Living Organisms Consist of Cells

•State the resolution and magnification that can be achieved by a light microscope•Explain the difference between magnification and resolution•Explain the need for staining samples in light microscopy

Resolution and Magnification

Car Headlights from far away Car Headlights Close-Up

Define the Following Terms

• Magnification• Resolution

Light Microscope

• Number of lenses used to produce an image

• Light passes from the bulb (or mirror) under the stage through a condenser lens and then through the specimen

• The light beam is focused through the objective lens then the eyepiece lens

• How do you calculate the magnification power of a light microscope?• Eyepiece lens magnification x Objective lens magnification

• The max magnification of a light microscope is x1500

• The max resolution of a light microscope is 200nm

An Electron Microscope

Chloroplasts Electron micrograph

Leaf cells in moss ; viewed using light Microscope; chloroplasts visible, but not detailed

Optical microscope image of blood cell smear.

SEM image of red blood cells.

TEM image of a thin section of a blood vessel. A white blood cell is entering the tissue between two epithelial cells.