Figure. S1.1. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of sodium chloride at 22.5oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S1.1
Strains of L. monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637SK600
M39503ASK754
SK2642
SK2508N.a.N.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A58
0nm o
f cry
sta
l vio
let
0.0
0.1
0.2
0.3
0.4
0.5% NaCl (Ctr.)2.0% NaCl5.0% NaCl7.0% NaCl
Serotype 4bSerotype 1/2a
Figure. S1.2. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBTE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of sodium chloride at 30oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S1.2
L. monocytogenes strains
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637SK600
M39503ASK754
SK2642
SK2508N.a.N.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A5
80nm
of
crys
tal v
iole
t
0.0
0.2
0.4
0.6
0.8
1.0
1.2
0.5% NaCl (Ctr.)2.0% NaCl5.0% NaCl7.0% NaCl
Serotype 4bSerotype 1/2a
Figure. S1.3. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of sodium chloride at 37oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S1.3
L. monocytogenes strains
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637SK600
M39503ASK754
SK2642
SK2508N.a.N.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A5
80
nm
of
crys
tal v
iole
t
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
0.5% NaCl (Ctr.)2.0% NaCl5.0% NaCl7.0% NaCl
Serotype 4bSerotype 1/2a
Figure. S2.1. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of glucose at 22.5oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S2.1
Strains of L. monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637SK600
M39503ASK754
SK2642
SK2508N.a.N.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A58
0nm
of
crys
tal v
iole
t
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
Glucose 0.25% (Control)Glucose 1.0%Glucose 5.0%Glucose 10.0%
Serotype 4bSerotype 1/2a
Figure. S2.2. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of glucose at 30oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S2.2
Strains of L. monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637SK600
M39503ASK754
SK2642
SK2508N.a.N.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A58
0nm
of
crys
tal v
iole
t
0.0
0.2
0.4
0.6
0.8
Glucose 0.25% (Control)Glucose 1%Glucose 5%Glucose 10%
Serotype 1/2a Serotype 4b
Figure. S2.3. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of glucose at 37oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S2.3
Strains of L. monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637SK600
M39503ASK754
SK2642
SK2508N.a.N.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A58
0nm
of
crys
tal v
iole
t
0.0
0.2
0.4
0.6
0.8
1.0
1.2
Glucose 0.25% (Control)Glucose 1%Glucose 5%Glucose 10%
Serotype 1/2a Serotype 4b
Figure. S3.1. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of ethanol at 22.5oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S3.1
Strains of Listeria monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637
SK600
M39503ASK754
SK2642
SK2508N.a.N
.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A5
80nm
of c
ryst
al v
iole
t
0.00
0.05
0.10
0.15
0.20
0.25
0% Ethanol (Control)1.0% Ethanol2.0% Ethanol3.0% Ethanol5.0% Ethanol
Serotype 4bSerotype 1/2a
Figure. S3.2. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of ethanol at 30oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S3.2
Strains of Listeria monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637
SK600
M39503ASK754
SK2642
SK2508N.a.N
.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A5
80
nm
of
crys
tal v
iole
t
0.0
0.1
0.2
0.3
0.4
0% Ethanol (Control)1.0% Ethanol2.0% Ethanol3.0% Ethanol5.0% Ethanol
Serotype 4bSerotype 1/2a
Figure S3.3. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of ethanol at 37oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S3.3
Strains of Listeria monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637
SK600
M39503ASK754
SK2642
SK2508N.a.N
.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A5
80nm
of
dest
ain
ed c
ryst
al v
iole
t
0.0
0.2
0.4
0.6
0.8
1.0
0% Ethanol1.0% Ethanol2.0% Ethanol3.0% Ethanol5.0% Ethanol
Serotype 4bSerotype 1/2a
Figure S4.1. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing 1.0% of glucose, or 2% of sodium chloride, or the combination of both at 22.5oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S4.1
Strains of Listeria monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637
SK600
M39503ASK754
SK2642
SK2508N.a.N
.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A5
80
nm o
f des
tain
ed c
ryst
al v
iole
t
0.0
0.2
0.4
0.6
0.8
1.0
1.2
Control (TSBYE)TSBYEgluc1%
TSBYENaCl2%
TSBYEgluc1%, NaCl2%
Serotype 4bSerotype 1/2a
Figure S4.2. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing 1.0% of glucose, or 2% of sodium chloride, or the combination of both at 30oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S4.2
Strains of Listeria monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637
SK600
M39503ASK754
SK2642
SK2508N.a.N
.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A58
0nm
of d
esta
ined
cry
stal
vio
let
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
Control (TSBYE)TSBYEgluc1%
TSBYENaCl2%
TSBYEgluc1%, NaCl2%
Serotype 4bSerotype 1/2a
Figure S4.3. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing 1.0% of glucose, or 2% of sodium chloride, or the combination of both at 37oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S4.3
Strains of Listeria monocytogenes
RM3373
RM3834
RM3835
RM4527
RM3023
RM 3316
RM 3349
RM 3354
RM4543
RM4561
SK1387SK90
SK1637
SK600
M39503ASK754
SK2642
SK2508N.a.N
.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM 4515
RM3817
A58
0nm
of d
est
ain
ed c
ryst
al v
iole
t
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
Control (TSBYE)TSBYEgluc1%
TSBYENaCl2%
TSBYEgluc1%, NaCl2%
Serotype 4bSerotype 1/2a
Listeria monocytogenes strains
RM3373
RM3834
RM3835
RM4527
RM3023
RM3316
RM3349
RM3354
RM4543
RM4561
SK1387SK90
SK1637
SK600
M39503ASK754
SK2642
SK2508N.a.N
.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM4515
RM3817
Gro
wth
rat
e (h
-1)
0.0
0.2
0.4
0.6
0.8
Control (TSBYE)TSBYEgluc1%
TSBYENaCl2%
TSBYEgluc1%+NaCl2%
Serotype 4bSerotype 1/2a
Figure S5.1. Maximum growth rate (h-1) of L. monocytogenes in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) supplemented with extra glucose (1.0%), or sodium chloride (2.0%), or both at 22.5oC. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S5.1
Listeria monocytogenes strains
RM3373
RM3834
RM3835
RM4527
RM3023
RM3316
RM3349
RM3354
RM4543
RM4561
SK1387SK90
SK1637
SK600
M39503ASK754
SK2642
SK2508N.a.N
.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM4515
RM3817
Gro
wth
rat
e (h
-1)
0.0
0.2
0.4
0.6
0.8
1.0
1.2
Control (TSBYE)TSBYEgluc1%
TSBYENaCl2%
TSBYEgluc1%+NaCl2%
Serotype 4bSerotype 1/2a
Figure S5.2. Maximum growth rate (h-1) of L. monocytogenes in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) supplemented with extra glucose (1.0%), or sodium chloride (2.0%), or both at 30oC. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S5.2
Listeria monocytogenes strains
RM3373
RM3834
RM3835
RM4527
RM3023
RM3316
RM3349
RM3354
RM4543
RM4561
SK1387SK90
SK1637
SK600
M39503ASK754
SK2642
SK2508N.a.N
.
SK1450
SK14032140
SK1495
SK1277
M35402A
M33027A
SK1463
RM 2387
RM2992
RM2998
RM4503
RM4504
RM4573
RM3013
RM 3302
RM4515
RM3817
Gro
wth
rat
e (h
-1)
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
Control (TSBYE)TSBYEgluc1%
TSBYENaCl2%
TSBYEgluc1%+NaCl2%
Serotype 4bSerotype 1/2a
Figure S5.3. Maximum growth rate (h-1) of L. monocytogenes in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) supplemented with extra glucose (1.0%), or sodium chloride (2.0%), or both at 37oC. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).
FIG. S5.3