Download - DEPT. of Physiology, Preclinic School of Xinjiang Medical University Renal physiology Lifeng Wang
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DEPT. of Physiology, Preclinic School of Xinjiang Medical University
Renal physiology
Lifeng Wang
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OBJECTIVE
To learn the technique of bladder intubation;
To observe the factors that influence urine formation;
To analyze the mechanisms of how these factors act.
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Principles
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Kidney -- nephron
Renal cortex
Renal medullaRenal pyramid
Renal pelvis
Ureter Renal pyramid
nephron
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Structure of kidney
Nephron
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Processes of Urine formation
Glomerular filtrationTubular reabsorptionTubular secretion
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Factors affecting the three processes of unrine formation
The regulation of glomerular filtration rate(GFR)EFP
glomerular capillary blood pressure glomerular capillary colloid osmotic
pressure bowman’s capsule hydrostatic pressure
renal plasma flowThe position of filtration equilibrium
glomerular capillary filtration coefficientThe condition of filtration membrane
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Factors affecting the three processes of unrine formation
The regulation of tubular reabsorption and secretion Effect of solute concentration in the tubular
fluid and osmotic diuresisOsmotic diuresis
Glomerulotubular balance
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Factors affecting the three processes of unrine formation
Nervous regulationSympathetic nerve fibers(+) →urine formation↓
Renal blood flow↓ Na+ reabsorption ↑Rennin releasing↑
Hormonal regulationADH (anti-diuretic hormone)→urine formation↓
Water reabsorption↑
RAAS(Renin-angiotensin-aldsterone system) →urine formation↓
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EQUIPMENTS AND DRUGSBL-420 biological functional experimental
systemblood pressure transducerarterial cannuladouble concave clip iron-standthree-way tapstimulating electrode……
Jungular anatomy of rabbit
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SAMPLE: rabbitMETHODS AND PROCEDURES
Weigh, anaesthesia and fixing rabbitDosage,Speed and position of injection
Operation Cut open the skin, separate the subcutaneous tissue and muscle to
expose the tracheaEndotracheal intubation: “ ”shape⊥Separate the nerves and the left common carotid artery: Separate the nerves: vagus nerve, Intubation in the left common carotid artery: Connect the arterial cannula with BL-420 biological functional experimental
system Intubation in the urinary bladder or urethra
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ITEMS FOR OBSERVATION
Observes the normal blood pressure and urine volume Injection of Normal saline(15~20ml, 38 )℃stimulate the peripheral side of vagus nerve, and
make the blood pressure fluctuate around 50mmHg for 15~20 seconds
Injection of 25% glucose solution(5ml, 38℃ Injection of 1/10000 noradrenalin (0.3~0.5ml) Injection of furosemide (0.5ml) Injection of pituitrin(3~5 Iu)
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QUESTIONS and PRECAUTIONS
QUESTIONSAnalyze the mechanisms of each result
PRECAUTIONS Injection should be slow during anaesthesia Protect the the marginal vein in the earAvoid injury of nerve and arteryObserve the next item until the effect of
previous one die away.