Considering a new
approach to treating
Diabetic neuropathy
DISCLOSURES
Bruce Werber DPM FACFAS
Founder and Past President of Amnio Technology 2012-2015
Past President StimStep wireless 2016
Current CEO Anu Life Sciences
Patents
orthopedic plate and screw system 8529608 ndash Terrill et al 2012
acellular placental tissue processing United States Patent 9132156 ndash Werber et al 2015
7 pending patents related to placental tissue 1 pending related to orthopedic biologic implant
Financial relationships
Biotech Products Services and Research (BPSR ndash OTC) COO
Anu LifeSciences llcCEO
AniCell Biotechnologies llc investor and consultantld) amniotic allografts for Animals
There is a single light of science and to brighten it
anywhere is to brighten it everywhere
Isaac Asimov
THE CHALLENGE NON-HEALING WOUNDS AND PAIN
The Problem Diabetic Peripheral Neuropathy66 Type 1 diabetics59 Type 2 diabetics
Non PainfulNot aware
of its
presence
increased
risk of
wounds
Affect almost 50
Of Patients with type
1 and 2
29 Mil people in USA
with Diabetes
90 unaware that they
Have neuropathy
WHO predicts44 of
population by
2030
171 mil in 2000
366 mil in 2030
60-70 have
neuropathy
Painful taking
meds with variety
of adverse events
d
i
a
b
e
ti
c
The Neuropathic ConsequencesMore than 50 develop infected wounds
20 go on to amputationContralateral leg gt50 risk of amputation
5 year mortality after amputation is 68
DSPN cost USA 245Billion year
DFU cost 100k minimum per episode
The Maze of Causation Theories
Dellon et al
fibro-osseous compression sites nerve enlargement and entrapment as diabetic metabolic disease developed
Shimizu et al
Increase type IV collagen and Fibronectin production due to glycation end products Basement
membrane becomes thicker
Dyck et alcompression of the affected No evidence of axonal degeneration
LEEDiabetic nerve enlargement has been demonstrated
ObrosovaCannot replenish free radical scavengers causing slow down of the ATP energy production
Double Crush
Syndrome
Mechanical stiffening of connective tissue affecting
pericytes
Swelling within from extracellular
fluid
Evidence increased diameter on US studies
C-fibers affected
early stages
Authors confirmatory studies ndash Rosenbloom-silverstein 25-26 Tesafye 26
Zhang Lee 242728 Upton-Mccomas 29 Dellon 30
Neuropathic pain is very often refractory to
phamacotherapies along with considerable
unwanted side effects
THE DOWNSIDE OF PHARMACOTHERPYNeuropathic pain is very often refractory to
pharmacotherapies greater than 30 to 50 40 unable to achieve any level of pain relief
along with considerable unwanted side
effects
Remember
Pharmaceuticals are toxins with beneficial
side effects
long-term use of these conventional medications
may lead to significant side effects as well as
abuse 42 4344
pharmaceuticals = high cost to the health system
patients that fail these therapies - suffer
pain and wounds 45
Pharmaceuticals are toxins with beneficial side effects
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
DISCLOSURES
Bruce Werber DPM FACFAS
Founder and Past President of Amnio Technology 2012-2015
Past President StimStep wireless 2016
Current CEO Anu Life Sciences
Patents
orthopedic plate and screw system 8529608 ndash Terrill et al 2012
acellular placental tissue processing United States Patent 9132156 ndash Werber et al 2015
7 pending patents related to placental tissue 1 pending related to orthopedic biologic implant
Financial relationships
Biotech Products Services and Research (BPSR ndash OTC) COO
Anu LifeSciences llcCEO
AniCell Biotechnologies llc investor and consultantld) amniotic allografts for Animals
There is a single light of science and to brighten it
anywhere is to brighten it everywhere
Isaac Asimov
THE CHALLENGE NON-HEALING WOUNDS AND PAIN
The Problem Diabetic Peripheral Neuropathy66 Type 1 diabetics59 Type 2 diabetics
Non PainfulNot aware
of its
presence
increased
risk of
wounds
Affect almost 50
Of Patients with type
1 and 2
29 Mil people in USA
with Diabetes
90 unaware that they
Have neuropathy
WHO predicts44 of
population by
2030
171 mil in 2000
366 mil in 2030
60-70 have
neuropathy
Painful taking
meds with variety
of adverse events
d
i
a
b
e
ti
c
The Neuropathic ConsequencesMore than 50 develop infected wounds
20 go on to amputationContralateral leg gt50 risk of amputation
5 year mortality after amputation is 68
DSPN cost USA 245Billion year
DFU cost 100k minimum per episode
The Maze of Causation Theories
Dellon et al
fibro-osseous compression sites nerve enlargement and entrapment as diabetic metabolic disease developed
Shimizu et al
Increase type IV collagen and Fibronectin production due to glycation end products Basement
membrane becomes thicker
Dyck et alcompression of the affected No evidence of axonal degeneration
LEEDiabetic nerve enlargement has been demonstrated
ObrosovaCannot replenish free radical scavengers causing slow down of the ATP energy production
Double Crush
Syndrome
Mechanical stiffening of connective tissue affecting
pericytes
Swelling within from extracellular
fluid
Evidence increased diameter on US studies
C-fibers affected
early stages
Authors confirmatory studies ndash Rosenbloom-silverstein 25-26 Tesafye 26
Zhang Lee 242728 Upton-Mccomas 29 Dellon 30
Neuropathic pain is very often refractory to
phamacotherapies along with considerable
unwanted side effects
THE DOWNSIDE OF PHARMACOTHERPYNeuropathic pain is very often refractory to
pharmacotherapies greater than 30 to 50 40 unable to achieve any level of pain relief
along with considerable unwanted side
effects
Remember
Pharmaceuticals are toxins with beneficial
side effects
long-term use of these conventional medications
may lead to significant side effects as well as
abuse 42 4344
pharmaceuticals = high cost to the health system
patients that fail these therapies - suffer
pain and wounds 45
Pharmaceuticals are toxins with beneficial side effects
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
THE CHALLENGE NON-HEALING WOUNDS AND PAIN
The Problem Diabetic Peripheral Neuropathy66 Type 1 diabetics59 Type 2 diabetics
Non PainfulNot aware
of its
presence
increased
risk of
wounds
Affect almost 50
Of Patients with type
1 and 2
29 Mil people in USA
with Diabetes
90 unaware that they
Have neuropathy
WHO predicts44 of
population by
2030
171 mil in 2000
366 mil in 2030
60-70 have
neuropathy
Painful taking
meds with variety
of adverse events
d
i
a
b
e
ti
c
The Neuropathic ConsequencesMore than 50 develop infected wounds
20 go on to amputationContralateral leg gt50 risk of amputation
5 year mortality after amputation is 68
DSPN cost USA 245Billion year
DFU cost 100k minimum per episode
The Maze of Causation Theories
Dellon et al
fibro-osseous compression sites nerve enlargement and entrapment as diabetic metabolic disease developed
Shimizu et al
Increase type IV collagen and Fibronectin production due to glycation end products Basement
membrane becomes thicker
Dyck et alcompression of the affected No evidence of axonal degeneration
LEEDiabetic nerve enlargement has been demonstrated
ObrosovaCannot replenish free radical scavengers causing slow down of the ATP energy production
Double Crush
Syndrome
Mechanical stiffening of connective tissue affecting
pericytes
Swelling within from extracellular
fluid
Evidence increased diameter on US studies
C-fibers affected
early stages
Authors confirmatory studies ndash Rosenbloom-silverstein 25-26 Tesafye 26
Zhang Lee 242728 Upton-Mccomas 29 Dellon 30
Neuropathic pain is very often refractory to
phamacotherapies along with considerable
unwanted side effects
THE DOWNSIDE OF PHARMACOTHERPYNeuropathic pain is very often refractory to
pharmacotherapies greater than 30 to 50 40 unable to achieve any level of pain relief
along with considerable unwanted side
effects
Remember
Pharmaceuticals are toxins with beneficial
side effects
long-term use of these conventional medications
may lead to significant side effects as well as
abuse 42 4344
pharmaceuticals = high cost to the health system
patients that fail these therapies - suffer
pain and wounds 45
Pharmaceuticals are toxins with beneficial side effects
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
The Problem Diabetic Peripheral Neuropathy66 Type 1 diabetics59 Type 2 diabetics
Non PainfulNot aware
of its
presence
increased
risk of
wounds
Affect almost 50
Of Patients with type
1 and 2
29 Mil people in USA
with Diabetes
90 unaware that they
Have neuropathy
WHO predicts44 of
population by
2030
171 mil in 2000
366 mil in 2030
60-70 have
neuropathy
Painful taking
meds with variety
of adverse events
d
i
a
b
e
ti
c
The Neuropathic ConsequencesMore than 50 develop infected wounds
20 go on to amputationContralateral leg gt50 risk of amputation
5 year mortality after amputation is 68
DSPN cost USA 245Billion year
DFU cost 100k minimum per episode
The Maze of Causation Theories
Dellon et al
fibro-osseous compression sites nerve enlargement and entrapment as diabetic metabolic disease developed
Shimizu et al
Increase type IV collagen and Fibronectin production due to glycation end products Basement
membrane becomes thicker
Dyck et alcompression of the affected No evidence of axonal degeneration
LEEDiabetic nerve enlargement has been demonstrated
ObrosovaCannot replenish free radical scavengers causing slow down of the ATP energy production
Double Crush
Syndrome
Mechanical stiffening of connective tissue affecting
pericytes
Swelling within from extracellular
fluid
Evidence increased diameter on US studies
C-fibers affected
early stages
Authors confirmatory studies ndash Rosenbloom-silverstein 25-26 Tesafye 26
Zhang Lee 242728 Upton-Mccomas 29 Dellon 30
Neuropathic pain is very often refractory to
phamacotherapies along with considerable
unwanted side effects
THE DOWNSIDE OF PHARMACOTHERPYNeuropathic pain is very often refractory to
pharmacotherapies greater than 30 to 50 40 unable to achieve any level of pain relief
along with considerable unwanted side
effects
Remember
Pharmaceuticals are toxins with beneficial
side effects
long-term use of these conventional medications
may lead to significant side effects as well as
abuse 42 4344
pharmaceuticals = high cost to the health system
patients that fail these therapies - suffer
pain and wounds 45
Pharmaceuticals are toxins with beneficial side effects
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
The Neuropathic ConsequencesMore than 50 develop infected wounds
20 go on to amputationContralateral leg gt50 risk of amputation
5 year mortality after amputation is 68
DSPN cost USA 245Billion year
DFU cost 100k minimum per episode
The Maze of Causation Theories
Dellon et al
fibro-osseous compression sites nerve enlargement and entrapment as diabetic metabolic disease developed
Shimizu et al
Increase type IV collagen and Fibronectin production due to glycation end products Basement
membrane becomes thicker
Dyck et alcompression of the affected No evidence of axonal degeneration
LEEDiabetic nerve enlargement has been demonstrated
ObrosovaCannot replenish free radical scavengers causing slow down of the ATP energy production
Double Crush
Syndrome
Mechanical stiffening of connective tissue affecting
pericytes
Swelling within from extracellular
fluid
Evidence increased diameter on US studies
C-fibers affected
early stages
Authors confirmatory studies ndash Rosenbloom-silverstein 25-26 Tesafye 26
Zhang Lee 242728 Upton-Mccomas 29 Dellon 30
Neuropathic pain is very often refractory to
phamacotherapies along with considerable
unwanted side effects
THE DOWNSIDE OF PHARMACOTHERPYNeuropathic pain is very often refractory to
pharmacotherapies greater than 30 to 50 40 unable to achieve any level of pain relief
along with considerable unwanted side
effects
Remember
Pharmaceuticals are toxins with beneficial
side effects
long-term use of these conventional medications
may lead to significant side effects as well as
abuse 42 4344
pharmaceuticals = high cost to the health system
patients that fail these therapies - suffer
pain and wounds 45
Pharmaceuticals are toxins with beneficial side effects
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
The Maze of Causation Theories
Dellon et al
fibro-osseous compression sites nerve enlargement and entrapment as diabetic metabolic disease developed
Shimizu et al
Increase type IV collagen and Fibronectin production due to glycation end products Basement
membrane becomes thicker
Dyck et alcompression of the affected No evidence of axonal degeneration
LEEDiabetic nerve enlargement has been demonstrated
ObrosovaCannot replenish free radical scavengers causing slow down of the ATP energy production
Double Crush
Syndrome
Mechanical stiffening of connective tissue affecting
pericytes
Swelling within from extracellular
fluid
Evidence increased diameter on US studies
C-fibers affected
early stages
Authors confirmatory studies ndash Rosenbloom-silverstein 25-26 Tesafye 26
Zhang Lee 242728 Upton-Mccomas 29 Dellon 30
Neuropathic pain is very often refractory to
phamacotherapies along with considerable
unwanted side effects
THE DOWNSIDE OF PHARMACOTHERPYNeuropathic pain is very often refractory to
pharmacotherapies greater than 30 to 50 40 unable to achieve any level of pain relief
along with considerable unwanted side
effects
Remember
Pharmaceuticals are toxins with beneficial
side effects
long-term use of these conventional medications
may lead to significant side effects as well as
abuse 42 4344
pharmaceuticals = high cost to the health system
patients that fail these therapies - suffer
pain and wounds 45
Pharmaceuticals are toxins with beneficial side effects
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
Double Crush
Syndrome
Mechanical stiffening of connective tissue affecting
pericytes
Swelling within from extracellular
fluid
Evidence increased diameter on US studies
C-fibers affected
early stages
Authors confirmatory studies ndash Rosenbloom-silverstein 25-26 Tesafye 26
Zhang Lee 242728 Upton-Mccomas 29 Dellon 30
Neuropathic pain is very often refractory to
phamacotherapies along with considerable
unwanted side effects
THE DOWNSIDE OF PHARMACOTHERPYNeuropathic pain is very often refractory to
pharmacotherapies greater than 30 to 50 40 unable to achieve any level of pain relief
along with considerable unwanted side
effects
Remember
Pharmaceuticals are toxins with beneficial
side effects
long-term use of these conventional medications
may lead to significant side effects as well as
abuse 42 4344
pharmaceuticals = high cost to the health system
patients that fail these therapies - suffer
pain and wounds 45
Pharmaceuticals are toxins with beneficial side effects
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
Neuropathic pain is very often refractory to
phamacotherapies along with considerable
unwanted side effects
THE DOWNSIDE OF PHARMACOTHERPYNeuropathic pain is very often refractory to
pharmacotherapies greater than 30 to 50 40 unable to achieve any level of pain relief
along with considerable unwanted side
effects
Remember
Pharmaceuticals are toxins with beneficial
side effects
long-term use of these conventional medications
may lead to significant side effects as well as
abuse 42 4344
pharmaceuticals = high cost to the health system
patients that fail these therapies - suffer
pain and wounds 45
Pharmaceuticals are toxins with beneficial side effects
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
THE DOWNSIDE OF PHARMACOTHERPYNeuropathic pain is very often refractory to
pharmacotherapies greater than 30 to 50 40 unable to achieve any level of pain relief
along with considerable unwanted side
effects
Remember
Pharmaceuticals are toxins with beneficial
side effects
long-term use of these conventional medications
may lead to significant side effects as well as
abuse 42 4344
pharmaceuticals = high cost to the health system
patients that fail these therapies - suffer
pain and wounds 45
Pharmaceuticals are toxins with beneficial side effects
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
A NEW PATHWAY to TREATMENT
And
Neuro-Modulation
A New Paradigm
Biologics
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
VAGUS NERVEmdash controls
everything from blood pressure to
sexual arousalmdashto treat inflammation
associated with PTSD
NERVE STIMULATION conveys a signal to
the spleen where the platelets
receive the signal
This signal ldquoprimesrdquo the platelets
prepping them to form clots if they
encounter a wound anywhere in the body
NEURAL TOURNIQUET reduced bleeding time by 40 percent and
volume of blood loss by 50 percent Within three minutes of
jolting the pigrsquos nerve the researchers measured an increase in
an enzyme associated with clotting at the site of injury
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
A new Treatment alternative for autoimmune disease
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
PERCUTANEOUS WIRELESS
NEUROMODULATION
W I R E L E S S P A I N R E L I E F T M
Neuromodulation a pathway to
improved control and avoiding the
morbidities of long term medications
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
Quell device
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
WE ALREADY USE ELECTRIC BONE
STIMULATION
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
HOW DOES NEUROMODULATION WORKActively stimulating targeted nerve to produce a natural biological response Frequency 10HZ
Meta analysis by
Thalkral RCT by
Koel
RBecker
Injury site + charge
Flip polarity to ndash
Promote healing
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
Blastema - Tissue Regeneration ndash Salamanders
Blastema is the point of regeneration of lost parthellip
Reversing the electrical field creating a shift in polarity allows blastema to
growhellip
if Polarity does not shift then there is no healing
Same in a non healing fracture Dr Robert Becker (bone stimulation upstate NY medical center)
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
PLACENTAL ALLOGRAFT
OPHTHALMIC HEALING
mdash Tandon R Br J Ophthalmol 201195(2)199-204
At
Presentation
Final
VisitAmniotic Membrane
Transplant
Standard Medical
Care
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
Day 0 ndash 47cm Day 63 ndash Closed Margin
Patient LM - Comparative
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
22
Pre injection
painful scar
BedriddenFirst in Man Amniotic Stem Cell Injection Promotes Scar Remodeling and
Healing Processes in Late-Stage Fibrosis
Courtney Hemphill Katherine Stavoe Zain Khalpey
PII S0167-5273(14)00682-2
DOI doi 101016jijcard201404023
International Journal of Cardiology
14 days post injection
no pain
normal turgor
walking without pain
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
Day 0 10mm x 11mm x 2mm
Day 60
Fully resolved
Patient BC (Day 0 vs 60)
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
Design Key for our new Paradigm
02 increase amplitude
(volume) of signaling in
recipients body to produce
appropriate proteinsbull
01 Upregulate
recipients body to
heal itself
Electrical Stimulus
amp
Biologic Stimulusbull
03 ldquostem cell function deteriorates with age driving events we know occur with agingrdquo bull Dr David Scadden -
Harvard Stem Cell Institute
04 ldquonerve cells appear to be one mdashsignal to others that itrsquos time to age ldquoDr Lee Rubin Harvard-Harvard Stem Cell
Institute
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
REVERSE THE PROCESShelliphelliphellipIS IT POSSIBLE
Hamza 68 reports improvement in the vascular function with neuromodulation therapy
causing neurotransmitter release
increased endorphins
inhibiting depolarization of the neurons
Not only can neuropathic pain be inhibited but the possibility of reversing the neuropathy
is potentially possible
Gordon et al 74 in their rat study -- demonstrated that axon regeneration may be
accelerated by brief periods of electrical stimulation
Deogaonkar et al 677576 increases in local capillary blood flow release of
neurotransmitters and cytokines and improved nerve function induced by electric
stimulation to the specific anatomic area 77
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
SNAKE OIL OR THE REAL DEAL
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult
rats
Becker D1 Gary DS Rosenzweig ES Grill WM McDonald JW
Front Hum Neurosci 2015 Oct 199586 doi 103389fnhum201500586 eCollection 2015
Electrical Stimulation Elicits Neural Stem Cells Activation New Perspectives in CNS Repair
Huang Y1 Li Y2 Chen J1 Zhou H1 Tan S1
Exp Neurol 2014 Oct26012-8 doi 101016jexpneurol201209016 Epub 2012 Oct 1
Regeneration of the damaged central nervous system through reprogramming technology basic concepts and
potential application for cell replacement therapy
Matsui T1 Akamatsu W1 Nakamura M2 Okano H3
Int J Neurosci 2015125(10)774-83 doi 103109002074542014971121 Epub 2014 Oct 31
Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the
regeneration of crush injured rat sciatic nerve
Kim YT1 Hei WH Kim S Seo YK Kim SM Jahng JW Lee JH
Up-regulation of MSCrsquos with
Exposure to electrical fields
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
Bringing together Neuromodulation and Regenerative Biologics
Developing the possibility
of Stabilizing or reversing
the neuropathic process
02
Cellular
components
Pericytes-gt
MSC-gt growth
factors
03
Activity
Glucose control
Nutrition
Neuromodulation
Improve blood flow
with
Change in electrical
field polarity
frequency
Voltage and
Amplitude
Placental
Tissue
Messenger
microRNA
exosomes
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
RESULTS WIRELESS STIMULATOR TRIAL
6 patients with painful neuropathy unresponsive to standard of care
treatments
One leg on each patient was treated
Implant wireless stimulator along common peroneal and a second implant
placed along tibial nerve
Each patient used device a minimum 12 hours per day
5 patient reported a 80 reduction in pain at 6 weeks continuing to end of
study at 24 weeks
The implant 13cm long
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
29
Lateral AP
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
IMPLANT PLACEMENT ndash FIBULAR NERVE AKA
COMMON PERONEAL
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
YOU ARE TRAINED TO HEAL YOUR PATIENTS
Yes We need the evidence
But that does not mean you should ignore the possibilitieshelliphellip Allow yourself to think out of the Box
Donrsquot let third parties be the physicians and you the technician
Offer your patients all options covered or nothelliphellip That are safe and appropriate
Thank you for your time
Please feel free to ask your Questions
Bruce Werber DPM FACFAS
602-405-3247
docWerberGmailcom
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip
THANK YOU FOR YOUR TIME
Dr Bruce Werber
All Americans want is the freedom and
security to pursue possibilityhelliphellip