C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Claus Svendsen & 65 othersUKRI-CEH, Wallingford, United Kingdom
Exposure Assessment and Modelling,
NanoFASE– Intentions, Achievements and Outcomes
EU H2020
(2015-19)
EU FP7 NMP
(2010-14)
NERC ENI3 (‘11-15)
(UK NERC/Defra & US EPA)
EU FP7 NMP
(2013-17)
NOTA BENE – Some slides highlight scientists who presented related papers at ICEEN. Collaborators are recognized! Visit our online library.
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
London (UK)
Thames (UK)catchment
3) Object-oriented multimedia fate modelsdynamically connecting “Environmental cells”
Air
W
ater
SMPPhase
Freephase
Sedimentphase
SolidPhase
Soil
Pore waterPhase
X
Y
2) Environmental„cell“reactors
1) ENM enabledProduct value chains& release pathways
ENM Synthesis
ENM Prod. Manu.
Distribution
Use phase
Waste mgt.
GIS layers providing “cell” specific:• Human population & Industry• Elevation & river network• Vegetation, soil type & landuse• Environmental chemistryRecycling
Conceptual workflow for a framework to deliver dynamic multimedia fate prediction both in a generalised model environment and GIS enabled mode.
Focus on the “real product” and “environmentally realistic/relevant forms.
Exposure assessment in the environment:
How much is released and where does it go?
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Exposure assessment in the environment:
How much is released and where does it go?
London (UK)
Thames (UK)catchment
3) Object-oriented multimedia fate modelsdynamically connecting “Environmental cells”
Air
W
ater
SMPPhase
Freephase
Sedimentphase
SolidPhase
Soil
Pore waterPhase
X
Y
2) Environmental„cell“reactors
1) ENM enabledProduct value chains& release pathways
ENM Synthesis
ENM Prod. Manu.
Distribution
Use phase
Waste mgt.
GIS layers providing “cell” specific:• Human population & Industry• Elevation & river network• Vegetation, soil type & landuse• Environmental chemistryRecycling
Conceptual workflow for a framework to deliver dynamic multimedia fate prediction both in a generalised model environment and GIS enabled mode.
Focus on the “real product” and “environmentally realistic/relevant forms.
Local
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Exposure assessment in the environment:
How much is released and where does it go?
London (UK)
Thames (UK)catchment
3) Object-oriented multimedia fate modelsdynamically connecting “Environmental cells”
Air
W
ater
SMPPhase
Freephase
Sedimentphase
SolidPhase
Soil
Pore waterPhase
X
Y
2) Environmental„cell“reactors
1) ENM enabledProduct value chains& release pathways
ENM Synthesis
ENM Prod. Manu.
Distribution
Use phase
Waste mgt.
GIS layers providing “cell” specific:• Human population & Industry• Elevation & river network• Vegetation, soil type & landuse• Environmental chemistryRecycling
Conceptual workflow for a framework to deliver dynamic multimedia fate prediction both in a generalised model environment and GIS enabled mode.
Focus on the “real product” and “environmentally realistic/relevant forms.
LocalSimplebox
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Exposure assessment in the environment:
How much is released and where does it go?
London (UK)
Thames (UK)catchment
3) Object-oriented multimedia fate modelsdynamically connecting “Environmental cells”
Air
W
ater
SMPPhase
Freephase
Sedimentphase
SolidPhase
Soil
Pore waterPhase
X
Y
2) Environmental„cell“reactors
1) ENM enabledProduct value chains& release pathways
ENM Synthesis
ENM Prod. Manu.
Distribution
Use phase
Waste mgt.
GIS layers providing “cell” specific:• Human population & Industry• Elevation & river network• Vegetation, soil type & landuse• Environmental chemistryRecycling
Conceptual workflow for a framework to deliver dynamic multimedia fate prediction both in a generalised model environment and GIS enabled mode.
Focus on the “real product” and “environmentally realistic/relevant forms.
LocalSimplebox
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Putting the models together and comparing
Towards validating nanomaterial PECs from SimpleBox4nano using the NanoFASE-WSO spatiotemporal multimedia fate model
X
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Putting the models together and comparing
Towards validating nanomaterial PECs from SimpleBox4nano using the NanoFASE-WSO spatiotemporal multimedia fate model
SimpleBox4nano NanoFASE Water-Soil-Organism
Screening level Higher tier
Steady state conditions Time explicit
Regional to continental scale Gridded: 5x5 km
Background, regional concentrations Local concentrations
X
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure assessment (of Nano) in the environment:
How much is released, and where does what go?
The NanoFASE Approach
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure assessment (of Nano) in the environment:
How much is released, and where does what go?
The NanoFASE Approach
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Charting nano releases and forms from products
• Case Study Pathway Analysis to guide experiments
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Nano Product value chain and release paths (WP4)
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Nano Product value chain and release paths (WP4)
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Nano Product value chain and release paths (WP4)
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure assessment (of Nano) in the environment:
How much is released, and where does what go?
Waste Water Management
?
?
?
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Investigating forms leaving end of life stage (WP5)
Investigate fate and transformation of ENM in major managed waste facilities:
Effluent to rivers
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure assessment (of Nano) in the environment:
How much is released, and where does what go?
Waste Water Treatment Plant
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure assessment (of Nano) in the environment:
How much is released, and where does what go?
Waste Water Treatment Plant – Ag and CuO NPs
Gogos, A et al (2017) ES:nano
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure assessment (of Nano) in the environment:
How much is released, and where does what go?
Flows of nano-Ag and distributions among the forms released during its life cycle. All flows are described in tons/year in the European Union with the means of the probability distributions. Adams, V et al 2018 Environmental Pollution, 243, pp 17-27
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure assessment (of Nano) in the environment:
How much is released, and where does what go?
0-95%
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Exposure assessment (of Nano) in the environment:
Modelling air phase distributions?
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Exposure assessment (of Nano) in the environment:
Modelling air phase distributions?
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure assessment (of Nano) in the environment:
How much is released, and where does what go?
Waste Water95% Sludge5% Effluent
Waste Water Treatment Plant
0-95%
Kamil Nešetřil
TUL, Czech
Republic
TULSoil and waterremediation
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
NanoFASE Soil modelling module
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
NanoFASE Soil modelling module
Soil layer, 𝑙 = 1
Soil layer, 𝑙 = 2
Soil layer, 𝑙 = 3
precipitation and evapotranspiration
percolation free NPs
bioturbation attached NPs
erosionattached NPs
bio-uptake
NM sources
transformations
Sam HarrisonUKRI-CEH, UK
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
without rain
Top
Mid
dle
Botto
m
0
2
4
6
8
mg
Ag
/Kg
dry
so
il
day
7 14 21 28
day
7 14 21 28
NP mobility in Soil vs Earthworm driven bioturbation
Baccaro, M. et al, Environmental Pollution, 2019
x ray computed tomography
With worms
Without worms
Marta BaccaroWU, Netherlands
Consequences for leaching and for run-off
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Accumulation kinetics in different biota
Conceptual model
Nico van den Brink, and Marta Baccaro, WU, Netherlands
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Earthworm uptake (ions vs particulate)
14%
day
mg
Ag
/we
t w
eig
ht
Kg
0
4
8
12
16
Ag-particulate prediction
7 14 21 28 35 42 49 56
Total Ag+ prediction
17%
0
4
8
12
16
7 14 21 28 35 42 49 56
day
mg
Ag
/we
t w
eig
ht
Kg
41.8%
as particulate form
Ag-NP exposure AgNO3 exposure
Ag2S-NP exposure
10
x le
ss w
he
n a
ged
Marta BaccaroWU, Netherlands
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Kinetics of uptake of Ag and Ag2S-NPs by wheat in different soils
Tracking Uptake In Organisms (Pristine Vs Aged NM)
The HHHHHHHHHHH
LUFA2.2Standard media
issue!
pH6.5 2%OM
pH6.5 4%OM
pH4.5 15%OM
Elma Lahive
AmaiaEtxabeGreen
Only 2.5 x less when aged
UKRI-CEH, UK
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Accumulation kinetics in different biota
Conceptual modelDetailed study of:Snails;
Mealworms;
Fish;
Nathanial Clark & Richard Handy Uni. of Plymouth, UK
Patricia SilvaUni. of Aveiro.Portugal
Zahra KhodaparastUni. of Aveiro,Portugal
Nico van den Brink, WU, Netherlands
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure assessment (of Nano) in the environment:
How much is released, and where does what go?
Waste Water95% Sludge5% Effluent
5%
Waste Water Treatment Plant
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Assessing NM behaviour in Surface Waters and Sediments
Cover fate in realistic natural water chemistries
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Assessing NM behaviour in Surface Waters and Sediments
Cover fate in realistic natural water chemistries
Model, Test or Measure?
Measure(input to “simple” models)
New tests and models
Current test guidelines
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Assessing NM behaviour in Surface Waters and Sediments
Cover fate in realistic natural water chemistries
Model, Test or Measure?
Measure(input to “simple” models)
New tests and models
Current test guidelines
Inferred from stability underOECD TG 318?Just new GD?
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Assessing NM behaviour in Surface Waters and Sediments
Cover fate in realistic natural water chemistries
Model, Test or Measure?
Measure(input to “simple” models)
New tests and models
Current test guidelines
Inferred from stability underOECD TG 318?Just new GD?
Helene Walch, Uni of Vienna Experimental work
Modelling from:Serge Stoll &
Marianne Seijo
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Putting the models together and comparing
Towards validating nanomaterial PECs from SimpleBox4nano using the NanoFASE-WSO
spatiotemporal multimedia fate model
SimpleBox4nano NanoFASE Water-Soil-Organism
Screening level Higher tier
Steady state conditions Time explicit
Regional to continental scale Gridded: 5x5 km
Background, regional concentrations Local concentrations
Steve Lofts, UKRI-CEH, UK
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Putting the model together and comparing
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Putting the model together and comparing
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Putting the model together and comparing
Meesters, J.A.J. 2019. "A Model Sensitivity Analysis to Determine the Most Important Physicochemical Properties Driving Environmental Fate and Exposure of Engineered Nanoparticles." ES nano 10.1039/C9EN00117D
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Implications for ERA considerations, approaches and tools
How are we planning to handle, share and store the data?
The HHHHHHHHHHH
Environmental fate• Very different - rules if and where there may be possible Nano Exposures
=> should inform what Nano-form(s) are “exposure relevant”• Long time scales => Current standard hazard tests may not be “worst case”
=> Pre-aging of test media?
Biodegradation/ accumulation/ biomagnification• Tested exposure forms must be the exposure relevant ones• Form (size and speciation) of internalised material ideally identified
Technical / Analytical needs + Test Guidance (match question with precision)• New analytical and testing techniques needed (kept simple and repeatable)• Move from “Solute based” to “kinetic” tests
ICPMS tospICPMS
New analytics for organic NMs
X-ray based for speciation
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
N KC
Exposure - Nanomaterial fate in the environment:
How are we are handling, storing, and sharing the data?
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Exposure - Nanomaterial fate in the environment:
How are we are handling, storing, and sharing the data?
The HHHHHHHHHHH
Existingontologies
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Existingontologies
Julian Gallego, UGOT, Sweden
Exposure - Nanomaterial fate in the environment:
How are we are handling, storing, and sharing the data?
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure - Nanomaterial fate in the environment:
In what format will we deliver it to you all?
www.NanoFASE.eu (EU H2020 Proj. 646002)
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure - Nanomaterial fate in the environment:
In what format will we deliver it to you all?
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
How will we deliver it to you all?
How are we planning to handle, share and store the data?
The HHHHHHHHHHH
1. The new knowledge in Papers and Presentations:http://www.nanofase.eu/documents
2. Accessible Online resources and tiered Exposure Assessment Framework:
3. Fantastically well trained people: The (NanoFASE) “Young Nano Scientists”
4. Efforts made to translate our New Knowledge into International Standards:
ICPMS tospICPMS
New analytics for organic NMs
X-ray based for speciation
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure - Nanomaterial fate in the environment:
In what format will we deliver it to you all?
www.NanoFASE.eu (EU H2020 Proj. 646002)
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
NanoFASE “Clickable Framework” modules
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
NanoFASE Exposure Assessment Frameworke.g. WP9 - Accumulation kinetics in different biota
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
NanoFASE Exposure Assessment Frameworke.g. WP9 - Accumulation kinetics in different biota
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
NanoFASE Soil modelling module
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
NanoFASE Soil modelling module
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
NanoFASE Soil modelling module
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
NanoFASE Soil modelling module
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure - Nanomaterial fate in the environment:
In what format will we deliver it to you all?FOCUS ON:
“Reactors” and relevant
ENMs (Focus on relevant
materials at each RA step)
DELIVER:
Functional fate groups
a tool to understand and
reduce complexity
Exposure assessment
framework catalogue of
models, parameters and
methods
Multimedia fate models
simplified to feed regulatory
models (SimpleBox 4 Nano)
DATA + => =>N KC
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Putting it all together for exposure relevant NPs
The HHHHHHHHHHH
Mesocosm kinetic tests (Long term, Low dose, High complexity)• Aquatic and terrestrial• Include most species from earlier single species tests• Performed in Aveiro (Portugal) Summer 2018• Will validate the single species experiments• Results to be expected early 2019.
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
Putting it all together for exposure relevant NPs
The HHHHHHHHHHH
Mesocosm kinetic tests (Long term, Low dose, High complexity)• Aquatic and terrestrial• Include most species from earlier single species tests• Performed in Aveiro (Portugal) Summer 2018• Will validate the single species experiments• Results to be expected early 2019.
“NanoFASE THE MOVIE!” is out – search “NanoFASE and Mesocosm”
The Movie!
C. Svendsen - NanoFASE
ICEENN Sept 2nd 2019
The HHHHHHHHHHH
Exposure - Nanomaterial fate in the environment:
Thank you! Onwards and upwards!