Test Name Test Code Technique Specimen TAT/Reported on
Anti-Cyclic Citrullinated Peptide - Anti CCP
RIM10196Chemiluminescence
Microparticle Immunoassay (CMIA)
3 ml (1.5 ml Minimum) Serum from 1 SST (Gel
Barrier Tube) drawn between 8- 10 a.m. In a
fasting condition
Same Day by 7:00 p.m.
Anti-Nuclear Antibody - ANA, ELISA
RIM10301ELISA (Enzyme Linked
Immuno sorbent Assay)
2 ml. (1 ml. Minimum) Serum from 1 SST (Gel
Barrier Tube).
Next Working Day by 7:00 p.m.
Anti-Nuclear Antibody - ANA, IFA
RIM10187
IFA (Immunofluorescence Assay)
Anti-Neutrophilic Cytoplasmic Antibodies - ANCA
RIM101915th working by 7:00
p.m.
Smooth Muscle Antibody (ASMA), IFA
RIM10251Next Working Day by
7:00 p.m.
ASLO (Antistrepto Lysin -O) RBC10074
Latex Agglutination
Same Day by 7:00 p.m.
CRP (C-Reactive Protein)-Qualitative
RBC10084
Rheumatoid Factor - RF Factor, Qualitative
RSR10008 Slide Agglutination
Rheumatoid Factor -RF Factor, (Quantitative)
RBC10083 Nephelometry
Acetylcholine Receptor Auto antibodies
RIM10242Radio Immunoassay
(RIA5th working day by
7:00 p.m.
Anti-Soluble Liver Antigen (SLA) RIM10305ELISA (Enzyme Linked
Immuno sorbent Assay)4th Working Day by
7:00 p.m.
Anti-Mitochondrial Antibody - AMA, IFA
RIM10218
IFA (Immunofluorescence Assay)
Next Working Day by 7:00 p.m.
LKM Antibody in Dilutions, IFA (Liver Kidney Microsomal Antibody in Dilutions, IFA)
RIM102505th working day by
7:00 p.m.
ds DNA antibody (Reflex to end titer for all positive cases)
RIM10207Next Working Day by
7:00 p.m.Anti -ds DNA Antibody, ELISA RIM10208
ELISA (Enzyme Linked Immuno sorbent Assay)
ENA (Screen)Panel Qualitative (Extractable Nuclear Antigen Screen Panel Qualitative)
RP10030 LIA (LINE Immunoassay)3 ml (1.5 ml. Minimum) Serum from 2 SSTs (Gel
Barrier Tube).
4th Working Day by 7:00 p.m.
HLA B-27, Disease Association PCR SMO10079 PCR
5 - 6 ml EDTA anticoagulated Whole
blood in 2 Lavender top tubes.
3rd working day if received before
1400 hrs.
HLA B-27 (Flowcytometry) SFC10056 Flow Cytometry3 ml. (2 ml. Minimum) Whole Blood in 1 EDTA
(Lavender top) tube
Next Working Day if received before
1400 Hrs
TTG Antibody IgA, Tissue Transglutaminase IgA Antibody
RIM10239ELISA (Enzyme Linked
Immuno sorbent Assay)
2 ml. (1 ml. Minimum) Serum from 1 SST (Gel
Barrier Tube).
Next Working Day by 7:00 p.m.
TEST INFORMATION
Regd. Office & Main Lab .: 3- Factory Road , Adj. Safdarjung Hospital, New Delhi - 29
laboratories
DISCOVERDIAGNOSEDEFEND
Clinical Testing for Autoimmune Disorders
India’s Leading Super Specialized Laboratory Network
Anti CCP (Citrullinated Cyclic Peptide antibodies)
ASMA (Anti-Smooth muscle antibodies) by Immunofluorescence
Rheumatoid Factor Testing
Acetylcholine Receptor Antibodies
HLA B27 (Human Leukocyte antigen)
• Aids in diagnosis of patients with unexplained joint inflammation, especially in Seronegative RF (Rheumatoid Factor)
• Provides differential diagnosis of Rheumatoid arthritis from other forms of joint disease
• Initial Screening test and highly sensitive • Helps to evaluate a person for autoimmune disorders that affect many tissues
and organs throughout the body
• ANCA tests are used to diagnose and monitor inflammatory activity in the primary systemic small vessel vasculitis
• May be useful for monitoring treatment response in patients with WG (systemic or organ-limited disease); increasing titer suggests relapse of disease, while a decreasing titer suggests successful treatment
• Consist of fewer extractable antigens or recombinant antigens that can be detected by solid phase technique
• More specific than IFA as specific Ag can be used to detect specific disease• Potential for quantification
• M2 antibody is the most commonly detected and clinically significant mitochondrial antibody
• Helps in detection of antibodies in patients with PBC (Primary Biliary Cirrhosis)
• Specific marker for Autoimmune Hepatitis Type-1 (50-80%) & PBC (20%)
• Can also be found in Drug induced hepatitis, Alcohol induced liver cirrhosis, Primary Sclerosing cholangitis, Breast & Ovarian Cancer and 2% of apparently healthy persons
• Can be transiently detected in other viral infections including Hepatitis B, C & Infectious Mononucleosis
• Absence of SMA indicates non- autoimmune forms of Chronic Hepatitis
• Approximately 85% of patients with rheumatoid arthritis have detectable RF• It may also be seen in 1-5% of healthy people and in other with medical conditions
like Sjogren’s syndrome and Systemic Lupus Erythematosus (SLE), Tuberculosis, syphilis etc
• Majority of MG patients demonstrate elevated serum levels of acetylcholine receptor (AChR) antibodies
• Myasthenia gravis has been confirmed by the detection of anti-AChR antibody in 85-90% of the patients with generalized disease and in 75% of cases with restricted ocular myasthenia
• Used for the evaluation of seronegative spondyloarthropathies• Aids in diagnosis of Ankylosing Spondylitis & Reiter’s syndrome• Single platform Technology, Dual Laser & 6 color immunofluorescence technique
is used to identify & report percentages of HLA B27 positive cell population with utmost precision
More Than a Decade of Experience in delivering Pathological Solution India’s Leading Super Specialized Laboratory Network
ANA (Antinuclear Antibody) by Immunofluorescence
Anti-Neutrophilic Cytoplasmic Antibodies – ANCA
ELISA: Poly or Monospecific
• Multiplexed immunoassays and uses recombinant antigens• Easy to perform, rapid • Comparing the color intensities• Highly sensitive, SSA/Ro + can be detected• Automation is possible
LIA (Line Blot)
AMA (Anti-Mitochondrial Antibody) by Immunofluorescence
Also available• SLA/LP (Anti-Soluble Liver Antigen/Liver-Pancreas)
• LKM (Liver Kidney Microsomal Antibody)
• Anti-ds DNA
• Anti-TTG (Anti-Tissue Transglutaminase Antibody)
• Immunoglobulins (Quantitative & Qualitative)
• CRP (C-Reactive Protein) Testing
• ASO/ASLO (Anti-Streptolysin O)