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Data Blitz
Eric Spivey5 May 2010
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Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions.
Motivation: Use this method to fabricate cell scaffolds for neural tissue engineering applications.
Goal: Fabricate hydrogel microstructures with tunable functional and mechanical properties.
Focused, pulsed laser
Biopolymer Solution
Biopolymer Hydrogel Matrix Microscope Objective
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Hertz Model
RadiusR
Force = F
Displacement = d
• Can calculate Young’s Modulus with F, R and d,• Assume:
Poisson’s ratio is 0.5 and Substrate measured is infinitely thick
• Corrections exist for non-infinite substrates
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Force-indentation
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Dwell time measurements
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Dwell Time Measurements
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Dwell Time Measurements
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Finite Element Modeling
From Dintwa et. al. Granular Matter. 2008
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Implications
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Protein Cantilevers
From Khirpin et. al. Soft Matter. 2010 (in press)
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BSA/GMHA Cantilevers
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Next
• Validate system using PDMS standard – initial attempts with high modulus sample did not work
• Use lower force, lower strain system (low spring constant, large R) to take measurements (reduce high strain systemic error)
• Incorporate high-strain systemic error estimation into current model
• Further explore “protein cantilever” method
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Fabricated Microstructures
5 µm
5 µm
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Other
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Repeatability
Day 3
Day 1
1 mW 2 mW 3 mW 4 mW
80 µm/sec