Download - Cell membrane and transport
![Page 1: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/1.jpg)
AP Biology
The Cell Membrane
2007-2008
![Page 2: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/2.jpg)
Regents Biology
Phospholipids Phosphate head
hydrophilic Fatty acid tails
hydrophobic Arranged as a bilayer
Fatty acid
Phosphate
Aaaah, one of thosestructure–functionexamples
“repelled by water”
“attracted to water”
![Page 3: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/3.jpg)
Regents Biology
Arranged as a Phospholipid bilayer Serves as a cellular barrier / border
polarhydrophilicheads
nonpolarhydrophobictails
polarhydrophilicheads
H2Osugar
lipids
salt
NH3
impermeable to polar molecules
![Page 4: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/4.jpg)
Regents Biology
How do we know? Even with EM, hard to see membrane “Fuzzy
area”
![Page 5: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/5.jpg)
Regents Biology
How do we know? 1925: two experiments on erythrocytes
(red blood cells)
Hugo Fricke “Membrane thickness 3.3nm” Accurate result, misinterpretation
“membranes are single molecule layers”
Dr. E. Groter and F. Grendel Measured cell SA Measured length of membrane lipids Rough ratio 1:2*
![Page 6: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/6.jpg)
Regents Biology
Davson-Danielli Model 1935 Hugh Davson & James Danielli Phospholipid bilayer sandwiched by
globular protein layer
• “Flanking protein layer” Explained surface tension
• Not supported by experimental evidence
![Page 7: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/7.jpg)
Regents Biology
Cell membrane must be more than lipids…
In 1972, S.J. Singer & G. Nicolson proposed that membrane proteins are inserted into the phospholipid bilayer
It’s like a fluid…It’s like a mosaic…It’s the Fluid Mosaic Model!
![Page 8: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/8.jpg)
Regents Biology
Freeze Fracturing & the Fluid Mosaic Model:
1. Obtain very thin sections of dehydrated tissue (dead) <0.05 µm -ultramicrotome (uses glass knife)
2. Freeze fracture-freeze specimen in liquid N (-100°C)
3. Strike with ultramicrotome blade• lipids don’t freeze as well as proteins = points of weakness!• peels membrane in two, exposes internal structure4. Coat specimen with vaporized metal (gold, platinum)• Low points and high points5. Metal atoms scatter electrons in EM• High points appear Lghter b/c electrons don’t hit film
![Page 9: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/9.jpg)
Regents Biology
Freeze and Fracture
![Page 10: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/10.jpg)
Regents Biology
Coat sample with metal
![Page 11: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/11.jpg)
Regents Biology
Scan with Electron Microscope
![Page 12: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/12.jpg)
Regents Biology
Membrane is a collage of proteins & other molecules embedded in the fluid matrix of the lipid bilayer
Extracellular fluid
Cholesterol
Cytoplasm
Glycolipid
Transmembraneproteins
Filaments ofcytoskeleton
Peripheralprotein
Glycoprotein
Phospholipids
1972, S.J. Singer & G. Nicolson proposed Fluid Mosaic Model
![Page 13: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/13.jpg)
Regents Biology
Membrane carbohydrates Play a key role in cell-cell recognition
ability of a cell to distinguish one cell from another antigens
important in organ & tissue development
basis for rejection of foreign cells by immune system
![Page 14: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/14.jpg)
Regents Biology
Cell membrane defines cell Cell membrane separates living cell from
aqueous environment thin barrier = 8nm* thick 1nm = 1/1,000,000,000m = 1×10−9 m
Controls traffic in & out of the cell allows some substances to cross more
easily than others hydrophobic (nonpolar) vs. hydrophilic (polar)
![Page 15: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/15.jpg)
Regents Biology
Permeability to polar molecules? Membrane becomes semi-permeable via
protein channels specific channels allow specific material
across cell membrane
inside cell
outside cell
sugaraaH2O
saltNH3
![Page 16: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/16.jpg)
Regents Biology
Cell membrane is more than lipids… Transmembrane proteins embedded in
phospholipid bilayer create semi-permeabe channels
lipid bilayermembrane
protein channelsin lipid bilyer membrane
![Page 17: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/17.jpg)
AP Biology 2007-2008
Why areproteins the perfect molecule to build structures in the cell membrane?
![Page 18: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/18.jpg)
Regents Biology
Classes of amino acidsWhat do these amino acids have in common?
nonpolar & hydrophobic
![Page 19: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/19.jpg)
Regents Biology
Classes of amino acidsWhat do these amino acids have in common?
polar & hydrophilic
I like thepolar onesthe best!
![Page 20: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/20.jpg)
Regents Biology
Proteins domains anchor molecule
Within membrane nonpolar amino acids
hydrophobic anchors protein
into membrane
On outer surfaces of membrane in fluid polar amino acids
hydrophilic extend into
extracellular fluid & into cytosol
Polar areasof protein
Nonpolar areas of protein
![Page 21: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/21.jpg)
Regents Biology
NH2
H+
COOH
Cytoplasm
Retinalchromophore
Nonpolar(hydrophobic)a-helices in thecell membrane H+
Porin monomer
b-pleated sheets
Bacterialoutermembrane
proton pump channel in photosynthetic bacteria
aquaporin = water channel in bacteria
function through conformational change = protein changes shape
Examples
H2O
H2O
H+
H+
![Page 22: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/22.jpg)
Regents Biology
Many Functions of Membrane Proteins
Outside
Plasmamembrane
InsideTransporter Cell surface
receptorEnzymeactivity
Cell surface identity marker
Attachment to thecytoskeleton
Cell adhesion
“Antigen”
“Channel”
![Page 23: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/23.jpg)
Regents Biology
Membrane Proteins Proteins determine membrane’s specific functions
cell membrane & organelle membranes each have unique collections of proteins
Classes of membrane proteins: peripheral proteins
loosely bound to surface of membrane ex: cell surface identity marker (antigens)
integral proteins penetrate lipid bilayer, usually across whole membrane transmembrane protein ex: transport proteins
channels, pumps (permeases)
![Page 24: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/24.jpg)
Regents Biology
Questions??
![Page 25: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/25.jpg)
AP Biology
Movement across the Cell Membrane
2007-2008
Play the song, Ms. N!
![Page 26: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/26.jpg)
Regents Biology
Diffusion 2nd Law of Thermodynamics
governs biological systems universe tends towards disorder (entropy)
Diffusion movement from HIGH LOW concentration
![Page 27: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/27.jpg)
Regents Biology
Simple Diffusion Move from HIGH to LOW concentration
“passive transport” no energy needed
diffusion osmosis
movement of water
![Page 28: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/28.jpg)
Regents Biology
Facilitated Diffusion Diffusion through protein channels
channels move specific molecules across cell membrane
no energy needed
“The Bouncer”
open channel = fast transport
facilitated = with help
HIGH
LOW
![Page 29: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/29.jpg)
Regents Biology
Active Transport Cells may need to move molecules against
concentration gradient conformational shape change transports solute
from one side of membrane to other protein “pump” “costs” energy = ATP
“The Doorman”
conformational change
ATP
LOW
HIGH
![Page 30: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/30.jpg)
Regents Biologysymportantiport
Active transport Many models & mechanisms
ATP ATP
![Page 31: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/31.jpg)
Regents Biology
Getting through cell membrane Passive Transport
Simple diffusion diffusion of nonpolar, hydrophobic molecules
lipids HIGH LOW concentration gradient
Facilitated transport diffusion of polar, hydrophilic molecules through a protein channel
HIGH LOW concentration gradient
Active transport diffusion against concentration gradient
LOW HIGH uses a protein pump requires ATP
ATP
![Page 32: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/32.jpg)
Regents Biology
Transport summary
simplediffusion
facilitateddiffusion
activetransport
ATP
![Page 33: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/33.jpg)
Regents Biology
How about large molecules? Moving large molecules into & out of cell
through vesicles & vacuoles endocytosis
phagocytosis = “cellular eating” pinocytosis = “cellular drinking”
exocytosis
exocytosis
![Page 34: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/34.jpg)
Regents Biology
Endocytosis
phagocytosis
pinocytosis
receptor-mediated endocytosis
fuse with lysosome for digestion
non-specificprocess
triggered bymolecular signal
![Page 35: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/35.jpg)
AP Biology
The Special Case of Water
Movement of water across the cell membrane
2007-2008
![Page 36: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/36.jpg)
Regents Biology
Osmosis is just diffusion of water Water is very important to life,
so we talk about water separately Diffusion of water from
HIGH concentration of water to LOW concentration of water across a
semi-permeable membrane
![Page 37: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/37.jpg)
Regents Biology
Concentration of water Direction of osmosis is determined by
comparing total solute concentrations Hypertonic - more solute, less water Hypotonic - less solute, more water Isotonic - equal solute, equal water
hypotonic hypertonic
water
net movement of water
![Page 38: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/38.jpg)
Regents Biologyfreshwater balanced saltwater
Managing water balance Cell survival depends on balancing
water uptake & loss
![Page 39: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/39.jpg)
Regents Biology
Managing water balance Hypotonic
a cell in fresh water high concentration of water around cell
problem: cell gains water, swells & can burst
example: Paramecium ex: water continually enters
Paramecium cell
solution: contractile vacuole pumps water out of cell ATP
plant cells turgid = full cell wall protects from bursting
freshwater
ATP
1
No problem,here
KABOOM!
![Page 40: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/40.jpg)
Regents Biology
Pumping water out Contractile vacuole in Paramecium
ATP
![Page 41: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/41.jpg)
Regents Biology
Managing water balance Hypertonic
a cell in salt water low concentration of water
around cell problem: cell loses water &
can die example: shellfish solution: take up water or
pump out salt plant cells
plasmolysis = wilt can recover
saltwater
2
I willsurvive!
I’m shrinking,I’m shrinking!
![Page 42: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/42.jpg)
Regents Biology
Managing water balance Isotonic
animal cell immersed in mild salt solution
no difference in concentration of water between cell & environment problem: none
no net movement of water flows across membrane equally, in
both directions cell in equilibrium volume of cell is stable
example: blood cells in blood plasma slightly salty IV solution in hospital
balanced
3
I couldbe better…
That’sperfect!
![Page 43: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/43.jpg)
Regents Biology
Aquaporins Water moves rapidly into & out of cells
evidence that there were water channels protein channels allowing flow of water
across cell membrane
1991 | 2003
Peter AgreJohn Hopkins
Roderick MacKinnonRockefeller
![Page 44: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/44.jpg)
Regents Biology
Cell (compared to beaker) hypertonic or hypotonic
Beaker (compared to cell) hypertonic or hypotonic
Which way does the water flow? in or out of cell
.05 M .03 M
Do you understand Osmosis…
![Page 45: Cell membrane and transport](https://reader035.vdocuments.us/reader035/viewer/2022062404/553a25524a79597a748b4a71/html5/thumbnails/45.jpg)
Regents Biology
Any Questions??