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Team SDU-Denmark
Us at Harvard(of course we like
MIT too)
The idea
Our goal is to create a
bandage, which inhibits S.
aureus biofilm formation in wounds.
Such a bandage would
improve the efficiency of conventional antibiotics.
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The problem
Hospital acquired infections
are a huge and growing
problem.
They lead to prolonged illness and higher mortality
among patients.
S. aureus is the leading cause of hospital-acquired
infections.
The problem
S. aureus often infects
surgical wounds.
This disrupts the healing process, can cause
sepsis and may require implanted devices to be removed.
The increased use of antibiotics promotes
resistance as seen with MRSA.
Biofilm formation
Biofilm is a layer of microbes embedded in
extracellular slime.
Biofilm formation in wounds protects
S. aureus from antibiotics.
Quorum sensing enables bacteria
to form biofilms.
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Quorum sensing
S. aureus and other Gram positives
agrCagrA RNAIII
AgrC
P2
AgrA
P3
RNAIII+
AIP
agrD agrB
AgrB
AgrD
+
Our system
S. Aureus
Infection
Antibiotics
Resistance
Biofilm
Quorum
sensingSensing
RIP
+
AgrC
AgrA
RIP
AIPE. coli
RIPcon. pro. P3agrA agrC
S. aureusOur system
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A bandage containing E. coli producing RIP, sealed in
a semipermeable membrane.
The membrane prevents the bacteria from entering
the blood, but allows RIP to diffuse into the wound and disrupt quorum sensing.
E. coli
Membrane
Cover
RIPAIP
Wound
BactoBandage
Constitutive promotor
RBS RIP Double terminator
Our system
DNA sequencing
Restriction-sites Composite promoter and RBS RIP Double terminator
Results
Northern blot
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Results
0,00
0,10
0,20
0,30
0,40
0,50
0,60
Cell culture Supernatant Lysate
No
rmali
zed
ab
so
rban
ce
MG1655 + RIP
MG1655
Biofilm assay
In conclusion
What we achieved
� RIP submitted part
� RIP mRNA confirmed by Northern blot
� S. aureus biofilm formation reduced by RIP cell culture
�First expression of RIP outside original host
Acknowledgements
Fabulously fantastic advisors &
University of Southern Denmark