Division Data SummaryResearch and Training Details
Number of Faculty 19
Number of Joint Appointment Faculty 2
Number of Research Fellows 3
Number of Research Students 2
Number of Support Personnel 90
Direct Annual Grant Support $7,837,314
Direct Annual Industry Support $3,178,485
Peer Reviewed Publications 48
Clinical Activities and Training
Number of Clinical Staff 5
Number of Clinical Fellows 3
Number of Clinical Students 1
Number of Other Students 12
Inpatient Encounters 2226
Outpatient Encounters 1290
Division Photo
Row 1: M Staat, R CardinRow 2: M Dickey, M Steinhoff, J Jiang, E
SchlaudeckerRow 3: N Hutchinson, M McNeal, S Shah, M TanRow 4: R Frenck, M Hostetter, D Bernstein, T
Farkas
Significant PublicationsMorrow AL, Meinzen-Derr J, Huang P, Schibler KR, Cahill T, Keddache M, Kallapur SG, Newburg DS, Tabangin
M, Warner BB, Jiang X. Fucosyltransferase 2 non-secretor and low secretor status predicts severe
outcomes in premature infants. J Pediatr. 158(5): p. 745-51 2011.
This landmark paper analyzed secretor gene fucosyltransferase 2 (FUT2) polymorphisms in relation to
outcomes of prematurity. Of the 410 study infants, death occurred in 13% of 95 infants who were
nonsecretors, 5% of 203 infants who were heterozygotes, and 2% of 96 infants who were secretors. Again by
phenotype, 15% of 135 infants with low secretor phenotype died, compared with only 2% of 248 infants with
high secretor phenotype. Low secretor phenotype was also associated with necrotizing enterocolitis and gram
negative sepsis. This paper provides the first definitive predictive biomarker of adverse outcomes in
premature infants.
Staat MA, Stadler LP, Donauer S, Trehan I, Rice M, Salisbury S. Serologic testing to verify the immune
status of internationally adopted children against vaccine preventable diseases. Vaccine. 28(50): p. 7947-
55. 2010.
Seven hundred forty-six children presenting to the CCHMC International Adoption Center between 11/1999
through 6/2004 were tested for serologic response to vaccine antigens received in their countries of origin.
More than 50% of the children were from Russia, Kazakhstan, or the Ukraine. Another 21% of the children
were from China, 12% from Guatemala, and the remaining children from South Korea, Eastern Europe, India,
Asian/Pacific Rim, Vietnam, or Africa. For children with 3 or more vaccine doses, overall protection was high
Infectious Diseases
for diphtheria (85%), tetanus (95%), polio (93%), and hepatitis B (77%). Children without immunization
documentation had lower immunity. Serologic testing is an important criterion to verify the immunization
status of internationally adopted children.
Bravo FJ, Bernstein DI, Beadle JR, Hostetler KY, Cardin RD. Oral hexadecyloxypropyl-cidofovir therapy in
pregnant guinea pigs improves outcome in the congenital model of cytomegalovirus infection. Antimicrob
Agents Chemother. 55(1): p. 35-41. 2011.
The authors evaluated the in vivo efficacy of an orally bioavailable analogue of cidofovir, hexadecyloxypropyl-
cidofovir (HDP-CDV), in a guinea pig model of congenital CMV infection. Pregnant guinea pigs were
inoculated with GP-CMV during the second/early third trimester of gestation, and the treatment groups
received one of two HD-CDV regimens. All HDP-CDV regimens significantly improved pup survival from 50-
60% in untreated animals to 93-100% in treated animals. Treatment with 20 mg/kg HDP-CDV significantly
reduced the viral load in pup spleen and liver, while treatment with 4 mg/kg did not. These results indicate
that HDP-CDV has promise for treatment of congenital human CMV infection, which can produce both
sensorineural hearing loss and mental retardation.
Farkas T, Cross RW, Hargitt E, Lerche NW 3rd, Morrow AL, Sestak K. Genetic diversity and histo-blood group
antigen interactions of rhesus enteric caliciviruses. J Virol. 84(17): p. 8617-25. 2010.
The group examined rhesus macaque stool samples for rhesus caliciviruses (CVs). Approximately 11% of
the samples contained recovirus isolates. Phylogenetic analysis classified the recovirus isolates into two
genetic groups and at least four genetic types. A single rhesus norovirus isolate was closely related to GII
human noroviruses. Neutralizing antibodies against the Tulane virus, a novel rhesus calicivirus, were detected
in 88% of serum samples obtained from primate caregivers. Type A and B histo-blood group antigens were
involved in Tulane virus infection. These findings indicate the zoonotic potential of primate CVs and bring to
light some remarkable similarities between rhesus enteric CVs and human noroviruses.
Tan MX, Jiang X. Norovirus gastroenteritis, carbohydrate receptors, and animal models. PLoS Pathog. 6(8).
2010.
This invited review in a prestigious journal highlighted the importance of histo-blood group antigens as
receptors for noroviruses. Different noroviruses reveal different receptor-binding profiles associated with the
ABO, secretor, and Lewis HBGA types. Human volunteer challenge studies of a prototype Norwalk virus
provided direct evidence of HBGA receptor recognition. The HBGA-binding interfaces have been identified in
the protruding (P) domain of the viral capsid protein, demonstrating that the P domain is the primary site of
receptor interaction, which plays an essential role in norovirus infection.
Division Highlights
Margaret K. Hostetter, MD
The Hostetter laboratory focuses on mechanisms and consequences of heparin binding by Candida albicans.
Binding of heparin by C. albicans has been shown by our laboratory to be essential for:
(1) removal of candidal surface proteins that serve as targets for elimination of the organism by innate
immunity
(2) exposure of a C. albicans superantigen, defined in J Inf Dis 2008; 197:981-9
(3) biofilm formation in vivo
Collaborators at the University of Cincinnati and CCHMC have helped us to define the mechanism of heparin
binding. With Jason Lu, CCHMC Bioinformatics, we evaluated linear heparin binding sites in 400 C. albicans
surface proteins. Fifty-six C. albicans proteins were found to have heparin-binding sites that conformed to the
Cardin, Weintraub, or Sobel motifs. Of these 56, 13 were expressed at the bud neck, the site at which the
yeast cell undergoes hyphal morphogenesis. These 13 proteins participated in various functions including
chitin synthesis, anti-fungal resistance, GTPase activation, and phosphorylation. The protein with the most
heparin binding motifs was Int1, which we had defined in our laboratory (Science 1998; 279:1355-8). In
collaboration with Alexey Porollo, UC Environmental Health, we identified a cup-like structural motif in at
least one of these sites that was conformationally consistent with heparin binding. A collaboration with Apryl
Stallcup, UC Chemistry, provided additional information regarding the structure of heparin and possible sites
of interaction with positively charged amino acids. Lastly, in a collaboration with Ken Greis, UC Proteomics
laboratory, we refined the method of SILAC to examine proteins removed from the C. albicans surface after
incubation with heparin. Of the top 25 proteins removed (of more than 100), 13 had identifiable heparin
binding sites. The proteomics project received core grant funding from the CCTST. These collaborative efforts
have provided key insights into the biochemistry of heparin binding.
David I. Bernstein, MD, MA
The pivotal trial for a genital herpes vaccine composed of herpes simplex type 2 glycoprotein D (gD) and
adjuvanted with MPL and Alum was completed. Although two large trials showed efficacy in women this trial
showed no efficacy for prevention of genital herpes caused by HSV type 2 (HSV-2) but surprisingly
demonstrated efficacy against HSV-1 genital herpes. On a more positive note, our preclinical evaluations of
vaccines that also contained gB and gH/gL combined with cationic lipid DNA complexes as an adjuvant
showed protection, including protection of the dorsal route ganglia, the site of HSV latency.
The large multicenter cytomegalovirus trial that screens infants for congenital CMV has enrolled over 95,000
newborns. We have found that dried blood spots are not suitable for screening (published in JAMA) but that
saliva was an excellent source for this screen (published in the NEJM).
Turning to GI viruses we completed the first human trial of a vaccine for norovirus and found it to be
moderately protective (accepted for publication, NEJM) while the rotavirus vaccine we developed showed
continued efficacy in preventing hospitalizations and deaths around the world.
Rebecca Brady, MD
We published three manuscripts regarding human antibody and T cell responses elicited by influenza
vaccines against the hemagglutinin protein.
Rhonda Cardin, PhD
In previous studies, the Cardin lab showed that the viral chemokine receptor M33 encoded by murine
cytomegalovirus (CMV) is required for long term latent infection of the bone marrow. In 2010, we identified
several cell types in the bone marrow which are latently infected, and importantly, when the virus has a
mutation in the M33 gene, some of these cell types do not become latently infected. This is the first
identification of bone marrow cells which harbor latent CMV infection and the role that M33 plays during
latent CMV infection at the cellular level. In collaboration with Dr. Helen Farrell in Australia, we have shown
that the viral chemokine receptor US28 homolog encoded by human CMV rescues the latency defect of a
mutant M33 virus, thus suggesting that the human CMV US28 protein plays a similar role in the
establishment of CMV latency in humans. Also, in collaboration with Dr. David Bernstein and Dr. Daniel
Choo, we have shown that infection of guinea pigs with guinea pig CMV induces hearing loss as measured
by Auditory-Evoked Brainstem Analysis (ABR). We find that guinea pig CMV infects the cochleas of newborn
guinea pigs which are experimentally infected at 48 hours after birth (neonatal CMV model) or in newborn
guinea pigs which were infected in utero following infection of the pregnant guinea pig (congenital CMV
model). Both models show that ~50% of the infected pups develop hearing loss. Development of the guinea
pig CMV hearing loss models is significant since human CMV is the leading infectious cause of hearing loss,
with approximately 0.5-2.0% of newborn infants experiencing hearing loss within the first 2-4 years of life.
Studies are underway to determine the underlying mechanisms of CMV-induced hearing loss and to
determine if antiviral therapy can prevent the loss.
Tibor Farkas, DVM, PhD, MBA
The Farkas lab studies enteric viral diseases of humans and animals. In 2010 we continued our work on non-
human primates (NHP) enteric caliciviruses with the major goal to develop a new disease model for human
norovirus (NoV) gastroenteritis. Our work demonstrated remarkable similarities between rhesus enteric
caliciviruses (ReCV) and human NoVs including their epidemiology, genetic and antigenic diversity, putative
receptor (histo-blood group antigen; HBGA) binding and the ability to induce gastroenteritis in challenged
animals. Over15 ReCVs representing different serotypes and HBGA types were tissue culture adapted.
Current work is focusing on the development and evaluation of the animal, tissue culture and surrogate
model that duplicates the diversity of human NoVs.
In 2010 we described two novel picornavirus groups in turkeys and chickens, including birds with runting
stunting syndrome (RSS). RSS of chickens and poult enteritis mortality syndrome (PEMS) of turkeys are the
two most significant enteric diseases of poultry. The causative agent(s) of RSS and PEMS are yet
unidentified. Establishing the role of picornaviruses identified in our laboratory in enteric diseases of poultry is
ongoing.
Robert W. Frenck, MD
We completed the first ever human challenge model with a G2 strain of norovirus. Dr. Bernstein is the IND
holder for the virus and Dr. Jiang and Dr Frenck were Principal Investigators on the study. This study has
been parlayed into a project with LigoCyte to see if their G2 norovirus vaccine can protect people with
subsequent challenge with our G2 strain.
Jason Jiang, PhD
Noroviruses are an important cause of acute gastroenteritis. In the past year we have made significant
advancements in understanding the virus/host interaction and receptors for noroviruses. We have resolved
the crystal structures of the first non-secretor binding strain (VA207) which would greatly facilitate our future
study on the screening and design antivirals against noroviruses. Our preliminary study on silico screening for
antivirals against noroviruses based on these crystal structures is ongoing. We have completed the first
human volunteer challenge study on a GII.4 norovirus and demonstrated the association of GII.4 infection
with the histo-blood types of the volunteers, which concluded one of our projects funded by the DoD. This
challenge model is very valuable for future vaccine and antiviral evaluation because it is the first human
volunteer challenge with a GII.4 norovirus and the GII.4 noroviruses have been found predominant in many
countries around the world. In fact, following the DoD study, we have initiated another study funded by
LigoCyte to determine the 50% infectious dosage of the challenge pool. We anticipate a number of vaccine
trials will be initiated in the near future using this challenge model, including evaluation of our P particle
vaccine licensed to LigoCyte. Our study on the evolution of noroviruses has been summarized in a review
article published recently in Trend in Microbiology. Our study on the development of norovirus P particles as
a candidate vaccine against noroviruses is in the second year of a NIH R01 and we have made excellent
progress in the characterization of norovirus antigenic and HBGA receptor binding variations, the
establishment of animal model for vaccine efficacy evaluation and scale up production of P particles for
future animal and clinical evaluation. Finally, we have initiated a number of new studies in
inactivation/neutralization of Tulane virus replication by silence RNA, screening antivirals against TV protease,
diagnosis and passive immunization of norovirus infection with IgY developed in chickens, the study of viral
host receptors of rotaviruses, and establishment of TV as a surrogate for human noroviruses in food safety
research against norovirus contamination funded by the USDA.
Monica McNeal, MS
The Laboratory for Specialized Clinical Studies continues to supply lab support for a large number of clinical
studies involving vaccine trials and vaccine development. Influenza virus vaccines and rotavirus vaccines
continue to be important for overall health of children in the US and around the world. The lab is committed
to help establish clinical labs in India to support rotavirus vaccine trials in that country. In addition, the lab
consults with other labs around the world to provide training and support for establishing quality assays to
support vaccine trials.
Nancy Sawtell, PhD
Most of the human population world-wide has been infected by herpes simplex viruses. Following the initial
lytic infection, HSVs establish permanent latent infections within sensory neurons. Reactivation of latent virus
not only results in viral disease (new infections, blindness and encephalitis) but also contributes to HIV
infection, diabetes, cardiovascular and neurodegenerative diseases. No effective vaccine is available and no
therapy eliminates latency or prevents reactivation. The long-term goal of ongoing research in the Sawtell lab
is to find interventions for recurrent HSV episodes by defining mechanisms that control establishment and
reactivation of HSV-1 latency.
The gene expression cascade during HSV-1 lytic infection begins with activation of immediate-early (IE) gene
transcription by the virion protein VP16 with host factors Oct-1 and HCF-1. In contrast, the initial events in
the reactivation from latency are still poorly defined. Ourcentral hypothesis is that regulation of both VP16
expression and activity underlie the establishment of latency and reactivation from latency. These two levels
of control involve multiple positive and negative inputs to allow or inhibit viral replication in the sensory
neuron in vivo.
Division CollaborationBone Marrow Transplantation and Immune Deficiency » Stella M. Davies, MBBS, PhD, MRCP
Dr. Robert Frenck has established a collaboration with Bone Marrow Transplant to evaluate the
immunogencity of the 13-valent pneumococcal conjugate vaccine in patients who have undergone bone
marrow transplantation.
General and Community Pediatrics » Sheela Rath Geraghty, MD, MS, IBCLC, FAAP; Robert Schaengold , MD;
Mary Beth Pero, MD
Dr. Robert Frenck has established a collaboration with General Pediatrics to evaluate a new H. influenzae
vaccine in infants.
Immunobiology » Marsha Wills-Karp, PhD
Dr. Margaret Hostetter has established a collaboration with Immunobiology on the role of Candida albicans in
induction of asthma in murine models.
Perinatal Biology » Alan H. Jobe, MD, PhD
Dr. Margaret Hostetter has established a collaboration with Perinatal Biology on the effects of Candida
albicans in amniotic fluid of sheep.
Biomedical Informatics » Long (Jason) Lu, PhD; Jarek Meller, PhD
Dr. Margaret Hostetter has established a collaboration with Biomedial Informatics on informatics approach to
identify linear heparin binding motifs on C. albicans cell wall proteins with Dr. Lu.
Dr. Jason Jiang has worked with Dr. Meller on structural analysis of noroviruses in interaction with histo-
blood group antigen carbohydrates.
Molecular Genetics at the Univeristy of Cincinnati College of Medicine » Malak Kotb, PhD; Richard L.
Thompson, PhD
Dr. Nancy Sawtell has worked in collaboration with Drs. Kotb and Thompson to establish a forward genetic
approach to identifying host genes modifying herpes simplex virus pathogenic outcomes.
Dr. Nancy Sawtell has also worked in collaboration with Dr. Thompson on HSV latency and herpetic eye
disease and transcriptional and posttranscriptional regulation of VP16 in neurons and the control of HSV
latency, entry and exit.
Center for Epidemiology and Biostatistics » Ardythe L. Morrow, PhD
Dr. Jason Jiang has collaborated with Dr. Morrow in determining the host receptors for rotavirus based on
the approach utilized in the study of receptors of noroviruses.
Center for Acute Care Nephrology » Stuart L. Goldstein, MD
Dr. Rebecca Brady has worked with Dr. Goldstein on a quality improvement project to address the reduction
of nephrotoxic medication-associated acute kidney injury and associated costs in hospitalized children.
Experimental Hematology » Maria-Dominique Filippi, PhD
Dr. Rhonda Cardin has worked with Dr. Filippi on the identification of the latent CMV infected cells in the
bone marrow.
Otolargygnology » Daniel Choo, MD
Dr. Rhonda Cardin has worked with Dr. Choo in the analysis of hearing loss in the guinea pig CMV
models characterized by Drs. Bernstein and Cardin.
Molecular Immunology » Kasper Hoebe, PhD
Dr. Rhonda Cardin has worked on the collaboration to characterize murine CMV infection in various mutant
mice generated in Dr. Hoebe's lab and to determine the role of NK cell and innate immunity on latent CMV
infection.
Pediatric Surgery » Greg Tiao, MD
Monica McNeal, MS has collaborated with Dr. Tiao using rotavirus to induce a mouse model of biliary
atresia. Research involves looking at receptor differences, effects of different rotaviruses to induce the model
and cell signaling pathways.
Gastroenerology » Sean Moore, MD
Monica McNeal, MS has collaborated with Dr. Moore on establishing a malnutrion model in mice to use to
look at the effects on live rotavirus vaccines.
Human Genetics » Derek Neilson, MD
Monica McNeal, MS, has collaborated with Dr. Neilson on establishing an influenza model in mice to look at
a gene that is involved in acute necrotizing encephalopathy.
Molecular Genetics and Chemistry, UC » Alison Weiss, PhD ; Suri Saranathan Iyer, PhD
Monica McNeal, MS has collaborated with Drs. Weiss and Iyer on developing receptor mimics for rapid
dection, typing and susceptibility testing of influenza.
Faculty MembersMargaret K. Hostetter, MD, Professor
Director, Division of Infectious DiseasesAlbert Sabin Professor of PediatricsResearch Interests Candida albicans
David I. Bernstein, MD, MA, ProfessorDirector, Gamble Program for Clinical StudiesDirector, VTEUResearch Interests Vaccines, rotavirus, herpes simplex, cytomegalovirus
Steven Black, MD, Adjunct
Research Interests Vaccine safety
Rebecca C. Brady, MD, Associate ProfessorDirector of Adult Clinical StudiesResearch Interests Adult vaccines, influenza
Rhonda D. Cardin, PhD, Assistant Professor
Research Interests Cytomegalovirus, genital herpes vaccines
Beverly L. Connelly, MD, ProfessorDirector, Pediatric Infectious Diseases Fellowhship Training ProgramDirector, Infection Control ProgramResearch Interests Infection control, Healthcare quality improvement
Michelle P. Dickey, MS, CRN, InstructorManager, Gamble ProgramResearch Interests
Tibor Farkas, DVM, PhD, MBA, Assistant Professor
Research Interests Enteric viral diseases
Robert W. Frenck, MD, ProfessorChairman, Institutional Review BoardDirector of Clinical MedicineResearch Interests Vaccines
Michael A. Gerber, MD, ProfessorDirector, Clinical Care and TeachingMedical Director, Continuing Medical EducationResearch Interests
Nancy M. Hutchinson , RN, MSN, CIC, InstructorInfection Control ProgramResearch Interests
Xi Jason Jiang, PhD, Professor
Research Interests Caliciviruses. rotavirus, vaccines
Monica M. McNeal, MS, InstructorAssociate Director, LSCSResearch Interests
Nancy M. Sawtell, PhD, Associate Professor
Research Interests Herpes simplex virus
Mary A. Staat, MD, MPH, ProfessorDirector, International Adoption CenterResearch Interests Rotavirus, epidemiology, international adoption, vaccine preventable diseases
Mark C. Steinhoff, MD, ProfessorDirector, Global Health CenterResearch Interests Maternal immunization
Jane E. Strasser, PhD, AdjunctDirector, UC Office of Research Compliance and Regulatory AffairsResearch Interests Shiga like toxins, genetics of susceptibility and resistance
Ramu Subbramanian, PhD, Assistant Professor
Research Interests Influenza, T cell immunity
Ming Tan, PhD, Assistant Professor
Research Interests Calicivirus
Joint Appointment Faculty MembersSteve Black, MD, Adjunct
Center for Global Child Health
Mark C. Steinhoff, MD, Professor
Center for Global Child Health
Clinical Staff MembersTracy Byrne, RN, OPAT Clinic, International Adoption Center
Kelly Hicks, RN, MSN, International Adoption Center
Jennifer Kelley, APN, OPAT Clinic
Susan Ruedy, MA, International Adoption Clinic
Tisha Way, MSSA, LISW-S, International Adoption Clinic
TraineesJulianne Green, MD, PhD, PL-5, University of Louisville Collete of Medicine
Andrew Kreppel, MD, PL-5, St. Louis University School of Medicine
Elizabeth Schlaudecker, MD, PL-7, University of Cincinnati College of Medicine
Diana Koch, Graduate Student, PGY-3, Univeristy of Cincinnati College of Medicine
Ryan Walker, Graduate Student, PGY-1, Univeristy of Cincinnati College of Medicine
Significant Accomplishments
Diarrheal Viruses
Jason Jiang, PhD, and Ming Tan, PhD, have extended their work on the norovirus P particle and its
adaptability as a vaccine platform. The norovirus P particle is formed by 24 copies of the protruding (P)
domain of the norovirus capsid, which is ideally sized for a subunit vaccine. Their studies have shown that
insertion of the M2e epitope of influenza virus and the VP8 epitope of rotavirus into the three surface loops of
each P domain induced high titers of neutralizing antibodies against replication of influenza virus and
rotavirus and protected vaccinated mice from infection with these two viruses.
The Rotarix vaccine, invented by David Bernstein, MD, and Richard Ward, PhD, was recently found to be
associated with a 42 percent decrease in deaths per 100,000 children aged 11 months or younger in Mexico.
Diarrhea-related mortality was 29 percent lower for children between the ages of 12 and 23 months, although
these children are typically too old to receive the vaccine. Diarrhea-related mortality among unvaccinated
children between the ages 24 and 59 months was not significantly reduced. (Richardson V. et. al., New
England Journal of Medicine, 2010; 362:299-305).
Herpes Viruses
Nancy Sawtell, PhD, has continued her work on the herpes simplex virus type 1 (HSV-1) virion protein VP16
as a central mediator of latency and reactivation from latency in sensory neurons. Innovative animal models
allow testing of the hypothesis that the VP16 gene in the HSV-1 genome can be regulated by action of
neuro-specific and stress-responsive promoter elements and corresponding transcription factors to allow the
differential responses entailed in initial infection or exit from latency. Sawtell hopes to identify transcription
factors or protein-modifying enzymes that regulate VP16 and its differential functions as promising targets for
development of therapeutic interventions for HSV reactivation.
Rhonda Cardin, PhD, has developed a guinea pig model that accurately recapitulates transmission of
cytomegalovirus (CMV) from mother to fetus and is accompanied by attendant hearing loss. Since CMV
infection in humans is the major cause of nonhereditary deafness, this critically important animal model will
allow dissection of each step of pathogenesis and serve as an important tool for testing or therapeutic
interventions.
Expansion of Clinical Services
Effective Jan. 1, 2011, Robert Frenck, MD, has assumed the position of chief of the Clinical Service after the
departure of Michael Gerber, MD. Under Frenck’s leadership, divisional revenues from inpatient consults have
increased by 37 percent, and the outpatient antibiotic service has had an increase of more than 40 percent in
patient encounters over the past two years. Frenck’s plans call for the opening of a travel clinic at the
Cincinnati Children’s Burnet Campus and a general infectious disease clinic at Liberty Campus within the
next 12 months.
In continuing to refine their model of services for internationally adopted children, Mary Staat, MD, and the
International Adoption Center have added mental health services and family counseling to help internationally
adopted children overcome abusive backgrounds and to facilitate families’ adjustment to the emotional and
educational needs of internationally adopted children.
Division Publications1. Abdulla RY, Rice MA, Donauer S, Hicks KR, Poore D, Staat MA. Hepatitis A in internationally adopted
children: screening for acute and previous infections. Pediatrics. 2010; 126:e1039-44.
2. Allen SR, Fiorini P, Dickey M. A streamlined clinical advancement program improves RN participation
and retention. J Nurs Adm. 2010; 40:316-22.
3. Basha S, Hazenfeld S, Brady RC, Subbramanian RA. Comparison of antibody and T-cell responses
elicited by licensed inactivated- and live-attenuated influenza vaccines against H3N2 hemagglutinin.
Hum Immunol. 2011; 72:463-9.
4. Bernstein DI, Earwood JD, Bravo FJ, Cohen GH, Eisenberg RJ, Clark JR, Fairman J, Cardin RD. Effects of
herpes simplex virus type 2 glycoprotein vaccines and CLDC adjuvant on genital herpes infection in
the guinea pig. Vaccine. 2011; 29:2071-8.
5. Black S. Travelers' protection against meningococcal disease: a new vaccine option. J Travel Med.
2010; 17 Suppl:18-25.
6. Black S, Della Cioppa G, Malfroot A, Nacci P, Nicolay U, Pellegrini M, Sokal E, Vertruyen A. Safety of
MF59-adjuvanted versus non-adjuvanted influenza vaccines in children and adolescents: an
integrated analysis. Vaccine. 2010; 28:7331-6.
7. Boppana SB, Ross SA, Shimamura M, Palmer AL, Ahmed A, Michaels MG, Sanchez PJ, Bernstein DI,
Tolan RW, Jr., Novak Z, Chowdhury N, Britt WJ, Fowler KB. Saliva polymerase-chain-reaction assay for
cytomegalovirus screening in newborns. N Engl J Med. 2011; 364:2111-8.
8. Brady R. Zygomycosis (Mucormycosis). Rudolph's Pediatrics. New York: McGraw-Hill; 2011.
9. Brady RC. Influenza. Adolesc Med State Art Rev. 2010; 21:236-50, viii.
10. Bravo FJ, Bernstein DI, Beadle JR, Hostetler KY, Cardin RD. Oral hexadecyloxypropyl-cidofovir therapy
in pregnant guinea pigs improves outcome in the congenital model of cytomegalovirus infection.
Antimicrob Agents Chemother. 2011; 55:35-41.
11. Campbell JD, Chambers CV, Brady RC, Caldwell MC, Bennett NL, Fourneau MA, Jain VK, Innis BL.
Immunologic non-inferiority of a newly licensed inactivated trivalent influenza vaccine versus an
established vaccine: A randomized study in US adults. Hum Vaccin. 2011; 7:81-8.
12. El-Kamary SS, Pasetti MF, Mendelman PM, Frey SE, Bernstein DI, Treanor JJ, Ferreira J, Chen WH,
Sublett R, Richardson C, Bargatze RF, Sztein MB, Tacket CO. Adjuvanted intranasal Norwalk virus-like
particle vaccine elicits antibodies and antibody-secreting cells that express homing receptors for
mucosal and peripheral lymphoid tissues. J Infect Dis. 2010; 202:1649-58.
13. Fairbrother G, Cassedy A, Ortega-Sanchez IR, Szilagyi PG, Edwards KM, Molinari NA, Donauer S,
Henderson D, Ambrose S, Kent D, Poehling K, Weinberg GA, Griffin MR, Hall CB, Finelli L, Bridges C,
Staat MA. High costs of influenza: Direct medical costs of influenza disease in young children.
Vaccine. 2010; 28:4913-9.
14. Farkas T, Cross RW, Hargitt E, 3rd, Lerche NW, Morrow AL, Sestak K. Genetic diversity and histo-blood
group antigen interactions of rhesus enteric caliciviruses. J Virol. 2010; 84:8617-25.
15. Farrell HE, Abraham AM, Cardin RD, Sparre-Ulrich AH, Rosenkilde MM, Spiess K, Jensen TH, Kledal TN,
Davis-Poynter N. Partial Functional Complementation between Human and Mouse Cytomegalovirus
Chemokine Receptor Homologues. J Virol. 2011; 85:6091-5.
16. Fuller CA, Pellino CA, Flagler MJ, Strasser JE, Weiss AA. Shiga toxin subtypes display dramatic
differences in potency. Infect Immun. 2011; 79:1329-37.
17. Gerber MA, Brown HW, Lee G, Tanz RR, Temte JL, Van Beneden CA. Physicians' opinions about
critical attributes of a potential group A streptococcal vaccine. Vaccine. 2010; 28:7155-60.
18. Henkle E, Steinhoff MC, Omer SB, Roy E, Arifeen SE, Raqib R, McNeal M, Breiman RF, Moss WJ, Zaman
K. Incidence of influenza virus infection in early infancy: a prospective study in South Asia. Pediatr
Infect Dis J. 2011; 30:170-3.
19. Knipping K, McNeal MM, Crienen A, van Amerongen G, Garssen J, Van't Land B. A gastrointestinal
rotavirus infection mouse model for immune modulation studies. Virol J. 2011; 8:109.
20. Matson DO, Abdel-Messih IA, Schlett CD, Bok K, Wienkopff T, Wierzba TF, Sanders JW, Frenck RW, Jr..
Rotavirus genotypes among hospitalized children in Egypt, 2000-2002. J Infect Dis. 2010; 202
Suppl:S263-5.
21. McNeil SA, Dodds LA, Fell DB, Allen VM, Halperin BA, Steinhoff MC, MacDonald NE. Effect of respiratory
hospitalization during pregnancy on infant outcomes. Am J Obstet Gynecol. 2011; 204:S54-7.
22. Morrow AL, Meinzen-Derr J, Huang P, Schibler KR, Cahill T, Keddache M, Kallapur SG, Newburg DS,
Tabangin M, Warner BB, Jiang X. Fucosyltransferase 2 non-secretor and low secretor status predicts
severe outcomes in premature infants. J Pediatr. 2011; 158:745-51.
23. Omer SB, Goodman D, Steinhoff MC, Rochat R, Klugman KP, Stoll BJ, Ramakrishnan U. Maternal
influenza immunization and reduced likelihood of prematurity and small for gestational age births: a
retrospective cohort study. PLoS Med. 2011; 8:e1000441.
24. Poehling KA, Fairbrother G, Zhu Y, Donauer S, Ambrose S, Edwards KM, Staat MA, Prill MM, Finelli L,
Allred NJ, Bardenheier B, Szilagyi PG. Practice and child characteristics associated with influenza
vaccine uptake in young children. Pediatrics. 2010; 126:665-73.
25. Poehling KA, Szilagyi PG, Staat MA, Snively BM, Payne DC, Bridges CB, Chu SY, Light LS, Prill MM, Finelli
L, Griffin MR, Edwards KM. Impact of maternal immunization on influenza hospitalizations in infants.
Am J Obstet Gynecol. 2011; 204:S141-8.
26. Riddle MS, Rockabrand DM, Schlett C, Monteville MR, Frenck RW, Romine M, Ahmed SF, Sanders JW. A
prospective study of acute diarrhea in a cohort of United States military personnel on deployment to
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Grants, Contracts, and Industry Agreements
Grant and Contract Awards Annual Direct / Project Period Direct
BERNSTEIN, D
The Natural History of CMV-Related Hearing LossNational Institutes of Health(University of Alabama-Birmingham)
07/01/05-06/30/12 $121,037Mouse and Guinea Pig Models for HerpesvirusesNational Institutes of Health
09/27/10-09/26/11 $371,610EMMES/VRC700/BernsteinNational Institutes of Health(The Emmes Corporation)
11/15/10-12/31/11 $180,000Vaccine and Treatment Evaluation Units (VTEUs)National Institutes of Health
11/01/07-10/31/14 $1,761,446
HOSTETTER, M
Pediatric Physician Scientist Program AwardNational Institutes of HealthK12 HD 000850 09/01/10-06/30/12 $1,895,140PSDP American Academy of Pediatrics CommitmentAmerican Academy of Pediatrics
09/01/10-06/30/11 $57,375PSDP March of Dimes Funding CommitmentMarch of Dimes National
09/01/10-06/30/11 $6,823PSDP American Pediatric Society Funding CommitmentAmerican Pediatric Society
09/01/10-06/30/12 $44,955
JIANG, X
Characterization of Human CalicivirusesNational Institutes of HealthR01 AI 037093 09/01/09-08/31/11 $284,943Norovirus and Their ReceptorsNational Institutes of HealthR56 AI 055649 09/07/10-08/31/11 $261,891Novel Vaccine Against NorovirusNational Institutes of HealthR01 AI 089634 05/15/10-04/30/15 $761,012Novel Broad Spectrum Therapeutic Glycans Against Category B PathogensNational Institutes of Health(Boston College)U01 AI 075563 09/01/10-08/31/11 $75,154
MCNEAL, M
Hyaluronan Regulation of Microbial Host Defense of the IntestineNational Institutes of Health(Cleveland Clinic Lerner College of Medicine of Case Western Reserve University)R01 HD 061918 09/15/09-07/31/11 $30,497Subagreement GAT.1334-07574-SUBPATH Vaccine Solutions
11/15/07-10/31/11 $64,637Laboratory ServicesSerum Institute of India LTD
03/21/11-03/20/12 $144,000
SAWTELL, N
Unbiased Forward Genetic Analysis of Virus/Host InteractionsNational Institutes of HealthRC1 AI 087336 09/26/09-08/31/11 $339,996Ocular HSV Infection-Latency and PathogenesisNational Institutes of Health(University of Cincinnati)R01 EY 013168 05/01/08-04/30/12 $96,499
STAAT, M
Enhanced Surveillance for New Vaccine Preventable DiseasesCtr for Disease Control and PreventionU01 IP 000147 08/31/07-08/30/11 $1,340,299
Current Year Direct $7,837,314
Industry Contracts
BERNSTEIN, D
LigoCyte Pharmaceuticals, Inc $177,716MedImmune Inc. $102,616EMMES $71,610
BRADY, R
Accelovance, Inc. $10,420
CARDIN, R
Novartis Pharmaceuticals $170,214REPLICor, Inc. $15,215Genocea Biosciences, Inc. $107,489
DAWODU, A
Procter & Gamble China Scholars Program $240,000
FRENCK, R
Wyeth Pharmaceuticals $186,324GlaxoSmithKline $30,008
GERBER, M
Hoffman-LaRoche, Inc. $12,962
MCNEAL, M
Liquida Technologies, Inc $9,424
MORROW, A
Bristol-Myers Squibb $14,207
STAAT, M
GlaxoSmithKline $1,196,879
STEINHOFF, M
Wyeth Pharmaceuticals $84,992
WARD, R
Merck & Company, Inc. $428,816Sanofi Sythelabo $64,680Wyeth Pharmaceuticals $1,099Protein Sciences Corporation $106,519Research Triangle Institute $147,295
Current Year Direct Receipts $3,178,485
Total $11,015,799