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PPR technology
1
4ii
PPR motif (35 aa)v t Y n t l I s g l c k a G r l e e A l e l f e e M k e k - G i a P d v1 4 ii
- A - G - U - C - A - C - U - G - A - G - A -
PPR protein
RNA
Base
Human mitochondrial RNA pol.Ringel et al., Nature (2011)
FTN VNS VTD FPD(1,4,ii)
Rec. base
[Base recognition code]
&��#* (�RNA/DNA�� ��$��"%!����)-�',��(�������������)+����
Nu
ER
mRNA
Protein
???
RNA virus
mitochondria
DNAncRNA
Pentatricopeptide repeat
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����
l Dhl ��/ / :
5 ��� ) �������������
l N (����
l N (� � .
l (2 R( /R( /R(
l (l 0
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3 P5
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Vision ~ New Tools Lead to a New World~
< A D >6 :C : 167: 5 D A < >
N PR P ab _ JJ
3CA :
02.
42.
42.
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/:>>
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3. Genome editing (since 2010)• ゲノムを自在に書き換える。
Science Breakthrough of the year 2013, 2014, 2015
Break-through technologies for the cell engineering
NGS
G.E.AI
1K
1M
1G
1T
Proc
essi
ng s
peed
/day
1985 1990 1995 2000 2005 2010
←Human Genome (3 G bp)
Manual seq.
ABI377
ABI3700
ABI3730
GS20(454)
GAI(Solexa)
SOLiD
SOLiD3
Heliscope
GAII(Solexa)
FLX Titanium
PacBio
1. 大規模配列解析技術(since 2004)ヒトゲノム : 1日で解読
• 多様な生物のゲノム情報を理解• 病気などの異常によるゲノム情報変化を理解
AlphaGo by Google DeepMind
2. 人工知能 (since 2015)• ゲノム情報改変の理論を理解• 遺伝子回路の構築原理を理解
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KHDACI� TBJU=��
��
�#��@��
����+9OQGL<��#@-$
�� B�� A
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�� B
�;�:��@4683
&%=�#=>@�7��@/�
10�
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EKL)2MFNSP@��
�0
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EKL)2
�0
RRR
RRR
���1=*�3�,@."95?! (=��http://www.asahi.com/articles/photo/AS20150326000124.html
Genome editing (�����)���� (�� '&��$)�������������'&��( !�#����������"%�
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AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGTAATACGACTCACTATAGGGCAAGCTTAAAAAGCCTTCCATTTTCTATTTTGATTTGTAGAAAACTAGTGTGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCTACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGCGACTCTAGAATTCCAACTGAGCGCCGGTCGCTACCATTACCAACTTGTCTGGTGTCAAAAATAATAGGCCTACTAGTCGGCCGTACGGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGGCCTTAAGGGCCTCGTTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTT
��-0) �+��.8��BDNAC
DNA54�%BA, T, G, C)
@ 1 �1��→ 1/44��
@ 3 �1��→ 1/4 & 1/4 & 1/4 (1/4n; n=3C4��'�1/644��
;=>B30� �C*71�:��.835'
1/416= 1/42�4��
→16 �1��.96�)��328(
/4�'DNA:��B��D!�:��C(
;=>�$")DNA �:��3�1,8<A?4#�
���� = �� �����
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���"DNA��,6/)�
* * * *A T T C
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���#� = &��$ �'��"
DSB
Donor DNA (~16 kb)
Donor DNA(ssDNA, 100b)
NHEJ#�� � HR#�� �
DSB: double strand breakNHEJ: non homologous end joining, �������HR: homologous recombination, ���"��
**��$���
(no-GMO?)ZFN-1
+���#��
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(no-GMO?)ZFN-2
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(GMO)ZFN-3
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G i y MGF T F M G FML E
T v v E
-
P v E
-r c
c F ML E
T t e lh a
o s A
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p• i e• e
S 2oP A
a 2
Val83, Ser85, Asp87 and Thr89), with each protomer contributingthree residues. Because many of the interactions are redundant,recognition of A(–7) will be described in detail and only thedifferences found in the A+1 binding pocket will be highlighted.For clarity, the prime symbol denotes residues located on the oppositeprotomer of the PP7DFG dimer.
The upper surface of the A(–7) binding pocket is formed by thehydrophobic side chain of Val83¢ and the aliphatic portion of theLys58¢ side chain, which make van der Waals contacts with the base(Fig. 2b,c). In the A+1 pocket, the side chain amine of Lys58 hydrogenbonds to the 2¢OH of G+3 and likely stabilizes its C2¢-endo sugarpucker (Fig. 2c). Asp87, Thr89 and Ser85¢ form the middle level of thepocket and make sequence-specific contacts to the adenine base. Theside chain OH of Thr89 is within hydrogen-bonding distance of boththe adenine N7 and N6 exocyclic amines. The backbone carbonyl ofAsp87 is also within hydrogen-bonding distance of the N6 exocyclicamine. The third RNA-protein interaction at this level is a hydrogenbond between the OH of Ser85¢ and adenine N1. Arg54, whoseguanidinium group makes a cation-p stacking interaction with theadenine base, forms the base of the binding pocket. The position of theArg54 side chain is buttressed by hydrogen bonds to the side chain ofAsp87. In the A+1 pocket, the Arg54¢ guanidinium group makes twohydrogen bonds to O6 and N7 of G+3 to specifically recognize theguanine base. (Fig. 2c). The importance of Lys58 and Arg54 in RNArecognition is supported by experiments that demonstrate that muta-tion of these residues leads to severe repression defects4.
The PP7DFG–RNA complex is further stabilized by several hydro-gen bonds and electrostatic interactions outside of the adeninerecognition pockets. The backbone amide of Gly48¢ and the sidechain of Asn47¢ make hydrogen bonds to phosphate oxygens of A(–2)and U(–1), respectively. The U(–1) nucleotide is extended away fromthe loop into a pocket formed by Thr51¢, Ala52¢, Val91¢ and Thr81.The backbone amide and side chain OH of Thr81 make hydrogenbonds to the O2 and 2¢-OH of U(–1). Another potential hydrogenbond exists between the side chain carboxylic acid of Asp60 and theA+1 O2¢, which may stabilize its C2¢-endo sugar pucker. A similarinteraction is observed in the MS2 coat protein–RNA complex, whereGlu63 is hydrogen bonded to the U(–5) 2¢ OH13. Crystal-packing
differences result in the side chain of Arg24 stacking with the guaninebase of G+4 and contacting either its O4 or phosphate oxygens. Weakelectron density and alternate conformations of the Arg24 side chainsuggest that this interaction does not contribute substantially to theoverall affinity of complex. There are also several positively chargedresidues (Arg24¢, Arg39, Arg45¢) that may participate in favorableelectrostatic interactions with the phosphate backbone of the RNA.
Although the PP7 and MS2 coat proteins share similar proteinscaffolds, their RNA-binding surfaces have evolved to specificallyrecognize distinct RNA hairpins. The most notable difference betweenthe two structures is the location of the adenine-recognition pockets,which are important components of binding for both coat pro-teins12,14,15. In the PP7 coat protein, the pockets are aligned along
b c
5′5′ 3′3′
3′
5′
G - C
A - U
G - C
A - UA - U
C - G
A
AU G
A U
C - G
G - CG - C
G
G - C
G - C
G - C
U - A
C - GA - U
A - U
A
AU U
A
N
N
NN
C
C
C C
–14–13
–12
–11
–10 –9
–8
–7
–6
–5
–4–3
–2–1
+1
+2+3
+1+2
+3
+4+5
+6
+7+8
+9
+10+11
+12+13
–2
–3–4
–5
–6
–7–8
–9–10
–11
–12
–1
MS2GA
1 10 20 30 40 50 60 70 80 90 100 110 120
QbPP7
a
* * **
+1
3′5′
+1Figure 1 Coat-protein sequencealignment and overview of the MS2coat protein and PP7DFG complexeswith RNA. (a) Alignment of four ssRNAbacteriophage coat proteins. (b,c) MS2coat protein–RNA (2BU1) (b) andPP7DFG–RNA (c) complexes areshown as cartoons. In both structuresthe RNA hairpin (orange, adenine; red,guanine; violet, uridine; yellow,cytidine) binds across the extendedb-sheet surface formed by the coatprotein dimer (blue, green).
b
cVal83
V83
A(–7)
A(–2)
G(–3)
A(–4)
A(–5)
G(–6)
A(–9)
G(–11)
G(–12)
C(–8)
K58
G48
N47
S85
T89
T81
U(–1)
U+2
D87 R54
R54
D60
S85 D87
A+1
G+4
C+5
G+3
U+6
U+7
C+8
G+9
U+10
G+11
C+12
C+13
V83
T89
K58
Ser85
Lys58
Arg54
Asp87Thr89
Val83′ Ser85′
Asp87′
Arg54′
Thr89′
Lys58′
A(–7)
A+1
G+3
3′5′
a
C(–10)
Figure 2 RNA-protein interface. (a) Schematic representation of PP7DFGinteractions with the RNA hairpin. Black arrows, hydrogen bonds; blacklines, van der Waals and stacking interactions. (b,c) The A(–7) (b) andA+1 (c) recognition pockets are shown, with the adenosine nucleotides(orange) and PP7DFG residues (blue, green) as sticks. Potential hydrogenbonds are shown as dashed lines.
BR I E F COMMUNICAT IONS
104 VOLUME 15 NUMBER 1 JANUARY 2008 NATURE STRUCTURAL & MOLECULAR BIOLOGY
A• e• e
S R 2m
a A l
AM N
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1
2
5 5 5 5
0AD AD AD AD
AD W D&
R AD
N
AD
A E
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1
4ii
PPR motif (35 aa)v t Y n t l I s g l c k a G r l e e A l e l f e e M k e k - G i a P d v1 4 ii
- A - G - U - C - A - C - U - G - A - G - A -
PPR protein
RNA
Base
Human mitochondrial RNA pol.Ringel et al., Nature (2011)
FTN VNS VTD FPD(1,4,ii)
Rec. base
[Base recognition code]
�Design and use of artificial RNA binding protein[PCT/JP2012/077274� patented in Japan, US.]
�Design and use of artificial DNA binding protein[PCT/JP2014/061329� patented in Japan, US]
• D,,
• 3 ( * ( C
• ,, d/ 11 A 4
• ,, Na C 3 niD ( * ( URP 5
4
( )
PPR technologyPentatricopeptide repeat
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• ( a s AN9• s l ds o 9• a i ~ cn g N9
What is the PPR proteinHuman (7)Yeast (2)
Prokaryote (0)Algae (0)
ArchaeaBacteria �0�
Plant �500�
[��PPR����������� ]
• P ( a ( b• m b %• ( a ( Ra 0 )
) ) b N AN9
M N s (• ( 0• ( ) �� �������• r e(• r e u �����������
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How to use PPR technology
PPR repeat
Sequence-specific binding module
Effector module Application
DNase
RNase
Splicing factor
Genome editing
Knock-down
Splicing controlwo/ module RNA remodeling
Block XX.(ex. miRNA, RBP)
AAV Nanoparticle
Translation factor Translation up
dPPR tech.(DNA-binding)
rPPR tech.(DNA-binding)
Plasmid
![Page 15: PPR technology...How to use PPR technology PPR repeat Sequence-specific binding module Effector module Application DNase RNase Splicing factor Genome editing Knock-down Splicing control](https://reader030.vdocuments.us/reader030/viewer/2022040112/5e9047d46d5b7f31b66f9f3f/html5/thumbnails/15.jpg)
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Nu
ER
DNA
mRNA
Protein
ncRNA
???
Pre-mRNA
mRNA
lncRNA
miRNA
RNA virus
RNA virus
mitochondria
Mitochondrial RNA
PPR
Possible rPPR-based applications
![Page 16: PPR technology...How to use PPR technology PPR repeat Sequence-specific binding module Effector module Application DNase RNase Splicing factor Genome editing Knock-down Splicing control](https://reader030.vdocuments.us/reader030/viewer/2022040112/5e9047d46d5b7f31b66f9f3f/html5/thumbnails/16.jpg)
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) 26DJO S0
(- 2 R EP NTS A
0 1
���������� ��
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A
D
DR
A N
AA N
DR=
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Beyond genome editing
mRNAtRNA
rRNA
ncRNAcircRNA
pre-mRNA
dsRNA
D
AE FCA D
A
)(
295
(
IR LN
)
)
)
D
)
PPR
PPR
!
( ))
)
PS
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)
19 8 0
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� ���� �����������
��� ������� ��������� ��
Thank you for your attention
Contact: www.editforce.jp(Keyword: editforce)