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2 |Biochemistry laboratory
1. Aims of Biochemistry Experiments
Experiments Rules
Instruments Introduction
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GRADING
Theory EXAM : 70%Attendance(Theory): 10%Lab Performance: 20%
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2. Experiment Rules
① Put on your white lab coat before the class begin
Biochemistry laboratory
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2. Experiment Rules
② It is better done than said
Biochemistry laboratory
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2. Experiment Rules
③ Make Compensation
For Any Damage
Biochemistry laboratory
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2. Experiment Rules
④ Please shut off
the faucet and Power
Biochemistry laboratory
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2. Experiment Rules
⑤ Clean the classroom before you leave!
Biochemistry laboratory
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If you break any rules above, I will lower your
participation grade !
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3. Instruments Introduction
① check instruments for each group by the table below
category number category number
250-ml beaker 1 Dropper 1
100-ml beaker 1 Glass stirring rod 1
Large Test tube 18 Test-tube rack 1
Small Test tube 6 Bulb for pipette 1
Biochemistry laboratory
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3. Instruments Introduction
Beaker
Biochemistry laboratory
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3. Instruments Introduction
Test tube
Biochemistry laboratory
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Solution Mixing
Rotation
Fliping
Shaking
Biochemistry laboratory
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3. Instruments Introduction
Test-tube
rack
Biochemistry laboratory
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3. Instruments Introduction
Test-tube
brush
Biochemistry laboratory
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Test tube Washing
1. Flush the test tube with water At least 3 times
2. Scrub it with detergent both inside & outside
3. Flush the test tube with water at least 5 times just like step1
4. Rinse it with distilled water and place
them upside down on the rack to leave it dry
Biochemistry laboratory
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3. Instruments Introduction
Dropper
Biochemistry laboratory
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3. Instruments Introduction
Glass-stirring rod
Biochemistry laboratory
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3. Instruments Introduction
Tube clip
Biochemistry laboratory
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3. Instruments Introduction
Bulb for pipette
Biochemistry laboratory
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3. Instruments Introduction
Scaled pipette
Biochemistry laboratory
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Scaled Pipette Pattern:
0.5 ml 、 1 ml 、 2 ml
5 ml 、 10 ml etc.
Biochemistry laboratory
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• Hold Like This
Biochemistry laboratory
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Usage
Hold pipette
Absorb Solution
Adjust the Volume
Drop Solution
Biochemistry laboratory
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3. Instruments Introduction
Micropipette
Biochemistry laboratory
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Micropipette Specifications: 10 μl 、 20 μl 、 100 μl 、 200 μl 、 1000 μl & 5000 μl
Biochemistry laboratory
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THE INTRODUCTION ON PIPETTE
Biochemistry laboratory
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THE INTRODUCTION ON PIPETTE
Biochemistry laboratory
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Usage
1. Set the volume of solution
2. Choose paired tip ,and put on it
3. Absorb solution with 1st Lock , drop solution with 2nd Lock.
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Summary
1. Be careful
2. Be patient
3. Be lively
Biochemistry laboratory
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Please hand in your Report in time !
Thank youBiochemistry laboratory
Changsha Medical University
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LAB REPORT
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LAB REPORT
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LAB REPORT