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Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside
by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise F. Morgan, and Jay R. Lieberman
J Bone Joint Surg AmVolume 91(5):1073-1083
May 1, 2009
©2009 by The Journal of Bone and Joint Surgery, Inc.
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CFU-Fs were counted to estimate the mesenchymal stem cell concentrations.
Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083
©2009 by The Journal of Bone and Joint Surgery, Inc.
![Page 3: Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise](https://reader036.vdocuments.us/reader036/viewer/2022083007/56649e4f5503460f94b46ddd/html5/thumbnails/3.jpg)
The nucleated cell count of the bone marrow aspirate (BMA) was closely correlated to the mesenchymal stem cell (MSC) concentration of the bone marrow aspirate.
Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083
©2009 by The Journal of Bone and Joint Surgery, Inc.
![Page 4: Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise](https://reader036.vdocuments.us/reader036/viewer/2022083007/56649e4f5503460f94b46ddd/html5/thumbnails/4.jpg)
Radiographs of femoral defects in Groups I through VI at twelve weeks.
Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083
©2009 by The Journal of Bone and Joint Surgery, Inc.
![Page 5: Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise](https://reader036.vdocuments.us/reader036/viewer/2022083007/56649e4f5503460f94b46ddd/html5/thumbnails/5.jpg)
Radiographic scores were determined by the percentage of bridging bone at the defect site (1 = 0% to 25%, 2 = 26% to 50%, 3 = 51% to 75%, 4 = 76% to 99% or unicortical bridging of the defect,
and 5 = 100% or bicortical bridging of the defect).
Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083
©2009 by The Journal of Bone and Joint Surgery, Inc.
![Page 6: Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise](https://reader036.vdocuments.us/reader036/viewer/2022083007/56649e4f5503460f94b46ddd/html5/thumbnails/6.jpg)
Micro-computed tomography scans showing the bone formation within the femoral defects in Groups III, IV, and V at twelve weeks.
Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083
©2009 by The Journal of Bone and Joint Surgery, Inc.
![Page 7: Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise](https://reader036.vdocuments.us/reader036/viewer/2022083007/56649e4f5503460f94b46ddd/html5/thumbnails/7.jpg)
Histologic cross sections at the midpoint of the femoral defect.
Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083
©2009 by The Journal of Bone and Joint Surgery, Inc.