Download - Myxobacteria movement Cell Tracking Tanya Kazakova University of Notre Dame Advisor: Dr. Mark Alber
Myxobacteria movementCell Tracking
Tanya KazakovaUniversity of Notre DameAdvisor: Dr. Mark Alber
About Myxobacteria
The myxobacteria are Gram-negative organisms (0.5 x 5-7 mm rods) that are capable of multicellular, social behavior.
In the presence of nutrients, swarms of myxobacteria feed cooperatively. If the food supply is limited, the myxobacteria form aggregates called “fruiting bodies.”
Developmental Cycle for Myxobacteria
COUPLING CELL MOVEMENT TO MULTICELLULAR DEVELOPMENT IN MYXOBACTERIA by Dale Kaiser Nature Reviews Microbiology 1, 45-54 (2003)
Two engines of motility: AA and SS
A+S+
A+S-A-S+
AAdventurous SSocial
Cells move as a groupCells move alone
A-S- cells are unable to swarm or form fruiting bodies.
Two engines of motility (cont)
S-motilityThe pilus extends ahead of a cell, adheres to the fibrils on the cells ahead of it and then retracts, pulling the leading end of the piliated cell forward.
A-motilityCell is pushed by slime secretion.
At any instant, only one end secretes slime and only one end assembles pili.One end cannot do both at the same time.
Scenario
Suppose we have an image produced to exhibit the results of a biology experiment. In most cases, we will have a black and white drawing such as one exhibited on the left.
Goals
General: Get the most information from the
image
Specific:– Track individual cells– Make cell density more obvious– Show the boundaries of expanding front– Get rid of noise
About Scion Image
National Institutes of Health Package
http://rsb.info.nih.gov/nih-image/
Using SI: Options -> Color Tables
8 Grays 8 Grays->Fire 1
Original Image
Fire 1
Example
Cell Tracking Features
MATLAB
Example
Possible Directions for Future Work