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PROTEINSSBI4U
Ms. Manning
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Most diverse molecules in living organisms.
Coded for by genes in the DNA Used as:
Structural building blocksCatalysis - enzymes ImmunityTransport of other molecules across the cell
membrane Monomer:
Amino acid
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FOUR OF THE 7 CLASSES OF PROTEINS
Structural
Contractile
Storage
Transport
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20 AMINO ACID MONOMERS
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STRUCTURE OF AMINO ACIDS
Amino group –NH2
Carboxyl group -COOH
Hydrogen -H
Side group -R
Amino
group
Carboxylgroup
R group
Side groups
Leucine -hydrophobic
Serine-hydrophillic
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Amino acids have a central carbon with 4 things bonded to it:
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LINKING AMINO ACIDSCells link amino acids together to make proteins
The process is called condensation or dehydrationPeptide bonds form to hold the amino acids together
Carboxyl
Amino Side
Group
Dehydration Synthesis
Peptide Bond
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PROTEIN STRUCTURE
1 A.A. = 1 A.A. monomer 2 A.A.’s = a dipeptide molecule Many A.A.’s = a polypeptide chain 1 or more polypeptide chains = a protein
molecule.
There are 4 levels of protein structure: 1. Primary Structure 2. Secondary Structure 3. Tertiary Structure 4. Quaternary Structure
http://www.youtube.com/watch?v=lijQ3a8yUYQ&feature=related
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LEVELS OF STRUCTURE Primary A polypeptide chain
of A.A’s.
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SECONDARY STRUCTURE
A.A. chain will pleat or coil or form a helix.
H bonds!
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SECONDARY STRUCTURE Alpha helix Flexibility Polypeptide chain
tightly coiled held by H bonds
i.e. Keratin
http://www.youtube.com/watch?v=eUS6CEn4GSA&feature=related
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SECONDARY STRUCTURE Beta pleated sheet Strength Parallel polypeptide
chains H-bonds i.e. silk proteins
http://www.youtube.com/watch?v=wM2LWCTWlrE&feature=related
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TERTIARY STRUCTURE
Folded to form a specific shape (enzymes)
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3. TERTIARY – 3D folding (more complex) of polypeptide held in place by:
Hydrophobic bonding of non-polar side chains
Ionic bonds between charged side chains
Covalent disulfide bridges of cysteines
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FIBROUS VS. GLOBULAR Fibrous proteins are
strong and insoluble in water
eg. collagen, silk, keratin (main component in hair)
Globular proteins are spherical in shape,
Have a hydrophobic group on the inside, hydrophilic group on the outside.
Soluble in water. eg. enzymes, antibodies,
hormones
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Protein consists of one or more polypeptides twisted and coiled into a specific shape Shape is determined by the ORDER of amino
acids
Many proteins are globular (rounded shape), especially enzymes.
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4. QUARTERNARY – Two or more polypeptides wound together
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HEMOGLOBIN
C3032H4816O872N780S8Fe4
Build it!!!
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PROTEIN SHAPE ALSO INFLUENCED BY... Chemical and physical environmental factors
i.e. pH and temperature changes in environmental conditions may
cause changes in protein shape – called denaturation, protein becomes non-functional
Protein can resume shape under normal conditions if primary structure is maintained.
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DENATURATING PROTEINSChanges in temperature & pH can denature (unfold) a protein so it
no longer worksCooking denatures protein in eggs
Milk protein separates into curds & whey when it denatures
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CHANGING AMINO ACID SEQUENCESubstitution of one amino acid for
another in hemoglobin causes sickle-cell disease
(a) Normal red blood cell Normal hemoglobin
12 3
4 56
7. . . 146
(b) Sickled red blood cell Sickle-cell hemoglobin
2 314 5
67. . . 146
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OTHER IMPORTANT PROTEINS
• Blood sugar level is controlled by a protein called insulin
• Insulin causes the liver to uptake and store excess sugar as Glycogen
• The cell membrane also contains proteins
• Receptor proteins help cells recognize other cells
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INSULIN
Cell membrane with proteins & phospholipids
Copyrig
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