![Page 1: BOD Troubleshooting [Read-Only] - BOD Troubleshooting.pdf6/8/2012 3 Seeding Purchased/Use Own Depletion of 0.6-1.0 mg/L per mL of seed Run a GGA on seed Seed control bottle Don’t](https://reader034.vdocuments.us/reader034/viewer/2022042211/5eb2c0dc3a37ec75f479a87d/html5/thumbnails/1.jpg)
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Amy Vail
City of Cadillac
Lab Manager
Overview� Sampling
� Pretreatment
� Seeding
� Procedure
� Blanks
� Glucose-Glutamic Acid
� Duplicates
![Page 2: BOD Troubleshooting [Read-Only] - BOD Troubleshooting.pdf6/8/2012 3 Seeding Purchased/Use Own Depletion of 0.6-1.0 mg/L per mL of seed Run a GGA on seed Seed control bottle Don’t](https://reader034.vdocuments.us/reader034/viewer/2022042211/5eb2c0dc3a37ec75f479a87d/html5/thumbnails/2.jpg)
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Sampling� Location
� Size
� Preservation
� Hold Time
Pretreatment� Representative Sample
� Mixing
� Dilutions
� Temperature
� pH Adjustment/Seeding
� Dechlorination/Seeding
� Nitrification Inhibitor
![Page 3: BOD Troubleshooting [Read-Only] - BOD Troubleshooting.pdf6/8/2012 3 Seeding Purchased/Use Own Depletion of 0.6-1.0 mg/L per mL of seed Run a GGA on seed Seed control bottle Don’t](https://reader034.vdocuments.us/reader034/viewer/2022042211/5eb2c0dc3a37ec75f479a87d/html5/thumbnails/3.jpg)
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Seeding� Purchased/Use Own
� Depletion of 0.6-1.0 mg/L per mL of seed
� Run a GGA on seed
� Seed control bottle
� Don’t add to dry bottle
Procedure� Consistency
� Cleanliness
� Bottles
� Valid Results
� 1.0 mg/L Residual
� 2.0 mg/L Depletion
![Page 4: BOD Troubleshooting [Read-Only] - BOD Troubleshooting.pdf6/8/2012 3 Seeding Purchased/Use Own Depletion of 0.6-1.0 mg/L per mL of seed Run a GGA on seed Seed control bottle Don’t](https://reader034.vdocuments.us/reader034/viewer/2022042211/5eb2c0dc3a37ec75f479a87d/html5/thumbnails/4.jpg)
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Procedure� Dilution Water
� Make Up (CBOD v. BOD)
� Aeration
� Storage
� Nutrient Mixture
Procedure� Dissolved Oxygen Measurement
� Calibration
� Winkler
� Electrode
� Measurements
� Stirring
� Rinsing
� Blank Recheck
![Page 5: BOD Troubleshooting [Read-Only] - BOD Troubleshooting.pdf6/8/2012 3 Seeding Purchased/Use Own Depletion of 0.6-1.0 mg/L per mL of seed Run a GGA on seed Seed control bottle Don’t](https://reader034.vdocuments.us/reader034/viewer/2022042211/5eb2c0dc3a37ec75f479a87d/html5/thumbnails/5.jpg)
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Procedure� Preparation for Incubation
� Water Seal
� Capping
� Incubation
� 20 +/- 1°C for 5 days +/- 6 hours
Blanks� <0.2 mg/L Depletion
� Glassware
� Supersaturation
� Temperature
� Atmospheric Pressure
� Contaminated Source Water
� Nitrification Inhibitor
� Procedure/Operator Difference
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Glucose-Glutamic Acid� 198 +/- 30.5 mg/L BOD/172 +/- 30.5 mg/L CBOD
� Seed too strong/weak
� Making solution improperly
� Procedure
Duplicates� Consistent Source
� Consistent Procedure