Download - Myxobacteria movement Cell Tracking Tanya Kazakova University of Notre Dame Advisor: Dr. Mark Alber
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Myxobacteria movementCell Tracking
Tanya KazakovaUniversity of Notre DameAdvisor: Dr. Mark Alber
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About Myxobacteria
The myxobacteria are Gram-negative organisms (0.5 x 5-7 mm rods) that are capable of multicellular, social behavior.
In the presence of nutrients, swarms of myxobacteria feed cooperatively. If the food supply is limited, the myxobacteria form aggregates called “fruiting bodies.”
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Developmental Cycle for Myxobacteria
COUPLING CELL MOVEMENT TO MULTICELLULAR DEVELOPMENT IN MYXOBACTERIA by Dale Kaiser Nature Reviews Microbiology 1, 45-54 (2003)
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Two engines of motility: AA and SS
A+S+
A+S-A-S+
AAdventurous SSocial
Cells move as a groupCells move alone
A-S- cells are unable to swarm or form fruiting bodies.
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Two engines of motility (cont)
S-motilityThe pilus extends ahead of a cell, adheres to the fibrils on the cells ahead of it and then retracts, pulling the leading end of the piliated cell forward.
A-motilityCell is pushed by slime secretion.
At any instant, only one end secretes slime and only one end assembles pili.One end cannot do both at the same time.
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Scenario
Suppose we have an image produced to exhibit the results of a biology experiment. In most cases, we will have a black and white drawing such as one exhibited on the left.
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Goals
General: Get the most information from the
image
Specific:– Track individual cells– Make cell density more obvious– Show the boundaries of expanding front– Get rid of noise
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About Scion Image
National Institutes of Health Package
http://rsb.info.nih.gov/nih-image/
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Using SI: Options -> Color Tables
8 Grays 8 Grays->Fire 1
Original Image
Fire 1
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Example
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Cell Tracking Features
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MATLAB
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Example
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Possible Directions for Future Work