Some Microscope Parts(lens to ‘see’ through;Magnifies 10x)
(Carry microscope)
(Carry microscope)
(lenses close to object/specimen;Magnifies 10x, 20x, or 40x)
NOSE PIECE(select objective lens)
STAGE (holds slide)
(Lifts or lowers stage to produce a focused image) (focuses/condenses
light on specimen)
(a.k.a body tube/keeps eyepiece at correct distance from objective)
(sharpens focus)
Iris Diaphragm (open 1/close 5) • Can be used to increase contrast (close 5)
• Or increase amount of light (open 1)
Diaphragm
Stage Clips
Diaphragmlever
Magnifying Power
Eyepiece x objective lens = TOTAL magnification
– LOW POWER: 10x X 10x = 100x
– HIGH POWER: 10x X 43x = 430x
• correct units for a microscope = micrometer (µm)
• 1 µm = (1/1000) mm
KEEP IT CLEAN!
• When to use…
Objective lens
Glass slides
Cover slips
Condenser Eye piece
Lens tissue
Slide tissue
X
X X
X X
Sequence for Viewing
1. plug in & turn on
2. find object in LOW POWER
3. focus with coarse adjustment & adjust light (light: 1 lets in the most & 5 least)
4. center object (still in low power)
5. turn to high power objective without changing focus
6. re-adjust light
7. sharpen focus with fine adjustment
PARFOCAL
• ability for the microscope to remain in focus when objective lens is changed
Low powerHigh Power
Store your Microscope Properly
1. Take slide OFF
2. Put lower power above stage
3. Roll nosepiece up (old); Stage down (new)
4. Turn OFF light
5. Wrap cord around scope
How not to break the microscope
• Always carry the microscope with 2 hands, one hand under the base & the other grasping the arm.
It _____________________
It turns it _______________
& _____________________
What a microscope does to an image…
Magnifies
Upside down
Backwards
As the power of the microscope
• Magnification increases
– The field of vision – The depth of field – Amount of light passing through the lens
system