Download - Fetal Pig Dissection!
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Fetal Pig Dissection!
• Work in Groups of 3-4• Get out lab books and lab packets!
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Fetal Pig Dissection – Day 1Getting Started!
Obtain the following materials:1 fetal pig per group
1 large dissection pan2 pieces of string - ~25-30 inches long
2 good scalpelsScissors
2 BLUNT ProbesForceps (tweezers)
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Initial Prep
• Open up the plastic bag – – KEEP the outer bag AND the rubber band!– Cut open the inner bag over the sink. Empty
pig and contents into sink. THROW AWAY inner bag into the GREEN trash
• Rinse off pig well. Towel dry and place in a dissection tray
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External Observations• Look for the umbilical cord
– Try to find the umbilical arteries/veins that connected its blood supply to mom via the placenta
• External nares (remember what those are?)
• Tongue • Boy or Girl?
– Look under the tail. • If all you see it an anus (hole)
then a SMOOTH swollen sac of skin – you have found the SCROTUM and you have a boy!
• If you see an anus (hole) plus an obvious folded area of skin. You have found the GENITAL PAPILLA and you have girl!
Scrotum
Anus
Genital Papilla
Anus
BOY!
GIRL!!!
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Securing your Pig to the tray
• Use the 2 pieces of string to secure your pig to the dissecting tray
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Incision Time1. Take a scalpel – make a
SUPERFICIAL midsaggital incision from chin to umbilical cord
2. Then use scissors to cut along either side of the umbilical cord to create a “flap”
3. & 4. Use scissors to make 2 lateral cuts. Avoid the staples on one side of the pig– Also be careful not to
demolish the DIAPHRAGM at the base of the rib cage!
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Incision Time3. & 4. Use scissors
to make 2 lateral cuts. Avoid the staples on one side of the pig– Also be
careful not to demolish the DIAPHRAGM at the base of the rib cage!
Diaphragm
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Separate Skin from rib cage• Using your scapel – separate the skin of the
chest area off of the rib cage.– You will have to cut through muscle and
connective tissue to do this– It’s like skinning raw chicken!
• Using scissors – cut of each half of the rib cage. You should end up with 2 large chunks of ribs in your tray
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Diaphragm• Use scissors to separate
the diaphragm from the floor of the rib cage.
• You should be left with one big “flap” of Diaphragm that hangs over the liver and stomach (see d page 409 of lab book)
Diaphragm
Diaphragm
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Exposing Larynx and Trachea – pg. 409• Using scissors – cut off any remaining pericardial
sac that might be covering the heart.– Place any pieces in your tray for later disposal
• Using a SHARP scalpel – remove all muscle and tissue overlying the larynx and trachea
Larynx
Trachea
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Neck Blood Vessels – pg. 418 • Using a BLUNT PROBE – carefully probe on either
side of the trachea until you expose– 1 PINK common carotid artery on each side– 2 BLUE jugular veins on each side
• These are the internal and external jugular veins
Internal Jugular Vein
Common Carotid Artery
External Jugular Vein
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Heart• Using a combination
of scalpel and scissors:– Cut off the
pericardial sac that surrounds the heart
– Be careful not to cut off any important blood vessels!
• Also not the texture and position of the RIGHT AND LEFT LUNGS.
Superior Vena Cava
Right LungLeft Lung
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Cardiovascular System:Superior Vena Cava, Pulmonary Trunk & Aortic Arch
Superior Vena Cava
Right Lung Left Lung
Aortic ArchPulmonary Trunk
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What now? Locate all Respiratory & Digestive System organs listed on Page 13 of your lab
packet!• Use pg. 404 & pg. 409 in your lab books for
help
• Note: do NOT find the urinary organs yet – we will do this later.
• When your group thinks it has found everything – call me over so I can review it with you!
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Ready to clean up?• Cut off strings – throw away in GREEN trash.• Throw any PIG TISSUE WASTE in the RED trash
• Rinse pig under water and clean both external and internal areas.
• Spray pig with cavicide (in white spray bottle) inside and out!
• Put pig in your saved plastic bag and secure with rubber band.
• Write the pig’s name (or group name) on the bag in permanent marker.– Place bagged pig in the tray.
• Spray down your table with cavicide or simple green!
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Respiratory System
• Make sure you can locate:– External nares– Larynx– Trachea– Diaphragm– lungs
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Digestive System• Be sure you can locate:
– Tongue– Esophagus– Stomach– Liver– Gallbladder– Small intestine– Large Intestine– Pancreas– Mesentery– Rectum
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Digestive System
Liver
Gallbladder
Small intestine
Large intestine
Mesentery
Rectum
Note: the following are not shown, but you need to know where they are:Tongue, esophagus, stomach, pancreas
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Fetal Pig Dissection – Day 2
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Urinary System• Do NOT cut out the
intestines to dissect the urinary system!
• Push the intestines to one side and use a scalpel or blunt probe to remove the connective tissue around each kidney and ureter
• Note the ALLANTOIC BLADDER
Right Kidney
Left Kidney
Right UreterLeft Ureter
Allantoic Bladder
Rectum
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Reproductive System - MALE
Allantoic Bladder
testes
Penis
Spermatic Cord
EpididymisEpididymis
Spermatic Cord
testes
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Reproductive System - FEMALE
Allantoic Bladder
Ovary
Ovary
Uterine Horn
Uterine Horn
Uterine Body
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Cardiovascular System – Heart & Spleen
heart
Spleen
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Cardiovascular System – page 418– 1 PINK common carotid artery on each side– 2 BLUE jugular veins on each side
• These are the internal and external jugular veins
Internal Jugular Vein
Common Carotid Artery
External Jugular Vein
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Cardiovascular System:Superior Vena Cava, Pulmonary Trunk & Aortic Arch
Superior Vena Cava
Right Lung Left Lung
Aortic ArchPulmonary Trunk
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Cardiovascular System:Inferior Vena Cava & Abdominal Aorta
Inferior Vena Cava
Small Intestine
Mesentery
Abdominal Aorta
Rectum
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Ready to clean up?• Cut off strings – throw away in GREEN trash.• Throw any PIG TISSUE WASTE in the RED trash
• Rinse pig under water and clean both external and internal areas.
• Spray pig with cavicide (in white spray bottle) inside and out!
• Put pig in your saved plastic bag and secure with rubber band.
• Write the pig’s name (or group name) on the bag in permanent marker.– Place bagged pig in the tray.
• Spray down your table with cavicide or simple green!