Dr.AmaniYousefOwaidah AssistantProfessor
PersonalData
Nationality|Saudi
Department|ClinicalLaboratorySciences
OfficialUoDEmail|[email protected]
OfficePhoneNo.3331347
LanguageProficiency
Language Read Write SpeakArabic Advanced Proficient AdvancedEnglish Advanced Advanced AdvancedAcademicQualifications(Beginningwiththemostrecent)
Date AcademicDegree PlaceofIssue AddressMay13,2014 PhD UniversityofBristol Bristol,UnitedKingdomOct13,2009 MScwithMeritin
TransfusionandTransplantationSciences
UniversityofBristol Bristol,UnitedKingdom
Sep8,2004 BScMedicalLaboratorySciences
KingFaisalUniversity Dammam,SaudiArabia
PhD,MasterorFellowshipResearchTitle:(AcademicHonorsorDistinctions)
PhD CellularandMolecularMedicine(StemCellBiologyandCartilagetissueengineering)Master MScwithMeritinTransfusionandTransplantationSciencesProfessionalRecord:(Beginningwiththemostrecent)
JobRank PlaceandAddressofWork DateAssistantProfessor Universityof
DammamDammam,SaudiArabia
September2014-present
ResearchScientist KingFahdSpecialistHospital
Dammam,SaudiArabia
August2015-July2016
SeniorTechnologist(BloodBank)
KingFahdMilitaryMedicalComplex
Dhahran,SaudiArabia
April2007-April2008
JuniorTechnologist(BloodBank)
KingFahdMilitaryMedicalComplex
Dhahran,SaudiArabia
December2004-April2007
ScientificAchievements
PublishedRefereedScientificResearches(InChronologicalOrderBeginningwiththeMostRecent)# NameofInvestigator(s) ResearchTitle PublisherandDateofPublication1 AmaniOwaidahandWael
Kafienah• Cartilage Tissue
Engineering Using Embryonic Stem Cells, Reference Module in Biomedical Sciences.
Elsevier/August24,2016doi:10.1016/B978-0-12-801238-3.99808-8
ContributiontoScientificConferencesandSymposia# ConferenceTitle PlaceandDateoftheConference ExtentofContribution1 SaudiSocietyof
TransfusionmedicineJeddah,SaudiArabiaApril2016
Speaker
2 1stSaudiImmunohematologygroupmeeting
Dammam,SaudiArabiaDecember2015
SpeakerandModerator
3 AdvancesinMolecularBiology
Dammam,SaudiArabiaOctober2014
Speaker
4 StemCellSymposium Cardiff,UnitedKingdomJanuary2014
Speaker
5 InternationalCartilageRepairSociety(ICRS)11thcongress
Izmir,TurkeySeptember2013
Speaker
6 2ndupdateinLaboratoryMedicineMeeting
Dammam,SaudiArabiaApril2013
Speaker
7 2ndSouthWestRegionalRegenerativeMedicineMeeting
Bristol,UnitedKingdomSeptember2012
Speaker
8 InternationalCartilageRepairSociety(ICRS)10thcongress
Montreal,CanadaMay,2012
Speaker
ScientificAwards
• Certificate of excellence by the Saudi Society of Genome and Molecular Oncology (SSGMO) December2013.
• Distinguished Saudi scholars in a ceremony held at the Royal Embassy of Saudi Arabia by HRH PriceMohammedbinNawafAl-Saud(SaudiAmbassadortotheUnitedKingdom)inSeptember2012.
MembershipofScientificandProfessionalSocietiesandOrganizations
• ChairpersonofRedcellImmunohematologygroupoftheSaudiImmunohematologyDiscussiongroup
TeachingActivities
UndergraduateCourseCoordination# CourseTitleandCode Coordination Undergrad. From to1 ClinicalBloodBanking Coursecoordinator 4thyear Sep18-2016 Jan-26,2017StudentAcademicSupervisionandMentoring# Level NumberofStudents From to1 4thyear 1student Sep18,2016 June15,2017PersonalKeyCompetenciesandSkills:(Computer,Informationtechnology,technical,etc.)
• Cell culture and cryo-storage - Generation of human induced pluripotent stem cells (hiPSC) and bonemarrowmesenchymalstemcells.Dailymaintenancethroughtheobservationofcellhealth(visualizationofcells with light microscope and culture media assessment) and then culture of cell lines (feeding,division/expansionandcryostorage).
• Characterizationofpluripotentstemcellandmesenchymalstemcells:-AmajorstageinmyPhDstudies
the characterization of the cell lines through quantitative PCR, immunofluorescence staining,immunohistochemistryandflowcytometry.
• Stem cell differentiation studies – After the successful maintenance of induced pluripotent stem cell
cultures,thecoreofmyPhDwastodesigndifferentiationprotocoltoinducechondrogenicdifferentiationin the human induced pluripotent stem cells. This required extensive understanding of embryonicdevelopmentandcellsignallingpathways.
• 3DTissueengineering–Thiswasimportanttoinvestigatetheabilityofthedifferentiatedhumaninduced
pluripotent stem cells chondroprogenitors to generate a 3D cartilage construct suitable for cell-basedtherapies. The Stem Cell Biology group in the University of Bristol, using mesenchymal stem cells,polyglycolicacidscaffoldandrecombinantgrowthfactors,developedthetissueengineeringprotocol.ForthehiPSC,myPhDwastoinvestigatethefactorsthatinduceitschondrogenicdifferentiation.
• ELISA and colorimetric assay use and result processing - This was important in the measurement of
cartilageextracellularmatrixcomponentssuchastypeIandtypeIIcollagensandsulphatedproteoglycans.
• Histology and immunohistochemistry – These techniqueswere used to analyze the cellularmorphologyand the quality of the 3D tissue engineered cartilage using a wide range of stains. During my PhD Iexperiencedinthedevelopmentandoptimizationofimmunohistochemistrystains.
• Dataanalysis–ThroughoutmyPhDdataanalysishasbeen important.DuringmyrecentPhDstudies the
statisticalpackagePRISM(v5.0c)wasusedtoinvestigatewithin/betweengrouptrends.
• Cell signaling experimentation –Daily experimentation at theUniversity of Bristol was the treatment ofhiPSCwithvarious factors toactivatesignalingpathways involved in theself-renewalanddifferentiation.ThisincludedtheTGFβ,BMP,FGF,andActivinsignalingpathways.
• Blood transfusion and blood banking skills - During my work at King FahdMilitaryMedical Complex in
Dhahran, I experienced all routine and specialized blood banking tests that included ABO +RH forwardgrouping, reversegrouping,Antibodyscreeningand identification,directand indirectantihumanglobulintest, Compatibility testing, resolving ABO discrepancies, acid elution, pre-warm technique, salinereplacementat4oC,coldagglutinins,TypespecificindirectantihumanglobulintestTSIAT,Neutralizationbypooledplasmaandantigentyping).
• Donorregistry,selectionandphlebotomy–Iinstalledacomputerizedprogrammeforthedonorregistryat
KFMMC.Along-sideMrJosephRobinson(Bloodbanksupervisor)tookdecisionroleindonorselection.
• Bloodcomponentpreparationandstorage-duringmytimeatKFMMC,bloodcomponentpreparationandstoragewasanimportanttaskinmyjobdescription.
• Generationandmaintenanceoflabrecordsandstatistics.Adheretostatutoryhealthandsafety
requirements.
LastUpdate
5/12/2016