Download - Fluorescent Protein Fusion Expression System
-
8/8/2019 Fluorescent Protein Fusion Expression System
1/17
All l Bi t h I t d i C t Eff ti t R h
Fluorescent Protein
Fusion Expression System
For Research Use Only. Not for Diagnostic or Therapeutic Use. Purchase does not include or carry any right to resell or transferthis product either as a stand-alone product or as a component of another product. Any use of this product other than the permitteduse without the express written authorization of Allele Biotech is strictly prohibited
Box 1 | Product List
Fluorescent Protein Vectors
Vector Name Cat. Number
PagepmWasabi-C ABP-FP-WCNCS 2pmWasabi-N ABP-FP-WNNCS 3pmTFP1-C ABP-FP-TCNCS 4pmTFP1-N ABP-FP-TNNCS 5pLanRFP-C ABP-FP-RCNCS 6pLanRFP-N ABP-FP-RNNCS 7pLanYFP-C ABP-FP-YCNCS 8pLanYFP-N ABP-FP-YNNCS 9Vectors w/ Puromycin Resistance
pmWasabi-C-Puro ABP-FP-WCNCS 10pmWasabi-N-Puro ABP-FP-WNNCS 11pmTFP1-C-Puro ABP-FP-TCNCS 12pmTFP1-N-Puro ABP-FP-TNNCS 13pLanRFP-C-Puro ABP-FP-RCNCS 14pLanRFP-N-Puro ABP-FP-RNNCS 15pLanYFP-C-Puro ABP-FP-YCNCS 16pLanYFP-N-Puro ABP-FP-YNNCS 17
IMPORTANT NOTICE TO PURCHASER:These products may be subjects of pendingU.S. and foreign patents. Allele Biotech grants
academic/government research institutions aworldwide, non-exclusive, royalty-free, limited
license to use this product for non-commercial
life science research use only. Such license
specifcally prohibits the right to sell or otherwise
transfer this product or its derivatives to a third
party.
Industry For-Proft Institutions: Institutions that
wish to use this product are required to obtain asub-licensing agreement from Allele Biotech. For
more information please visit Fluorescent Protein
Sublicense, or contact us via phone or email.
-
8/8/2019 Fluorescent Protein Fusion Expression System
2/17
CMV Promoter ........ 1-589
Multiple Cloning Sites ........ 591-646
mWasabi GFP ........ 653-1363
SV40 poly(A) Signal ........ 1529-1579
Ampicillin Resistance Gene ........ 2207-3067
pUC Origin ........ 3216-3858
Sequencing Primer:
CMV-F and mWasabi-R(ABP-FP-WASRPRM)
pmWasabi-CT (ABP-FP-WCNCS)
Multiple Cloning Sites
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA
R * L *
601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC CTCGAGGCCA CCATGGTGAG
T G S S N S V P Q A C G R L E A T M V S
661 CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC ATGAAGATCA AGCTGAAGAT
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1321 GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG TAA
NheI
BamHI EcoRI KpnI HindIII NotI XhoI
C
-
8/8/2019 Fluorescent Protein Fusion Expression System
3/17
CMV Promoter ........ 1-595
mWasabi GFP ........ 613-1320
Multiple Cloning Sites ........ 1321-1414
SV40 poly(A) Signal ........ 1560-1610
Ampicillin Resistance Gene ........ 2238-3098
pUC Origin ........ 3247-3889
Sequencing Primer:
mWasabi-F(ABP-FP-WASFPRM) and SV40pA-R
pmWasabi-NT (ABP-FP-WNNCS)
Multiple Cloning Sites
601 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC
661 ATGAAGATCA AGCTGAAGAT GGAGGGCAAC GTGAATGGCC ACGCCTTCGT GATCGAGGGC
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 ACCGTTTACG AGATCGCCGT GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAGT V Y E I A V A R N S T D G M D E L Y K
1321 TCCGGACTCA GATCTCGAGT CGGATCCTCG AATTCGGTAC CCTCTAGACA AGCTTGCGGC CGCTAA
S G L R S R V G S S N S V P S R Q A C G R
BglII XhoI BamH1 EcoRI KpnI HindIII NotI
N
-
8/8/2019 Fluorescent Protein Fusion Expression System
4/17
CMV Promoter ........ 1-589
Multiple Cloning Sites ........ 591-646
mTFP1 TFP ........ 653-1363
SV40 poly(A) Signal ........ 1529-1579
Ampicillin Resistance Gene ........ 2207-3067
pUC Origin ........ 3216-3858
Sequencing Primer:CMV-F and mTFP1-R(ABP-FP-TFPRPRM)
Multiple Cloning Sites
pmTFP1-CT (ABP-FP-TCNCS)
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA
601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC CTCGAGGCCA CCATGGTGAG
661 CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC ATGAAGATCA AGCTGAAGAT
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 CATCGAGATC CTGAACCACG ACAAGGACTA CAACAAGGTG ACCGTTTACG AGAGCGCCGT
1321 GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG TAA
C
-
8/8/2019 Fluorescent Protein Fusion Expression System
5/17
601 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC
661 ATGAAGATCA AGCTGAAGAT GGAGGGCAAC GTGAATGGCC ACGCCTTCGT GATCGAGGGC
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 ACCGTTTACG AGAGCGCCGT GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG
1321 TCCGGACTCA GATCTCGAGT CGGATCCTCG AATTCGGTAC CCTCTAGACA AGCTTGCGGC
1381 CGCTAA
CMV Promoter ........ 1-595
mTFP1 TFP ........ 613-1320
Multiple Cloning Sites ........ 1321-1414
SV40 poly(A) Signal ........ 1560-1610
Ampicillin Resistance Gene ........ 2238-3098
pUC Origin ........ 3247-3889
Sequencing Primer:mTFP1-F(ABP-FP-TFPFPRM) and SV40pA-R
Multiple Cloning Sites
pmTFP1-NT (ABP-FP-TNNCS)
N
-
8/8/2019 Fluorescent Protein Fusion Expression System
6/17
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA
601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC CTCGAGGCCA CCATGGTGAG
661 CAAGGGCGAG GAGGATAACA TGGCCCCTCT CCCAGCAACC CACGATTTAC ACATCTCCGG
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 CGTGTTCCGC AAGGTGGAAC TCAAGCACAC CAAGACTGAG CTCAACTTCA AGCAGTGGCA
1321 GAAGGCATTC CAGGACATCG CCGGCATGGA CGAGCTGTAC AAGTAA
CMV Promoter ........ 1-589
Multiple Cloning Sites ........ 591-646
LanRFP ........ 653-1366
SV40 poly(A) Signal ........ 1532-1582
Ampicillin Resistance Gene ........ 2210-3070
pUC Origin ........ 3219-3861
Sequencing Primer:CMV-F and LanRFP-R(ABP-FP-RFPRPRM)
Multiple Cloning Sites
pLanRFP-CT (ABP-FP-RCNCS)
C
-
8/8/2019 Fluorescent Protein Fusion Expression System
7/17
601 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCCCTCT CCCAGCAACC
661 CACGATTTAC ACATCTCCGG CTCAATCAAT GGACATGAGT TTGACTTGGA AGGCAGTGGC
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 CTCAACTTCA AGCAGTGGCA GAAGGCATTC CAGGACATCG CCGGCATGGA CGAGCTGTAC
1321 AAGAGATCTC GAGTCGGATC CTCGAATTCG GTACCCTCTA GACAAGCTTG CGGCCGCTAA
CMV Promoter ........ 1-595
LanRFP ........ 613-1323
Multiple Cloning Sites ........ 1324-1377
SV40 poly(A) Signal ........ 1554-1604
Ampicillin Resistance Gene ........ 2232-3092
pUC Origin ........ 3241-3883
Sequencing Primer:LanRFP-F(ABP-FP-RFPFPRM) and SV40pA-R
Multiple Cloning Sites
pLanRFP-NT (ABP-FP-RNNCS)
N
-
8/8/2019 Fluorescent Protein Fusion Expression System
8/17
-
8/8/2019 Fluorescent Protein Fusion Expression System
9/17
661 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCTCTCT CCCAGCGACA
721 CATGAGTTAC ACATCTTTGG CTCCTTCAAC GGTGTGGACT TTGACATGGT GGGTCGTGGC
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1321AACTTCAAGG AGTGGCAAAA GGCCTTTACC GATGTGATGG GCATGGACGA GCTGTACAAG
1381 AGATCTCGAG TCGGATCCTC GAATTCGGTA CCCTCTAGAC AAGCTTGCGG CCGCTAA
CMV Promoter ........ 1-595
LanYFP ........ 613-1323
Multiple Cloning Sites ........ 1324-1377
SV40 poly(A) Signal ........ 1554-1604
Ampicillin Resistance Gene ........ 2232-3092
pUC Origin ........ 3241-3883
Sequencing Primer:LanYFP-F(ABP-FP-YFPFPRM) and SV40pA-R
Multiple Cloning Sites
pLanYFP-NT (ABP-FP-YNNCS)
N
-
8/8/2019 Fluorescent Protein Fusion Expression System
10/17
CMV Promoter ........ 1-589
Multiple Cloning Sites ........ 591-646
mWasabi GFP ........ 653-1363
SV40 poly(A) Signal ........ 1529-1579
PGK Promoter ........ 1657-2159
Puromycin Resistance Gene ........ 2242-2841
SV40 poly(A) signal ........ 2965-3191
Ampicillin Resistance Gene ........ 3841-4701
pUC Origin ........ 4850-5492
Sequencing Primer:
CMV-F and mWasabi-R(ABP-FP-WASRPRM)
pmWasabi-CT-PuroR (ABP-FP-WCNCSP)
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA
601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC CTCGAGGCCA CCATGGTGAG
661 CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC ATGAAGATCA AGCTGAAGAT
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 CATCGAGATC CTGAACCACG ACAAGGACTA CAACAAGGTG ACCGTTTACG AGATCGCCGT
1321 GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG TAA
Multiple Cloning Sites
C
-
8/8/2019 Fluorescent Protein Fusion Expression System
11/17
CMV Promoter ........ 1-595
mWasabi GFP ........ 613-1320
Multiple Cloning Sites ........ 1321-1414
SV40 poly(A) Signal ........ 1560-1610
PGK Promoter ........ 1688-2190Puromycin Resistance Gene ........ 2273-2872
SV40 poly(A) signal ........ 2996-3222
Ampicillin Resistance Gene ........ 3872-4732
pUC Origin ........ 4881-5523
Sequencing Primer:
mWasabi-F(ABP-FP-WASFPRM)
pmWasabi-NT-PuroR (ABP-FP-WNNCSP)
Multiple Cloning Sites
601 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC
661 ATGAAGATCA AGCTGAAGAT GGAGGGCAAC GTGAATGGCC ACGCCTTCGT GATCGAGGGC
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1261 ACCGTTTACG AGATCGCCGT GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG
T V Y E I A V A R N S T D G M D E L Y K
1321 TCCGGACTCA GATCTCGAGT CGGATCCTCG AATTCGGTAC CCTCTAGACA AGCTTGCGGC CGCTAA
S G L R S R V G S S N S V P S R Q A C G R
BglII XhoI BamH1 EcoRI KpnI HindIII NotI
N
-
8/8/2019 Fluorescent Protein Fusion Expression System
12/17
CMV Promoter ........ 1-589
Multiple Cloning Sites ........ 591-646
mTFP1 TFP ........ 653-1363
SV40 poly(A) Signal ........ 1394-1620
PGK Promoter ........ 1621-2123
Puromycin Resistance Gene ........ 2206-2805
SV40 poly(A) signal ........ 2929-3155
Ampicillin Resistance Gene ........ 3560-4420
pUC Origin ........ 4575-5194
Sequencing Primer:
CMV-F and mTFP1-R(ABP-FP-TFPRPRM)
pmTFP1-CT-PuroR (ABP-FP-TCNCSP )
Multiple Cloning Sites
587 ATCCGCTAGCTCTAGACTGGATCCTCGAATTCGGTACCTCAAGCTTGCGGCCGCCTCGAGNheI XbaI BamHI EcoRI KpnI HindIII NotI XhoI
647 GCCACCATGGTGAGCAAGGGCGAGGAGACCACAATGGGCGTAATCAAGCCCGACATGAAG
707 ATCAAGCTGAAGATGGAGGGCAACGTGAATGGCCACGCCTTCGTGATCGAGGGCGAGGGC
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1247 TTTGTGGACCACCGCATCGAGATCCTGAACCACGACAAGGACTACAACAAGGTGACCGTT
1307 TACGAGAGCGCCGTGGCCCGCAACTCCACCGACGGCATGGACGAGCTGTACAAGTAA
-
8/8/2019 Fluorescent Protein Fusion Expression System
13/17
CMV Promoter ........ 1-595
mTFP1 TFP ........ 613-1320
Multiple Cloning Sites ........ 1321-1414
SV40 poly(A) Signal ........ 1560-1610
PGK Promoter ........ 1688-2190
Puromycin Resistance Gene ........ 2273-2872
SV40 poly(A) signal ........ 2996-3222
Ampicillin Resistance Gene ........ 3872-4732
pUC Origin ........ 4881-5523
Sequencing Primer:
mTFP1-F(ABP-FP-TFPFPRM)
Multiple Cloning Sites
pmTFP1-NT-PuroR (ABP-FP-TNNCSP)
601 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC
661 ATGAAGATCA AGCTGAAGAT GGAGGGCAAC GTGAATGGCC ACGCCTTCGT GATCGAGGGC
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 ACCGTTTACG AGAGCGCCGT GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG
1321 TCCGGACTCA GATCTCGAGT CGGATCCTCG AATTCGGTAC CCTCTAGACA AGCTTGCGGC
1381 CGCTAA
N
-
8/8/2019 Fluorescent Protein Fusion Expression System
14/17
CMV Promoter ........ 1-589
Multiple Cloning Sites ........ 591-646
LanRFP........ 653-1363
SV40 poly(A) Signal ........ 1532-1582
PGK Promoter ........ 1660-2162
Puromycin Resistance Gene ........ 2245-2844
SV40 poly(A) signal ........ 2968-3194
Ampicillin Resistance Gene ........ 3844-4704
pUC Origin ........ 4853-5495
Sequencing Primer:
CMV-F and LanRFP-R(ABP-FP-RFPRPRM)
pLanRFP-CT-PuroR (ABP-FP-RCNCSP)
Multiple Cloning Sites
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA
601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC CTCGAGGCCA CCATGGTGAG
661 CAAGGGCGAG GAGGATAACA TGGCCCCTCT CCCAGCAACC CACGATTTAC ACATCTCCGG
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 CGTGTTCCGC AAGGTGGAAC TCAAGCACAC CAAGACTGAG CTCAACTTCA AGCAGTGGCA
1321 GAAGGCATTC CAGGACATCG CCGGCATGGA CGAGCTGTAC AAGTAA
C
-
8/8/2019 Fluorescent Protein Fusion Expression System
15/17
CMV Promoter ........ 1-595
LanRFP ........ 613-1323
Multiple Cloning Sites ........ 1324-1377
SV40 poly(A) Signal ........ 1554-1604
PGK Promoter ........ 1682-2184
Puromycin Resistance Gene ........ 2267-2866
SV40 poly(A) signal ........ 2990-3216
Ampicillin Resistance Gene ........ 3866-4726
pUC Origin ........ 4875-5517
Sequencing Primer:
LanRFP-F(ABP-FP-RFPFPRM)
Multiple Cloning Sites
pLanRFP-NT-PuroR (ABP-FP-RNNCSP)
601 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCCCTCT CCCAGCAACC
661 CACGATTTAC ACATCTCCGG CTCAATCAAT GGACATGAGT TTGACTTGGA AGGCAGTGGC
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 CTCAACTTCA AGCAGTGGCA GAAGGCATTC CAGGACATCG CCGGCATGGA CGAGCTGTAC
1321 AAGAGATCTC GAGTCGGATC CTCGAATTCG GTACCCTCTA GACAAGCTTG CGGCCGCTAA
N
-
8/8/2019 Fluorescent Protein Fusion Expression System
16/17
CMV Promoter ........ 1-589
Multiple Cloning Sites ........ 591-646
LanYFP ........ 653-1363
SV40 poly(A) Signal ........ 1529-1579
PGK Promoter ........ 1657-2159
Puromycin Resistance Gene ........ 2242-2841
SV40 poly(A) signal ........ 2965-3191
Ampicillin Resistance Gene ........ 3841-4701
pUC Origin ........ 4850-5492
Sequencing Primer:
CMV-F and LanYFP-R(ABP-FP-YFPRPRM)
pLanYFP-CT-PuroR (ABP-FP-YCNCSP)
Multiple Cloning Sites
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA
601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC CTCGAGGCCA CCATGGTGAG
661 CAAGGGCGAG GAGGATAACA TGGCCTCTCT CCCAGCGACA CATGAGTTAC ACATCTTTGG
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1261 GTTCCGTAAG ACGGAGCTCA AGCACTCCAA GACCGAGCTC AACTTCAAGG AGTGGCAAAA
1321 GGCCTTTACC GATGTGATGG GCATGGACGA GCTGTACAAG TAA
C
-
8/8/2019 Fluorescent Protein Fusion Expression System
17/17
CMV Promoter ........ 1-595
LanYFP ........ 613-1323
Multiple Cloning Sites ........ 1324-1377
SV40 poly(A) Signal ........ 1554-1604
PGK Promoter ........ 1682-2184
Puromycin Resistance Gene ........ 2267-2866
SV40 poly(A) signal ........ 2990-3216
Ampicillin Resistance Gene ........ 3866-4726
pUC Origin ........ 4875-5517
Sequencing Primer:
LanYFP-F(ABP-FP-YFPFPRM)
Multiple Cloning Sites
pLanYFP-NT-PuroR (ABP-FP-YNNCSP)
661 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCTCTCT CCCAGCGACA
721 CATGAGTTAC ACATCTTTGG CTCCTTCAAC GGTGTGGACT TTGACATGGT GGGTCGTGGC
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
1321AACTTCAAGG AGTGGCAAAA GGCCTTTACC GATGTGATGG GCATGGACGA GCTGTACAAG
1381 AGATCTCGAG TCGGATCCTC GAATTCGGTA CCCTCTAGAC AAGCTTGCGG CCGCTAA
N