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Cofactors and Coenzymes
• Some enzymes do not need any additional
components to show full activity. • However, others require non-protein molecules
called cofactors to be bound for activity.• Cofactors can be either inorganic (e.g., metal
ions and iron-sulfur clusters) or organic compounds, (e.g., flavin and heme).
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Cofactors• Active enzyme / Holoenzyme:
– Polypeptide portion of enzyme (apoenzyme) – Nonproteineous group (cofactor)
• Cofactors are bound to the enzyme for it to maintain the correct configuration of the active site– Metal ions– Organic compounds– Organometallic compounds
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Organic cofactors can be either:prosthetic groups, which are tightly bound to
an enzyme, or coenzymes, which are released from the enzyme's active site during the reaction.
carbonic anhydrase, with a zinc cofactor bound as part of its active site. These tightly-bound molecules are usually found in the active site and are involved in catalysis.
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For example, flavin and heme cofactors are often
involved in redox reactions.
Most cofactors are not covalently attached to an enzyme, but are very tightly bound. However, organic prosthetic groups can be covalently bound.
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Coenzymes
Coenzymes are small organic molecules that transport chemical groups in an enzymatic reaction.
ORAny of a number of freely diffusing organic
compounds that function as cofactors with enzymes in promoting a variety of metabolic reactions.
Some of these chemicals such as riboflavin, thiamine and folic acid are vitamins, (acquired).
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The chemical groups carried include the hydride ion (H-) carried by NAD or NADP+, the acetyl group carried by coenzyme A, … etc.
Since coenzymes are chemically changed as a consequence of enzyme action, it is useful to consider coenzymes to be a special class of substrates, or second substrates, which are common to many different enzymes.
About 700 enzymes are known to use the coenzyme NADH.
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Coenzymes are usually regenerated and their concentrations maintained at a steady level inside the cell: for example, NADPH is regenerated through the pentose phosphate pathway and S-adenosylmethionine by methionine adenosyltransferase
Coenzymes participate in enzyme-mediated catalysis in stoichiometric (mole-for-mole) amounts and are modified during the reaction.
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Coenzymes• A coenzyme is
required by some enzymes– An organic molecule
bound to the enzyme by weak interactions / Hydrogen bonds
– Most coenzymes carry electrons or small groups
– Many have modified vitamins in their structure
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Nicotinamide Adenine Dinucleotide in Oxidized and Reduced Forms
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NAD+ to NADH Mechanism
• The nicotinamide part of NAD+ accepts a hydride ion (H plus two electrons) from the alcohol to be oxidized
• The alcohol loses a proton ( H+ ) to the solvent
N
CONH2
H
R+
R1CH
HOH N
CONH2
H
R
H
+ R1CH
O
oxred+
Oxidized form Reduced form+H+
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Two Other Adenine Dinucleotide Coenzymes
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