Is this specific for zebrafish?
Or can we learn how to drive stem cells into specific cell types
in mammals?
Overexpression of mouse Pou5f1 rescues zebrafish embryos mutant for Pou5f1
Evolutionary conservation of function
Overexpression of mouse Pou5f1 rescues zebrafish embryos mutant for Pou5f1
Evolutionary conservation of function
Mouse and zebrafish Pou5f1 share targets:
Comparison with mouse Oct4 target sets(genes with homology info only):Sharov et al, BMC Genomics 2008(Matoba et al., 2006 and Loh et al., 2006)
453 49 406
zebrafish mouse
Relevance?
Insight into controlled differentiation of stem cells to replace lost tissues:
Regenerative medicine
Use knowledge of combination of tissue specific repressors of
differentiation to generate defined cell types
Acknowledgements
& Rebecca Mössner, Kay Kotkamp, Bozena Polock, Björn Wendik, Esther Kur, Bernadette Lippok, Sungmin Song, Heinz-Georg-Belting, Martina Lachnit
Collaborations: Thorsten Kurz (ZBSA Genomics Core)
Daria Onichtchouk Florian Geier Jens Timmer (FRIAS)
Onichtchouk et al., Molecular Systems Biology, 6:354 Mar 9 - 2010
