P15080: Flow Culture SystemMulti-Disciplinary Senior Design
Problem Definition Review
September 11, 2014Collin Burkhart – Lead Mechanical Engineer
Katelyn Busse – Lead Biomedical EngineeringMichelle Garofalo – Project Manager
Robert Repetti – Lead EDGE FacilitatorMorgan Stoessel - Treasurer
Sarah Tran – Note Taker
Agenda
● Project Background● Project Statement● Stakeholders● Use Scenarios ● Customer Needs and
Constraints● Engineering
Requirements
● House of Quality● Benchmarking ● Top Risks● Deliverables ● Project Plan● Questions
Project Background
● Cell cultureo What is it?
● Motivation for this project
Problem Statement
● Current Statuso 2D - Statico 3D - Static
● Current Limitationso Static Conditionso No current deviceo Micro vs. Macrofluidics
● The Main Objective
Stakeholders ● Dr. Jennifer Bailey● Biomedical engineering students taking
Advanced Cell Culture Class● Dr. Dan Phillips● Biomedical Engineering Department● Rochester Institute of Technology● MSD Team (and potential future MSD Teams)
Communication Plan● Follow up meeting with Dr. Bailey every two
weeks● E-mail customer with questions and
concerns at any time● Discuss budget with Dr. Phillips● Reach out to Dr. Man and Dr. Gaborski for
endothelial expertise
Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells
RIT
Instructor
Student(s)
Advanced Cell Culture class
offered
Retrieves flow culture system from storage
Inspects components for damage
Has students perform
experiment
Assembles components
of system
Sterilizes empty system in autoclave
Transport system to
biosafety hood
Replaces damaged
components
Damaged?
Yes
No
Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells
RIT
Instructor
Student(s)Add sterile
media to media reservoir
Seed growth plate
Add seeded growth plate to
growth chamber
Transfer to incubator
Power pump and sensory equipment
Activate pump to initiate
media flow
Ensure flow is continuous and free of bubbles
Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells
RIT
Instructor
Student(s)Adjust pump flow rate for
desired shear stress
Adjust incubator for desired pH
value
Adjust incubator temperature as
desired
Allow cells to grow
Cycle through new media
every 3-4 days
Disconnect growth chamber
to view cells
Reconnect growth chamber
after viewing
Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells
RIT
Instructor
Student(s) End experiment after two weeks
Deactivate pump
Cut power to pump and
sensory equipment
Transfer system to biosafety
hood
Disconnect growth
chamber
Drain remaining media from
system
Observe cells in growth chamber on microscopes
Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells
RIT
Instructor
Student(s)
Advanced Cell Culture class
ends
Students have completed experiment
Drain growth chamber
Autoclave all equipment
Return equipment to
instructor
Returns equipment to
storage
Process repeats for additional experiments
Customer Requirements
Importance: 1=Essential, 2=Customer Want, 3=Preference
Essential Customer Requirements
Sterility, Temperature, pH
Constraints
● It is assumed that the flow culture system will be using only animal endothelial cell lines
● This system may need to sit in an incubator to control temperature, media pH, and relative humidity
● Chamber that holds cells needs to fit on defined microscope stages
● System must be sterilized by autoclave● Budget of ~$1550
Engineering Requirements
Importance: 1=Essential, 2=Customer Want, 3=Preference
Essential Engineering Requirements
Essential Engineering RequirementsDescription and Tests
House of Quality
● Every customer requirement has been satisfied by at least one engineering requirement.
● Diagram can be found at the following location on EDGE:http://edge.rit.edu/edge/P15080/public/Problem%20Definition%20Documents/Additional%20Documents P15080_House_of_Quality document
Benchmarking● Perform cell culture in BME Lab with Dr.
Bailey● Research and contact representatives of
current companies that market cell culture flow devices
● Confer with Dr. Gaborski and Dr. Man
Top RisksBudget: ● Materials too expensive
Schedule: ● Team member delays● Unexpected labor hours
Technical:● Unable to keep materials sterile● Material malfunction● Pump inadequacies● Lack of knowledge in electrical engineering spectrum for sensor
configuration
Project Plan
Deliverables for Next Cycle
● Functional Decomposition 9/18● Concept Generation 9/23● Morph Table 9/23● Function/Engineering Analysis 9/23● Feasibility Analysis 9/25● System Design Review 9/30 or 10/2
Questions,Comments,
orConcerns?
Thank you!