POSTER SESSION: Molecular Diagnostics, April 12-13, 201718340 Yorba Linda Blvd. Ste 107PMB 427, Yorba Linda, CA 92886
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Valida&on of our HTG EdgeSeq Pla6orm for accurate and sensi&ve biomarker discovery
S.Danilin, G. Bilger, H Firat, E. Schordan, Firalis SAS, Huningue, France
Conclusion
Firalis, leading group in Biomarker discovery and qualifica&on validates its next genera&on gene expression HTG EdgeSeq pla6orm at its site in Huningue, France.
Vast field of exper&se Exper&se from discovery to market State of the art tools
Simple workflow
Sensi&ve chemistry
Minimal sample input HTG EdgeSeq technology
8 patient samples were analyzed using both HTG WTA miRNA panel and qPCR Exiqon miRNA panel V4 (341 miRNA in common).
Results show a good correlation between both methods.
HTG edgeseq offers accurate results
HTG edgeseq offers high reproducibility
Comparison between 4 independant experiments (sets) on the same sample using the miRNA WTA assay
Sets 1,2,3 : HTG (Tucson, USA) with different instruments/days/operators
Set 4 : Firalis, (Huningue, France)
HTG mean by miRNA 0 2 4 6 8 10 12
qPCR
mea
n by
miR
NA
40
35
3
0
25
20
Sample correla&on
Set1/Set2 0.999
Set1/Set3 0.998
Set2/Set3 0.999
Set3/Set4 0.993
Extrac&on free Eliminates biases from RNA
extracition Sensi&vity of next genera&on sequencing
Various panels are available
WTA assay : a study example Project outlines - Validation of miRNAs with predictive value previously identified. - Samples: 15 µl of plasma from 75 patients with negative outcome and 67 patients with positive
outcome. - Screening of miRNAs 24 control with HTG EdgeSeq WTA
Results: - 313 miRNAs are above the threshold in plasma samples. - Predictive value of selected miRNAs confirmed and new miRNAs identified.
! HTG Edgeseq platform available at Firalis offers a new cost effective and reliable tool for accurate and sensitive RNA expression measurement.
! Its low sample input requirements and extraction free process make it an invaluable tool for biomarker discovery.
Contact : [email protected]
Conclusion
2084 miRNAs (miR20 database)
Oncology biomarkers
Whole transcriptome miRNA assay 24 HK
Immuno-‐onco drug response and immuno response
Lymphoma-‐associated genes assay
WATCH: Poster Presentation https://youtu.be/eQBS0jGinwI CONTACT: Sabrina Danilin [email protected]
