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﷽
Lab. 3: Media Preparation.
Department of Botany
and Microbiology
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Primary ingredients required by all livingorganisms include:
a carbon source, water, minerals, and
a nitrogen source.
These nutrients together make a media.
Different microbes need different amounts ofthese nutrients.
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Broth (liquid).Semi-solid.Solid.
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Aim
To prepare the solid and broth media which contain the water and nutrients required for the growth of both bacteria and fungi.
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Material
1- Different Media:• Nutrient Agar (NA).• Nutrient Broth (NB).• Potato Dextrose Agar
(PDA).
2- Glassware: (Test tubes, flasks).
3-Disposable petri plates.
4- Scale.
5- Distilled water
6- Bunsen burners
7- Autoclave
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1 • Weigh
2 • Dissolve
3 • Sterilize
4
5
.Pour
Procedure
• Cool until use
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How could you prepare those types of Media?
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A-Pouring of Solid Media: In petri plates:
1. Write date and time on the bottom of plates.
2. Remove the lid slightly.3. Pour the media near
Bunsen burner.4. When solidify, invert
the plate and store at 4⁰C.
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B- Pouring of Solid Media: In tubes:
For Agar media: Slant Agar and / or Deep (Stab) Agar: Write labels on tubes After dissolving, pour media in test
tubes . Autoclave with loose caps. Place in slant or vertical position till
the media solidifies.
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Deep agarSlant agar
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2- Pouring of Solid Media: In tubes:
For broth media: Place the test tube near a burner
and remove the cap. Pour the media in the tube and close
the cap at once then autoclave. Place the tube in upright position in
the test tube stand.