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CHI SEQUENCE
MOLECULAR BIOLOGY
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INTRODUCTION
Crossover hotspot instigator(CHI).
Chi sites are referred to as "recombination hot spots“.
Chi sites was originally discovered in bacteriophage
lambda, a virus that infects E. coli, but is now known to
occur about 1000 times in the E. coli genome.
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A Chi site or Chi sequence is a short stretch of DNA in the
genome of a bacterium near which homologous
recombination occur.
Homologous recombination occurs between sequences
that are nearly identical.
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The Chi site is unique to each group of closely related
organisms.
Eg: 5'-GCTGGTGG-3‘(in salmonella).
The Chi sequence serves as a signal to the RecBCD helicase-exonuclease that triggers a major change in the activities of this enzyme.
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CHI SITE MEDIATED HOMOLOGOUS RECOMBINATION BY RecBCD PATHWAYA
STEP 1:
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STEP 2:
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RecBCD : A COMPLEX ENZYME
It has endonuclease subunit(RecBCD) which cuts one DNA strand close to Chi sequence.
And DNA helicase activity (recD subunit).
RecA:
Catalyzes strand exchange.
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ROLE OF CHI SITE
Generating new gene/allelli. E.g: immunoglobulin rearrangement.
Intigration of a specific DNA element.
DNA repair.
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Thank you