Geometric Geometric morphometricsmorphometrics as a as a useful tool for visualising and useful tool for visualising and analysing deformities in fishanalysing deformities in fish
D. AdriaensD. Adriaens11, Y. Verhaegen, Y. Verhaegen11, T. De Wolf, T. De Wolf22, P. , P. DhertDhert33 & P. Sorgeloos& P. Sorgeloos44
1 Evolutionary Morphology of Vertebrates (UGent, Belgium)2 Maricoltura Rosignano Solvay (Italy)3 INVE Technologies (Dendermonde, Belgium)4 Artemia Reference Centre (UGent, Belgium) [email protected]
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 2/222/22
Why “morphometrics”?Why “morphometrics”?•• Measuring biological variationMeasuring biological variation
–– growth growth -- developmentdevelopment–– interspecific variation interspecific variation –– speciesspecies--specific featuresspecific features–– intraspecific variation intraspecific variation –– phenotypic plasticityphenotypic plasticity–– deformationsdeformations
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 3/223/22
Traditional “morphometrics”Traditional “morphometrics”•• Length measurementsLength measurements
–– advantagesadvantages•• easy to measureeasy to measure•• easy to analyseeasy to analyse
–– PCA, DFA, ...PCA, DFA, ...
–– disadvantagesdisadvantages•• size variables, not shape variablessize variables, not shape variables•• unsufficient model of shapeunsufficient model of shape
ab
a=a’b=b’
a’
b’
‘shapes’ are equal
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 4/224/22
Traditional “morphometrics”Traditional “morphometrics”•• Length ratiosLength ratios
–– advantagesadvantages•• standardised, size removalstandardised, size removal
–– disadvantagesdisadvantages•• reduces variationreduces variation•• size removal incompletesize removal incomplete
–– always dependent of sizealways dependent of size
•• size removal erroneous
ab
e
dc
size removal erroneous
a’
‘shapes’ are completely different
b’e’
d’c’
a≠a’ = standard lengthb=b’c=c’d=d’e=e’
b/a ≠b’/a’c/a ≠c’/a’d/a ≠d’/a’e/a ≠e’/a’
Solution to the problems?Solution to the problems?
Geometric MorphometricsGeometric Morphometrics
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 6/226/22
Data typesData types•• OutlinesOutlines
–– advantagesadvantages•• very good model of true shapevery good model of true shape
–– especially rounded shapesespecially rounded shapes
•• mathematical modelmathematical model–– shape parametersshape parameters–– statistics allowedstatistics allowed
PCA, DFA, ...PCA, DFA, ...
–– disadvantagesdisadvantages•• not good for certain shapesnot good for certain shapes
–– shape changes within the shape changes within the outline modeloutline model
–– shapes with pointed outlinesshapes with pointed outlines
•• no link to actual shape no link to actual shape variation in specimensvariation in specimens
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 7/227/22
Data typesData types•• LandmarksLandmarks
–– advantagesadvantages•• good model of true shapegood model of true shape
–– homologous pointshomologous points–– also shapes within shapesalso shapes within shapes
•• mathematical modelmathematical model–– shape parametersshape parameters–– statistics allowedstatistics allowed
PCA, DFA, ...PCA, DFA, ...
•• deformation gridsdeformation grids–– visualisation of shape visualisation of shape
differencesdifferences
–– disadvantagesdisadvantages•• not good for certain shapesnot good for certain shapes
–– rounded shapesrounded shapes
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 8/228/22
LandmarkLandmark--based morphometricsbased morphometrics•• Thin Plate SplinesThin Plate Splines
–– datadata•• Cartesian coordinatesCartesian coordinates
–– 2D, 3D
X
X
Y
Y
2D, 3D
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 9/229/22
LandmarkLandmark--based morphometricsbased morphometrics•• Thin Plate SplinesThin Plate Splines
–– datadata•• Cartesian coordinatesCartesian coordinates
–– 2D, 3D2D, 3D
–– standardisationstandardisation•• Generalised Procrustes Generalised Procrustes
AnalysisAnalysis–– sizesize–– rotationrotation–– position position
•• only true shape remains
X
Y
Y
only true shape remains
X
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 10/2210/22
Thin Plate SplinesThin Plate Splines•• Shape variation decompositionShape variation decomposition
–– reference shapereference shape•• consensusconsensus
–– new shape variablesnew shape variables•• partial warpspartial warps
–– uniform shape variationuniform shape variationcompressioncompressionshearshear
–– nonnon--uniform shape variationuniform shape variationoverall deformationoverall deformationlocalised deformationlocalised deformation
•• partial warp scorespartial warp scores–– weight matrixweight matrix
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 11/2211/22
Thin Plate SplinesThin Plate Splines•• Shape variation analysisShape variation analysis
–– Relative warp analysisRelative warp analysis•• PCA on partial warp scoresPCA on partial warp scores•• visualisation of shape variationvisualisation of shape variation
–– DFADFA•• on partial warp scoreson partial warp scores•• betweenbetween--group shape differencesgroup shape differences
RW1
RW2
min. RW1 min. RW1max. RW1
max. RW1
Geometric morphometricsGeometric morphometrics
Application for studying Application for studying deformations in deformations in Sparus aurataSparus aurata
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 13/2213/22
Data acquisitionData acquisition•• Digital imagesDigital images
–– specimens specimens nn=40=40
•• Landmark digitisationLandmark digitisation–– number of landmarksnumber of landmarks
•• 26 (type 1 & 2)26 (type 1 & 2)
•• GPAGPA–– consensusconsensus–– specimensspecimens
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 14/2214/22
Normal
Deoperculated
Data analysisData analysis•• Partial warp decompositionPartial warp decomposition
–– uniform variationuniform variation•• no clusteringno clustering
–– nonnon--uniform variationuniform variation•• partial warp 9partial warp 9
–– normal and deoperculated normal and deoperculated clustercluster
–– normal less variationnormal less variation
•• partial warp 12partial warp 12–– normal and deoperculated normal and deoperculated
clustercluster–– normal less variationnormal less variation
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 15/2215/22
Data analysisData analysis•• Relative warp analysisRelative warp analysis
–– RW1 (60.25%)RW1 (60.25%)
Normal
Deoperculated
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 16/2216/22
Data analysisData analysis•• Relative warp analysisRelative warp analysis
–– RW2 (9.37%)RW2 (9.37%)
Normal
Deoperculated
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 17/2217/22
Data analysisData analysis•• Relative warp analysisRelative warp analysis
–– RW3 (7.38%)RW3 (7.38%)
–– RW4 (4.67%)RW4 (4.67%)Deoperculated
Normal
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 18/2218/22
Trends in shape changesTrends in shape changes•• Normal to deoperculatedNormal to deoperculated
–– shape changesshape changes
–– not size relatednot size related–– distrupt variationdistrupt variation
•• developmental treshold?developmental treshold?•• early onset of deformation?early onset of deformation?
Normal
Normal
Deoperc.
Deoperculated
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 19/2219/22
Trends in shape changesTrends in shape changes
Normal
Deoperculated
•• NormalNormal–– shape changesshape changes
–– not size relatednot size related–– gradual variationgradual variation
•• phenotypic plasticity?phenotypic plasticity?•• deformities?deformities?
min
max.
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 20/2220/22
Trends in shape changesTrends in shape changesmin
max.
•• DeoperculatedDeoperculated–– shape changesshape changes
–– not size relatednot size related–– gradual variationgradual variation
•• phenotypic plasticity?phenotypic plasticity?•• variation larger than normalvariation larger than normal
–– different than normal phenotypic different than normal phenotypic plasticityplasticity
Normal
Deoperculated
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 21/2221/22
LeftLeft--right asymmetryright asymmetry•• Specimen C1Specimen C1--88
–– right right -- #30#30•• normalnormal
–– left left -- #9#9•• strongly deoperculatedstrongly deoperculated
right
left
March 11 2005March 11 2005 Workshop "Deformities in Fish Larvae"Workshop "Deformities in Fish Larvae" 22/2222/22
ConclusionsConclusions•• Use of geometric morphometricsUse of geometric morphometrics
–– more solid shape descriptorsmore solid shape descriptors–– more correct standardisation (GPA)more correct standardisation (GPA)–– statistical analyses applicablestatistical analyses applicable–– visualisation of shape variationvisualisation of shape variation
•• Deformations in Deformations in Sparus aurataSparus aurata–– geometric morphometrics very usefulgeometric morphometrics very useful–– ‘shape‘shape--gap’ between normal and deoperculatedgap’ between normal and deoperculated–– withinwithin--group variation not size relatedgroup variation not size related–– extreme leftextreme left--right asymmetryright asymmetry