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Blood Supply
Lab 19, Slide 26
•75% of blood supply from gut (Venous Portal system)
•25% from Hepatic Artery
•Blood flows from the Portal Triad to the Central Vein via Sinusoids
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The Liver – A 3-D View
Lab 19, slide 25 http://www.biologymad.com/Kidneys/liverlobule.jpg
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Portal Triad (+lymph!)
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Portal Triad (+lymph!)
Lab 19, Slide 28
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Cords (or Plates) and Sinusoids
Plates of Hepatocytes
Blood-filled Sinusoids
Space of DisseLab 19, Slide 30
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Space of Disse
Microvilli
Endocytic Vacuole
•The Space of Disse is continuous with the lumen of the sinusoids due to spaces between the endothelial cells as well as in the basal lamina.
•This holds back cells, but not proteins. Proteins made in the liver can easily move into the blood.
Endothelium
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Central Vein
Lab 19, Slide 29
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Lymph
• Plasma that remains in the perisinusoidal space drains via lymphatics in the
OPPOSITE direction as blood – i.e. from hepatocytes to the triad, and finally to the
hilum.• 80% of the hepatic lymph drains into the
thoracic duct.
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Three LobulesClassic Lobule
Portal Lobule
Liver Acinus
SHAPE Polygon (hexagon)
Triangle Ellipse (or diamond)
CENTRAL VEIN
Central 3 Tips 2 Tips (triads at
other tips)
Portal triads at corners
Follows bile
drainage
Reflects blood supply
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Classic Lobule
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Portal Lobule
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Liver acinus
1 22 33
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Bile•Made by hepatocytes – it is secreted into canaliculi between cells
•Hering canals connect canaliculi to bile ducts
•Moves in opposite direction from blood – towards the portal triad
•Nasty stuff – Canaliculi are bounded by tight junctions
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Bile Canaliculi – Low Power
Phosphotungstic Acid & Hematoxylin stains the canaliculi.
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Bile Canaliculi
Canaliculus in Cross-section
Space of Disse
Tight Junctions
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Kupffer Cells•Macrophages, derived from monocytes.
•They form part of the lining of the sinusoid, but do not form junctions with endothelial cells.
http://medocs.ucdavis.edu/cha/402/PIX/1877/1877090.jpg
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What is at the pointer?
Is it carrying blood/bile to/from hepatocytes?
Portal Vein
It is carrying blood to the hepatocytes.
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Where is the highest O2 Content?
http://www.md.tsukuba.ac.jp/public/basic-med/anatomy/okado-group/Anatomy/C_description/c-1&2_liver/lobule.jpg
A B C
C – Zone 1 of the Acinus