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Intertwining of transposable elements
and non-coding RNAs in plant genomes
Douglas Silva Domingues
w/ Daniel Longhi Fernandes Pedro, Alexandre Rossi Paschoal
São Paulo State University, Institute of Biosciences at Rio Claro, Brazil
Federal Technology University of Paraná, Graduation Program in Bioinformatics,
Brazil
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Topics• ncRNAs – Non-coding RNAs
• TEs – Transposable Elements
• PlaNC-TE: a comprehensive knowledgebase of non-coding RNAs and transposable elements in plants
• Next steps
• Take home message
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What are ncRNAs?
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non-coding RNAs(ncRNAs)
Sequences that are not translated into protein
Wahlestedt, 2013
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Liu et al. 2017
ncRNAs Classification
Organized by:
LocationLength
FunctionStructure
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Central dogma of molecular biology.
DNA -> RNA -> Protein
Wahlestedt, 2013
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What are TEs?
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Major componentes in plant genomes and relevant to genome size!
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Transposable Elements – Classes
Own
Sequences that can change their position within a genome.
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WICKER et al. 2007
Class | Order | Superfamily
LTR: Long Terminal Repeatnon-LTR: non-Long Terminal Repeat
Transposable Elements – Hierarchy
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Why ncRNA:TEs?
Roberts et al. 2014 Adapted from: Maiti et al. 2012
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Why ncRNA:TEs?
Roberts et al. 2014 Adapted from: Maiti et al. 2012
Qin et al., 2015
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- ncRNA and TEs: known but ignored at in large-scale analyses
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Initial efforts
- First database to organize such information in plants
- PlanTE-MIR DB {10 ssp} – v.1 - 2016- miRNA:TE {152 evidences in 10 genomes}
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What if we expand this toall public plant genomes
with TE and ncRNAannotation data?
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http://planc-te.cp.utfpr.edu.br
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Objectives
- Extend PlanTE-MIR to all plant genomes available in Ensembl (53
species)
- Extend to all ncRNA classes available
- Make available a well-organized data- Lack of an organized repository of ncRNA:TEs for complete genomes in plants
- Standardize outputs
- Stimulate studies in TEs and ncRNAs in plant genomes
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PlaNC-TE - WorkflowA) SelectingncRNAs.
B) FilteringTEs.
C) ncRNA:TEanalysis
D) PlaNC-TEwebpages
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PlaNC-TE: A comprehensive knowledgebase of non-coding RNAs and transposable elements in plants.
- Genomic sequence source
- Ensembl Plants
- 53 genomes
- Retrieved sequences in ncRNAs and TEs
- ncRNAs – 58,390 records (53 genomes)
- TEs – 31,217,630 records (45 genomes)
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PlaNC-TE – Phylogenetic tree
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ncRNAs | TEs | % TE overlapping
https://doi.org/10.1093/database/bay078 - Figure 1
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Overall features
- Overlap features:- ~41% of the overlaps are among 4 genomes:
- Triticum aestivum; Zea mays; Oryza sativa; Arabidopsis thaliana
- Overlap records between ncRNA:TE | Public data available
- Visualization tools (Charts by genome and jBrowse)
- 14.350 overlaps in 40 genomes
- Scripts developed in Perl + Bash- Automatic updates
- ZendFramework2, Php7, MySQL, CSS3, HTML5, JavaScript and Debian9.
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PlaNC-TE – Detailed info
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- First page
- Select genome
- View ncRNA:TE overlaps
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From: PlaNC-TE: a comprehensive knowledgebase of non-coding RNAs and transposable elements in plantsDatabase (Oxford). 2018;2018. doi:10.1093/database/bay078
Database (Oxford) | The Author(s) 2018. Published by Oxford University Press.This is an Open Access article distributed under
the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted
reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
PlaNC-TE – Examples of nc:TEs
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PlaNC-TE – Download
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PlaNC-TE – Search & Browse
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PlaNC-TE – jBrowser
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http://planc-te.cp.utfpr.edu.br
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So...
- Extend PlanTE-MIR to all plant genomes available in Ensembl (53
species)
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So...
- Extend PlanTE-MIR to all plant genomes available in Ensembl (53
species)
- But TEs data are available only for 40 genomes!
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So...
- Extend PlanTE-MIR to all plant genomes available in Ensembl (53
species)
- But TEs data are available only for 40 genomes!
- If TEs are a major component of genomes, something is wrong!
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Phase 2: Re-annotationof TEs in complete plantgenomes
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Plant genomes Ensembl Plants Our approach
A. lyrata 116,145 391,425
A. thaliana 43,442 63,879
B. vulgaris 6,295 984,280
B. rapa 97,576 434,231
C. sativus - 176,333
M. acuminata 116,189 637,112
P. trichocarpa 248,622 864,831
V. vinifera 281,476 834,298
Initial analyses raised the numberof TE entries in genomes
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Take home message
• We still need standardization and better annotation(at least of TEs) in plant genomes
• Up to now, TE annotation is heavily based inalignment: curated datasets can be an startingpoint for other computational approaches
• Long-term goal: Are any specific characteristic(feature) of TE and/or ncRNA that distinguishncRNA:TE association?
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Team/Acknowledgements
Daniel L. F. Pedro
Alan P. Rodrigues Lorenzetti
Funding
Dr. Alexandre Rossi Paschoal
Tharcísio Amorim