![Page 1: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/1.jpg)
Cell damage:Cell damage: necrosis, necrosis, apoptosis.apoptosis. General General
DDeatheath
Ass.prof. Golovata Tatiana
![Page 2: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/2.jpg)
Necrosis (from the Greek. Nekros - Necrosis (from the Greek. Nekros - dead)dead). It’s. It’s deathdeath of cells and tissues in of cells and tissues in living organism. Necrosis may be living organism. Necrosis may be in onein one cell, a group of cells, part of the bodycell, a group of cells, part of the body or or organorgan..
![Page 3: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/3.jpg)
1 - Preneсrosis or paranecrosis2 - Necrobiosis3 - Actually necrosis4 - Autolysis
Stages
![Page 4: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/4.jpg)
Preneсrosis or paranecrosis- changes similar to necrotic, but
reverse
![Page 5: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/5.jpg)
Necrobiosis- profound degenerative changes, in which the prevailing catabolic over
anabolic changes
![Page 6: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/6.jpg)
Actually necrosis –cell death when the time of death can
not be established
![Page 7: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/7.jpg)
Autolysis- decomposition of dead substrate under the influence of hydrolytic
enzymes
![Page 8: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/8.jpg)
Microscopic signs of necrosis. Microscopic signs of necrosis. Changes in the nucleusChanges in the nucleus
1.Kariopiknosis - wrinkling nucleus (Я), caused by condensation of chromatin
![Page 9: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/9.jpg)
Microscopic signs of necrosis. Microscopic signs of necrosis. Changes in the nucleusChanges in the nucleus
2.Karyorhexis – breakdown of nucleusnucleus
![Page 10: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/10.jpg)
Microscopic signs of necrosis. Microscopic signs of necrosis. Changes in the nucleusChanges in the nucleus – kariorhexis in lymph
follicle
nuclei lymph follicle appear as small fragments
![Page 11: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/11.jpg)
Microscopic signs of necrosis. Microscopic signs of necrosis. Changes in the nucleusChanges in the nucleus
3. Kariolysis - total splitting of the nucleus by hydrolytic enzymes
![Page 12: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/12.jpg)
Microscopic signs of necrosis. Microscopic signs of necrosis. Cytoplasm changesCytoplasm changes
1.Plasma-coagulation: protein denaturation and coagulation 2. Plasmorhexis -cytoplasm decomposition 3. Plasmolysis - hydrolytic fusion of cytoplasm
The process ends with a complete dissolution of cells
![Page 13: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/13.jpg)
Microscopic signs of necrosis. Microscopic signs of necrosis. Changes Changes intercellular substanceintercellular substance
In the interstitial substance of the intercellular space depolymerization with glucosamineglycane and saturation with blood plasma proteins develops. As a result interstitial substance becomes swollen and fuses. The collagen and elastic fibers are fused too. Reticular fibers are preserved longer than the other structures. Then they dissociate to lumps.
![Page 14: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/14.jpg)
Classification of necrosis
I. According to the cause:
1) traumatic (caused by chemical or physical factors);2) toxic (toxins of bacteria, chemicals);3) trophoneurotic (in disturbances of nervous trophic)- bed-sore4) allergic (develops in the sensitized organism as hypersensitivity reaction of immediate type);5) vascular (infarction).
![Page 15: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/15.jpg)
Classification of necrosis
II. According to the clinic and morphological forms:1) coagulative (dry) necrosis;2) colliquative (liquefactive) necrosis;3) gangrene (originate from Greek <<gangraina>> fire) necrosis of tissue adjacent to the outer environment:
a) dry b) wet
4) sequestration;5) infarction.
![Page 16: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/16.jpg)
Classification of necrosis
III. According to mechanism of its development it may be direct and indirect.Direct necrosis arise immediate action of causal factor (toxic and traumatic);Indirect (mediate) necrosis may be vascular and trophoneurotic.
![Page 17: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/17.jpg)
CCoagulatioagulativeve necrosi necrosiss
This is the most common type of necrosis which is caused by ischemia, and less often by bacterial and chemical agents. The organs commonly affected are the heart, kidneys and spleen.
![Page 18: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/18.jpg)
CCoagulatioagulativeve necrosi necrosiss
In gross examination the foci of coagulative necrosis in the early stage are pale, firm and slightly swollen. Microscopic change probably results from denaturation of structural and enzymatic proteins
![Page 19: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/19.jpg)
Colliquative necrosis
Colliquative necrosis due to ischemic injury and bacterial infections. Because of hydration and colliquation of tissue by the action of powerful hydrolytic enzymes. The common examples are brain infarct and abscess cavity.
![Page 20: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/20.jpg)
Colliquative necrosis
In gross examination the affected area is soft and swollen. Late a cyst wall is formed. Microscopically, the cystic space contains necrotic cell detritus. Macrophages filled with phagocytosed material.
![Page 21: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/21.jpg)
IInfarctionnfarction
Infarction is vascular necrosis.The causes of infarction are prolonged vascular spasm, thrombosis or embolism .
![Page 22: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/22.jpg)
Sequestration
Sequestration is an area of dead tissue which does not experience autolization and is freely located in the living tissue. It is characteristic for osteomyelitis - purulent inflammation of the bone marrow.
![Page 23: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/23.jpg)
GangreneGangrene
Wet gangrene. Occurs under the action of putrefactive microorganisms. Tissue to swell, become swollen, emit a fetid smell, demarcation zone is not defined.
Wet gangrene occur in the lungs, intestines and uterus.
![Page 24: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/24.jpg)
GangreneGangrene
Dry gangrene begins in the distal part of a limb due to ischemia. The typical example is the dry gangrene in the toes and feet of an old patient due to arteriosclerosis.
![Page 25: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/25.jpg)
Bed-sore
Is a kind of gangrene, death of the tissue under the influence of pressure (sacral area, spinous processes, great trochanter). It is trophoneurotic necrosis in severily ill patients.
![Page 26: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/26.jpg)
Outcome of necrosis
Favorable: 1) organization, replacement by connective tissue
with formation of a scar or a capsule; 2) petrifaction;3) ossification, formation of bone;4) aseptic autolysis. Unfavorable: saprogenic fusion of necrotic tissue followed by
sepsis.
![Page 27: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/27.jpg)
APOPTOSIS
Apoptosis - genetically programmed necrosis unwanted or defective cells in living organism and is aimed at the destruction of cells during embryogenesis and physiological involution: death epithelium of the skin, red and white blood cells.
Biological meaning of apoptosis is the elimination of damaged cells.
Between 50 and 70 billion cells die each day due to apoptosis in the average human adult.
![Page 28: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/28.jpg)
Morphogenesis of apoptosis
These changes include :
- blebbing, cell shrinkage;
- nuclear fragmentation;
- chromatin condensation and chromosomal DNA fragmentation;
- formation of apoptotic bodies;
- phagocytosis of apoptotic bodies by macrophages or parenchymal cells.
![Page 29: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/29.jpg)
In contrast to necrosis, which is a form of traumatic cell death that results from acute cellular injury, apoptosis, in general, confers advantages during an organism's life cycle.
![Page 30: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/30.jpg)
Death
Depending on the cause distinguish natural (physiological) death from old age and wear on the body, violent death from injury or other negative effects on the body ending in death, and from diseases (inviolent)
![Page 31: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/31.jpg)
Stages of dying
Agony Clinical (somatic ) death Biological (molecular) death
![Page 32: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/32.jpg)
Agony
Terminal condition characterized:
Violation of the central nervous system (sopor or coma)
Low blood pressure
Centralization circulatory
Breathing disorders
It was during the agony of the body loses 60 ... 80 grams of weight (due to complete combustion and depletion of cellular ATP reserves), which in some scientific-sounding articles called weighing souls who left after the agony of the body.
![Page 33: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/33.jpg)
Clinical death
Clinical death is characterized by respiratory and cardiac arrest during 5-6 minutes while live brain cells. Clinical death is reversible process of dying. Reversibility depends on the degree of hypoxic changes in the brain.
![Page 34: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/34.jpg)
Biological death
Biological death is the permanent (irreversible) cessation of all biological functions that sustain a living organism.
![Page 35: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/35.jpg)
Signs of biological death
Relative Relative signs of death: Byeloglazov’s sign
(cat eye)While squeezing the eyeball from a deceased pupil becomes oval.
– passive body’s position
– pale colour of the skin
– loss of reflexes and sensority
– loss of pulse and breathing
– cooling of extremities
– Byeloglazov’s sign
![Page 36: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/36.jpg)
Reliable signs of death
early:
postmortem lividity (PML)
changes in the muscles (Cadaveric Rigidity)
cooling of the body
desiccation of the skin
late:
preserving of the body-
-saponification оr
(Adipocere formation),
-mummification
Which destroy the body - -putrefaction
![Page 37: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/37.jpg)
Postmortem Lividity (PML)
After termination of cardiac activity and loss of tone of the vascular wall is a passive movement of blood through the vessels by gravity and concentration it in below the body parts.
![Page 38: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/38.jpg)
Mummification
Mummification – means a modification of process of putrefaction in which there is a dehydration or dessication of all body after death.
![Page 39: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/39.jpg)
Questions to test
![Page 40: Cell damage: necrosis, apoptosis. General D eath](https://reader036.vdocuments.us/reader036/viewer/2022081503/568151eb550346895dc024c2/html5/thumbnails/40.jpg)
And yet life is beautiful
Thank you for your attention