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Lycopene- Importance and Determination
through Double Beam UV-Vis Spectroscopy
Er. Aman PaulEr. Dorcus Masih
Sachin VermaEr. Sajan PalanchokeEr. Priyanka Malik
Dr. Justin Masih
National Conference
on “Chemistry:
Role and Challenges”
Department of
Chemistry,Ewing
Christian College
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"Until man duplicates a blade of grass, nature can laugh at his so-called scientific knowledge. Remedies from chemicals will never stand in favour compared with the products of nature, the living cell of the plant, the final result of the rays of the sun, the mother of all life.”
- Thomas Edison
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Introduction
• Lycopene is a bioactive carotenoid found in many red fruits and vegetables, such as tomatoes, watermelon, pink grapefruit, apricots, and pink guava.
• This natural red pigment is synthesized exclusively by plants and microorganisms.
• Structurally, lycopene is an acyclic polyene.
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Lyc
open
e co
nte
nt
of s
ome
fru
its
and
veg
etab
les
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Introduction (Continued)
• Lycopene from natural plant sources mainly exists in the all trans configuration, which is the most thermodynamically stable form.
• Heat processing of lycopene-containing plant foods induces “cis” isomerisation of lycopene, which has a beneficial effect on the absorption of lycopene.
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Tomato products
Raw Tomato
Lyc
open
e B
ioav
aila
bil
ity
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Dietary Significance
Carotenoids act by three main mechanisms to prevent oxidative damage in the body:
Electron Transfer
Hydrogen Abstraction
Addition of a radical species
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Dietary Significance (Continued)
Lycopene
Antioxidants
Pro-oxidants ASense the presence
of scavenger
Converts into leuco
form (antioxidant)
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Dietary Significance (Continued)
Functions of lycopene
Gap Junction Communication
Hypocholesteromic Anti-cancerous Anti-atherogenic
Oxidized LDL
Highly Carcinogenic
Lycopene converts it in unoxidized
state
Wei
sbur
ger
(200
2) h
as s
tate
d th
at th
ere
is a
low
er in
cide
nce
of c
hron
ic
dise
ases
in p
opul
atio
ns w
ith
regu
lar
inta
kes
of to
mat
o pr
oduc
ts th
atar
e ri
ch in
lyco
pene
.
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Estimation of Lycopene
1. Lycopene estimation is done AOAC method (AOAC, 1992).
2. Chemicals and Reagents- Acetone, Petroleum ether and Sodium sulphate.
3. Equipments- Systronics UV-VIS double beam spectrophotometer 2201
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5-10 gm sample repeated extracted with acetone until becomes colorless
Acetone extract is transferred to a separating funnel containing 10 to 15 ml petroleum ether
5% sodium sulphate solution is added
Lower acetone phase is repeatedly extracted with petroleum , until becomes colourless
Petroleum ether extract is pooled and its volume is made up to 50 ml with petroleum ether
Diluted an aliquot to 50 ml with petroleum ether
Colour is measured in a 1 cm cell at 503 nm in spectrophotometer
Methodology
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Calculation
Where,A = absorbance at 503 nm; D = dilution of extract to 100 ml;W = weight of sample taken.
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Conclusion
• Lycopene is a bioactive carotenoid found in many red fruits and vegetables.
• Structurally, lycopene is an acyclic polyene.• Heat processing of lycopene-containing plant
foods induces “cis” isomerisation of lycopene, which has a beneficial effect on the absorption of lycopene.
• It is an antioxidatant that serves as a magic ingredient in preventing many diseases.
• Lycopene estimation is done by using AOAC method (AOAC, 1992).
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