Breeding bean for resistance to
common and halo bacterial blights
Phil Miklas and Deidre Fourie
CBB reaction is rated 1 to 9 in GH and Field tests
1 2 3 5-6 8 9
Xanthomonas axonopodis pv. phaseoli and Xanthomonas axonopodis var fuscans.
Breeding for resistance to CBB is complex
Seed borne
- Quantitative inheritance - Sources of resistance are unadapted - combining resistance sources is necessary for moderate control
- Different resistance genes in leaf, pod, seed
Singh, S. P., and P. N. Miklas. 2015. Breeding common bean for resistance to common blight: a review. Crop Sci. 55:971-984.
B9
Gluc G04.900 U10.900
Y04.1600
P06.600
Y08.1350 AA03.1100 AD08.450
W02.750 Bng228 Bng102 G18.400 P07.350 P07.550
AI07.600 D1096-2 W13.550
AM13.1500 O19.750
D1831 D1338-2
105
CBBBJ
B10
D1476 I07.700
Bng200 AJ14.250 AG18.550 K19.450b
U01.700 X11.700
Bng68 D1580
L04.500 AN11.300
AN03.350
ROF1.1180
Bng218
AM14.1000
89
B11
Bng112
D1228
AL15.1300 Q11.1000 AE07.800 U10.1000
AD12.450 D1308
AD17.950
AJ16.250 G03.850
D1291 Bng145
P2027 W02.950 K10.700 DH20sT
D1512 G08.1200 100
Co-2
Bng104
S18.1500
AD09.950
Bng94 P2062
ROF7.540
S18.650 AD09.700
D1086
ROD3.560 D03.600
W13.350 T08.500
AD12.350 O10.650
AM06.1000
B6
113
U10.2000 U19.500
D0096
G05.1150 U19.350
Cdc-2
V
bc-3
B5
G19.1800
D1080
Diap-1 ROD20b F07.1550 U12.950
K10.1300 H13.300
D1301 AL08.900
AH17.500 Bng162
72
Aco-2
D1198
S18.500 D1157
D1251
CBBBJ
B3
D1066-2
D1132
AN08.800 Q09.1200 R20.400
AD04.1000 AP07.1300
V20.700 AA03.650
D1020 O10.350 Bng165 F10.900 D08.800
D1009 U20.700
AM18.1050 AM10.525 AH05.650
O20.550 Q18.600 W06.700 S08.500 G17.800 I18.1700 Y11.350
B1
Bng171a
W07.1450 Bng122
O13.700 V20.400
Q01.650 D1662 D1327
AD17.350 V12.550
R20.1250 T01.300
D1032
ROD3.930
AN16.700
V12.1050 107
Ppd
SCN, Co-1, Co-x, Co-w
fin
rk, Ane, Ms-8
Co-9, R3
B2
F06.350
ROG19.1490 G19.1500
ROS3.380
[I-B]
T07.550
AG08.700
O12.900 D1287
P07.700
D0166 V12.700 G05.850
G06.1100 U12.500 Bng174
O12.1600 W20.1400
I06.550 AM02.1500
O19.800 AM10.800
O15.1800 ChS
W02.1100 J01.2000
D1595
Bng119b
V10.900 AM07.300
F13.300 AN08.900
175
CBBBJ
B4
Ur-4
Me O20.1000
Bng224 X07.250
Z04.1200 D1325
Rbcs P09.800
G08.1100 X01.950
F08.1600 Bng71 D1298
AM04.950 D1174
Y17.1100 95
Co-9, Co-y, Co-z, Ur-5, Ur-Dorado, Ur-Ouro Negro
CBBXC
109
P
Asp CBBBJ CBBDX CBBBH
B7
Bng191
ChI Bng199 J09.950
Y04.1050 Bng060
Phs
W20.550 P1090
AM13.450 Z04.600 Bng204 H14.550
D0190 Y04.1300
W16.550 AN03.650
I07.1200 AN08.650
D1107
H12.1050b
D1861
Co-10
CBBXC
CBBXC
Ur-9
Ur-12
Ur-BAC6
Ur-11, Ur-3, Ur-Dorado
SAP6, Xap-1 CBBBH
BC420 SCAR CBBPX
CBBPX D1377 X11.1300
Bng12 Pu-a
132 G03.1150
AO11.1000 D1151 I16.900
A10.1100 T08.650
AO11.1200
DJ1kscar
AH05.1000 U20.1150
D1468 D1505
Bng205 O16.600 I08.1500
AJ13.1350 E19.1500 I03.1550 H11.400 L04.950 X15.450 W01.650
E19.1200
AM07.600 Bng73 D1055 133
CBBXC [C-R]
bc-12
Co-4
CBBPX SU91 SCAR
CBBPX
CBBBA
CBBBA
CBBBA CBBPX
CBBBH
CBBBH
CBBBH
CBBS95
CBBS95
CBBS95
CBBBA
B8
Miklas et al. 2006. Euphytica
CBBOV1, OV3,
B9
Gluc G04.900 U10.900
Y04.1600
P06.600
Y08.1350 AA03.1100 AD08.450
W02.750 Bng228 Bng102 G18.400 P07.350 P07.550
AI07.600 D1096-2 W13.550
AM13.1500 O19.750
D1831 D1338-2
105
B10
D1476 I07.700
Bng200 AJ14.250 AG18.550 K19.450b
U01.700 X11.700
Bng68 D1580
L04.500 AN11.300
AN03.350
ROF1.1180
Bng218
AM14.1000
89
B11
Bng112
D1228
AL15.1300 Q11.1000 AE07.800 U10.1000
AD12.450 D1308
AD17.950
AJ16.250 G03.850
D1291 Bng145
P2027 W02.950 K10.700 DH20sT
D1512 G08.1200 100
Co-2
Bng104
S18.1500
AD09.950
Bng94 P2062
ROF7.540
S18.650 AD09.700
D1086
ROD3.560 D03.600
W13.350 T08.500
AD12.350 O10.650
AM06.1000
B6
113
U10.2000 U19.500
D0096
G05.1150 U19.350
Cdc-2
V
bc-3
B5
G19.1800
D1080
Diap-1 ROD20b F07.1550 U12.950
K10.1300 H13.300
D1301 AL08.900
AH17.500 Bng162
72
Aco-2
D1198
S18.500 D1157
D1251
B3
D1066-2
D1132
AN08.800 Q09.1200 R20.400
AD04.1000 AP07.1300
V20.700 AA03.650
D1020 O10.350 Bng165 F10.900 D08.800
D1009 U20.700
AM18.1050 AM10.525 AH05.650
O20.550 Q18.600 W06.700 S08.500 G17.800 I18.1700 Y11.350
B1
Bng171a
W07.1450 Bng122
O13.700 V20.400
Q01.650 D1662 D1327
AD17.350 V12.550
R20.1250 T01.300
D1032
ROD3.930
AN16.700
V12.1050 107
Ppd
SCN, Co-1, Co-x, Co-w
fin
rk, Ane, Ms-8
Co-9, R3
B2
F06.350
ROG19.1490 G19.1500
ROS3.380
[I-B]
T07.550
AG08.700
O12.900 D1287
P07.700
D0166 V12.700 G05.850
G06.1100 U12.500 Bng174
O12.1600 W20.1400
I06.550 AM02.1500
O19.800 AM10.800
O15.1800 ChS
W02.1100 J01.2000
D1595
Bng119b
V10.900 AM07.300
F13.300 AN08.900
175
B4
Ur-4
Me O20.1000
Bng224 X07.250
Z04.1200 D1325
Rbcs P09.800
G08.1100 X01.950
F08.1600 Bng71 D1298
AM04.950 D1174
Y17.1100 95
Co-9, Co-y, Co-z, Ur-5, Ur-Dorado, Ur-Ouro Negro
109
P
Asp
B7
Bng191
ChI Bng199 J09.950
Y04.1050 Bng060
Phs
W20.550 P1090
AM13.450 Z04.600 Bng204 H14.550
D0190 Y04.1300
W16.550 AN03.650
I07.1200 AN08.650
D1107
H12.1050b
D1861
Co-10
Ur-9
Ur-12
Ur-BAC6
Ur-11, Ur-3, Ur-Dorado
SAP6 SCAR CBBXD
BC420 SCAR CBBPX
D1377 X11.1300
Bng12 Pu-a
132 G03.1150
AO11.1000 D1151 I16.900
A10.1100 T08.650
AO11.1200
DJ1kscar
AH05.1000 U20.1150
D1468 D1505
Bng205 O16.600 I08.1500
AJ13.1350 E19.1500 I03.1550 H11.400 L04.950 X15.450 W01.650
E19.1200
AM07.600 Bng73 D1055 133
[C-R]
bc-12
Co-4
CBBPX SU91 SCAR
B8
CBBBJ CBBDX CBBBH
CBBOV1, OV3,
Xa11.1 QTL in VAX-1 & VAX-3
† Trifoliolate leaf ¶ Primary leaf ‡ Pods
0 18 35 53 72
‡
¶
†
cM
Xa11.4
LOD0
† Trifoliolate leaf ¶ Primary leaf ‡ Pods
0 25 50 75 100
cM
‡
¶
†
Xa11.4
LOD0
† Trifoliolate leaf ¶ Primary leaf ‡ Pods
0 25 50 75 100
†
¶
‡
cM
Xa11.4
LOD0
0 15 35 47 55 72
¶ Primary leaf † Trifoliolate leaf ‡ Pods
cM
LOD0
Xa11.4 †
¶
‡
Othello/VAX-1 Aggressive strain
Viteri, D.M., P.B. Cregan, J. Trapp, P.N. Miklas, and S.P. Singh. 2014. A new common bacterial blight resistance QTL in VAX … Crop Sci. 54:1598–1608.
OV3 aggressive
OV1 weak
OV3 weak
QTL (gene) x pathogen strain interaction • Zapata. 1997. Identification of Xanthomonas campestris pv. phaseoli races
in P. vulgaris leaves. Agron. Mesoam. 8:44–52.
• Mutlu, Steadman, et al. 2008. Differential pathogenicity of Xanthomonas campestris pv. phaseoli and X. fuscans subsp. fuscans) strains on bean genotypes with CBB. Plant Dis. 92:546–554.
• Viteri, Miklas, Singh et al. 2014. ……interaction of the new QTL, SAP6, and SU91 with bacterial strains. Crop Sci. 54:1598–1608.
Strain Genotype QTL Weak Aggressive Othello None 7.2 8.8 VAX 3 SAP6, SU91, Xa11.4 2.8 3.2 15 RILs SAP6 3.0 d 7.4 a 10 RILs SU91 5.4 b 6.7 b 16 RILs Xa11.4 4.9 c 6.0 c
Recessive epistasis BC420 and SU91 QTL Teebus*6 /XAN159 F2
8.3 8.1 8
3.3 3.21.7 1.6 1.2 1.1
13579
bbss
Bbss
BBss
bbSS
bbSs
BbSS
BbSs
BBSs
BBSS
Genotype
Dise
ase
scor
e
9 3
4
Vandemark, G.J., D. Fourie, and P.N. Miklas. 2008. TAG 117:513–522.
Effect of combined sources on level of resistance to CBB in dry bean
Dry bean lines
P. vulgaris SAP 6
P. vulgaris PI 207262 Pv07
Scarlet runner bean
Tepary bean SU91, BC420, Xa11.4
Disease score
Chase, Montcalm X 6,7
XAN 112, BAT93 X X 5,6
XR-235, etc X 5,6
USPT-CBB-1 X X 4,5
XAN -159 X X 2,3
USDK-CBB-15 X X 4,5
VAX-3, VAX-4 X X X X 1,2
Miklas et al 2006. Common bean breeding for resistance against biotic and abiotic stresses: from classical to MAS breeding. Euphytica 147:105-131.
1
3
5
7
9
USDK-
15
USDK-
14
Montca
lm
XAN 15
9
Dis
ease
sco
re
CBB reaction of kidney lines bred for resistance using MAS
SAP6 SU91
SAP6 SU91
BC420 SU91
SAP6
Miklas et al. 2006. Registration of USDK-CBB-15 dark red kidney bean germplasm line with improved resistance to common bacterial blight. Crop Sci. 46:1005-1006.
Viteri et al.. 2014. Progress in breeding Andean common bean for resistance to common bacterial blight. Crop Sci. 54:2084–2092.
SAP6 QTL marker on Pv10
• Miklas et al. 1996. Selective mapping of QTL conditioning disease resistance in common bean. Crop Sci. 36:1344-1351.
RAPD AP06
• Miklas et al. 2000. MAS for pyramided resistance to CBB in common bean. Ann. Rept. Bean Improv. Coop. 43:39-40.
AP06 converted to SCAR SAP6
• Miklas et al. 2003. A major QTL for CBB resistance derives great northern landrace cultivar Montana No.5. Euphytica 131:137-146.
SAP6 derives from common bean not tepary bean
cM
‡
†
¶
SAP6 QTL physical position
Viteri et al. 2014. A new CBB resistance QTL in VAX 1 and interaction of the new QTL, SAP6, and SU91 with Xcp strains. Crop Sci. 54:1598-1608.
Marker position
SAP6 39,939,000
KASP (B. Raatz) 40,020,540
SNP 47812 40,517,575
Caution – SAP6 marker is found in susceptible germplasm so is not diagnostic and can be tricky to use
BC420 SCAR marker on Pv06 • Jung et al. 1997. CBB resistance mapped in PC-50/XAN-159 J. Am. Soc.
Hortic. Sci. 122:329–337. LG5 QTL • Park et al. 1999. Confirmation of molecular markers and flower color
associated with QTL for resistance to CBB. JASHS 124:519–526. LG5 QTL and V locus for flower/seed color tightly linked • Yu, K., S.J. Park, and V. Poysa. 2000. MAS of beans for resistance to CBB:
Efficacy and economics. Plant Breed. 119:411–415. Describe BC420 SCAR marker • Vandemark, G.J., D. Fourie, and P.N. Miklas. 2008. …..recessive epistasis
between QTL conferring resistance to CBB. TAG 117:513–522. BC420 QTL is not effective on its own • Shi et al. 2012. Development of candidate gene markers associated to
CBB resistance in common bean. TAG 125:1525-1537. BC420 CG14 – co-dominant marker
SU91 SCAR marker on Pv08
• Pedraza et al. 1997. SU91 marker for CBB resistance QTL. CIAT publ.
SU91 QTL (dominant SCAR)
• Shi et al. 2012. Development of candidate gene markers associated to CBB resistance in common bean. TAG 125:1525-1537.
SU91 CG-11 – co-dominant marker
• Bett et al. KASP assay (SU91 QTL) - unpublished
SU91 KASP marker
• Raatz et al. KASP assay (SU91 QTL) - unpublished SU91 KASP marker (100 kb distal)
Future considerations
• A combination of phenotypic and MAS is a useful approach for improving resistance to CBB
• PI 207262 resistance should be analyzed separately for unique
QTL on Pv07 and marker developed.
• VAX lines may have Pv07 QTL? Use RILs absent SAP6, SU91, and Xa11.4 leaving just Pv07 QTL segregating.
• Need to further validate Xa11.4 QTL and develop marker (in progress)
• Coordination of efforts – sharing of resistant breeding lines?
Line Seed type BCMV resistance CBB resistance AO-1012-29-3-3A Dark red kidney I + bc-1(2) No XRAV-40-4 Black I + bc-3 No DPC-40 Black I + bc-3 No Raven Black I + bc-3 No Verano White I gene Yes PR0806-80, -81 White I + bc-3 No PR0401-259, PR0650-31
Pink Black
I gene I gene
Yes Yes
PR0633-10, PR0737-1
Red mottled I + bc-3 I + bc-3
Yes No
Badillo Light red kidney I gene Yes PR9443-4 Light red kidney I gene Yes VR-1s, -7s, -8s White I + bc-3 No PR9357-107 Small red I + bc-3 No TARS-LFR1 Small red I gene Yes 08SH-840 cranberry I gene Yes USCR-7, USCR-9 Cranberry I + bc-3 No USLK-2 USDK-4 USWK-6
Light red - Dark red -
White - kidneys
I + bc-3 No
USDK-CBB-15 Dark red kidney I Yes USWK-CBB-17 White kidney I Yes USCR-CBB-20 Cranberry I Yes Werna, RS-7 Sugar I Yes
Halo bacterial blight Pseudomonas syringae pv. phaseolicola Pseudomonas savastanoi pv. phaseolicola
HB reaction is rated 1 to 5 in Greenhouse
1 5
HBB: host x pathogen interaction Teverson, D.M. 1991. Genetics of pathogenicity and resistance in the halo-blight disease of beans in Africa. Ph.D. Dissertation. Univ. of Birmingham, UK. p. 205. Taylor, Teverson, Allen, and Pastor-Corrales. 1996. Identification and origin of races of Psp from Africa and other bean growing areas. Plant Pathol. 45:469–478. Taylor, Teverson, and Davis. 1996. Sources of resistance to Psp races in P. vulgaris. Plant Pathol. 45:479–85
Differential
R-genes
Race 1 2 3 4 5 6 7 8 9
Canadian Wonder - + + + + + + + + + ZAA 54 (A52) 4 + + + + - + + + + Tendergreen 3 + + - - + + + + + UI 3 1,4 - + + + - + - + - P.acutifolius (1072) 2 + - + - - + - + + ZAA 55 (A53) 3,4 + + - - - + + + + ZAA 12 (A43) 2,3,4,5 + - - - - + - - - Guatemala 196-B 1,3,4 - + - - - + - + -
HBB: host x pathogen interaction Differential
R-genes
Race
1 2 3 4 5 6 7 8 9
Canadian Wonder - + + + + + + + + +
ZAA 54 (A52) 4 + + + + - + + + +
Tendergreen 3 + + -HR -HR + + + + +
UI 3 1,4 - + + + - + - + -
P.acutifolius (1072) 2 + - + - - + - + +
ZAA 55 (A53) 3,4 + + -HR -HR - + + + +
ZAA 12 (A43) 2,3,4,5 + - -HR -HR - + - - -
Guatemala 196-B 1,3,4 - + -HR -HR - + - + -
Rating of 1 = - incompatible, and 2-5 = + compatible (susceptible)
HBB: host x pathogen interaction
Differential
R-genes
Race 1 2 3 4 5 6 7 8 9
Canadian Wonder - + + + + + + + + +
ZAA 54 (A52) Pse-4 + + + + - + + + +
Tendergreen Pse-3 + + -HR -HR + + + + +
Rating of 1 = - incompatible, and 2-5 = + compatible (susceptible)
M10.1000 0.0
F9.1000 2.7 X13.420 SR13.1150 ST8.1350 Pse-1 SH11.800
3.6
BA
AM18.1300
P11.550 0.0
V10.300 4.7
U20.780 6.6 I10.1350 6.8
9.5 M10.1000 12.3 AG15.660 12.9 X13.420 13.7
SH11.800 15.3 P7.750 16.0
P1.650 16.7 H11.640 17.0
X3.480 19.3 Q9.520 23.6
U10.510 26.2
CU
WX11.2 0.0 WV17.2 0.6 Bng200 2.2
DROE9c 4.0
WG16.1 8.0 ST8.1350 11.1 WP08.3 11.6 X13.420 11.7 SR13.1150 F9.1000 11.8 DROE9a 12.7
WAG15.1 17.9
WR16.1 22.3
Bng68 30.4
BJ Pv10 HB- QTL
FW - QTL
ALS - QTL
Halo blight R genes: Pse-1 on Pv10
Miklas, Fourie, et al. 2009. Tagged and mapped Pse-1 gene for resistance to HBB in host cultivar UI-3. Crop Sci. 49:41–48.
Differential
R-genes
Race 1 2 3 4 5 6 7 8 9
UI 3 Pse-1, Pse-4 - + + + - + - + -
Miklas, Fourie, Trapp, Larsen, Chavarro, Blair, and Gepts. 2011. Characterization and mapping of Pse-2 gene for resistance to HBB. Crop Sci. 51:2439–2448.
Halo blight R genes: Pse-2 on Pv10
Differential
R-genes
Race
1 2 3 4 5 6 7 8 9
ZAA 12 (A43) 2,3,4,5 + - -HR -HR - + - - -
Pse-2 + - -HR -HR - + - - -
Pse-3 + + -HR -HR + + + + +
Pse-4 + + + + - + + + +
HBB: host x pathogen interaction: Pse-6 on Pv04
Differential
R-genes
Race 1 2 3 4 5 6 7 8 9
UI 3 Pse-1, Pse-4 - + + + - + - + -
BelNeb-RR-1 Pse-4, Pse-6 - + + + - + - + -
Rating of 1 = - incompatible, and 2-5 = + compatible (susceptible)
Miklas, Fourie, et al. 2014. New loci including Pse-6 conferring resistance to halo bacterial blight on chromosome Pv04 in common bean. Crop Sci. 54: 2099-2108.
HBB: host x pathogen interaction: Pse-6 on Pv04
Differential
R-genes
Race
1 2 3 4 5 6 7 8 9
UI 3 Pse-1, Pse-4 - + + + - + - + -
BelNeb-RR-1 Pse-4, Pse-6 - + + + - + - + -
I9365-31 Race 1 near Pse-6
- + -HR -HR - + + + +
Minuette Race 7 nearPse-6
+ + -HR -HR - + - + +
Rating of 1 = - incompatible, and 2-5 = + compatible (susceptible)
Miklas, Fourie, et al. 2014. New loci including Pse-6 conferring resistance to halo bacterial blight on chromosome Pv04 in common bean. Crop Sci. 54: 2099-2108.
Race7 0.0 BM161 4.3 PVctt001 7.0 SB10 7.2 ss646898 10.1 ss642306 10.7 ss649778 11.1 ss648677 11.8 ss646903 13.2 ss646908 13.5 ss649971 13.8 ss647820 14.2 ss647823 14.9 ss647807 15.5 ss647813 16.6 ss647811 18.7 ss647817 20.8 ss650010 21.5 ss650014 21.9 ss646246 22.6 ss646249 22.9 ss646240 23.6 ss646223 25.3 ss646218 26.4 ss5646216 27.1
ss640555 ss648115 43.9 ss648114 44.2 ss647600 45.6 ss647590 45.9 ss648321 46.6 ss648228 47.3 ss645162 47.7 ss648226 ss648327 48.3 ss641934 ss641932 49.4 ss640046 49.7
Pv04 BM1610.0
PV-ctt0013.3
Race14.4
F18/Em6-2907.4
F18/R11-29010.1
Mapping Pse-6, Race 1, Race 7 BA RIL MO RIL
R31 RIL
HBB: host x pathogen interaction Differential
R-genes
Race 1 2 3 4 5 6 7 8 9
Canadian Wonder - + + + + + + + + +
ZAA 54 (A52) 4 + + + + - + + + +
Tendergreen 3 + + -HR -HR + + + + +
UI 3 1,4 - + + + - + - + -
P.acutifolius (1072) 2 + - + - - + - + +
ZAA 55 (A53) 3,4 + + -HR -HR - + + + +
ZAA 12 (A43) 2,3,4,5 + - -HR -HR - + - - -
Guatemala 196-B 1,3,4 - + -HR -HR - + - + -
Rating of 1 = - incompatible, and 2-5 = + compatible (susceptible)
Quantitative resistance to HBB
Taylor et al. 1996. Screened 1048 dry bean lines and • 50% had at least one R gene • most common was Pse-3 and Pse-4 • least common was Pse-2 • 1% exhibited quantitative resistance
Duncan et al. 2014. Inheritance of resistance to Race 6 of Psp in Pinto Bean US14HBR6 • Two independent recessive genes
414 ADP lines screened for reaction to Race 6 in the field in three reps, Potch-2014
Fourie, Miklas, Porch, and Talo
1.0
3.0
5.0
7.0
9.0
0 100 200 300 400 500
Dise
ase
scor
e
ADP lines
Screening ADP nursery for Race 6 resistance
Fig 1 and 2. Manhattan and QQ Plot of Genome Wide Association Analysis of Halo Blight Score in Potch, South Africa, 2014
362 ANDEAN ADP LINES; 11,995 SNPS (0.05 MAF); MLM, KINSHIP AND 3 PRINCIPAL COMPONENTS; Halo Blight DATA: TRANSFOMED TO NATURAL LOG 2; significance via Bonferroni correction (4.17E-06 )
HBB trial Potch 2014, Yield, MLM, 6 PC, 365 genotype, 2663 SNPs 362 Andean ADP lines
Quantitative resistance to HBB (Race 6)
Differential
R-genes
Race
1 2 3 4 5 6 7 8 9
CAL 143 ? 1.9 1.3 1.3 1.9 1.1 1.8 1.7 1.6 1.7
Rojo ? + + -HR -HR + + - - +
Table 1. Reaction of the parents Rojo/CAL 143 RIL mapping population (144 RILs) to four races of the pathogen in the GH.
Ratings 2.5 or less but greater than 1 are considered quantitative resistant reactions.
Figure 2. SNP linkage map of chromosome Pv04 depicting QTL conditioning resistance to races 1, 6, 8. Field reaction in Rojo/CAL 143 to Race 6 confirms same QTL.
SNP640712 0.0 SNP639783 5.3 SNP648135 9.1 SNP645076 12.6 SNP639890 13.8 SNP639886 14.3 SNP639704 14.6 SNP640509 15.7 SNP648635 15.9 SNP640686 16.5 SNP639759 17.6 SNP641800 19.4 Race7 36.8 Race8 41.1 SNP644248 47.3 SNP650460 48.4 SNP640418 49.5 SNP649366 53.3 SNP639432 61.3 SNP649371 62.5 SNP647202 64.4 SNP647215 66.0 SNP648592 67.0 SNP648595 68.4 SNP646316 69.8 SNP646332 71.8 SNP646336 72.5 SNP646341 73.5 SNP646346 75.6 SNP650584 76.6 SNP647912 77.0 SNP639212 77.6 SNP645507 78.0 SNP645514 79.8 SNP645524 81.2 SNP645529 82.6 SNP645533 83.4
Pv04
Pv10
HBB resistance genes and QTL
Gene Races Chromosome Mb Markers MAS Pse-1 1, 5, 7, 9 Pv10 11.2 or 41.0 SCARs No Pse-2 2, 3, 4, 5, 7, 8, 9 Pv10 3.5 SCAR Yes Pse-4 5 Pv10 39.6 SNPs No Rojo 7, 8 Pv10 9.8 to 17.7 SNPs No Pse-6 1, 5, 7, 9 Pv04 0.6 SCAR Yes I9365-1 1 Pv04 0.5 SSR No Minuette 7 Pv04 0.2 SNPs No CAL 143 QTL - 1, 6, 8 Pv04 0.5 SNPs No
ADP-AM QTL - 6 Pv05 38.7 SNPs No Pse-3 3, 4 Pv02 48.2 I gene markers Yes
ADP lines resistance to Race 6 – in field for 2 years
ADP line Name Disease
score
ADP-121 Kranskop HR-1 1.0 ADP-126 SELIAN 05 1.0 ADP-125 CHEUPE 1.0 ADP-114 OPS-RS1 1.0 ADP-120 Tygerberg 1.0 ADP-432 PR0637-134 1.0 ADP-0798 KAMIESBERG 1.0 ADP-379 PI 203934 1.0 ADP-118 Werna 1.2 ADP-0797 RS 7 1.2 ADP-123 Jenny 1.2 ADP-728 GCI -LRK-291-RAR 1.2 ADP-716 SUGAR 131 1.3 ADP-454 INIAP 429 1.3 ADP-522 Amarelo, Cela 1.3 ADP-517 Carioca, Kibala 1.3 ADP-122 Kranskop 1.3
ADP line Name Disease
score ADP-730 GCI-CAL-272-AR-2 1.3 ADP-84 KABLANKETI NDEFU 1.5 ADP-276 G 13654 1.5 ADP-592 AND 1005 1.5 ADP-508 Calembe 1.7 ADP-255 G 10994 1.8 ADP-737 KG 27-8 1.8 ADP-80 KABLANKETI 1.8 ADP-740 CIM-RM05-ALS-82 2.0 ADP-526) CAL-143 2.1 ADP-723 KAB 06 F2.8-69 2.2 ADP-461 PI527540-A 2.2 ADP-537 AFR 619 2.3 ADP-572 NUA56 2.5 ADP-736 KG 15-26 2.5 ADP-739 UYOLE 03 2.5 ADP-566 G5686 2.8