FOOD & DIETARY SUPPLEMENTSDirectory of Services
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NL-Proximate (moisture, ash, protein)
Calories (by calculation)
Calories from fat (by calculation) *Requires fat, moisture, protein and ash testing
Total fat [sum of fatty acids (saturated, monounsaturated, polyunsaturated, trans)]
Cholesterol
Total carbohydrates (by calculation) *Requires fat, moisture, protein and ash testing
Total dietary fiber
Sugars HPLC GC Either HPLC or GC will be used, depending on the sample matrix.
Vitamin A
beta Carotene If there is a source of beta carotene, the assay for beta carotene should be used instead of (or in addition to) vitamin A to obtain total vitamin A activity for nutrition labels.
Vitamin C
Calcium, iron, sodium (ICP)
Mandatory Nutrient PackageThis package includes all nutrients, with sugars assayed by GC, minerals by ICP and total fat as the sum of the fatty acids calculated as triglycerides.
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NUTRITION LABELING MANDATORY NUTRIENT LIST
Regulatory initiatives such as the Nutrition Labeling and Education Act have had a profound impact on the food industry. The adoption of standardized content and format guidelines for nutrition labeling have provided consumers with the tools to plan a balanced diet, as well as given producers data to distance their product from the competition.
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AstaxanthinBiotinCarotenoids Option 1: alpha Carotene, beta Carotene, Lycopene Option 2: Cryptoxanthin, Lutein, Zeaxanthin Option 3: Lutein onlyCholine (chemical)Choline (enzymatic)Cyanocobalamin (vitamin B12)Folate (vitamin B9)InositolL-Ascorbyl-2-PhosphateNiacin (vitamin B3)Pantothenic acid (vitamin B5)Pyridoxine (vitamin B6)
Riboflavin (vitamin B2)Thiamin (vitamin B1)Tocopherols (alpha, beta, gamma and delta)Tocotrienols (alpha, beta, gamma and delta)Vitamin A modified USP for dietary supplements (HPLC)Vitamin A, retinolVitamin B profile (HPLC)Vitamin B profile (HPLC-MS/MS)Vitamin CVitamin DVitamin E (alpha tocopherol) Vitamin E (in feeds and forages)Vitamin K1
VITAMIN ANALYSIS
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CholesterolConjugated linoleic acid (CLA)Fatty acid profile (C4 - C24) (including Omega-3 and Omega-6 isomers, and trans fatty acids)Fatty acid profile (C4 - C24) (including Omega-3 and Omega-6 isomers, not including fatty acids)Free fatty acids (total by titration)Free fatty acids (by GC)
GlycerolHexanalIodine valuep-Anisidine Peroxide valueSterolsTBA value
Acid detergent fiberAspartame, Acesulfame K, DKP and saccharine beta GlucanCrude fiberGalactooligosaccharides (GOS) content in Infant FormulaGalactooligosaccharides (GOS) content in Raw MaterialInsoluble and soluble fiber (Lee Method) Insoluble, soluble and total dietary fiber (CODEX definition) by enzymatic-gravimetric method and liquid
chromatography (McCleary)Inulin (fructan), HPLCInulin (fructan), spectrographicLactuloseLigninNeutral detergent fiber (enzymatic)PolydextroseResistant starchStarch
Sucralose (finished products)Sucralose (packets, pure material) (USP Method)Sugar alcoholsSugar profile after acid hydrolysisSugar profile by GCSugar profile, low levelsSugar profile by HPLCSugar profile by ICTotal dietary fiber (CODEX definition) by enzymatic-gravimetric method and liquid
chromatography (McCleary) Total dietary fiber containing supplemented resistant maltodextrin (Fibersol)Total dietary fiber (Lee or Prosky)
LIPIDS
CARBOHYDRATES
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Total amino acid profile (AOAC) Alanine, arginine, aspartic acid (including asparagine),
cystine, glutamic acid (including glutamine), glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, valine
Total amino acid profile (HPLC) Alanine, arginine, aspartic acid (including asparagine),
cystine (including cysteine), glutamic acid (including glutamine), glycine, histidine, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, valine, tryptophan
Free amino acid profile (AOAC) Alanine, arginine, aspartic acid (including asparagine),
cystine, glutamic acid (including glutamine), glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, valine
Free amino acid profile (HPLC) L-aspartic acid, L-glutamic acid, L-serine, L-histidine,
glycine, L-threonine, L-arginine, L-alanine, L-tyrosine, L-cystine, L-valine, L-methionine, L-phenylalanine, L-isoleucine, L-leucine, L-lysine, proline, L-asparagine, L-glutamine, L-citrulline, L-tryptophan, hydroxyproline
5-Hydroxytrytophan (5-HTP)beta AlanineCarnitineCysteineCystine and Methionine (AOAC) GABA (gamma aminobutyric acid)Monosodium glutamate (as glutamic acid) N-Acetyl-L-CysteineOrnithineTaurine (total and free)TheanineTryptophan (AOAC)Tryptophan (HPLC) (total and free)
AMINO ACIDS
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Organic Acid Profiles Option 1: Benzoic acid (sodium benzoate), sorbic acid (potassium sorbate)
Option 2: Citric acid, malic acid, lactic acid, acetic acid, oxalic acid, pyruvic, quinic acid Option 3: Tartaric acid, formic acid, succinic acid, fumaric acid Option 4: Propionic acid
ORGANIC ACIDS
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Acid insoluble ashAsh Available carbohydrates (direct determination)Bomb calorimetryDensityFat extractions Acid hydrolysis
Methanol/chloroform Roese-Gottlieb Soxhlet
Fat (sum of fatty acids)Loss on drying (LOD)
Moisture (Karl Fischer)Moisture (vacuum oven)pHProtein (Dumas)Protein (Kjeldahl)Proximate package (moisture, ash, protein and fat) Calories (by calculation)
Total carbohydrates (by calculation)Residue on ignition (ROI)Titratable acidityWater activity
Alcohol (added) Alcohol (residual)BHA, BHT and TBHQ (antioxidants)Caffeine, theobromine and theophyllineCapsacin/capsacinoid (Scoville heat)DissolutionEthanol and MethanolNucleosidesNucleotides
OsmolalityParabens (methyl, ethyl, propyl, butyl, isopropyl, isobutyl)PolysorbatesResidual solventsSoy isoflavones (genistein, daidzein, glycitein)Species Identification by ELISA (beef, poultry or pork)Sulfites (Monier-Williams) TBHQViscosity (Brookfield or Bostwick)
Allergens Almond
Brazil Nut Casein Cashew Kernel Celery Coconut Crustaceae Shellfish Egg Gluten Hazelnut Lupine Macadamia Nut Mustard Peanut Pistachio Sesame Soy Allergen Total Milk Walnut
Other Contaminants 3-MCPD (3-Chloro-1,2-Propanediol)
4-MEI Acrylamide by LC-MS/MS Aflatoxins by HPLC (B1, B2, G1, G2) Aflatoxins (B1, B2, G1, G2), Ochratoxin A and Zearalenone by HPLC
Aflatoxins M1 by HPLC Arsenic Speciation Bisphenol A (BPA) Cyanuric acid by LC-MS/MS Heavy metals by ICP (arsenic, cadmium, lead, mercury) Heavy metals by ICP-MS (arsenic, cadmium, lead, mercury) Melamine by LC-MS/MS Melamine and analogs by UHPLC-MS/MS Mycotoxins in infant formula (Aflatoxin B1, B2, G1, G2, M1, M2, deoxynivalenol, Ochratoxin A, Zearalenone,
Fumonisin B1, B2, HT-2, T-2 toxin)Polycyclic aromatic hydrocarbons (PAH) Residual solvents
PROXIMATE
OTHER ASSAYS
NATURAL CONTAMINANTS
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ICP Aluminum Barium Beryllium Boron Cadmium Calcium Chromium Cobalt Copper Iron Magnesium Manganese Molybdenum Nickel Phosphorus Potassium Sodium Strontium Vanadium Zinc
ICP-MS Antimony Arsenic Barium Beryllium Bismuth Cadmium Caesium Calcium Chromium Cobalt Copper Gallium Germanium Iodine Iron Lanthanum Lead Lithium Magnesium Manganese
Mercury Molybdenum Nickel Palladium Platinum Rubidium Ruthenium Selenium Silver Strontium Sulfate* Sulfur Thallium Tin Titanium Tungsten Vanadium Zinc
INDIVIDUAL ELEMENTS
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INORGANIC ANALYSISThe method used for the analysis of various elements in food and dietary supplement samples is dependent on both the concentration and the sample matrix. For most elements the precision using any of these techniques is less than 5% RSD.
Inductively Coupled Plasma Spectroscopy (ICP) is used for macro-levels inorganic constituents in all matrices. Usually 10g of sample is either dry or wet ashed, or samples are analyzed directly. The limit of quantitation is based on the individual analyte and matrix, but generally yields detection in the PPM range. A common scan is for calcium, iron and sodium used for nutritional labeling.
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) is the method of choice when analyzing trace level analytes. It is applicable to all matrices. Usually 0.5 g of sample is wet ashed in a closed-vessel microwave digestion, although other ashing techniques are used. Limits of quantitation are lower than ICP and are dependent on the analyte and matrix, but generally yield detection in the PPB range. A common scan is for arsenic, cadmium, lead and mercury used for heavy metal analysis.
Atomic Absorption Spectrospcopy (AA) is used for single analyte measurements using flame, hydride-generation or graphite furnace techniques. This technology, in general, has been surpassed by the advances made in ICP and ICP-MS techniques. Please see the details in the assay section of the catalog for specifics.
Flame AA Silicon
Titanium Other analytes available on request include calcium, chromium (in fortified supplements), cobalt, copper, iron, lead, magnesium, manganese, molybdenum, potassium, selenium, sodium and zinc.
Graphite Furnace AA Aluminum
Other analytes, such as chromium, available on request
Inorganic Speciation IC-ICP-MS Arsenic
Miscellaneous AA Selenium (hydride)
* Calculated from total sulfur by ICP-MS
8 Pricing available from a Covance representative. For updates to assays, refer to our online catalog at covance.com/nutri.
Inorganics by ICP Emission Spectrometry Packages Option 1: ICP3–calcium, iron, sodium (for nutrition
labeling) Option 2: ICPL–calcium, copper, iron, magnesium,
manganese, phosphorus, potassium, sodium, zinc Option 3: ICPN–Option 2 plus aluminum, barium, boron,
chromium*, molybdenum*, strontium Option 4: ICPS–Option 3 plus beryllium, cadmium, cobalt,
nickel, vanadium* May need to perform an alternate test for certain matrices.
Inorganics by Wet Chemistry Chloride Fluoride (chemical) Fluoride (ISE) Iodine (chemical) Iodine (ISE) Nitrate/nitrite by HPLC Nitrate/nitrite by IC and VIS Heavy metals as lead by USP <231>
INORGANIC ANALYSIS
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MICROBIOLOGY SERVICES
Feeling challenged by the demands for increased food safety in today’s market place? Covance is ready to help you produce safe products and keep you compliant and competitive with our broad array of services that address consumer and regulatory requirements.
In anticipation of your needs, we have dedicated two ISO accredited laboratories (Battle Creek, Michigan and Madison, Wisconsin) to delivering microbiological results you can use to base food safety decisions. Along with testing capabilities, Covance offers microbiology consulting, customized research and training services.
Contact Us Today (1.855.83MICRO or 1.855.33MICRO)
Discover why Covance is your optimum partner in food safety services
CONSULTING• Investigations to help
identify, isolate and eliminate microbial harborage points
• Responses to regulatory actions
• Expert witness services• Food safety program
development and review • GFSI audit preparation
RESEARCH• White paper, laboratory and
in-plant process validations• Customized challenge studies
based on NACMCF guidelines• Pathogen- and spoilage-based
microbial shelf-life evaluations• Confirmation and/or validation of
cooking/baking instructions
TRAININGGeneral education sessions or
customized courses, held in our classrooms or delivered at your facility
• International HACCP Alliance accredited training classes and instructors
• Prerequisite programs• Internal audits• Sampling technique• Sampling site selection• Laboratory techniques• Other topics upon request
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MICROBIOLOGY ASSAYS
Food, Ingredients and Environmental Swabs
Quantitative – Counts or MPN
Aerobic plate Lactic Acid Bacteria
Bacillus cereus Lactobacillus - Heterofermentative
Chronobacter sakazakii Mesophilic aerobic spores
Coliform Mesophilic anaerobic spores
Coliform MPN Staphylococcus species
E. coli Staphylococcus species – Coagulase (+)
E. coli MPN Yeasts and molds
Enterobacteriaceae Yeasts and molds – Osmophilic
Additional charges for samples requiring dilution beyond 1/1000.
Qualitative – Presence/Absence
E. coli O157:H7 Listeria species
E. coli – STEC Listeria monocytogenes
Salmonella species
Additional charges for sample sizes > 100 g and for compositing of individual samples.
Confirmation/Identification
Gram stain Biochemical – VITEK and API
Dietary Supplements and Nutritional Products
USP
Verification of assay suitability Clostridia species
Total aerobic microbial count (TAMC) E. coli
Total combined yeasts and molds count (TYMC) Salmonella species
Bile-tolerant Gram negative bacteria Staphylococcus aureus
Candida albicans
Additional charges for dilutions beyond 1/10 and sample neutralization.
Non-USP
Probiotic enumerations Enumeration of microencapsulated microorganisms
Shelf-life viability Simulated gastric dissolution
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CONTAMINANTS IN FOODS, FEEDS AND DIETARY SUPPLEMENTS
Global distribution of food, feed and dietary supplement products into new regions requires a broad understanding of the interplay between growers, manufacturers and regulators. First-hand experience developing new testing methods, global regulatory knowledge and the scientific expertise to develop an appropriate testing program is critical to meeting your specific product needs. Our scientists have designed and managed hundreds of testing programs for heavy metals, acrylamide, melamine and pesticide residues in support of Proposition 65, response to economic adulteration, routine supplier verification and Infant Formula EU Directive 2006/141/EC, respectively.
Our Center of Excellence for Contaminants in Greenfield, Indiana, offers a broad portfolio of contaminant testing services. The 10,000 sq ft state-of-the-art facility in Greenfield provides clients the most up-to-date analysis of chemical residues and contaminants as well as the development of new analytical methods.
We approach the analysis of contaminants based on analytical quantitation using highly selective technology including ICP-MS, LC- and GC-MS/MS. As a true partner in food and dietary supplement analysis, we have the resources to meet your requirements for quality, flexibility, value and customer service.
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Multi-Residue Analysis (MRA) by GC-MS/MS and LC-MS/MS (300+ compounds)
Botanicals, Spices and Other Specialty Commodities and Products All analyses reported to 10 ppb or LOQ, whichever is higher.
Finished Food and Raw Ingredients All analyses reported to 10 ppb or LOQ, whichever is higher.
This offering includes: ▶ Fruits and vegetables ▶ Grains and cereals ▶ Fats and oils ▶ High-fat food products ▶ Finished processed foods, including meals
Infant Formula All analytes are reported to 10 ppb (or lower if required). EU Directive 2006/141/EC (10 ng/g or less) offering is available.
Matrix-Based Quantitation Additional analyses (after initial analysis) to perform Method of Standard Addition, which provides the most
accurate quantitation.
Individual Pesticides or Subgroups Individual pesticides, subgroups of or additions to the MRA.
Carbamates by LC-MS/MS
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USP/EP Analysis (99 Analytes)
USP 561 USP/EP Pesticide Full Reporting Package Report at the USP/EP limits. Full multipoint calibration for all analytes by GC-MS/MS and LC-MS/MS. Includes EBDCs
(dithiocarbamates) by GC, inorganic bromide by GC and matrix-based quantitation.*
* Matrix-Based Quantitation: additional analyses (after initial analysis) to perform Method of Standard Addition, which provides the most accurate quantitation.
Ala Cart Analysis
GC-MS/MS and LC-MS/MS Multi-Residue Analysis (select 1 to 90+ analytes)Matrix-based quantitationEBDCs (dithiocarbamates) by GCBromide, inorganic
COMPENDIAL TESTING SERVICES
Raw Material/Ingredient Analysis
At Covance, we have been performing monograph analyses of raw materials and ingredients for over 30 years. In accordance with many global regulations, our database of over 400 different raw materials and capacity to set up more provides you with the analytical services you need for your raw materials testing.
To expedite your analysis, please provide the following information in advance:▶ Quarterly or yearly list of raw materials▶ Notice of shipment▶ Analysis request form (available online or from Client Services)
This testing is compliant with GMP regulations (21 CFR Part 111) for ingredients in dietary supplements. For assays compliant with 21 CFR Part 211 for human drug ingredients, please contact us.
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CONTAMINANTS IN FOODS, FEEDS AND DIETARY SUPPLEMENTS
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STABILITY STUDIES: STABILITY AND SHELF-LIFE EVALUATION
Covance provides comprehensive analytical services related to stability and shelf-life of products for the food and dietary supplement industries. Stability is defined as the extent to which a product retains, within specified limits throughout its period of storage and use, the same properties and characteristics possessed at the time of packaging. Understanding product stability is an essential measure of the quality of a food or dietary supplement. Covance can custom design your stability study from start to finish.
Study Design - Essential Information for Stability Studies
▶ Packaging materials▶ Region the product is being sold▶ Product specifications▶ Type of study (accelerated or real time)▶ Shelf-life anticipated▶ Regulatory requirements (if applicable)▶ Method applicability
Testing Services for Stability Studies
▶ Chemical analysis (nutrients, active compounds)▶ Physical properties (moisture, water activity, disintegration,
hardness, etc.)▶ Organoleptic evaluation (appearance, taste, smell, color)▶ Microbiological evaluation (safety assessment,
antimicrobial preservative effectiveness)▶ Monograph testing (USP or International)
Goals of the Stability Study
▶ Ensure the product is stable over its shelf-life▶ Establish or verify the shelf-life of the product▶ Aid in product research and development▶ Provide data to support the quality of the product over time▶ Fulfill any regulatory requirements
Due to the custom nature of designing a stability study, please inquire on pricing and storage availability.
Typical Stability Studies
Testing Intervals Storage Study Condition (ICH guidelines)Real Time 25°C/60% RH 3, 6, 9, 12, 18, 24 monthsAccelerated 40°C/75% RH 3, 6 monthsAlternate 30°C/65% RH 6, 9, 12 months
PHYTOCHEMICALS
The terms phytochemicals and phytonutrients have been adopted as descriptors for certain organic components of plants that are thought to promote human health. Unlike the traditional nutrients, these compounds are not “essential” for life from a traditional standpoint but are being increasingly recognized as having beneficial health characteristics. As the popularity of these products has increased with mainstream consumers, the use of a variety of dietary supplements, herbals/botanicals and functional foods has expanded and resulted in an intense and competitive marketplace. The ability to scientifically substantiate claims for quality, purity, consistency, stability, safety and efficacy is an essential marketing tool and frequently a regulatory requirement.
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METHOD DEVELOPMENT, VALIDATION AND TRANSFER
Responding to changes in market or regulatory requirements demands new or improved high-quality scientific methods engineered for greater efficiency, lower limits of detection and improved reproducibility. As a partner in developing solutions to method challenges, Covance offers the services of our Technical Development team on a contract basis. You can leverage the team’s expertise across a wide range of matrices and technologies, including LC-MS/MS and UHPLC, to address your analytical challenges. Projects are priced on a time/materials or project basis.
Typical projects we have completed include:
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SPECIAL SERVICES
Colors
Artificial Colors
FD&C Red No. 2 (Amaranth)FD&C Red No. 3 (Erythrosin B)FD&C Red No. 4 (Ponceau SX)FD&C Red No. 40 (Allura Red)
FD&C Green No. 3 (Fast Green FCF)FD&C Blue No. 1 (Erigluacine)FD&C Blue No. 2 (Indigo Carmine)Acid Orange 12 (Crocein Orange G)
FD&C Yellow No. 5 (Tartrazine)FD&C Yellow No. 6 (Sunset Yellow FCF)FD&C Yellow No. 10 (Quinoline Yellow)
▶ New method development▶ Compendial/journal method setup▶ Matrix extensions (incl. validation)
▶ Client method validation▶ Existing method modernization
▶ New technology application▶ Client method variability resolution
Japanese Export Testing
Sennosides (UV)Fenfluramine, N-nitroso-fenfluramine (HPLC)p-Hydroxybenzoates (methyl, ethyl, propyl, butyl, isoproyl, isobutyl)Thryoxine, triiodothyronine (HPLC)Benzoic and sorbic acids
Prices for these tests are all product-dependent—specific pricing for your supplement product can be obtained by calling us at 800-675-8375.
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BOTANICALS
For centuries, people have used herbs and other botanicals for their healthful effects, possibly due to their phytochemical composition, and in recent years, the use of these products has experienced a rise in popularity among consumers. This has resulted in corresponding regulatory initiatives and the accelerated need for analytical methods for botanical products. Several regulatory initiatives, including changes to current Good Manufacturing Practices (cGMP), address identification testing for botanical ingredients.
FOOD CONTACT MATERIALS (MIGRATION COMPLIANCE)
The potential for migration of indirect additives and contaminants from food contact material is a concern for both food products and dietary supplements. As the design and use of innovative packaging methods change, there is a corresponding effect on the regulatory and experimental guidelines for determining the impact of packaging components on the safety of food and dietary products. In the case of dietary supplements and botanicals, migration could potentially have a negative effect on the efficacy of the active ingredient. When evaluating the safety of packaging materials there are three key aspects to be considered: the source of the materials, the nature of the manufacturing process and the conditions of use.
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▶ Total and chloroform-soluble extractables from food packaging
▶ Recycled and degradable packaging
▶ Retort pouch barrier integrity
▶ Pigment and dye migration from consumer products
▶ Benzene in polymeric packaging materials
▶ Microwave packaging
▶ End use testing verifying compliance with 21 CFR Parts 172-181
▶ Conditions of use A-J
▶ Experience with FCN and EU recommendations
For guidance, please refer to: www.packaginglaw.com
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DIETARY SUPPLEMENTS
Covance has over 80 years of experience in food analysis and has built a reputation for scientific and regulatory expertise, responsiveness to customer needs and the capacity to turn around samples quickly and efficiently. As a full-service laboratory, we analyze food, dietary supplement and biotechnology products.
Product/Analyte ....................................................................................................................................... Testing Method
5-Hydroxytryptophan (5-HTP) .......................................................................................................................................... HPLC
Acetyl Cysteine ................................................................................................................................................................... HPLC
Allicin (garlic) ..................................................................................................................................................................... HPLC
alpha Lipoic Acid (USP) .................................................................................................................................................... HPLC
Anthocyanins ..................................................................................................................................................................... HPLC
Anthocyanins (bilberry, elderberry, cranberry, fruits) ....................................................................................................... VIS
Astaxanthin ........................................................................................................................................................................ HPLC
beta-Glucan .......................................................................................................................................................................... VIS
Black Currant Oil (linoleic acid and GLA) ..........................................................................................................................GC
Caffeine, Theobromine, Theophylline ............................................................................................................................. HPLC
Capsaicin/Capsaicinoid (Scoville Heat) ........................................................................................................................... HPLC
Catechins (EGCG, GCG, catechin, EGC, EC, ECG, GC, CG) + gallic acid .................................................................... HPLC
Chlorogenic Acid ............................................................................................................................................................... HPLC
Chondroitin Sulfate by CPC (USP) ................................................................................................................................Titration
Citrus Bioflavanoids .......................................................................................................................................................... HPLC
Coenzyme Q10 ................................................................................................................................................................... HPLC
Conjugated Linoleic Acid (CLA) ..........................................................................................................................................GC
Cranberry (quinic, malic, citric acids) .............................................................................................................................. HPLC
Creatine .............................................................................................................................................................................. HPLC
Cryptoxanthin .................................................................................................................................................................... HPLC
DHEA or 7-KETO DHEA
(Dehydroepiandrosterone) or (3-acetyl-7-oxo-dehydropiandrosterone) ...................................................................... HPLC
Dong Quai (ferulic acid) .................................................................................................................................................... HPLC
Echinacea spp. (caftaric, chlorogenic, echinacoside, chicoric) ......................................................................................... HPLC
Echinacea spp. [total phenolics as gallic acid equivalents (GAE)] ...................................................................................... VIS
Elagic Acid .......................................................................................................................................................................... HPLC
Ephedrine Alkaloids
Ephedra or Ma Huang ................................................................................................................................................LC-MS/MS
Evening Primrose Oil (linoleic acid and GLA) ...................................................................................................................GC
Fo-ti Powder (trans-resveratrol) ........................................................................................................................................ HPLC
Fructan (AOAC 997.08) ............................................................................................................................................... HPAEC-PAD
Fructan (AOAC 999.03) ....................................................................................................................................................... VIS
Furfural............................................................................................................................................................................... HPLC
gamma Aminobutyric Acid (GABA) ................................................................................................................................ HPLC
gamma Linoleic Acid (GLA) .................................................................................................................................................GC
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DIETARY SUPPLEMENTS (CONTINUED)
Product/Analyte ....................................................................................................................................... Testing Method
Garcinia cambogia (hydroxycitric acid) ............................................................................................................................. HPLC
Garlic (allicin) ..................................................................................................................................................................... HPLC
Ginkgo Biloba Flavonoids ................................................................................................................................................. HPLC
Ginkgo Biloba Terpenoids ................................................................................................................................................. HPLC
Ginseng, Panax or Korean (ginsenosides) ....................................................................................................................... HPLC
Glucosamine .......................................................................................................................................................... HPLC/HPAEC-PAD
Glycerol (glycerine) ...............................................................................................................................................................GC
Goldenseal (hydrastine and berberine) ............................................................................................................................ HPLC
Grape Seed Extract
Catechins ......................................................................................................................................................................... HPLC
Total Polyphenols (Folin-Ciocalteu Method) ................................................................................................................. VIS
Proanthocyandins (polyphenols - catechins) ............................................................................................................ VIS/HPLC
trans-Resveratrol ............................................................................................................................................................. HPLC
Green Tea Catechins .......................................................................................................................................................... HPLC
Griffonia Seed Extract (5-Hydroxytryptophan) 5-HTP ..................................................................................................... HPLC
Guarana (theobromine, theophylline, caffeine) ............................................................................................................... HPLC
Hydroxycitric Acid (garcinia extract) ................................................................................................................................ HPLC
Isoflavones (daidzein, glycitein, genistein, daidzin, glycitin, genistin) ......................................................................... HPLC
Kudzu (isoflavones) ........................................................................................................................................................... HPLC
Lutein .................................................................................................................................................................................. HPLC
Lycopene ............................................................................................................................................................................. HPLC
Marine Lipids (EPA/DHA) ...................................................................................................................................................GC
Methylsulfonylmethane (MSM) ...........................................................................................................................................GC
Milk Thistle (silychristin, silydianin, silibibin A & B) ..................................................................................................... HPLC
Omega-3 Fatty Acids .............................................................................................................................................................GC
ORAC, Total (includes hydrophilic and lipophilic) ............................................................................................................ FL
Oxalic acid .......................................................................................................................................................................... HPLC
PABA (para-aminobenzoic acid) ....................................................................................................................................... HPLC
Phenolic Acid (Phenylproponoids) Caffeic, p-Coumaric, Sinapic, and Ferulic Acids ................................................... HPLC
Phospholipids (Lecithin Phospholipids)
Phosphatidic acid, phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, phosphatidylinositol ... HPLC
Phytic Acid .......................................................................................................................................................................... HPLC
Phytosterols (stigmasterol, beta sistosterol, campesterol, brassicasterol) ........................................................................GC
Policosanol (octacosanol, triacontanol, tetracosanol, hexacosanol)...................................................................................GC
Polydextrose ........................................................................................................................................................... HPLC/HPAEC-PAD
Polygonum cuspidatum (trans-resveratrol) ........................................................................................................................ HPLC
Polyphenols (Total Polyphenols) Folin-Ciocalteu Method ................................................................................................ VIS
Polyphenols (Total) with % Solids
Tea and tea products for Tea Association members only .............................................................................................. VIS
Pueraria (isoflavones) ........................................................................................................................................................ HPLC
Punicalagins ....................................................................................................................................................................... HPLC
DIETARY SUPPLEMENTS (CONTINUED)
Product/Analyte ....................................................................................................................................... Testing Method
Pygeum (beta sitosterol) .......................................................................................................................................................GC
Pyruvate (pyruvic acid) ...................................................................................................................................................... HPLC
Quercetin ............................................................................................................................................................................ HPLC
Rose Hips (ascorbic acid) ..................................................................................................................................................... FL
Royal Jelly 10-HDA (10-hydroxy-2-decanoic acid) ............................................................................................................ HPLC
Rutin ................................................................................................................................................................................... HPLC
SAMe (S-adenosylmethionine) ......................................................................................................................................... HPLC
Saw Palmetto (phytosterols + fatty acids) ............................................................................................................................GC
Scoville Heat (capsaicin and capsaicinoid) ....................................................................................................................... HPLC
Soy Isoflavones ................................................................................................................................................................... HPLC
Stinging Nettles (5-hydroxytryptophan) ........................................................................................................................... HPLC
Theobroma cacoa (caffeine, theobromine, theophylline) ................................................................................................. HPLC
Tocotrienols (alpha, beta, gamma, delta) ......................................................................................................................... HPLC
Tomato (lycopene) ............................................................................................................................................................. HPLC
trans-Resveratrol ................................................................................................................................................................ HPLC
Ubiquinone (Coenzyme Q10) ........................................................................................................................................... HPLC
Valerian Extract (valerenic acid) ........................................................................................................................................ HPLC
Yerba Mate (caffeine, theobromine, theophylline) .......................................................................................................... HPLC
Zeaxanthin ......................................................................................................................................................................... HPLC
18 Pricing available from a Covance representative. For updates to assays, refer to our online catalog at covance.com/nutri.
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