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DNA
Incompatibility groups:IncIncF-iIncF-iiIncIIncNIncQIncPIncW
DNA
DNAT-DNADNA
T-DNA
.
DNA
IS:bacterial insertion sequencesIS
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DNAmobile DNAKbIS
recombinase/transposase transposition reactions)IS2 , IS3 , IS4 , IS5 , Is1, IS3o, Is66,
IS91, IS110, IS200/IS605,IS256,IS630,IS982,IS1380,ISAs1,ISL3
ISDNA
IS2IS
DNA
cis-acting partition sites
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IncW , IncN, IncQ-2, IncQ-1,IncP, IncI,IncF-2,IncF-1
K12
FRIS
FRIncF-IR1162RSF1010IncQRP4IncPColIbIncIPKM101IncNR388IncW
FDNA:CDTConjugative DNA Transfer
surface & entry exclusion. F-pilus assembly& retraction. . DNA
DNA:CDTConjugative DNA Transferstable mating pair formation
DNAsingle stranded DNA:ssDNAnic sitenic
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siteOrigin of Transfer OriT
DNAduplex DNA: dsDNAssDNA T-DNAssDNA
dsDNA
F oriT500bp sbmA-sbmC,sbyA,ihfB,ihfA,sbiintrinsic bendsdirect
repeats:DRsA-tractsindirect repeats:IRs
nic siteDNAsense DNAihfAsbyA
sbi ,ihfA ,sbyA
TraMsbmA ,sbmB ,sbmCIHFihfBsbyAsbmCTraMsbyA
sbmAsbmBTraMihfBsbmCsbmB
IHFihfAWWWCAANNNNTTRW=A/TR=A/GA/TIHFihfAAAATAGAGTGTTAbpsbyA
TraYGA(T/G)ATraY
TraYTraYIR
relaxosomeoriTDNARelaxase
DNA
T-DNATraYTraI(Integration Host
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Factor:IHFTraYIHFDNADNADNATraI
TraIssDNADNA
TraYIHF TraI
TraM
FFDNADNA in vivoTraMoriT
sbmAsbmBtraMTraM TraM oriTsbmC
DNATraMDNATraDTraD
TraMoriT DNADNA
TraMTraMoriTTraDTraMoriT DNA
TraMsbmCTraM
TraMTraMsbmAsbmBTraM
TraMsbmCDNATraM
TraMDNAoriT DNA
TraMCDT
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CTraI
TraDT4SS
TraD
TraDTraGRP4VirD4Ti
TraDTraMFTraIoriT DNA
TraDT4SSssDNA
TraI
TraIoriTDNACDT
TraInic siteoriTFIHFTraYDNAIHFTraY
DNA
TraYIHFTraI
NDNACDT
CTraITraMTraMCTraI
TraInic site
TraMTraITraYIHFIHFTraYDNAnic site
DNA DNAoriTTraITraMDNA
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TraMDNA
DNAsbiTraYIHFTraIsbiF oriT DNA
TraYsbiTraY-TraITraITraYsbyAIHFihfAoriT DNA
TraI
TraI
ATPT-DNA-TraI Complex
1-Jacques Mahillon & Michael Chandler. 1998. Insertion Sequences. Microbiology and Molecular biology Reviews. Vol.2, No.3, p.725-774.
2-Danal Miller & Joel F.Schildbach. 2003. Evidence for a Monomeric Intermediate in the Reversible Unfolding of F Factor TraM. The Journal of biological chemistry. Vol.278, No.12,Issue of March 21, p.10400-10407.
3-Athanasia Varsaki, Gabriel Moncalián, Maria del piral, Garcillán Barcia, Constantin Drainas, & Fernando dela Cruz. 2009. Analysis of ColE1 MbeC unveils an Extended Ribbon-Helix-Helix Fammily of Nicking Accessory proteins. Journal of bacteriology. Vol.191, No.5, March, p.1446-1455.
4-Christopher Parker, Eric Becker, Xiadin Zhang, Sarah Jandle, Richard Meyu. 2005. Elements in the Co-evolution of relaxasesand their origins of transfer. Plasmid.53.p.113-18.
5-Vitallya Sayulenko, Evgeniy Sagulenko, Simon Jakubowski, Elena Spudich,& Peter J.christie.2001.VirB7 lipoprotein Is Exocellular and associates with the Aggrobacterium tumefaciens T Pilus. Journal of Bacteriology. June,p.3642-3651.
6-Michael T.Howard, William C.Nelson, & Steven W.Matson.1995.Stepwise Assembly of a Relaxosome at the F Plasmid origin of Transfer. The Journal of Biological Chemistry.Vol.270,No.47,Issue of November 24,p.28381-28386.
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7-Sarah L.Williams & Joel F.schildbach.2007.TraY and Integration Host Factor oriT Binding Sites and F Conjugal Transfer, Sequence Variations,but Not Altered Spacing,Are Tolerated. Journal bacteriology, May.Vol.189,No.10,p.3813-3823.
8-Heather Ragonese, Debi Haisch, Ernesto Villareal, June Hyukchoi & Steuen W.Matson.2007. The F Plasmid-encoded TraM Protein Stimulates relaxosome-mediated Cleavage at oriT through on interaction with TraI. Molecular Microbiology.vol.63,No.4,p.1173-1184.
9-Claire M.Hamilton,Hyewon lee, Pei-lili, David M.Cook, Kevin R.Piper,Sussane Beck, von Bodman, Erich Lanka, Walf Ream & Stephen K.Farrand.2000. TraG fram RP4 and TraG & VirD4 from Ti Plasmids Confer Relaxosome Specificity to the Conjugal Transfer System of pTiC58. Journal of Bcteriology. Vol.182,No.6,p.1541-1548.
10-Tatsuhico Abo & Elichi Ohtsubo.1993. Repression of the tram gene of Plasmid R100 by Its Own Product and Integration Host Factor at one of the Two Promoters. Journal of Bacteriology. Vol.175,No.14,p.4466-4474.
11-J.Ignacio Sastre, Elene Cabenzón, & Fernando dela Cruz.1998. The Carboxyl Terminus of Protein TraD Adds Specificity and Efficiency to F-Plasmid-Conjugative Transfer. Vol.180,No.22,p.6039-6042.
12-Werner Panegrau, Günter Ziegelin, and Erich Lanka.1990. Covalent Association of the traI gene product of Plasmid RP4 with the 5'-Terminal Nucleotide at the Relaxation Nick Site. The Journal of biological chemistry. Vol.265,No.18,Issue of june 25,p.10637-10644.
13-Benjamin Lewin.2005. Genes7.Oxford University Press.