Download - Hardik free radical
![Page 1: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/1.jpg)
A seminar on
Oxygen free radicals and their Scavengers
ByHardik G. DaveIst year M. PharmHSKCOP, Bagalkot
![Page 2: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/2.jpg)
Introduction
It is believed that life has originated from basic
chemicals by free radical reaction, largely
initialled by ionising radiation from sun.
Paradoxically the same reactions creating life
may also be responsible for many diseases,
ageing and death.
![Page 3: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/3.jpg)
Free radical - what is it?• Free radical is an atom or group of atoms with
at least one unpaired electron.• Example :
• In the body it is usually an oxygen molecule that has lost an electron and will stabilize itself by stealing an electron from a nearby molecule.
• In the body free radicals are high-energy particles that ricochet wildly and damage cells.
Hydroxyl radical ( OH)O
H
![Page 4: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/4.jpg)
Radicals can be formed by…
1. The LOSS of a single electron from a non-radical, or by the GAIN of a single electron by a non-radical. And
2. The breakage of covalent bond ‘homolytic fission’
AB A + B
• Example H2O H + OH
![Page 5: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/5.jpg)
• These are highly reactive chemical entities that have a single unpaired electron in their outer most orbit.
• Under certain conditions can be highly toxic to the cells.• Generally unstable and try to become stable, either by
accepting or donating an electron.• Therefore if two free radical react, they neutralise each
other.• However, if the free radical react with stable molecules,
there is generation of more free radicals.• This character enables the FRs to participate in auto
catalytic chain reactions, – Molecules with which they react are themselves converted to
free radicals to propagate the chain of damages.
![Page 6: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/6.jpg)
• Any free radical involving oxygen can be referred to as reactive oxygen species (ROS).
• Oxygen centered free radicals contain two unpaired electrons in the outer shell.
• When free radicals steal an electron from a surrounding compound or molecule, a new free radical is formed in its place.
• In turn the newly formed radical then looks to return to its ground state by stealing electrons with antiparallel spins from cellular structures or molecules.
• These results in damaging the cell membranes. It also responsible for more than 100 human diseases like cancer, heart attacks, stroke and arthritis
![Page 7: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/7.jpg)
• TYPES OF FREE RADICALS :• The most common ROS include: i. the superoxide anion (O2-), ii. the hydroxyl radical (OH ·), iii. singlet oxygen (1O2 ), and iv. hydrogen peroxide (H2O2)
![Page 8: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/8.jpg)
1. SUPEROXIDE ANION (O2-) :• Superoxide anions are formed when oxygen (O2) acquires an
additional electron, leaving the molecule with only one unpaired electron. Within the mitochondria.
• O2- ·is continuously being formed.2. HYDROXYL RADICAL (OH- .) :• Hydroxyl radicals are short-lived, but the most damaging radicals
within the body.• This type of free radical can be formed from O2- and H2O2 via
the Harber-Weiss reaction.• The interaction of copper or iron and H2O2 also produce OH · as
first observed by Fenton. 3. HYDROGEN PEROXIDE (H2O2) :• Hydrogen peroxide is produced in vivo by many reactions. • Hydrogen peroxide is unique in that it can be converted to the
highly damaging hydroxyl radical or be catalyzed and excreted harmlessly as water.
![Page 9: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/9.jpg)
• Glutathione peroxidase is essential for the conversion of glutathione to oxidized glutathione, during which H2O2 is converted to water (2).
• If H2O2 is not converted into water 1O2 (singlet oxygen) is formed.
4. SINGLET OXYGEN (1O2) :• Singlet oxygen is not a free radical, but can be formed during
radical reactions and also cause further reactions.• When oxygen is energetically excited one of the electrons can
jump to empty orbital creating unpaired electrons.• Singlet oxygen can then transfer the energy to a new molecule
and act as a catalyst for free radical formation.• The molecule can also interact with other molecules leading to
the formation of a new free radical.
![Page 10: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/10.jpg)
PHYSIOLOGICAL EFFECTS :• Under normal conditions (at rest) the antioxidant defense system
within the body can easily handle free radicals that are produced. • During times of increased oxygen flux (i.e. exercise) free radical
production may exceed that of removal ultimately resulting in lipid peroxidation.
• Free radicals have been implicated as playing a role in the - etiology of cardiovascular disease, cancer, Alzheimer's disease, and Parkinson's disease.
![Page 11: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/11.jpg)
THE ROLE OF OXYGEN FREE RADICALS :
• Our bodies use oxygen to convert food items such as fat and sugar into energy, in this process, oxygen is converted to water, and each water molecule normally takes up four electrons.
• However some oxygen may escape before the conversion is complete, and this results in about 2% of the oxygen having an electron deficit.
• They may also be formed in the human body by air pollution, smoking and exposure to radiation.
• Free oxygen radicals are extremely reactive and can cause damage to body proteins and fats, and also to the hereditary material of cells, known as DNA.
• Fortunately our bodies have some protective mechanisms against the harmful effects of free oxygen radicals These are enzymes and antioxidants.
![Page 12: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/12.jpg)
• In mitochondria:- generation of energy - ATP- glucose, fatty acids, amino acids- O2 2H2O
4e-
- leakage of O2-. (superoxide)
H2O2 (hydrogen peroxide)
• In Smooth Endoplasmic Reticulum (micro some)- detoxification (cytochrome P-450s)- toxins, drugs and xenobiotics- O2 + RH R-OH and H2O
- leakage of O2-.
- metabolic activation - X.
![Page 13: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/13.jpg)
Leaking’ of electron (to oxygen) during electron transport leads to the formation of O2
- (O2 + e- O2-)
Superoxide Production from Mitochondrial Electron Transport Chain
![Page 14: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/14.jpg)
• • • •
• NADPH
• NADP+
• O2
• O2-
• NADPH
• NADP+
• O2
• O2-
• outside• inside
• .• .• .• .
• Phagocytic vacuole (phagosome)
Electron is transferred from NADPH to O2, resulting in the formation of O2-
•Formation of NADPH oxidase complex
Oxygen burst (respiratory burst) during phagocytosis
![Page 15: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/15.jpg)
• In Peroxisomes- containing oxidases for degradation of various substrates- glucose, amino acids, xanthine, etc.- requires O2
- by product is H2O2
• In Cytoplasm- nitric oxide (NO.) production from Arginine- functions as a biological messenger- in brain, vascular endothelial cells, and macrophages
- NO. + O2-. ONOO. (peroxynitrite)
![Page 16: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/16.jpg)
• Other source of free radical– Ionizing radiation, Ultraviolet radiation ,Ultrasound
– Chemicals, tobacco smoke, etc
![Page 17: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/17.jpg)
Roles of Free Radicals in Biological Systems
• Enzyme-catalyzed reactions• Electron transport in mitochondria• Signal transduction & gene expression• Activation of nuclear transcription factors• Oxidative damages of molecules, cells, tissues• Antimicrobial actions• Aging & diseases
![Page 18: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/18.jpg)
1.Oxidative stress :• Damages caused by free radicals/reactive oxygen species• Cellular damages at different levels (membrane, proteins,
DNA, etc) lead to cell death, tissue injury, cellular toxicity, etc• Reduction of antioxidants
2. Free Radical Toxicity :• Causes of free radical toxicity
– Increase production of free radicals– Decrease level of defense system (e.g.,antioxidants)
• Lipid peroxidation• DNA damage• Protein oxidation
![Page 19: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/19.jpg)
Lipid Peroxidation1. Initiation of first-chain reaction
• Abstraction of H+ by ROS (•OH)• Formation of lipid radical (LH•)• Formation of peroxyl radical (LOO•, ROO•)
2. Propagation• H+ abstraction by lipid peroxyl radical (LOO•)
3. Termination • Radical interaction non-radical product
![Page 20: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/20.jpg)
•
Molecular rearrangement
Conjugated diene
Lipid hydroperoxide
Cyclic peroxide
Cyclic endoperoxide
••-H
Hydrogen abstractionI
•
O
O
O
O
H
•H
O2 Oxygen uptake
Peroxy radical: abstractH• from another fatty acidcausing an autocatalytic chain reactions
P
I
P
Initiation
Propagation
(LH•)
(LOO•)
![Page 21: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/21.jpg)
Oxidative DNA Damage• Correlation with cancers and diseases• Oxidative DNA lesions by
-Direct attack
-Indirect activation of endonuclease enzymes
• Oxidative modification of bases – mutation• Oxidative modification of sugar moieties –
DNA strand
break
![Page 22: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/22.jpg)
• Abstraction of H+ atom from carbon atoms of sugar molecules• Disproportionations and rearrangement lead to C-C bond fragmentation and DNA strand break
A computer image depicts a hydroxyl radical attacking the sugar on the back bone of a DNA molecule
![Page 23: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/23.jpg)
Protein Oxidation
• Protein targets– Receptors, transport proteins, enzymes, etc– Secondary damage – autoimmunity
• Protein oxidation products– Protein carbonyl group, 3-nitrotyrosine, other
oxidized amino acids• Most susceptible amino acids
– Tyrosine, histidine, cysteine, methionine
![Page 24: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/24.jpg)
Antioxidant/Scavengers
![Page 25: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/25.jpg)
Damage(Pro-oxidants)
Defense(Antioxidants)
Oxidant-Antioxidant Balance
Damage(Pro-oxidants)
Defense(Antioxidants)
Decrease of antioxidant defense system
Oxidative damage
![Page 26: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/26.jpg)
Cellular Defense Mechanisms
• Isolation of generation sites of reactive oxygen species
• Inhibition of propagation phase of reactive oxygen species
• Scavenging of reactive oxygen species• Repair of the damage caused by reactive
oxygen species
![Page 27: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/27.jpg)
Protection Against ROS Damage
• Direct protection against ROS– Superoxide dismutase, Glutathione peroxidase, Catalase
• Non-specific reduction system– Glutathione, Vitamin C
• Protection against lipid peroxidation– Glutathione peroxidase, Vitamin E, -Carotene
• Sequestration of metals– Transferrin, Lactoferrin, Ferritin, Metalothionein
• Repair systems– DNA repair enzymes, Macroxyproteinases, Glutathione transferase
![Page 28: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/28.jpg)
Superoxide Dismutase (SOD)2O2
. - + 2H+ H2O2 + O2
• SOD - is present in all oxygen-metabolizing cells, different cofactors (metals).
• An inducible in case of superoxide overproduction.
1. CuZn-SOD – Cytoplasm, nucleus, lysosomes
2. Mn-SOD – Mitochondrial matrix
3. EC (CuZn) – Plasma membrane, extracellular
4. EC Mn-SOD – Plasma membrane
![Page 29: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/29.jpg)
Glutathione/Glutathione Peroxidase
![Page 30: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/30.jpg)
Catalase
2 H2O2 2 H2O + O2
• High affinity to H2O2 : peroxisomes hepatocytes mitochondria, cytoplasm of erytrocytes.
• Tetramer with Fe, needs NADPH.• Prevents lipid peroxidation and protein oxidation.
![Page 31: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/31.jpg)
Summary
![Page 32: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/32.jpg)
Melatonin• Highly selective & electroactive endogenous indoleamine.• Present in good amounts in the nervous system.• Sacrificed & irreversibly oxidized.• Inhibit NOS• Stimulate brain GPX activity• Increases mRNA levels for Mn & Cu-Zn SOD
![Page 33: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/33.jpg)
Ascorbic AcidAntioxidant Function• Donate 1 e- semidehydroascorbate (ascorbyl radical)
Relatively unreactive Tocopherol• Scavenges peroxyl radical• Inhibits chain reaction of lipid peroxidation• Chain-breaking antioxidant
![Page 34: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/34.jpg)
REFERENCE
• Text book of Pharmacotherapy and physiological approach , JOSHEP T DIPIRO, 5th edition, pg no – 390 and 1085.• Harpers Biochemistry 26th edition.• Lehninger's Principles of Biochemistry 4th Edition - D L
Nelson, Cox Lehninger - W H Freeman 2004.• Free-Radical-Induced DNA Damage and Its Repair - A
Chemical Perspective (Springer, 2006)• Rang and Dale’s Pharmacology , 6th edition • www.google.com
![Page 35: Hardik free radical](https://reader033.vdocuments.us/reader033/viewer/2022061611/5576d9d3d8b42a87588b4b35/html5/thumbnails/35.jpg)