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Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL
Reconstruction in a Rat Model
by Robert H. Brophy, David Kovacevic, Carl W. Imhauser, Mark Stasiak, Asheesh Bedi, Alice J.S. Fox, Xiang-Hua Deng, and Scott A. Rodeo
J Bone Joint Surg AmVolume 93(4):381-393
February 16, 2011
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 2: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/2.jpg)
Example of mounted external fixator on specimen with Keith needle in place.
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 3: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/3.jpg)
Example of animal connected to loading device.
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 4: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/4.jpg)
Example of negative control histology (mouse anti-rat ED1– microphage stain; original magnification, ×20).
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 5: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/5.jpg)
ED1 staining (mouse anti-rat ED1-macrophage stain) showing inflammatory macrophages in the tissue of a rat from the immobilized group killed at two weeks (original magnification, ×20).
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 6: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/6.jpg)
Factor VIII staining (rabbit anti-human factor VIII, DAKO) showing endothelial cells in the tissue of a rat from the immobilized group killed at four weeks (original magnification, ×20).
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 7: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/7.jpg)
Procollagen staining (Santa Cruz Biotechnology) revealing collagen synthesis in the tissue of a rat from the immobilized group killed at four weeks (original magnification, ×20).
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 8: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/8.jpg)
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 9: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/9.jpg)
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 10: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/10.jpg)
Graph showing the mean daily loading curve (and standard deviation) of the rats that underwent daily loading for two weeks or four weeks.
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 11: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/11.jpg)
Graph showing the mean stiffness data (and standard deviation) from isolated bone-tendon-bone specimens.
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.
![Page 12: Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL Reconstruction in a Rat Model by Robert H](https://reader036.vdocuments.us/reader036/viewer/2022062504/5a4d1b6b7f8b9ab0599b3538/html5/thumbnails/12.jpg)
Graph showing the mean load-to-failure data (and standard deviation) from isolated bone-tendon-bone specimens.
Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393
©2011 by The Journal of Bone and Joint Surgery, Inc.