![Page 1: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/1.jpg)
Eukaryotic Intron LossTobias Mourier & Daniel C. Jeffares
![Page 2: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/2.jpg)
Introns
Pre-mRNA
DNA
mRNA
![Page 3: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/3.jpg)
Intron evolution
Bacteria -introns
Archaea -introns
Eukaryota +introns
Introns early/Introns late
![Page 4: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/4.jpg)
Intron evolution
![Page 5: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/5.jpg)
Intron evolution
ATGAAGCGATGT
Met Lys Arg Cys
Intronfase 0
Intronfase 1
Intronfase 2
“Exon Theory of Genes”
![Page 6: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/6.jpg)
Eukaryotic intron loss
Fink GR, Cell 49, 5 (1987):
Introns i Saccharomyces cerevisiae ligger oftest i 5’ (starten) af genet
Introns er tabt gennem rekombination med revers transkriberet cDNA
![Page 7: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/7.jpg)
Eukaryotic intron loss
mRNA
cDNA
Revers transkription
![Page 8: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/8.jpg)
Eukaryotic intron loss
mRNA
cDNA
![Page 9: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/9.jpg)
Eukaryotic intron loss
mRNA
cDNA
Probability of cDNA coverage
5’ Position 3’
![Page 10: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/10.jpg)
Eukaryotic intron loss
mRNA
cDNA
Probability of cDNA coverage
5’ Position 3’
![Page 11: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/11.jpg)
Eukaryotic intron lossMammals
Homo sapiensRattus norvegicusMus musculusVertebrates
Takifugu rubripesDanio rerioInvertebrates
Caenorhabditis elegansAnopheles gambiaeDrosophila melanogasterPlants
Arabidopsis thalianaOryza sativa japonica
Fungi
Neurospora crassaSchizosaccharomyces pombeSaccharomyces cerevisiaeEncephalitozoon cuniculi(Magnaporthe grisae)Alveolates
Plasmodium falciparumPlasmodium yoelii yoeliiSlime mold
Dictyostelium discoideum (chr. II)Red algae
Guillardia theta nucleomorph
~250.000 gener ~900.000 introns
![Page 12: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/12.jpg)
Eukaryotic intron loss
A
B
Relative Intron Position = A/B
![Page 13: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/13.jpg)
12
34
56
78
910
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
Homo sapiens (8.4) Rattus norvegicus (8.3)
Mus musculus (7.6) C. elegans (5.2) A. thaliana (4.2)Oryza sativa (3.2)
Takifugu rubripes (3.1) Danio rerio (3.0) Anopheles gambiae (2.8) D. melanogaster (2.7) Magnaporthe grisea (1.9) Neurospora crassa (1.7)Plasmodium falciparum (1.3)
Dictyostelium discoideum II (1.2) Plasmodium yoelii yoelii (1.1)Schizosaccharomyces pombe (0.95)
Saccharomyces cerevisiae (0.045)Guillardia theta nucleomorph (0.035)
Encephalitozoon cuniculi (0.0075)
Relative intron position
5’ 3’
Fract
ion o
f in
tron
s
![Page 14: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/14.jpg)
12
34
56
78
910
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
Relative intron position
5’ 3’
Fract
ion o
f in
tron
s
Intron erhvervelseeller
Intron tab
![Page 15: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/15.jpg)
0
0.1
0.2
0.3
0.4
0.5
0.6
0 1 2 3 4 5 6 7 8 9
Intron/gene ratio
Average relative intron position
![Page 16: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/16.jpg)
5’ Intron erhvervelse
3’ Intron tab
Insertions bias?Positions-afhængig selektion?
![Page 17: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/17.jpg)
Intron erhvervelse Intron tab
![Page 18: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/18.jpg)
Intron erhvervelse Intron tab
![Page 19: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/19.jpg)
Intron erhvervelse Intron tab
![Page 20: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/20.jpg)
Eukaryotic intron lossMammalsHomo sapiensRattus norvegicusMus musculusVertebratesTakifugu rubripesDanio rerioInvertebratesCaenorhabditis elegansAnopheles gambiaeDrosophila melanogasterPlantsArabidopsis thalianaOryza sativa japonica
FungiNeurospora crassaSchizosaccharomyces pombeSaccharomyces cerevisiaeEncephalitozoon cuniculi(Magnaporthe grisae)AlveolatesPlasmodium falciparumPlasmodium yoelii yoeliiSlime moldDictyostelium discoideum (chr. II)Red algaeGuillardia theta nucleomorph
Sidste fælles stamform?
![Page 21: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/21.jpg)
Johnson PJ, Proc Natl Acad Sci USA 99, 3358 (2002)
Dacks JB, Doolittle WF, Cell 107, 419 (2001)
SSU rRNA
![Page 22: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/22.jpg)
Stechmann A, Cavalier-Smith T, Science 297, 89 (2002)
Gene fusion
![Page 23: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/23.jpg)
Baldauf SL, Science 300, 1703 (2003) Konsensus (molekylære & strukturelle data)
![Page 24: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/24.jpg)
Få introns i encellede eukaryoter er en afledt karakter
Den sidste fælles stamform (sidste fælles eukaryot?) var intron-rig
Eukaryotic intron loss
![Page 25: Eukaryotic Intron Loss Tobias Mourier & Daniel C. Jeffares](https://reader034.vdocuments.us/reader034/viewer/2022052603/56649c815503460f94938d7d/html5/thumbnails/25.jpg)