![Page 1: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/1.jpg)
Bioengineering:Engineering Applications For The Real World
CU Science DiscoverySchool and Teacher Programs
![Page 2: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/2.jpg)
Bioengineering is:the biological and medical applications of engineering
principles, practices, tools and technologies
![Page 3: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/3.jpg)
Bioengineering at CU
Bioastronautics Chemical and Biological
Biomedical EngineeringEnvironmental Engineering
![Page 4: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/4.jpg)
Three Activities:Design a Custom Bacteria
Protect That Pill
Alginate Encapsulation
BioengineeringPractice using
engineering design process models in
bioscience applications from the real world
75 Minutes of Activities
![Page 5: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/5.jpg)
Design Process ModelsEngineering is Elementary Design Process
Model
![Page 6: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/6.jpg)
Bacteria Taxonomy
![Page 7: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/7.jpg)
Bacteria Name: Escherichia coliPronunciation: esh-er-i-kia coal-iAbbreviation: E. Coli
Bacteria Raw Materials
Environments: Warm fresh water with temperature limited to 10 to 40 C (50-104 F)
Applications: Sewage, Biological Waste and Simple Organic Compounds
E. coliMobile with Flagella or tails
E. coliCapsule has pila that allow it to form bio-films
E. coliGenetic option to create non-reproducing strains
E. coliEnergy from organic compounds
E. coliReproduction is rapid with option for sterile strains
E. coliHas Pathology, some can cause diseases in people
E. coliMembrane is sensitive to salts, metals & chemicals
![Page 8: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/8.jpg)
Bacteria Name: Dechloromonas aromatica Pronunciation: De-cloro-moan-us aro-mat-icaAbbreviation: D. aromatic
Bacteria Raw Materials
D. aromaticaLimited Mobility with Flagella/Tails
D. aromaticaCapsule cannot tolerate any oxygen
D. aromaticaNo special Genetic features
D. aromaticaEnergy from benzene and perchlorates
D. aromaticaReproduction is very slow and limited
D. aromaticaHas no Pathology, not disease causing
D. aromaticaCell Membrane is salt tolerant
Environments: Soils, deep water and oxygen free environments with average temperatures of 1-32 C (33-90 F)
Applications: Perchlorates (salts used in industry/rocket fuel) and Benzene (a persistent industrial pollutant)
![Page 9: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/9.jpg)
Bacteria Name: Pseudomonas putidaPronunciation: Su-doe-mon-as pu-tee-daAbbreviation: P. putida
Bacteria Raw Materials
Environments: Warm, very well oxygenated salt or fresh water from 20 to 35 C (68-95 F)
Applications: Oil, plastics and oil-based pesticides (Atrazine)
P. putidaFull Mobility with Flagella or tails
P. putidaCell Wall is rigid and resistant to high pressures
P. putidaNo special Genetic features
P. putidaBreaks down oil and plastics for Energy
P. putidaReproduction is rapid in limited environments
P. putidaHas Pathology, some cause diseases in people
P. putidaCell Membrane is very salt and oil tolerant-resistant
![Page 10: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/10.jpg)
Bacteria Name: Nitrosamonas europeaPronunciation: Nitro-samon-as euro-peaAbbreviation: N. europea
Bacteria Raw Materials
Environments: Soils, sewage and fresh water but with very limited temperature ranges of 20-30 C (68-86 F)
Applications: Ammonia and ammonia-based compounds (fertilizers)
N. europeaNo Mobility
N. europeaCell Wall is non-rigid and sensitive to high pressure
N. europeaGenetic option to limit reproduction to 5 life cycles
N. europeaBreaks down ammonia for Energy
N. europeaReproduction is very slow and can be limited
N. europeaHas no Pathology, not disease causing
N. europeaCell Membrane is resistant to nitrogen & organics
![Page 11: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/11.jpg)
Bacteria Name: Deinococcus radiodurans Pronunciation: Dee-no-coch-us radio-dur-ansAbbreviation: D. radiodurans
Bacteria Raw Materials
Environments: Soils, fresh water, extreme temperatures (20-55 C), dry environments but not salts or salt water
Applications: Radioactive Waste, Heavy Metals and Mercury
D. radioduransNo Mobility
D. radioduransCapsule resists heat, cold, light, radiation, but not salts
D. radioduransNo special Genetic features
D. radioduransEnergy from organic and some inorganic chemicals
D. radioduransReproduction is rapid and in extreme environments
D. radioduransHas no Pathology, not disease causing
D. radioduransCell Membrane resists dehydration & chemicals
![Page 12: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/12.jpg)
Narrative: We have a crisis that could make or break a small business in rural Colorado. A small-scale processor of organic foods has found pesticides, specifically Atrazine, in a shipment of soybeans they have already started to process! Their grinding equipment needs to be cleaned up fast so they can get back to work but they cannot just wash the atrazine down the drain. The grinders they used are in a very cold warehouse and have a lot of ‘nooks and crannies” so they need a mobile organism, but not one that can ‘get away’. Most importantly, we have to guarantee that the organism will not stick around after the treatment, cause diseases, or get into the food system!
Design Challenge #1
![Page 13: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/13.jpg)
Design Challenge #1Design Considerations and Environment:
Grinding equipment is contaminated with Atrazine (an oil-based pesticide)
They need limited mobility to get into the small spaces, but not one that can get away!
The warehouse is very cold and has bright lights (high UV light)
The soybeans left behind organic chemicals and Nitrogen compounds
The organisms need to be able to get their energy from organic compounds
Organisms must NOT be able to survive, even for a day, after treatment
The organisms cannot cause diseases in people
![Page 14: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/14.jpg)
3-E EngineeringEffective: Will the proposed solution work well and does it meet ALL of the proposed design specifications?Economic: Is the proposed solution done in a way that maximizes product efficiency and return on investment?Ethical: Can the proposed solution cause any harm to people or systems in the long and short term?
![Page 15: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/15.jpg)
Design Process ModelsEngineering is Elementary Design Process
Model
![Page 16: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/16.jpg)
Design Process ModelsEngineering is Elementary Design Process
Model
Insert Design Process Model Images from:www.engr.ncsu.edu
![Page 17: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/17.jpg)
3-E EngineeringEffective: Will the proposed solution work well and does it meet ALL of the proposed design specifications?Economic: Is the proposed solution done in a way that maximizes product efficiency and return on investment?Ethical: Can the proposed solution cause any harm to people or systems in the long and short term?
![Page 18: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/18.jpg)
![Page 19: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/19.jpg)
Component # of Spoonfuls Fraction
Water 1 1/5
Oil 1 1/5
Flour 3 3/5
Corn Starch 0 0/5
Salt 0 0/5
Sugar 0 0/5
Total 5 5/5ths
![Page 20: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/20.jpg)
Alginate Encapsulation
Polymers are chemical
compounds made from repeating
chains or networks of
smaller compounds called
monomers
![Page 21: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/21.jpg)
Alginate Encapsulation
Insert Images From: http://openwetware.org/wiki/IGEM:IMPERIAL/2009/Encapsulation/Phase2/Alginate_Properties
![Page 22: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/22.jpg)
Bioengineering is:the biological and medical applications of engineering
principles, practices, tools and technologies
![Page 23: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/23.jpg)
Bioengineering at CU
Bioastronautics Chemical and Biological
Biomedical EngineeringEnvironmental Engineering
![Page 24: Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs](https://reader035.vdocuments.us/reader035/viewer/2022081518/551c5233550346a5458b4d94/html5/thumbnails/24.jpg)
Bioengineering:Engineering Applications From The Real World
CU Science DiscoverySchool and Teacher Programs