Solis BioDyne products are excellent products and meet the
following criteria:
• Quality: they give good results and a sharp signal without any
background noise.
• Cost: They are inexpensive, the order process is fast and the fact
they are storable/shipped at room temperature reduces the cost of
transportation.
• Convenience: no need to order separately the PCR products as
everything is inside the Mix which reduces pipetting errors. It is not
necessary to work in ice which helps to save energy and avoid the
deterioration of product due to power cuts or forgetting the
product at room temperature. ”ABDUL AZIZ WANE Biology and Sanitary Engineer Institut Pasteur Dakar, SENEGAL
Our technologyWe have genetically modified our enzymes to give them improved long term stability and enhanced stability at ambient temperatures. The addition of the Stability TAG (EU Patent EP2501716 and US Patent No 9,321,999) ensures that our enzymes are fully active even after keeping them at room temperature for a month!
Worry-free storageAccidents can happen to anyone. Our reagents will still remain fully active after a power outage has caused the loss of everything in your freezer, after someone has forgotten the reagents on the table overnight or if customs delays the delivery.
Our thermostable DNA polymerases HOT FIREPol® and FIREPol® show no activity loss after incubation at +35oC for up to 4 weeks.
PRODUCT CATALOGUE 2017 3
Ca savings
Ca savings
Ca savings
No need to pay for shipping the ice
DRY ICEIN REGULAR
INSULATED BOX
ACTUALPRODUCT SIZE
Ice-free shippingOur Stability TAG technology means we can ship your order without dry ice or using large insulation boxes. Which is better for the environment and your budget:
• less material used for packaging
• lower package weight
• less fuel used for transportation
• lower shipping charges for you
Find out how much you can save when you don't have to pay for shipping the dry ice
4
qPCR Mixes: Specifications
HIGHLY COMPETITIVE
Four five-fold dilutions of 98 bp fragment of GAPDH gene were amplified from human genomic DNA using 5x HOT FIREPol® EvaGreen® qPCR Supermix (red) and qPCR mixes from companies A, B and C (dark blue, light blue and green). Reactions were per-formed on Applied Biosystems ViiATM 7 Real-Time PCR System following cycling pro-tocol recommended by the supplier.
1000
0010
000
∆Rn
Cycle20 22 24 26 28 30 32 34 36 38 40
Solis BioDyne Supermix Comp. A Comp. B Comp. C
Higher precision and wider scaleAll PCR and qPCR mixes from Solis BioDyne are produced as 5x mixes that occupy only one fifth of the reaction final volume leaving 2.5 times more room for the template compared with 2x mixes. This is highly advantageous when making wide-range serial dilutions or in case of gene expression studies where the concentration of the target gene is very low. Using the 5x mix enables the researcher to add more cDNA to the reaction which can result in better precision and lower Cycle threshold (Ct) values.
Light protective packagingDNA intercalating dye EvaGreen® and reference dye ROX necessary in many qPCR mixes are sensitive to light and therefore require protective shielding. Solis BioDyne packs all these qPCR mixes in special dark tubes to prevent harm from potential light exposure during transportation and reaction set-up.
Get 2,5x more reactions done with same volume of qPCR Mix
COMPARISON OF OUR 5X QPCR MIX TO STANDARD 2X QPCR MIX
Vial sizeReactions (20 µl
final volume)
2x Standard qPCR Mix 1 ml 100 rxn
5x Solis BioDyne qPCR Mix
1 ml 250 rxn
Quality ControlTo ensure highest quality and reproducible results in your qPCR assay, every product lot goes through strict quality control proce-dure including the following tests below:
• Amplification efficiency ≥ 105 fold• Functional quality control via qPCR on different templates• No ds Endodeoxyribonuclease activity• No ss Exodeoxyribonuclease activity• No self-priming activity
EvaGreen® dyeAll Solis BioDyne dye-based qPCR Mixes contain next- generation DNA-binding dye with features ideal for use in quantitative qPCR and HRM. EvaGreen® dye is non-toxic, non-mutagenic, and not hazardous to aquatic life.
• Highly sensitive – produces the most robust PCR signal when used at the recommended concentration
• Low PCR inhibition – exhibits much less PCR inhibi-tion than other detection dyes via a smart “release on demand” DNA-binding technology
• Extremely stable – simply indestructible under most biochemical conditions. Can be stored at ambient tem-perature and be subject to repeated freeze-thaw cycles
• Spectrally similar to other popular dyes – compatible with all major brand real-time thermal cyclers
• Environmentally safe – non-mutagenic, non-cytotoxic; safe to aquatic life for easy handling and disposal down the drain.
PRODUCT CATALOGUE 2017 5
qPCR Mix Compatibility Table:Dye and Probe-based qPCR Mixes
Dye-based approachProbe-based
approach
5x H
OT
FIRE
Pol®
Eva
Gre
en®
qPCR
Sup
erm
ix
5x H
OT
FIRE
Pol®
Eva
Gre
en®
qPCR
Mix
Plu
s (R
OX)
5x H
OT
FIRE
Pol®
Eva
Gre
en®
qPCR
Mix
Plu
s (n
o RO
X)
5x H
OT
FIRE
Pol®
Eva
Gre
en®
qPCR
Mix
Plu
s (C
apill
ary)
5x H
OT
FIRE
Pol®
Eva
Gre
en®
HRM
Mix
(RO
X)
5x H
OT
FIRE
Pol®
Eva
Gre
en®
HRM
Mix
(no
RO
X)qPCR Platforms
5x H
OT
FIRE
Pol®
Pro
be q
PCR
Mix
Plu
s (R
OX)
5x H
OT
FIRE
Pol®
Pro
be q
PCR
Mix
Plu
s (n
o RO
X)
5x H
OT
FIRE
Pol®
Pro
be q
PCR
Mix
Plu
s (C
apill
ary)
5x H
OT
FIRE
Pol®
Pro
be
Uni
vers
al q
PCR
Mix
√ √ √
Applied Biosystems: 5700, 7000, 7300, 7700, 7900 HT, 7500, StepOne™, StepOnePlus™, ViiA™7, QuantStudio™ 3, 5, 6 Flex, 7 Flex, 12K Flex Real-Time PCR System
√ √
√ √Agilent/Stratagene: Mx3000P™, Mx3005P™, Mx4000PM
√ √
√ √ √BioRad: CFX96™, CFX384™, iQ™5, MyiQ™, Chromo4™, Opticon®2; MiniOpticon®
√ √
√ √ Cepheid®: SmartCycler® √ √
√ √ Eppendorf: Mastercycler® ep Realplex √ √
√ √ √Qiagen: Rotor-gene® 3000, Rotor-gene® 6000, Rotor-gene® Q
√ √
√ √ √ Thermo Scientific: PikoReal™ √ √
√ √ √ Illumina: The Eco™ √ √
√ √ √Roche Applied Science: LightCycler® 480, LightCycler® Nano, LightCycler® 96
√ √
√ Roche Applied Science: LightCycler® 1.x, 2.0
√
FIREPol is a trademark of Solis BioDyne. Applied Biosystems and StepOne are trademarks of Applied Biosystems LLC. QuantStudio and ViiA are trademarks of Life Technologies Corporation. Stratagene, Mx3000P, Mx3005P and Mx4000 are trademarks of Agilent Technologies inc. Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a trademark of Qiagen Group. Bio-Rad, CFX96, CFX384, iQ,MyiQ, Opticon 2, Chromo4, MiniOpticon are the trademarks of Bio-Rad Laboratories. Smartcycler and Cepheid are the trademarks of Cepheid Corporation. LightCycler and TaqMan are trademarks of Roche Molecular Systems Inc. The Eco is a trade-mark of Illumina Inc. PikoReal is a trademark of Thermo Fisher Scientific Inc. EvaGreen is a trademark of Biotium Inc. 3D model of FIREPol DNA Polymerase by Aare Abroi (Tartu University) and Sebastien Langui (Solis BioDyne), picture on the cover by Remo Savisaar.
6
5x HOT FIREPol® EvaGreen® qPCR Supermix
Send your sample request to [email protected]
PRODUCT CAT. NO. 20 UL REACTIONS SIZE/ml
5x HOT FIREPol® EvaGreen® qPCR Supermix
08-36-0000S (free sample) 08-36-00001 08-36-00008 08-36-00020
5025020005000
0.21820
Researchers already trust Supermix
Reference:
“ I use Solis Supermix to analyse ChIP DNA and find it extremely reliable. Even with higher cycle numbers the replicates of the reaction give very stable results. We have also tested a qPCR mixture from another supplier and found that Supermix gives higher quality PCR products (single intensive band vs multiple bands of varying intensity). After that discovery we instantly switched to Solis mixture for all our qPCR reactions. ”SIGNE VÄRV PhD, post-doctoral researcher University of Bergen, Norway
Amplification plots of tenfold dilution series for the human GAPDH gene performed on Applied Biosystems™ ViiA™7 (upper graph) and Roche LightCycler® 480 (lower graph). The amount of DNA per reaction ranges from 0,01 to 10 ng. The results show high linear range and high efficiency across a wide range of DNA concentrations on different qPCR platforms.
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
Selected publications:
• Urgard, E., et al. J. Control. Release (2016).
• Bednarek, K., et al. Tumor Biol. (2016).
• André, L., et al. Bioresource technology (2016).
• Inturi, R., et al. Virology (2015).
• Gajardo, T., et al. Immunology (2015).
DescriptionPrecisely optimized real time qPCR master mix based on DNA binding dye technology. This master mix has been developed to give highly specific and sensitive results.
Benefits• High sensitivity with low DNA concentrations• Reduced primer dimer formation• Reaction set-up and shipment without dry ice• One qPCR mix for all cyclers (except capillary)• Contains dUTP to prevent cross-contamination with UNG
treatment
Tip!EvaGreen dye is a
superior analogue to SYBR Green I dye.
You can keep using the same fluorescence
detection settings.
1E01
1E00
∆Rn
0.20.1
0.0118 20 22 24 26 28 30 32 34 36 38 40 42 44
Cycle Number
0,2Efficiency: 97.9 Slope: -3.37 R2:1
31.225
21.225
Fluo
resc
ence
(46
5-51
0)
11.225
1.225
18 20 22 24 26 28 30 32 34 36 38 40 42 44Cycle Number
Efficiency: 101,25Slope: -3.263
TRUSTWORTHY PERFORMANCE
PRODUCT CATALOGUE 2017 7
Researchers already trust EvaGreen® Plus
Reference:
“ We have been using the 5X EvaGreen® Master Mix for a year. It works perfectly well for us! Compared with the products from other companies, its price is significantly lower and the quality of result is equally good or better! ”SUN, LEI PhD, Assistant Professor Cardiovascular & Metabolism Disorders Programme Supplied by TLC Scientific Solutions
10
0.1
0.2
0.0115
1
17 19 21 23 25 27 29 31 33N
orm
. flu
oro.
Cycle Number35
Excellent sensitivity and specificityThe amplification of 98 bp fragment of GAPDH gene exhibits sensitive and efficient reaction curves (upper graph) with highly specific peak in melt curve analysis (lower graph) using HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX). Amplification was performed on human genomic DNA using Rotor-Gene® 6000 qPCR cycler following cycling protocols recommended by the supplier.
66 70 74 78 82 86 90 94
dF /
dT
°C
2.8
2.4
2
1.6
1.2
0.8
0.4
0
EXCELLENT SENSITIVITY AND SPECIFICITY
The amplification of a 98 bp fragment of GAPDH gene exhibits sensitive and efficient reaction curves (upper graph) with highly specific peak in melt curve analysis (lower graph) using HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX). Amplification was performed on human genomic DNA using Rotor-Gene® 6000 qPCR cycler fol-lowing cycling protocols recommended by the supplier.
Send your sample request to [email protected]
PRODUCT CAT. NO. RXN/20µl SIZE/ml
5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)
08-24-0000S (free sample)08-24-0000108-24-0000808-24-00020
5025020005000
0.21820
5x HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX)
08-25-0000S (free sample)08-25-0000108-25-0000808-25-00020
5025020005000
0.21820
5x HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary)
08-26-0000S (free sample)08-26-0000108-26-0000808-26-00020
5025020005000
0.21820
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
Selected publications:
• Krjutškov, K., et al. Scientific Reports (2016).
• Boija, A., Mannervik, M. Proceedings of the National Academy of
Sciences (2016).
• Mirzamohammadi, F., et al. Nature Communications (2016).
• Fliedl L., et al. New Biotechnology (2015).
• Anderson G.W., et al. Stem Cell Research & Therapy (2015).
DescriptionCost-effective real time qPCR master mix based on DNA binding dye technology. Provides highly reliable and reproducible results.
Benefits• High sensitivity and specificity• Excellent efficiency • Reaction set-up and shipment without dry ice• Master mixes for ROX, no ROX and capillary cyclers
5x HOT FIREPol® EvaGreen® qPCR Mix Plus
8
5x HOT FIREPol® EvaGreen® HRM Mix
DescriptionReliable and sensitive real time qPCR master mix for high resolution melt (HRM) analysis. This master mix uses the next-generation DNA binding dye - EvaGreen®.
Benefits• Reaction set-up and shipment without dry ice
• Mixes available for ROX and no ROX cyclers
• Sensitive EvaGreen® dye allows detection of DNA sequence variations
Researchers already trust EvaGreen® HRM Mix
Reference:
“ We appreciate your products because most of our research takes place in Kenya and it can be difficult to ship items that are temperature sensitive. The Hot Firepol® Evagreen® HRM Mix is very effective and the prices are reasonable. ”DR JEREMY HERREN International Centre of Insect Physiology and Ecology, Kenya
SENSITIVE HRM GENOTYPING
High Resolution Melt Analysis was used to genotype a C insertion in BRCA1 gene, a breast cancer suscepti- bility gene, with HOT FIREPol® EvaGreen® HRM Mix (two graphs above). Reactions were performed on Corbett Rotor-Gene® 6000. Green lines represent wildtypes with-out an insertion, red lines represent a C insertion and blue line represents a patient with unknown phenotype.
Send your sample request to [email protected]
PRODUCT CAT. NO. RXN/20µl SIZE/ml
5x HOT FIREPol® EvaGreen®
HRM Mix (ROX)
08-33-0000S (free sample)08-33-0000108-33-0000808-33-00020
5025020005000
0.21820
5x HOT FIREPol® EvaGreen®
HRM Mix (no ROX)
08-31-0000S (free sample)08-31-0000108-31-0000808-31-00020
5025020005000
0.21820
90
70
50
30
10
77 78 79 80 81 8382
Nor
mal
ized
Flu
ores
cenc
e
°C
12
10
8
6
4
2
0
-277 78 79 80 81 82
Nor
mal
ized
min
us 5
382
mut
insC
°C83
No.
37
40414243
Name
Unknown phenotypeWildtype 1Wildtype 2Mutation 1Mutation 2
Genotype
5382 wt
5382 wt5382 wt5382 mut insC5382 mut insC
Confi. %
99,18
97,33100,00100,0097,47
Color
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
Did you know?High resolution melt
analysis can be used for SNP genotyping, discovering mutations, screening for heterozygosity, analysing
DNA methylation.
Selected publications:
• Blouin, A. G., et al. Molecular Ecology
Resources (2016).
• Khori, V., et al. Lasers in Medical
Science (2016).
• Borecka, M., et al. Cancer Genetics (2016).
• Kim S.Y., et al. Journal of Microbiology
(2015).
• Rawluszko A.A., et al. BMC Cancer (2013).
PRODUCT CATALOGUE 2017 9
Send your sample request to [email protected]
PRODUCT CAT. NO. RXN/20µl SIZE/ml
5x HOT FIREPol® Probe Universal qPCR Mix
08-17-0000S (free sample)08-17-0000108-17-0000808-17-00020
5025020005000
0.21820
5x HOT FIREPol® Probe Universal qPCR Mix
QPCR PERFORMANCE IN A DUPLEX REACTION:
Two genes from human gDNA were amplified in duplex reaction using HOT FIREPol® Probe Universal qPCR Mix. Excellent results were obtained from four 10x dilu-tions (starting from 10 ng/µl) from both genes. BAIP3 (blue) with GC-content 70,3% and efficiency 100% and GAPDH (yellow) with GC-content 56,1% and efficiency 98,4%. Reactions were performed on Applied Biosystems ViiATM 7 Real-Time PCR System.
12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44
1E00
0.01
Amplification Plot
∆Rn
CYCLE
HIGHLY COMPETITIVE QPCR MIX:
Four 10x dilutions of 197 bp long fragment of B4G4 gene with GC-content 75,6% were ampified from human gDNA using 5x HOT FIREPol® Probe Universal qPCR Mix (blue) and qPCR Mix from another vendor (green). Reactions were performed on Applied Biosystems ViiATM 7 Real-Time PCR System following cycling protocol recommended by each supplier.
Solis BioDyne Competitor
1.3
1.2
1.1
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
18 20 22 24 26 28 30 32 34 36 38 40 42 44
∆Rn
CYCLE
Researchers already trust Probe Universal
Reference:
“ We were using Firepol® Master Mix for our conventional PCR already and now we are developing Real-Time PCR and we were testing var-ious mixes. From the 3 different ones we tested, best results were with Solis Probe Universal qPCR Mix. ”ELENA IULIANA MACIUCA University of Liverpool, School of Veterinary Science
DescriptionPrecisely optimized real time qPCR master mix for probe based assays. This master mix has been developed for TaqMan® probes but is also suitable for other probe types such as Molecular Beacon and Scorpion.
Benefits• Suitable for singleplex and multiplex assays
• High specificity and sensitivity
• Reaction set-up and shipment without dry ice
• One qPCR mix for all cyclers (except capillary)
• Superior results with templates with up to 75% GC content
• Contains dUTP to prevent cross-contamination with UNG treatment
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
Tip!Probe based qPCR is recommended over a dye based approach when
specificity is especially important.
10
Send your sample request to [email protected]
PRODUCT CAT. NO. RXN/20µl SIZE/ml
5x HOT FIREPol® Probe qPCR Mix Plus (ROX)
08-14-0000S (free sample)08-14-0000108-14-0000808-14-00020
5025020005000
0.21820
5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)
08-15-0000S (free sample)08-15-0000108-15-0000808-15-00020
5025020005000
0.21820
5x HOT FIREPol® Probe qPCR Mix Plus (Capillary)
08-16-0000S (free sample)08-16-0000108-16-0000808-16-00020
5025020005000
0.21820
10
0.1
0.0120
1
0.2
22 24 26 28 30 32 34 36 38 40
Del
ta R
n
Cycle Number
10
0.1
0.0120
1
0.2
22 24 26 28 30 32 34 36 38 40
Del
ta R
n
Cycle Number
10
0.1
0.0115
1
0.2
17 19 21 23 25 27 29 31 33
Del
ta R
n
Cycle Number35
10
0.1
0.0120
1
0.2
22 24 26 28 30 32 34 36 38 40
Del
ta R
n
Cycle Number
10
0.1
0.0120
1
0.2
22 24 26 28 30 32 34 36 38 40
Del
ta R
n
Cycle Number
10
0.1
0.0115
1
0.2
17 19 21 23 25 27 29 31 33D
elta
Rn
Cycle Number35
HIGHLY COMPETITIVE
Three tenfold dilutions of 72 bp fragment of Albumin gene were amplified from human genomic DNA using HOT FIREPol® Probe qPCR Mix Plus (red) and a qPCR mix from Company A (green). Reactions were performed on Applied Biosystems 7900HT Real-Time PCR System fol-lowing cycling protocols recommended by the supplier.
MULTIPLEX COMPATIBLE
Amplification of FAM labelled target SNAI1 (orange) and VIC labelled reference gene HPRT (yellow) was performed in a single reaction using HOT FIREPol® Probe qPCR Mix Plus. This multiplex qPCR was carried out on three ten-fold dilutions of human placental cDNA on Applied Bio-systems 7900HT Real-Time PCR System.
5x HOT FIREPol® Probe qPCR Mix Plus
DescriptionCost-effective real time qPCR master mix for probe based qPCR assays. This master mix has been developed for TaqMan® probes but is also suitable for other probe types such as Molecular Beacon and Scorpion.
Benefits• Suitable for singleplex and multiplex assays
• High specificity and sensitivity
• Reaction set-up and shipment without dry ice
• Master mixes for ROX, no ROX and capillary cyclers
Selected publications:
• Vaht, M., et al. International Journal of Neuropsychop-
harmacology (2016).
• Bacakova, M., et al. International journal of nanomedi-
cine (2016).
• Bukovská, P., et al. Frontiers in microbiology (2016).
• Miller, M., et al. J Veterinar Sci Technol (2016).
Researchers already trust Probe Plus
Reference:
“ 5x HOT FIREPol® Probe qPCR Mix Plus (ROX) has been exten-sively used in our lab on a routine basis. Throughout the years, we have successfully analysed thousands of samples using this reliable, optimised, consumer- and budget-friendly solution provided by Solis BioDyne team. ”MARINA GRIGOROVA PhD, Research Fellow of Human Molecular Genetics University of Tartu
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
PRODUCT CATALOGUE 2017 11
Product Selection Guide:Standard PCR Enzymes, Master Mixes and Other Enzymes
Standard PCR Enzymes and Master Mixes
HOT START FIDELITY VS TAQ READY TO LOADAMPLIFICATION
RANGECLONING TYPE ROBUST ON GC PAGE
HOT FIREPol® DNA Polymerase
√ 1× 3 kb TA * * 12
5x HOT FIREPol® GC Master Mix
√ 1× 3 kb TA * * * 13
5x HOT FIREPol® Blend Master Mix
√ 5× 5 kb Blunt * 14-15
5x HOT FIREPol® Blend Master Mix Ready to Load
√ 5× √ 5 kb Blunt * 14-15
FIREPol® DNA Polymerase
1× 3 kb TA * * 16
5x FIREPol® Master Mix
1× 3 kb TA * 17
5x FIREPol® Master Mix Ready to Load
1× √ 3 kb TA * 17
Primer Extension
SPECIFICATION HOT START ddNTPs PAGE
HOT TERMIPolThermostable hot-start DNA Polymerase suited for MALDI-TOF and other primer extension platforms √ √ 18
TERMIPolThermostable DNA Polymerase suited for MALDI-TOF and other primer extension platforms √ 18
Reverse Transcription
SPECIFICATION
ECONOMICAL
CDNA
SYNTHESIS
RNA PROTECTION
FROM RNASE
DEGRADATION
OPTIMAL
WORKING
TEMP.
HIGH
SENSITIVITY
CDNA
COMPATIBLE
WITH QPCR
PAGE
M-MLV RNase H-Reverse Transcriptase RNase H- DNA Polymerase √ √ 37°C √ √ 19
12
HOT FIREPol® DNA Polymerase
Researchers already trust HOT FIREPol®
Reference:
“ We had some problems with the implementation of a protocol, we tried for a long time with different enzymes without any positive results. We tested the HOT FIREPol® and it was the perfect troubleshooting, besides the great technical support received from Solis BioDyne. ”DR MARIA JOSE SUAREZ CIHATA, University of Costa Rica
Send your sample request to [email protected]
PRODUCT CAT. NO. SIZE/U
HOT FIREPol® DNA Polymerase (5 U/µl)
01-02-0000S (free sample)01-02-0050001-02-01000
1005001000
HIGHLY COMPETITIVE
Four genes from human gDNA were amplified in multi-plex reaction using HOT FIREPol® DNA Polymerase (lane 1-2) and two other hot start enzymes from company A (lane 3-4) and company B (lane 5-6). HOT FIREPol® DNA Polymerase performed well with all four genes in both 10x dilutions.
M 1 2 3 4 5 6
3041
bp
68%
2573
bp
69%
2002
bp
72%
1569
bp
72%
719
bp 7
8%Selected publications:
• Herrendorff, R., et al. Journal of Biological Chemistry
(2016).
• Keenan, J. I., et al. Anaerobe (2016).
• Małkiewicz, A., et al. Molecular Biology Reports (2016).
• Tiwari, A., et al. PloS one (2016).
• Kõks, G. et al. The American Journal of Pathology
(2015).
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
DescriptionChemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only after heat treatment which prevents any unspecific polymerase activity at lower temperatures during reaction set-up. HOT FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl
2 and an
additive for difficult templates.
Benefits• increased specificity and sensitivity
• reduced primer dimer formation
• reaction set-up and shipment without dry ice
M 1 2 3 4 5 6
3041
bp
68%
2573
bp
69%
2002
bp
72%
1569
bp
72%
719
bp 7
8%
PRODUCT CATALOGUE 2017 13
5x HOT FIREPol® GC Master Mix
Researchers already trust GC Master Mix
Reference:
“ Solis BioDyne’s highly flexible, customer-centered approach has proven to be invaluable when working on more challenging DNA regions. 5x HOT FIREPol® GC Master Mix is a high-quality and yet cost-effective solution for analyzing polymorphisms embedded in GC-rich regions. ”MARILIIS VAHT PhD student University of Tartu, Estonia
Send your sample request to [email protected]
PRODUCT CAT. NO. RXN/20µl SIZE/ml
5x HOT FIREPol® GC Master Mix
04-33-00S15 (free sample)04-33-0011504-33-02015
252505000
0.1 120
AMPLICONS OF VARIOUS GC-CONTENT
12 GC-rich genes were amplified from human gDNA using HOT FIREPol® GC Master Mix. Template DNA and DMSO were at final concentrations 1ng/µl and 10% respectively. The Master Mix performed well with GC-content up to 79%.
AMPLICONS OF VARIOUS LENGTHS FROM GC-RICH TEMPLATEGC-rich fragments of various length from human gDNA B4GN4 gene were amplified with HOT FIREPol® GC Master Mix. Template DNA and DMSO were at final concentrations 1ng/µl and 10% respectively. The Master Mix performed well up to 3000 bp in fragment length.
M 1 2 3 4 5 6 7 8 9 10 11 12
RET
392b
p 79
%
SOX1
8 79
8 bp
76%
HES
5 75
3bp
69%
ENST
733
bp 7
7%
PO3F
3 79
0bp
78%
Q8N
4 72
7bp
78%
B4G
N4
719b
p 78
%
Q4Z
IN3
763b
p 71
%
NP
758b
p 72
%
JUN
D 7
37bp
64,
6%
BAIP
3 78
8bp
65%
LMX1
737
bp 6
8%
M 1 2 3 4 5
3041
bp
68%
2573
bp
69%
2002
bp
72%
1569
bp
72%
719
bp 7
8%
Publication:
• Maksimov, M., et al. Neuropsychologia (2015).
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
Did you know?GC-rich templates need a special PCR master mix
because GC-rich DNA is more difficult to amplify.
It forms stable secondary structures that resist
denaturing and cause unspecific amplification.
DescriptionPCR master mix that has been developed for working with difficult GC-rich templates and DNA secondary structures. The master mix contains hot-start Taq polymerase HOT FIREPol®, MgCl
2, dNTPs and a special buffer for high yield GC-rich amplification.
Benefits• excellent amplification with templates up to 79% GC content• suitable for templates up to 3 kb• reaction set-up and shipment without dry ice• vials of 100% DMSO and 25 mM MgCl
2 allow flexibility with reaction
optimisation
14
5x HOT FIREPol® Blend Master Mix & Blend Master Mix Ready to Load
AMPLICONS OF VARIOUS LENGTH FROM DIFFERENT TEMPLATESLines 1-4 present an excellent amplification of frag-ments of various length from λ DNA. Lines 5 and 6 show two different amplicons amplified from mouse genomic DNA. All these reactions were carried out using 5x HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl
2.
M 1 2 3 4 5 6
Lane
123456
Template
λ DNA λ DNA λ DNA λ DNA Mouse ge mic DNA Mouse genomic DNA
Amplicon Length
499 bp1003 bp1998 bp4991 bp808 bp3838 bp
DescriptionPrecisely optimized PCR master mix for more demanding PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced performance.
Benefits• increased yield, sensitivity and specificity
• up to 5x higher fidelity
• longer templates (up to 5 kb)
• reduced primer dimer formation
• reaction set-up and shipment without dry ice
Researchers already trust Blend
Reference:
“ After years of using the HOT FIREPol® for our routine colony PCR, we have switched to the Blend Master Mix Ready to Load. It still works perfectly and it is really time saving, especially since the loading dye is already included. ”CÉLINE WINCKLER Gene therapy and animal models for neurodegenerative diseases, Vision Institute, Paris, France
Selected publications:
• Parker, N. R., et al. Scientific reports 6 (2016).
• del Mar Montiel-Rozas, M., et al. Mycorrhiza (2016)
• Kazantseva, J., et al. Scientific Reports 6 (2016).
• Proietti, P., et al. Agriculture, Ecosystems and Environment
(2015).
• Tedersoo L., et al. Science (2014).
Did you know?MgCl2 is needed for polymerase activity. Low MgCl2 concentration
can result in no PCR product, however too high MgCl2
concentration can increase unspecific amplification. The optimal concentration varies
within experiments but usually 1,5-2 mM is sufficient.
PRODUCT CATALOGUE 2017 15
Send your sample request to [email protected]
PRODUCT CAT. NO. RXN/20µl SIZE/ml
5x HOT FIREPol® Blend Master MixReady to Load with 7.5 mM MgCl
2
04-25-00S15 (free sample)04-25-0011504-25-02015
252505000
0.1120
5x HOT FIREPol® Blend Master MixReady to Load with 10 mM MgCl
2
04-25-00S20 (free sample)04-25-0012004-25-02020
252505000
0.1120
5x HOT FIREPol® Blend Master MixReady to Load with 12.5 mM MgCl
2
04-25-00S25 (free sample)04-25-0012504-25-02025
252505000
0.1120
5x HOT FIREPol® Blend Master MixReady to Load with 15 mM MgCl
2
04-25-00S30 (free sample)04-25-0013004-25-02030
252505000
0.1120
Send your sample request to [email protected]
PRODUCT CAT. NO. RXN/20µl SIZE/ml
5x HOT FIREPol® Blend Master Mix with 7.5 mM MgCl
2
04-27-00S15 (free sample)04-27-0011504-27-02015
252505000
0.1120
5x HOT FIREPol® Blend Master Mix with 10mM MgCl
2
04-27-00S20 (free sample)04-27-0012004-27-02020
252505000
0.1120
5x HOT FIREPol® Blend Master Mix with 12.5 mM MgCl
2
04-27-00S25 (free sample)04-27-0012504-27-02025
252505000
0.1120
5x HOT FIREPol® Blend Master Mix with 15 mM MgCl
2
04-27-00S30 (free sample)04-27-0013004-27-02030
252505000
0.1120
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
16
FIREPol® DNA Polymerase
MOUSE GENOMIC DNA
1200 bp fragment of Beta-synuclein gene was amplified from mouse genomic DNA using FIREPol® DNA Polymerase with two different buffers: B (lane 1-3) and BD (lane 4-6). Template DNA was serially diluted tenfold with a starting concentration of 1 ng/µl. FIREPol® DNA Polymerase was used at 0.04U/µl.
PLANT GENOMIC DNA
672 bp fragments was amplified from barley genomic DNA using FIREPol® DNA Polymerase with two buffers: B (lane 1-3) and BD (lane 4-6). Template DNA was serially diluted tenfold with a starting concentration of 1 ng/µl. The enzyme performed well even with the template’s concentration being as low as 0.01 ng/µl. FIREPol® DNA Polymerase was used at 0.04 U/µl.
Send your sample request to [email protected]
PRODUCT CAT. NO. SIZE/U
FIREPol® DNA Polymerase (5 U/µl)
01-01-0000S (free sample)01-01-0050001-01-0100001-01-02000
10050010002000
M 1 2 3 4 5 6
M 1 2 3 4 5 6
M 1 2 3 4 5 6
M 1 2 3 4 5 6
Researchers already trust FIREPol®
Reference:
“ I found that for FIREPol® DNA Polymerase the quality was comparable to similar products even though the price was much cheaper for the Solis product. Therefore, Solis BioDyne are head and shoulders above their competitors when it comes to value for money which is especially important given the funding situation in these straitened times. ”DR. GARY LOUGHRAN Research Fellow School of Biochemistry and Cell Biology University College Cork, Ireland
Selected publications:
• Corte, L., et al. Scientific Reports (2016).
• Lawrence, S. A., and Poulin, R., Parasitology
Research (2016).
• Pilkington, K. M., and V. Vaughan Symonds. Applications
in Plant Sciences (2016).
• Tockner, B., et al. Gene Therapy (2016).
• Jopcik, M., et al. Molecular Biology (2015).
• Corte, L., et al. Food Microbiology (2015).
DescriptionGenetically modified thermostable Taq DNA polymerase that pro-vides robust and reproducible results. FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl
2 and an additive for
difficult templates.
Benefits• robust amplification for routine applications
• suitable for templates up to 3 kb
• reaction set-up and shipment without dry ice
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
PRODUCT CATALOGUE 2017 17
Researchers already trust FIREPol® Master Mix Reference:
“ We are working with Firepol® Mastermix for few years now and we are very satisfied. We are using it for genotyping (many different PCR) and we have excellent results. The ready to load format is very time saving. ”CLAUDINE CORNELOUP Engineer assistant Platform for Experimental Biology on Mice, Genotyping Laboratory, ENS de Lyon
5x FIREPol® Master Mix & Master Mix Ready to Load
DescriptionOptimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl
2, dNTPs and buffer with detergent. You just
need to add template, primers and water.
Benefits• ready to load version allows you to load the PCR product on your gel straight after cycling
• reaction set-up and shipment without dry ice
• all-in-one master mix format reduces pipetting errors and saves time
Send your sample request to [email protected]
PRODUCT CAT. NO. RXN/20µl SIZE/ml
5x FIREPol® Master Mix with 7.5 mM MgCl2
04-11-00S15 (free sample)04-11-00115
25250
0.11
5x FIREPol® Master Mix with 12.5 mM MgCl2 04-11-00S25 (free sample)
04-11-0012525250
0.11
5x FIREPol® Master Mix Ready to Load with 7.5 mM MgCl
2
04-12-00S15 (free sample)04-12-00115
25250
0.11
5x FIREPol® Master Mix Ready to Load with 12.5 mM MgCl
2
04-12-00S25 (free sample)04-12-00125
25250
0.11
PLANT GENOMIC DNA
672 bp fragment was amplified from barley genomic DNA using FIREPol Master Mix (lane 1-3) and FIREPol Master Mix Ready to Load (lane 4-6). Template DNA was serially diluted tenfold from a starting concentration of 1 ng/µl. The Master Mixes performed well even with the template’s concentration being as low as 0.01 ng/µl.
M 1 2 3 4 5 6
Selected publications:
• Mohammadi, S. M., et al. Neuropeptides
(2016).
• Pooljun, C., et al. Aquaculture (2016).
• Purahong, W., et al. Fungal Ecology (2016).
• Trüeb, B., et al. Veterinary Microbiology
(2016).
• Al-Mohaya, M.A.M., et al. BMC Oral Health
(2016).
• Rasheed, F., et al. Heliobacter (2014).
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
18
Researchers already trust TERMIPol® Reference:
“ Our group is using the TERMIPol® already for 10 years for primer extension
reactions with subsequent HPLC separation. Compared to similar products on
the market TERMIPol® incorporates ddNTPs with high efficiency and low error
rates. We highly recommend using this enzyme for SNP genotyping or bisulfite-
based single CpG screening, as low as 1.25 U are sufficient per reaction. Since no
detergents are used in storage and reaction buffers, primer extension reactions
can be loaded unpurified on HPLC systems which saves time and costs. We are
using this enzyme frequently and experienced TERMIPol® as robust and reliable
enzyme offering highly efficient and reproducible results. ”DR. SASCHA TIERLING Universität des Saarlandes, GERMANY
Send your sample request to [email protected]
PRODUCT CAT. NO. SIZE/U
TERMIPol® DNA Polymerase (5 U/µl)
01-03-0000S (free sample)01-03-0050001-03-02000
5005002000
HOT TERMIPol® DNA Polymerase (5 U/µl)
01-06-0000S (free sample)01-06-0050001-06-02000
5005002000
TERMIPol® DNA Polymerase
DescriptionThermostable DNA polymerase that has an increased efficiency for incorpo-rating unconventional nucleotides such as ddNTPs, acyNTPs or fluorescent nucleotides. TERMIPol® DNA Polymerase is supplied with a reaction buffer and 100 mM MgCl
2.
Benefits• high efficiency for incorporating unconventional nucleotides
• assay success rate of 99% in MALDI-TOF
• robust and reliable
• reaction set-up and shipment without dry ice
Selected publications:
• Royo, J.L., et al. Molecular and Cellular Probes (2015).
• Gorokhova, S.G., et al. J. Clin Exp Cardiolog (2014).
• Thorkildsen, L.T., et al. Gastroenterology Research and
Practice (2013).
• Ilina, E.N., et al. Front Microbiol (2013).
• Tierling, S., et al. International Journal of Cancer (2012).
Did you know?The ability to incorporate
unconventional nucleotides makes TERMIPol suitable for
primer extension, MassARRAY and MALDI-TOF mass
spectrometry.
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
PRODUCT CATALOGUE 2017 19
M-MLV Reverse Transcriptase RNase H- DNA Polymerase
DescriptionGenetically modified M-MLV reverse transcriptase with RNA or DNA dependent DNA polymerase activity but lacking RNase H activity. The optimal reaction temperature for this enzyme is 37oC. The kit contains M-MLV RT RNase H-, 2 buffers, vials of 25 mM MgCl
2, 20 mM
MnCl2 and 100 mM DTT.
Benefits• Cost-effective enzyme for cDNA synthesis
• cDNA is compatible with downstream qPCR applications
• No RNase H activity
cDNA
RNase
RNase H degrades mRNA, causing truncation of cDNA
mRNAA
T
A
T
A
T
ReverseTranscriptase
cDNA
mRNAA
T
A
T
A
T
ReverseTranscriptase
Removal of the RNase H activity results in an increase of full-length cDNA products
RNASE H ACTIVITY
Researchers already trust M-MLV H- Reference:
“ Our research group has been using Solis BioDyne's M-MLV Reverse Transcriptase in gene expression analysis by RT-qPCR for many years. The enzyme yields reproducible results with high sensitivity and is affordably priced. ”TÕNIS ÖRD PhD, senior research scientist Estonian Biocentre
Selected publications:
• Giordani, T., et al. Tree Genetics & Genomes (2016).
• Raba, M., et al. Frontiers in Cellular Neuroscience
(2016).
• Wen, B., et al. Plos ONE (2014).
• Örd, T., et al. Cellular Immunology (2012).
• Kesireddy, V., et al. Molecular Biology Reports (2010).
Send your sample request to [email protected]
PRODUCT CAT. NO. SIZE/U
M-MLV Reverse Transcriptase RNase H- (200 U/µl)
06-21-00000S (free sample)06-21-01000006-21-050000
20001000050000
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
20
dNTP Mix and Set
dATPFormula: C
10H
12N
5O
12P
3 (Anion)
Formula Weight: 487.18 (Anion)
dGTPFormula: C
10H
12N
5O
13P
3 (Anion)
Formula Weight: 503.18 (Anion)
dTTPFormula: C
10H
12N
2O
14P
3 (Anion)
Formula Weight: 478.16 (Anion)
dCTPFormula: C
9H
12N
3O
13P
3 (Anion)
Formula Weight: 463.15 (Anion)
O
O
OO-O-
O O
O
O
N
N NP P P
O OO- O-
NH
OH
NH2
O
O
OO-O-
O O
P P PO O
O- O- OON
N
NH2
OH
O
OO-O-
O
O
OO
P P PO O
O- O-
O
N
OH
NHH
3C
O
O
OO-O-
O O
O
N
N N
NP P P
O OO- O-
OH
NH2
Send your sample request to [email protected]
PRODUCT CAT. NO. SIZE/µmol
dNTP Set02-21-0001S (free sample)02-21-0010002-21-00400
4x14x254x100
dNTP Mix02-31-0001S (free sample)02-31-0002002-31-00100
0.820100
dUTP02-41-0000S (free sample)02-41-00025
2.525
“ In 2005 we started to use the Solis Biodyne dNTP Set in our lab. Comparing the performance of Solis Biodyne dNTPs with two other suppliers in a mutation detection assay, we found similar or even higher FRET signals in our analysed samples. Since then, we use the Solis Biodyne dNTP Set in our lab in a wide range of DNA and RNA amplification techniques like end point PCR, mutation detection in FRET assays, qPCR, high resolution melting analysis etc. ”JUERGEN SIEVERTSEN Bernhard Nocht Institute for Tropical Medicine (BNITM), Germany
DescriptionSolis BioDyne's dNTPs are chemically synthesised and have 99% purity determined by HPLC. You can use our dNTPs for a wide range of molecular biology applications.
dNTP SetSeparate vials of dATP, dTTP, dGTP and dCTP at 100 mM concentration.
dNTP MixOne solution of dATP, dTTP, dGTP and dCTP at 20 mM concentration each.
dUTPdUTP is available in a separate vial with a concentration of 100 mM.
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
PRODUCT CATALOGUE 2017 21
100 bp DNA Ladder 1 kb DNA Ladder
Send your sample request to [email protected]
PRODUCT CAT. NO. SIZE
100 bp DNA LadderReady to Load
07-11-0000S (free sample)07-11-00050
1.5 µg50 µg
1 kb DNA LadderReady to Load
07-12-0000S (free sample)07-12-00050
1.5 µg50 µg
6x DNA Loading Dye Buffer Blue
07-01-0000S (free sample)07-01-0000107-01-00010
0.1 ml1 ml10 ml
6x DNA Loading Dye Buffer Double Blue
07-02-0000S (free sample)07-02-0000107-02-00010
0.1 ml1 ml10 ml
6x DNA Loading Dye Buffer Orange and Blue
07-03-0000S (free sample)07-03-0000107-03-00010
0.1 ml1 ml10 ml
6x DNA Loading Dye Buffer Orange
07-04-0000S (free sample)07-04-0000107-04-00010
0.1 ml1 ml10 ml
1 KB DNA LADDER 100 BP DNA LADDER
Lane
1234
DNA Loading Dye Buffer
BlueDouble BlueOrange and BlueOrange
1 2 3 4
Lane
1234
DNA Loading Dye Buffer
BlueDouble BlueOrange and BlueOrange
1 2 3 4 LOADING DYE BUFFERS
In 1% agarose gel 1x TBE, Xylene Cyanol FF migrates along with~3500 bp frag-ments, Bromophenol Blue migrates along with ~300 bp fragments and Orange G migrates along with ~40 bp fragments.
-10,000 80
-3,000 120
-5,000 80
bp ng/10µl
-8,000 80
-4,000 80
-2,500 123
-2,000 81
-1,500 73
-1,000 41
-700 57
-500 61
-300 44
-3,000 100
bp ng/10µl
-2,000 134
-1,500 100
-1,000 104
-800 83-900 93
-700 73-600 63-500 104-400 42-300 31
-200 42
-100 31
1 kb DNA Ladder 100 bp DNA Ladder
6x DNA Loading Dye Buffers
Description6x DNA Loading Dye Buffers are used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. The optimized solutions contain different mixtures of three dyes: Bromophenol Blue, Xylene Cyanol FF and Orange G for visual tracking of DNA migration during electrophoresis.
6x DNA Loading Dye Buffers containing Orange G are recommended for the analysis of small DNA molecules and have no DNA masking during gel exposure to UV light. 6x DNA Loading Dye Buffer Blue and Dye Buffer Double Blue make pipetting visually easy with its dark blue color.
DescriptionSolis BioDyne DNA ladders are convenient ready-to-use molecular weight markers for DNA fragment size determination on gel electrophoresis. The ladders are supplied in a loading buffer and are stable at ambient temperature. The 1 kb DNA Ladder contains 13 discrete DNA fragments ranging from 300 bp to 10,000 bp. The 100 bp DNA Ladder contains 13 discrete DNA fragments ranging from 100 bp to 3,000 bp.
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
22
Send your sample request to [email protected]
PRODUCT CAT. NO. SIZE/ml
10x GC-rich Enhancer
05-16-0000S (free sample)05-16-0001005-16-0005005-16-00200
0.11520
25 mM MgCl2
05-11-0002505-11-0005005-11-00200
2.5520
PCR Grade Waterwater-025water-100water-500
25100500
10x GC-rich EnhancerDescription10x GC-rich Enhancer is used as PCR additive for difficult GC-rich templates. The optimized solution modifies melting behavior of nucleic acids and often enhances amplification of suboptimal PCR systems with high degree of secondary structures and GC-rich regions.
10x GC-rich Enhancer should be used at a defined working concentration (1x, 2x or 3x solution) and only if non-specific amplification occurs.
Applications• Additive for PCR reaction
25 mM MgCl2
DescriptionMagnesium Chloride (MgCl
2) is an important component of PCR reactions. Concentration
of MgCl2 should be optimized according to reaction conditions (primer, template, dNTP,
polymerase concentration).
Applications• Optimization of PCR, qPCR and RT-PCR reactions• All other molecular biology techniques where MgCl
2 is needed
PCR Grade WaterDescriptionPCR Grade Water is deionized and autoclaved water suitable for use in all experiments that require nuclease-free water. PCR Grade Water is prepared without chemical additives and it is DNase, RNase and nuclease-free.
Applications• PCR, qPCR and RT-PCR • All other molecular biology techniques where pure water is needed
Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee
PRODUCT CATALOGUE 2017 23
How to OrderOrders can be placed:
• Via E-Shop: www.sbd.ee• By emailing: [email protected]• With your account manager or local distributor• Via fax: +372 740 2079
Required Information
Following information is required while placing an order:
• Shipping and invoice address• Contact person's name and phone number• VAT number (EU only)• Product name and corresponding catalogue number
Shipping Cost
Depending on the order amount a shipping cost may be added to the invoice. Please contact us for shipping cost quotation.
Customer Care
We are committed to providing our customers excellent service. All inquiries will be responded to within 1 business day at most. All technical questions will be given high priority and our full attention.
Please contact us through Skype: support.sbd or via e-mail: [email protected]
Customized solutions
This product catalogue contains standard products, tube sizes and kits. Please contact us for customized solutions.
Shipping
Unless agreed otherwise, all shipments abroad will be arranged via express courier service. Orders are confirmed generally within 1 business day (Monday to Friday, 8AM to 5PM, UTC+2) after reception. In most cases orders are shipped within 1 or 2 business days.
Delivery documents and other charges
For non-EU shipments, please inform us of the documents required for shipments to your country. Solis BioDyne is not liable for import duties and taxes or delays caused by the brokerage procedure or other third parties.
Payment Options
Solis BioDyne accepts payments by:
• Wire transfer, based on invoice• PayPal, based on invoice or for orders placed through e-shop• Credit Card (VISA or Master Card) for orders placed through
our e-shop
Checks are not accepted as a payment method.
Please see our full ordering conditions on www.sbd.ee
All Solis BioDyne products are shipped at ambient temperature.
Our products can withstand ambient temperature up to 1 month without any loss of activity. However, regular storage at -20°C is recommended.
Ordering
Free samplesSolis BioDyne provides free samples of the entire product range enabling our clients to thoroughly test our products.
24
We are based in Estonia, a member of the European Union and €-zone
International presence
International PresenceSolis BioDyne currently has clients in 110 countries. We make our reagents accessible globally either by supplying them directly or in some areas by relying on local distributors who share our high standards in service and technical support. Please see the distributors section on next page or contact us directly to find the most convenient way to order in your region.
PRODUCT CATALOGUE 2017 25
DistributorsAFRICA
DELTA Trading & Development Egypt T: +20 2 3573 6679 E: [email protected] www.deltatd.com
SeparationsSouth AfricaT: +27 11 919 1000 E: [email protected]
ASIA
Biogenuix Medsystems Pvt. Ltd. India T: +91 11 2561 2008E: [email protected] www.biogenuix.com
PT. Sentra Biosains DinamikaIndonesia T: +62 21 4288 6574 E: [email protected] www.sentrabd.com
Cosmo Bio Co., Ltd.JapanP: +813 5632 9610E: [email protected]
Next Gene ScientificMalaysiaT: +603 5882 8650E: [email protected] www.nextgene.my
Med Lab ServicesPakistanT: +92 51 4419341 E: [email protected]
Genomicbase Republic of Korea T: +82 2 2215 4925E: [email protected]
TLC Scientific Solutions Pte. Ltd.SingaporeT: +656464 0261E: [email protected]
Hongblue Life Science Trade Co. Taiwan T: +886 4 2263 1262E: [email protected] www.hongblue.url.tw
Life Science AP Co., LtdThailandT: +66 81 8296282E: [email protected]
EUROPE
Medibena Austria T: +43 1 9906 497 E: [email protected] www.medibena.at
Laborimpex SABelgiumT: +32 2 345 99 94E: [email protected]
BariaCzech Republic T: +420 774 227 421 F: +420 244 911 2 28 E: [email protected] www.baria.cz
TAG Copenhagen A/SDenmarkT: +45 321 322 00 E: [email protected]
LabnetFinland T: +358 20 741 31 70 F: +358 20 741 31 89 E: [email protected] www.labnet.fi
BioinnotechGreeceT: +30 210 51 41 746www.bioinnotech.gr
Bio-KasztelHungaryT: +36 1 381 0694E: [email protected] www.kasztel.hu
Euroclone S.P.AItalyT: +39 02 38.19.51E: [email protected]
SIA AdronaLatviaT: +371 67551894E: [email protected]
Bio-Connect B.V.The NetherlandsT: +31 26 3264 450E: [email protected]
Cytogen Polska Sp. z o.o. Poland T: +48 42 6300 598 E: [email protected] www.cytogen.com.pl
DAGMA, Lda. Portugal T: +351 21 4573 222 E: [email protected]
Bio Zyme SRLRomaniaT: +40 264 523281E: [email protected]
Ecoli s.r.o. SlovakiaT: +421 0264 789 336 E: [email protected] www.ecoli.sk
Genycell BiotechSpainT: +34 902 194353E: [email protected]
LucernaChem AGSwitzerlandT: +41 41 4209 636E: [email protected]
BM Yazılım Danıs. ve Laboratory Systems Ltd. Sti. Turkey T: +90 312 4472 280 E: [email protected] www.bmlabosis.com
Newmarket Scientific United Kingdom T: +44 (0)1638 551500 E: [email protected] www.newmarketscientific.com
Tespro LLCUkraineT: +380 44 220 110 5E: [email protected]
OCEANIA
Integrated Sciences Pty. Ltd. AustraliaT: +61 2 9417 7866 E: [email protected] www.integratedsci.com.au
dnatureNew ZealandT (toll-free): 0800 362 8873E: [email protected]
MIDDLE EAST
BioConsult Israel T: +972 2 5667 043 E: [email protected] www.bioconsult.co.il
Pishgam Biotech CompanyIranT: +98 21 8801 4393E: [email protected]
Al Genome Medical CompanyJordanT: + 962 652 33 670E: [email protected]
Al Genome International Scientific and Laboratory ProductsKingdom of Saudi ArabiaUnited Arab Emirates T: +971557011398E: [email protected]
Med CloneKuwaitT: +965 23915602E: [email protected]
NORTH AMERICA
Mango Biotechnology LLC USA T: +1 650 5758 657 E: [email protected] www.mangobio.com
SOUTH AMERICA
Biocientifica S.A. ArgentinaT: (54-11) 4857-5005E: [email protected]
Sinapse Biotecnologia LtdaBrazilT: +55-11-2605-5655E: sinapse@sinapsebiotecnologia.com.brwww.sinapsebiotecnologia.com.br
Fermelo Biotec Chile T/F: +562 2247 2978E: [email protected]
SurGenoma S.A.S. ColombiaT: +57 1 3441325E: [email protected]
The most up-to-date list of distributors is available on our website www.sbd.eei
26
qPCR Mixes
CAT. NO. SIZE
5x HOT FIREPol® EvaGreen® qPCR Supermix
08-36-0000S08-36-0000108-36-0000808-36-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)
08-24-0000S08-24-0000108-24-0000808-24-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
5x HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX)
08-25-0000S08-25-0000108-25-0000808-25-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
5x HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary)
08-26-0000S08-26-0000108-26-0000808-26-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
5x HOT FIREPol® EvaGreen® HRM Mix (ROX)
08-33-0000S08-33-0000108-33-0000808-33-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
5x HOT FIREPol® EvaGreen® HRM Mix (no ROX)
08-31-0000S08-31-0000108-31-0000808-31-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
5x HOT FIREPol® Probe Universal qPCR Mix
08-17-0000S08-17-0000108-17-0000808-17-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
5x HOT FIREPolR Probe qPCR Mix Plus (ROX)
08-14-0000S08-14-0000108-14-0000808-14-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)
08-15-0000S08-15-0000108-15-0000808-15-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
5x HOT FIREPol® Probe qPCR Mix Plus (Capillary)
08-16-0000S08-16-0000108-16-0000808-16-00020
50 rxn/20 µl250 rxn/20 µl
2000 rxn/20 µl5000 rxn/20 µl
Regular PCR Enzyme and Mixes
CAT. NO. SIZE
FIREPol® DNA Polymerase (5 U/µl)
01-01-0000S 01-01-00500 01-01-01000 01-01-02000
100 U 500 U
1000 U 2000 U
5x FIREPol® Master Mix with 7,5 mM MgCl2
04-11-00S15 04-11-00115
25 rxn/20 µl 250 rxn/20 µl
5x FIREPol® Master Mix with 12.5 mM MgCl2
04-11-00S25 04-11-00125
25 rxn/20 µl 250 rxn/20 µl
5x FIREPol® Master Mix Ready to Load with 7,5 mM MgCl2
04-12-00S15 04-12-00115
25 rxn/20 µl 250 rxn/20 µl
5x FIREPol® Master Mix Ready to Load with 12,5 mM MgCl2
04-12-00S25 04-12-00125
25rxn/20 µl 250 rxn/20 µl
Other Enzymes
CAT. NO. SIZE
TERMIPol® DNA Polymerase (5 U/µl) 01-03-0000S01-03-0050001-03-02000
500 U500 U
2000 U
HOT TERMIPol® DNA Polymerase (5 U/µl) 01-06-0000S01-06-0050001-06-02000
500 U500 U
2000 U
M-MLV Reverse Transcriptase RNase H- (200 U/µl) 06-21-00000S06-21-01000006-21-050000
2000 U10000 U50000 U
Product List
PRODUCT CATALOGUE 2017 27
Hot-start PCR Enzyme and Mixes
CAT. NO. SIZE
HOT FIREPol DNA Polymerase (5U/µl)01-02-0000S01-02-0050001-02-01000
100 U500 U
1000 U
5x HOT FIREPol® GC Master Mix 04-33-00S1504-33-0011504-33-02015
25 rxn/20 µl250 rxn/20 µl
5000 rxn/20 µl
5x HOT FIREPol® Blend Master Mix with 7.5 mM MgCl
2
04-27-00S1504-27-0011504-27-02015
25 rxn/20 µl250 rxn/20 µl
5000 rxn/20 µl
5x HOT FIREPol® Blend Master Mix with 10 mM MgCl
2
04-27-00S2004-27-0012004-27-02020
25 rxn/20 µl250 rxn/20 µl
5000 rxn/20 µl
5x HOT FIREPol® Blend Master Mix with 12.5 mM MgCl
2
04-27-00S2504-27-0012504-27-02025
25 rxn/20 µl250 rxn/20 µl
5000 rxn/20 µl
5x HOT FIREPol® Blend Master Mix with 15 mM MgCl
2
04-27-00S3004-27-0013004-27-02030
25 rxn/20 µl250 rxn/20 µl
5000 rxn/20 µl
5x HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl
2
04-25-00S1504-25-0011504-25-02015
25 rxn/20 µl250 rxn/20 µl
5000 rxn/20 µl
5x HOT FIREPol® Blend Master Mix Ready to Load with 10 mM MgCl
2
04-25-00S2004-25-0012004-25-02020
25 rxn/20 µl250 rxn/20 µl
5000 rxn/20 µl
5x HOT FIREPol® Blend Master Mix Ready to Load with 12.5 mM MgCl
2
04-25-00S2504-25-0012504-25-02025
25 rxn/20 µl250 rxn/20 µl
5000 rxn/20 µl
5x HOT FIREPol® Blend Master Mix Ready to Load with 15 mM MgCl
2
04-25-00S3004-25-0013004-25-02030
25 rxn/20 µl250 rxn/20 µl
5000 rxn/20 µl
Other PCR Reagents
CAT. NO. SIZE
dNTP Set02-21-0001S 02-21-00100 02-21-00400
4x1 µmol 4x25 µmol
4x100 µmol
dNTP Mix02-31-0001S 02-31-00020 02-31-00100
0.8 µmol 20 µmol
100 µmol
dUTP 02-41-0000S 02-41-00025
2.5 µmol 25 µmol
100 bp DNA Ladder Ready to Load 07-11-0000S 07-11-00050
1.5 µg 50 µg
1 kb DNA Ladder Ready to Load07-12-0000S 07-12-00050
1.5 µg 50 µg
6x DNA Loading Dye Buffer Blue07-01-0000S 07-01-00001 07-01-00010
0.1 ml 1 ml
10 ml
6x DNA Loading Dye Buffer Double Blue07-02-0000S 07-02-00001 07-02-00010
0.1 ml 1 ml
10 ml
6x DNA Loading Dye Buffer Orange and Blue07-03-0000S 07-03-00001 07-03-00010
0.1 ml 1 ml
10 ml
6x DNA Loading Dye Buffer Orange07-04-0000S 07-04-00001 07-04-00010
0.1 ml 1 ml
10 ml
10x GC-rich Enhancer
05-16-0000S 05-16-00010 05-16-00050 05-16-00200
0.1 ml 1 ml 5 ml
20 ml
25 mM MgCl2
05-11-00025 05-11-00050 05-11-00200
2.5 ml 5 ml
20 ml
PCR Grade Waterwater-025water-100water-500
25 ml100 ml500 ml
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olis
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