Oct. 13, 2005 GW Canters, Leiden Univ 1

I . Substrates, immobilization

A. Substrates

B. Immobilization

a. surface preparation

b. attachment

Oct. 13, 2005 GW Canters, Leiden Univ 2

Substrates

Carbon (edge plane, basal plane)

Glassy carbon

Carbon nanotubes

TiO2

SiO2, Si

Au

I TO

Highly orientated pyrolytic graphite (HOPG)

Chris Chan, MetProt Grp. 2003

Oct. 13, 2005 GW Canters, Leiden Univ 4

Immobilization

Surface preparation

Attachment

Oct. 13, 2005 GW Canters, Leiden Univ 5

Langmuir 20(2004)8172

Oct. 13, 2005 GW Canters, Leiden Univ 6

Langmuir 20(2004)8172

Oct. 13, 2005 GW Canters, Leiden Univ 7

JACS 126(2004)8940

G. Ali Mansoori, “Principles of nanotechnology” (2005) World Scientific Publishers

Oct. 13, 2005 GW Canters, Leiden Univ 8

JACS 126(2004)8940

Oct. 13, 2005 GW Canters, Leiden Univ 9

Immobilization

Surface preparation

Attachment a. Non- bonding (Coulomb; v/dWaals) b. Covalent bonding c. H- bridges d. Co- factor reconstitution e. DNA- based techniques

Oct. 13, 2005 GW Canters, Leiden Univ 10

Oct. 13, 2005 GW Canters, Leiden Univ 11

2EtOH

H2O

(EtO)3SiCH2CH2CH2NH2

Si

O

O

OCH2CH3

CH2CH2CH2NH2

NH2

CH2CH2CH2-N=CH-CH2CH2CH2CHO

Si

OCH2CH3O

O

CH2CH2CH2-N=CH-CH2CH2CH2-C=N

Si

OCH2CH3O

O

OH

OH

H+

H2O

OHC-CH2CH2CH2-CHO

Oct. 13, 2005 GW Canters, Leiden Univ 12

JACS 127(2005)2018

Oct. 13, 2005 GW Canters, Leiden Univ 13

Oct. 13, 2005 GW Canters, Leiden Univ 14

JACS 120(1998)4478JACS 124(2002)9591

Oct. 13, 2005 GW Canters, Leiden Univ 15

Eur. J. Org. Chem. (1999)539

Oct. 13, 2005 GW Canters, Leiden Univ 16

Angew.Chem. 43(2004)6042

Langmuir 20(2004)8090

Chemical modication

Chemical modification