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Amplification Reagents and Plastics

Amplification: Consumables

http://www.bio-rad.com

Factors Impacting Gene Expression Analysis

RNA Isolation■■ RNA integrity, purity, and yield■■ Genomic DNA contamination■■ Inhibitors of cDNA synthesis and qPCR■■ RNase and DNase contamination

Reagents — Reverse Transcription■■ cDNA synthesis efficiency■■ RNA protection■■ Input RNA capacity ■■ Accurate representation of mRNA

Reagents — Real-Time qPCR■■ Detection sensitivity■■ Assay specificity■■ Inhibitors in sample■■ Reproducibility of thermal cycling conditions and instrument compatibility

PCR Plastic Consumables■■ Instrument compatibility■■ Optimum performance■■ Automation friendly■■ Potential source of contamination and inhibition

RNA Isolation ■■ Kits are designed and formulated to assist in the isolation of highly pure and intact RNA from different starting materials

■■ RNA is compatible with a variety of downstream applications – Real-time qPCR – Northern blotting – Microarray analysis – cDNA library construction

■■ DNase treatment ensures genomic DNA removal

© 2011 Bio-Rad Laboratories, Inc. Amplification Reagents and Plastics 5

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732-6800, 2 x 96-well preps Aurum Total RNA 96 Kit

RNA Isolation ■■ Kits are designed and formulated to assist in the isolation of highly pure and intact RNA from different starting materials

■■ RNA is compatible with a variety of downstream applications – Real-time qPCR – Northern blotting – Microarray analysis – cDNA library construction

■■ DNase treatment ensures genomic DNA removal

Aurum™ Total RNA Fatty and Fibrous Tissue Kit■■ PCR-ready RNA in less than 60 min

■■ PureZOL™ efficiently lyses cells and tissues, deproteinates RNA, and inactivates endogenous nucleases in a single step

■■ High yield of intact total RNA from difficult-to-disrupt samples, including plant and animal tissues

■■ Well suited for fungal samples that are rich in RNases

■■ RNase-free reagents and plastic consumables ensure the integrity of isolated RNA

■■ Kit includes DNase I for removal of genomic DNA contamination

■■ Easy-to-use spin or vacuum protocol

For more information, request bulletin 5282.

Aurum Total RNA Mini Kit ■■ PCR-ready RNA in less than 60 min

■■ Guanidine isothiocyanate and β-mercaptoethanol efficiently lyse samples and quickly inactivate RNases

■■ High yield of intact total RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as plant and animal tissues



■■ Easy-to-use spin or vacuum protocol


Aurum Total RNA 96 Kit ■■ High-throughput total RNA isolation in less than 60 min

■■ High yield of intact total RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as plant and animal tissues

■■ Guanidine isothiocyanate and β-mercaptoethanol efficiently lyse samples and quickly inactivate RNases



■■ Compatible with Aurum vacuum manifold


RNA Isolation

732-6820, 50 preps Aurum Total RNA Mini Kit

732-6830, 50 preps Aurum Total RNA Fatty and Fibrous Tissue Kit

www.bio-rad.com/rna-isolation

http://www.bio-rad.com/rna-isolation

http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_2919.pdf



http://www.bio-rad.com/rna-isolation




Amplification Reagents and Plastics Visit us on the Web at www.bio-rad.com.6

iScript RT-qPCR sample preparation reagent generates linear results over varying input cell amounts. HeLa cells (125, 25, 5, and 1 cells/µl) were treated and analyzed for GAPDH expression levels using iScript cDNA synthesis kit and iQ™ SYBR® Green supermix on the CFX96™ real-time PCR detection system. RFU, relative fluorescence units.

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iScript™ RT-qPCR Sample Preparation Reagent■■ Reagent stabilizes RNA and removes genomic DNA in less than 10 min

■■ Suitable for adherent or suspension animal cells

■■ RT-qPCR is directly enabled from cells without RNA purification when combined with an iScript reverse transcription kit and real-time supermix

■■ Reagent allows multiplex real-time detection of up to 4 targets from as few as 10 cells

■■ Ideal for rapid, high-throughput gene expression analysis


PureZOL™ RNA Isolation Reagent■■ Single-solution format permits recovery of RNA from small quantities of

tissues or cells, making it ideally suited for gene expression studies

■■ Efficient RNA purification from cultured cells, yeast, viruses, and bacteria, as well as plant and animal tissues

■■ PureZOL efficiently lyses cells and tissues, deproteinates RNA, and inactivates endogenous nucleases in a single step

■■ Scalable starting sample amount

■■ Convenient isolation of RNA, DNA, and protein from the same sample

Aurum™ Vacuum Manifold■■ Vacuum-mediated nucleic acid purification platform

■■ Versatile manifold format adaptable for 96-well plate or up to 18 spin columns

■■ Manifold ensures fast, high-quality sample preparation while maintaining the simplicity of vacuum processing

■■ Unique vacuum regulator design allows for complete control of negative pressure

170-8899, 5 x 10 mliScript RT-qPCR Sample Preparation Reagent

732-6890, 100 ml PureZOL RNA Isolation Reagent

RNA Isolation

732-6470, 1 unit Aurum Vacuum Manifold

www.bio-rad.com/iscript

http://www.bio-rad.com/iscript



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Aurum Total RNA Kits PureZOL RNA Mini Fatty and Fibrous Tissue 96 Isolation Reagent

Format Mini column Mini column 96-well plate Single solution Filtration (vacuum or spin) Filtration (vacuum or spin) Filtration (vacuum or spin) organic extraction

Maximum starting material amounts Cultured cells 2 x 106 1 x 107 1 x 106 1 x 107 Bacterial cells 2.4 x 109 2.4 x 109 8 x 108 2.4 x 109 Yeast cells 3 x 107 3 x 107 2 x 107 3 x 107 Hard animal tissue 20 mg 100 mg — 100 mg Soft to moderately 40 mg 100 mg — 100 mg hard animal tissue Plant tissue 40 mg 100 mg — 100 mg

Isolation method Silica membrane Lysis with PureZOL reagent, Silica membrane Organic extraction purification on silica membrane

Number of preps 50 mini preps 50 mini preps 2 x 96-well plate 50 or 100 (1 ml/prep)

Number of washes 3 3 3 —

DNase I included* Yes Yes Yes No

DNase I digest time 15 min (animal tissue, 25 min) 15 min 10 min —

Total preparation time** <50–80 min (with DNase I digest) <50–80 min (with DNase I digest) <60 min (with DNase I digest) <60 min

Binding capacity >100 µg >100 µg >40 µg —

Elution volume 2 x 40 µl 2 x 40 µl 80 µl 30–100 µl

* Removal not required. ** Total preparation time will vary depending on the tissue or cell type and on which format is used (vacuum or spin).

For sample-specific yield information, please visit www.bio-rad.com/rna-isolation and click the RNA Isolation Selection Guide.

RNA Isolation Selection Guide

Ordering Information

Catalog # Description Catalog # Description

732-6830 Aurum Total RNA Fatty and Fibrous Tissue Kit 170-8899 iScript RT-qPCR Sample Preparation Reagent, 500 reactions 732-6870* Aurum Total RNA Fatty and Fibrous Tissue Module 732-6880 PureZOL RNA Isolation Reagent, 50 ml 732-6820 Aurum Total RNA Mini Kit 732-6890 PureZOL RNA Isolation Reagent, 100 ml732-6800 Aurum Total RNA 96 Kit 732-6470 Aurum Vacuum Manifold170-8898 iScript RT-qPCR Sample Preparation Reagent, 100 reactions

* Not provided with PureZOL RNA isolation reagent (see catalog #732-6890 or #732-6880 to order separately).

Reagents — Reverse Transcription ■■ Formulated for efficient reverse transcription across a broad linear dynamic range

■■ Potent RNase A inhibitors protect RNA during setup and reverse transcription

■■ Flexible input RNA capacity to suit different experimental needs

■■ Optimized for gene expression analysis using real-time qPCR


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ReagentsReverse Transcription — iScript™ Kit Selector

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Select my own primers

One-step RT-qPCR with SYBR® Green

One-step RT-qPCR for

probes

iScript one-step RT-PCR kit for probes

iScript™ one-step RT-PCR kit

with SYBR® Green

iScript Select cDNA synthesis kit

1 µg–1 pg total RNA

100 ng–1 pg total RNA

1 µg–1 pg total RNA

2x probes RT-PCR reaction mix (dNTPs,

iTaq DNA polymerase, and stabilizers)

2x SYBR® Green RT-PCR reaction mix (dNTPs, iTaq™ DNA

polymerase, �uorescein,

SYBR® Green I dye, and stabilizers)

5x iScript reaction mix (dNTPs and

buffer components)

iScript reverse transcriptase

(for one-step RT-PCR)


(for one-step RT-PCR)


Fast and easy to use

iScript cDNA synthesis kit

1 µg–100 fg total RNA

5x iScript reaction mix (dNTPs, oligo[dT],

random primers, and buffer components)


cDNA ready in 40 min for qPCR

Maximize data from single

20 μl reaction

7.5 µg–100 fg total RNA

5x iScript advanced reaction mix

(dNTPs, oligo[dT], random primers, and buffer components)



Forward and reverse primers and probe for target gene

(not included)

RT-qPCR data in 60 min

Forward and reverse primers for target gene(not included)

RT-qPCR data in 60–90 min

Oligo(dT), random primers, and gene-speci�c primer

(GSP) enhancer solution (3 vials)

cDNA ready in 40–90 min for qPCR

Reduce pipetting variability

iScript reverse transcription supermix

for RT-qPCR

1 µg–100 fg total RNA

5x iScript RT supermix

(dNTPs, oligo[dT], random primers,

buffer components, and iScript reverse

transcriptase)


iScript advanced cDNA synthesis kit

for RT-qPCR


iScript™ Advanced cDNA Synthesis Kit for RT-qPCR ■■ Increased qPCR data throughput and cost effectiveness from a single 20 µl

reverse transcription (RT) reaction

■■ Superior sensitivity and broad linear dynamic range for RT (7.5 µg–100 fg)

■■ 2-tube kit (5x iScript reaction mix and iScript reverse transcriptase) for ease of use and reduced reaction setup time

■■ Optimized blend of oligo(dT) and random primers ensures complete and unbiased RNA sequence representation

■■ RNase H+ MMLV reverse transcriptase (preblended with RNase inhibitor) delivers high sensitivity for real-time RT-qPCR and eliminates additional RNase H+ step

■■ Potent blend of RNaseA inhibitor protects RNA during setup and RT

■■ Short 35 min protocol allows fast qPCR data generation


ReagentsTwo-Step Reverse Transcription Reagents

iScript Reverse Transcription Supermix for RT-qPCR■■ 1-tube format for simple and fast setup, and reduced pipetting variability

■■ Liquid format at –20°C offers superior stability and eliminates freeze/thaw cycle

■■ Superior sensitivity and broad linear dynamic range for RT (1 µg–100 fg)






iScript reverse transcription supermix for RT-qPCR efficiently reverse transcribes RNA over a broad linear dynamic range for reliable gene expression analysis data. Different amounts of HeLa cell RNA (amounts shown in inset) were reverse transcribed and one-tenth of the resulting cDNA was used as a template to amplify β-actin gene (~90 bp) in 20 µl qPCR reactions with iQ™ SYBR® Green supermix. Standard curve R2 = 0.999, efficiency = 99.7%, slope = –3.33. RFU, relative fluorescence units.

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— 1 µg— 100 ng— 10 ng— 1 ng— 100 pg— 10 pg— 1 pg

Excellent data reproducibility. PGK-1 mRNA (~160 bp), a gene that encodes a glycolytic enzyme, was quantified using iScript reverse transcription supermix for RT-qPCR both with 100 ng () and 100 pg () of input RNA. For each input RNA, 48 individual RT reactions were performed and one-tenth of the resulting cDNA was used in the qPCR reaction with SsoFast™ probes supermix. The gene expression analysis data show excellent reproducibility both with high and low levels of input target mRNA. The ~10 Cq difference for the 1,000-fold dilution of RNA (100 ng–100 pg) demonstrates good reverse transcription efficiencies across different input RNAs. Cq, quantification cycle; RFU, relative fluorescence units.

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RNA Average Cq SD CV, %

100 ng 21.35 0.123 0.576100 pg 31.56 0.147 0.465


iScript advanced cDNA synthesis kit for RT-qPCR provides superior sensitivity and a broad linear dynamic range for reverse transcription. Total RNA (7.5 µg–1 pg) from HeLa cells was reverse transcribed using the iScript advanced cDNA synthesis kit for RT-qPCR in a 20 µl reaction. A tenfold dilution of generated cDNA was used as template to amplify α-tubulin in a 10 µl qPCR reaction with iQ™ SYBR® Green supermix on a CFX384™ real-time PCR detection system. Efficiency = 90.7%, R2 = 0.999, slope = –3.57. Cq, quantification cycle; RFU, relative fluorescence units.

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ReagentsTwo-Step Reverse Transcription Reagents

iScript cDNA Synthesis Kit■■ 2-tube kit (5x iScript reaction mix and iScript reverse transcriptase) for

ease of use and reduced reaction setup time

■■ Superior sensitivity and broad linear dynamic range for RT (1 µg–100 fg)






iScript Select cDNA Synthesis Kit■■ 5-tube kit (random primers, oligo[dT], 5x iScript Select reaction mix, iScript

reverse transcriptase, and gene-specific primer enhancer solution)

■■ Choice of priming strategy

■■ Reliable synthesis of long cDNA >6 kb in length

■■ Superior sensitivity and broad linear dynamic range for RT (1 µg–1 pg)


iScript reagents provide potent RNaseA inhibition. iScript reagents for RT-qPCR include an optimum blend of RNaseA inhibitor for protecting RNA integrity. Reverse transcription was performed using 0.1 pg of input RNA with iScript reagent alone (), spiked with RNaseA (), or spiked with RNaseA without the RNaseA inhibitor included in the reaction (). 18S rRNA (~70 bp) was amplified using iQ™ SYBR® Green supermix. A significant Cq delay was observed when the reaction included RNaseA but no RNaseA inhibitor, which demonstrates potent RNaseA inhibition. RFU, relative fluorescence units.

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The iScript cDNA synthesis kit performs across a broad range of concentrations. Input RNA (amounts shown in inset) was reverse transcribed, and the resulting cDNA was amplified using iQ™ SYBR® Green supermix. Standard curve r2 = 0.998, efficiency = 96.5%. RFU, relative fluorescence units.

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iScript Select cDNA synthesis kit performs reliably over 6 orders of magnitude using a gene-specific primer approach. Human total RNA from 1 μg to 1 pg was reverse transcribed using the iScript Select cDNA synthesis kit. One-tenth of the resulting cDNA was used as a template to amplify β-actin gene with iQ™ SYBR® Green supermix. Standard curve r = 1.000, efficiency = 92.2%. RFU, relative fluorescence units.

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iScript™ One-Step RT-PCR Kit with SYBR® Green■■ For use on a broad range of real-time PCR instruments

■■ Extremely sensitive detection (100 ng–1 pg) of input RNA

iScript One-Step RT-PCR Kit for Probes ■■ For use with all types of hybridization probes, including dual-labeled

oligonucleotide probes, FRET probes, and molecular beacons

■■ Extremely sensitive detection (1 µg–1 pg) of input RNA


Benefits of iScript one-step kits:

■■ Provide powerful combination of iScript RNase H+ reverse transcriptase and antibody-mediated hot-start iTaq™ DNA polymerase

■■ Are ideal for rapid, high-throughput gene expression analysis

■■ Perform cDNA synthesis and qPCR in 1 tube, minimizing handling and contamination risk

iScript™ one-step RT-PCR kit with SYBR® Green provides high reproducibility and sensitivity across a broad range of concentrations. Reactions were performed in triplicate, along with no-template controls, using GAPDH primers and 100 ng–100 fg total HeLa RNA. Reactions were carried out on the iCycler iQ® real-time detection system. Standard curve r = 1.000, efficiency = 95%, slope = –3.47. RFU, relative fluorescence units.

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iScript one-step RT-PCR kit for probes delivers unparalleled results over an extremely wide dynamic range. RNA (1 µg–100 fg) isolated from HeLa cells using the Aurum™ total RNA kit was reverse transcribed and amplified using primers to β-actin and a FAM-labeled detection probe. Each dilution was performed in triplicate and RT-PCR was carried out on the iCycler iQ detection system. Standard curve r = 1.000, efficiency = 97.2%, slope = –3.39. RFU, relative fluorescence units.

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ReagentsOne-Step RT-qPCR Reagents


Catalog # Description $

Two-Step Reverse Transcription Reagents One-Step RT-qPCR Reagents 170-8842 iScript Advanced cDNA Synthesis Kit for RT-qPCR, 50 x 20 μl reactions 170-8892 iScript One-Step RT-PCR Kit with SYBR Green, 50 x 50 μl reactions170-8843 iScript Advanced cDNA Synthesis Kit for RT-qPCR, 250 x 20 μl reactions 170-8893 iScript One-Step RT-PCR Kit with SYBR Green, 200 x 50 μl reactions170-8890 iScript cDNA Synthesis Kit, 25 x 20 μl reactions 170-8894 iScript One-Step RT-PCR Kit for Probes, 50 x 50 μl reactions170-8891 iScript cDNA Synthesis Kit, 100 x 20 μl reactions 170-8895 iScript One-Step RT-PCR Kit for Probes, 200 x 50 μl reactions170-8840 iScript Reverse Transcription Supermix for RT-qPCR, 25 x 20 μl reactions170-8841 iScript Reverse Transcription Supermix for RT-qPCR, 100 x 20 μl reactions 170-8896 iScript Select cDNA Synthesis Kit, 25 x 20 μl reactions170-8897 iScript Select cDNA Synthesis Kit, 100 x 20 μl reactions



http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_3066C.pdf

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Reagents — Real-Time qPCR ■■ Patented Sso7d fusion enzyme technology delivers higher processivity and inhibitor tolerance

■■ Antibody-mediated hot-start polymerases enable instant activation and higher specificity

■■ Choice of fast, standard, or universal cycling conditions

■■ Formulated for optimal performance on a

variety of real-time instruments


Reagents Real-Time qPCR Reagents Selection Guide

SYBR® Green / EvaGreen Supermixes Probes Supermixes One-Step Kits for RT-qPCR

Real-Time PCR Instrument

SsoAdvanced™ SYBR® Green

Supermix

SsoFast™ EvaGreen® Supermix

iQ™ SYBR® Green

Supermix

SsoFast EvaGreen Supermix with Low

ROX

iTaq™ Fast SYBR® Green Supermix with

ROX

iTaq™ SYBR® Green

Supermix with ROX

EpiQ™ Chromatin

SYBR® Green

Supermix

SsoFast Probes

Supermix

iQ Supermix

iQ Multiplex Powermix

SsoFast Probes

Supermixwith ROX

iTaq Fast Supermix with ROX

iTaq Supermix with ROX

iScript™ One-Step

RT-PCR Kit with SYBR®

Green

iScript One-Step

RT-PCR Kit for Probes

Bio-Rad

CFX96™, CFX96 Touch™, CFX384™, CFX384 Touch™, CFX Connect™

✔ ✔ ✔ ✔ ✔ ✔ ✔

✔ ✔

iQ™, iQ™5, MyiQ™, MyiQ™2 ✔ ✔ ✔ ✔ ✔ ✔■ ✔ ✔ ✔

MiniOpticon™, DNA Engine Opticon® I and II ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔

Applied Biosystems

StepOne/StepOne Plus ◆ ◆ ◆ ◆ ◆ ✔ ✔ ◆ ◆ ◆ ✔ ◆ ✔ ◆ ◆

7500, ViiA7 ✔ ✔ ✔ ✔

7000, 7300, 7700, 7900HT ✔ ✔ ✔

Stratagene

Mx3000P, 3005P, 4000 ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔

Eppendorf

Mastercycler ep realplex 2 or 4 ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔

QIAGEN/Corbett

Rotor-Gene 3000, 6000, Q ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔

Roche

LightCycler 480 ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔

LightCycler 1.0, 1.5, 2.0 ▲ ▲ ▲ ▲ ▲ ▲ ✔ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲

Idaho Technology

LightScanner HR-1 ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔

LightScanner 32 ▲ ▲ ▲ ▲ ▲ ▲ ✔ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲

✔ Recommended for use as is ◆ ROX reference setting must be turned “off” ▲ BSA must be added according to instrument specifications


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Sso7d PolymeraseSsoAdvanced™ SYBR® Green Supermix■■ Robust formulation delivers performance across a broad range of standard

and fast cycling conditions

■■ Sso7d fusion polymerase and optimized buffer deliver fast reaction times via instant polymerase activation and rapid polymerization kinetics to generate exceptional qPCR results in less than 30 min

■■ Increased resistance to PCR inhibitors ensures maximum efficiency, sensitivity, and reproducibility, and allows increased fluorescence when compared to other SYBR® Green supermixes

■■ Advanced formulation tolerates a broad range of reaction conditions, primer concentrations, and temperature ranges


Reagents Real-Time qPCR — SYBR® Green/EvaGreen

The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases processivity, and provides greater speed and reduced reaction times compared to conventional DNA polymerases. Sso7d fusion polymerases are significantly more resistant to PCR inhibitors, making the SsoAdvanced and SsoFast supermixes ideal choices for challenging applications, such as direct qPCR, without the need for sample preparation.

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SsoAdvanced™ SYBR® Green supermix provides extreme sensitivity in the detection of a single copy target gene. The cyclin gene was amplified and detected from fivefold serial dilutions of 10 ng–80 pg (■) and 3.2 pg (■) human genomic DNA. Cyclin efficiency = 103%, R2 = 1 (for 10 ng–80 pg). Inset shows the standard curve for the various dilutions. Cq, quantification cycle; RFU, relative fluorescence units.

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Exceptional reproducibility can be achieved with SsoAdvanced™ SYBR® Green supermix. Efficient discrimination and reliable quantification can be obtained from a 1.33-fold serial dilution of input template. The GAPDH gene was amplified from varying amounts of human genomic DNA (1 ng–136 pg). From left to right: 1 ng, 753 pg, 565 pg, 425 pg, 320 pg, 240 pg, 181 pg, and 136 pg. GAPDH efficiency = 96.2%, R2 = 0.999. Inset is a magnified view showing robust discrimination and reproducible amplification (six replicates for each input amount). RFU, relative fluorescence units.

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SsoAdvanced™ SYBR® Green supermix demonstrates superior inhibitor tolerance. The ADAR gene was amplified from HeLa cDNA in the presence of water alone, or in the presence of a known PCR inhibitor, Eagle’s minimal essential medium (EMEM) with fetal bovine serum (FBS; 0, 2.5, 5, 10, and 20%), added to SsoAdvanced™ SYBR® Green supermix (■) or a traditional Taq DNA polymerase–based qPCR master mix (■). SsoAdvanced™ SYBR® Green supermix showed quality amplification in all reactions (EMEM with 20% FBS data shown), while the Taq DNA polymerase–based qPCR master mix failed to amplify in all EMEM with FBS combinations (shown in the inset melt curve). RFU, relative fluorescence units.

www.bio-rad.com/supermixes

http://www.bio-rad.com/supermixes






The unique fusion polymerase in SsoFast EvaGreen supermix with low ROX generates exceptional qPCR results on the ABI 7500 fast real-time PCR system. Tenfold serial dilutions of 10 ng–100 fg cDNA from human spleen were used in each 20 μl reaction to detect α-tubulin. α-tubulin efficiency = 105.8%, r = 0.996. Total qPCR run time = 39 min (not including melt curve).

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SsoFast™ EvaGreen® Supermix■■ Inhibitor tolerance and enhanced processivity with Sso7d fusion polymerase

■■ Robust formulation ensures maximum efficiency, sensitivity, and reproducibility for qPCR

■■ Instant polymerase activation and rapid polymerization kinetics deliver fast qPCR results


SsoFast EvaGreen Supermix with Low ROX ■■ Blended with ROX for optimal performance on Applied Biosystems

standard and fast 7500 real-time PCR instruments



The unique fusion polymerase in SsoFast EvaGreen supermix delivers extreme speed and generates exceptional qPCR results in less than 30 min. Tenfold serial dilutions of 10 ng–100 ag cDNA from human spleen were used in each 20 μl reaction to detect 18S rRNA. 18S rRNA efficiency = 101.8%, r = 0.997. Total qPCR run time = 29 min. RFU, relative fluorescence units.

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SsoFast EvaGreen supermix demonstrates superior inhibitor tolerance and direct qPCR capability from cell culture. Results show efficient amplification and detection of a spike-in control template in the absence (■) or presence (■) of conditioned tissue culture medium using SsoFast EvaGreen supermix. Inset shows a competitor’s standard qPCR reagent is able to amplify the spike-in control template only in the absence (■) vs. presence (■) of culture medium. RFU, relative fluorescence units.

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iQ™ SYBR® Green supermix generates precise, quantitative results. A fivefold dilution series (50 ng–80 pg) of human genomic DNA was amplified using the supermix, primers, and a probe specific to the IL-1β gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ® real-time system. Standard curve r = 0.999, efficiency = 97.6%, slope = –3.38. RFU, relative fluorescence units.

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iTaq™ fast SYBR® Green supermix with ROX generates linear results over 6 orders of magnitude on the ABI 7500 fast real-time PCR system. Tenfold serial dilutions of 100 ng–100 fg cDNA from HeLa total RNA were used in each 20 µl reaction designed to detect β-actin. Efficiency = 93.0%, r = 0.998. Total qPCR run time = 31 min.

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iTaq™ SYBR® Green supermix with ROX generates superior results on the ABI 7900HT system. Total RNA from HeLa cells was reverse transcribed using the iScript™ reverse transcription supermix for RT-qPCR. A tenfold dilution of generated cDNA (10 ng–100 fg) was used as template to amplify the β-actin gene. Standard curve R2 = 0.999, efficiency = 95.7%, slope = –3.43.

iQ™ SYBR® Green Supermix■■ Analysis of low-, medium-, and high-abundance target genes with superior

sensitivity and efficiency

■■ Formulated for maximum SYBR® Green I stability and performance in a wide variety of real-time PCR instruments

■■ Antibody-mediated hot-start polymerase for quick activation and increased specificity


iTaq™ Fast SYBR® Green Supermix with ROX■■ Developed and validated for Applied Biosystems standard and fast 7500

and Stratagene real-time PCR instruments

■■ Compatible with standard and fast thermal cycling protocols


iTaq™ SYBR® Green Supermix with ROX■■ Developed and validated for Applied Biosystems 7000, 7300, 7700,

7900, StepOne, and StepOne Plus real-time PCR instruments

■■ Increased specificity and reduced primer-dimers with antibody-mediated hot-start polymerase


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http://www.bio-rad.com/webroot/web/pdf/lsr/literature/bulletin_2764E.pdf





Precision Melt Supermix■■ Optimized formulation containing EvaGreen dye delivers robust PCR and

high resolution melt (HRM) performance

■■ Sensitive and effective discrimination of all 4 SNP classes across a broad range of amplicons

■■ Accurate detection of CpG methylation status for epigenetic studies

■■ Exceptional room temperature stability for high-throughput HRM studies

■■ Reliable performance on any HRM-capable thermal cycler



EpiQ™ Chromatin SYBR® Green Supermix■■ Robust formulation delivers superior sensitivity and efficiency for qPCR from

genomic DNA templates

■■ Protocol is optimized for difficult real-time qPCR reactions for high GC amplicons

■■ Supermix contains fluorescein and ROX and is compatible with all real-time PCR instruments except Applied Biosystems 7000, 7300, 7700, and 7900 models (additional ROX can be added by ordering the dye separately, catalog #172-5858)

Please refer to page 22 for more details about the EpiQ chromatin analysis kit.


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–0.04

–0.06

–0.08

Diff

eren

ce R

FU

75 76 77 78 79 80Temperature, °C

Precision melt supermix delivers robust HRM for single nucleotide polymorphisms (SNPs). Discrimination of class I and IV SNP genotypes are shown in panels A and B, respectively. Class I (A to G substitution) and class IV (A to T substitution) SNP genotypes from mouse genomic DNA were analyzed using precision melt supermix. Wild type (■), heterozygote (■), and homozygous mutant (■) are shown in the difference plots normalized to wild-type samples. HRM analysis was performed on a CFX384™ real-time PCR detection system and genotypes were automatically assigned by Precision Melt Analysis™ software. Amplification was carried out for 35 cycles. Total run time including melt curve = 150 min. RFU, relative fluorescence units.

Accurate methylation detection with precision melt supermix. Mixtures of methylated and unmethylated human genomic DNA of varying ratios were analyzed using HRM on a CFX384 real-time PCR detection system. Increasing amounts of methylated DNA (■, 0%; ■, 2%; ■, 5%; ■, 50%; ■, 75%; ■, 95%; and ■, 100%) were analyzed for methylation of the human RARB2 gene. The genomic region contains 7 CpG sites and is 88 base pairs in length. Total run time including melt curve = 190 min. RFU, relative fluorescence units.






Reag

ents

iQ™ Supermix ■■ Maximum efficiency and sensitivity for qPCR using fluorogenic probes

■■ Reliable amplification over a wide dynamic range of human genomic and plasmid DNA concentrations

■■ Contains antibody-mediated hot-start iTaq™ DNA polymerase for quick activation and increased specificity


Reagents Real-Time qPCR Probes

1,000

100

0 5 10 15 20 25 30 35 40 45 50Cycles

RFU

iQ supermix provides sensitive real-time detection over 8 orders of magnitude. Tenfold dilutions of a plasmid containing 109–10 copies of the α-tubulin gene were amplified using iQ supermix and a FAM-labeled hybridization probe for detection. Eight replicates at each concentration were amplified along with no-template controls on the MyiQ™ real-time PCR detection system. Standard curve r = 0.999, efficiency = 98.2%, slope = –3.36. RFU, relative fluorescence units.

RFU

104

103

102

101

0 10 20 30 40Cycles

log starting quantity

Standard CurveC

q

Exceptional reproducibility can be achieved on the CFX384 real-time PCR detection system with SsoFast probes supermix. Efficient discrimination and reliable quantification can be obtained from 1.33-fold serial dilutions of input template. The GAPDH gene was amplified from varying amounts of HeLa cDNA (1 ng–102 pg). From left to right: () 1 ng, 565 pg, 320 pg, 181 pg, and 102 pg; () 752 pg, 425 pg, 240 pg, and 136 pg. GAPDH efficiency = 91.5%, R2 = 0.997. Inset shows the standard curve for the various dilutions. Total qPCR run time = 50 min. Cq, quantification cycle; RFU, relative fluorescence units.

SsoFast™ Probes Supermix■■ Robust, simultaneous detection of up to 2 different gene targets using

fluorogenic probes

■■ Compatible with fast or standard cycling protocols

■■ Inhibitor-tolerant polymerase suitable for analyzing difficult samples


SsoFast Probes Supermix with ROX ■■ Developed and validated for Applied Biosystems standard and fast

7900HT real-time PCR instruments


SsoFast probes supermix delivers superior results for gene expression analysis of two targets on the CFX96™ real-time PCR detection system, with no difference in detection of a low-expressing gene in duplex or singleplex. cDNA from human liver (100 ng) was used in each 20 µl reaction. (■) HEX-labeled GAPDH probe duplex reaction; (■) Texas Red–labeled IL-2 probe duplex reaction; (■) HEX-labeled GAPDH probe simplex reaction; (■) Texas Red–labeled IL-2 probe simplex reaction. Total qPCR run time = 38 min. RFU, relative fluorescence units.

RFU

105

104

103

102

0 10 20 30 40Cycles


http://www.bio-rad.com/webroot/web/pdf/lsr/literature/bulletin_2764E.pdf





Reagents Real-Time qPCR Probes

1,000

100

0 5 10 15 20 25 30 35 40 45Cycles

PC

R b

asel

ine-

subt

ract

ed c

urve

fit,

RFU

iQ multiplex powermix produces highly reliable qPCR results for up to five targets in a single tube, with no difference in detection of a low-expressing gene in multiplex or singleplex. One-tenth of a 1 µg cDNA synthesis reaction of human thymus total RNA was used in each 20 µl reaction. FAM-labeled β-actin probe (), Cy5-labeled α-tubulin probe (), HEX-labeled GAPDH probe (), TAMRA-labeled cyclophilin probe (), Texas Red–labeled IL-2 probe (). RFU, relative fluorescence units.

iTaq supermix with ROX produces superior results on the ABI 7900HT system. Total RNA from HeLa cells was reverse transcribed using the iScript™ reverse transcription supermix for RT-qPCR. A tenfold dilution of generated cDNA (100 ng–1 pg) was used as template to amplify the β-actin gene with a FAM-labeled probe. Standard curve R2 = 0.999, efficiency = 98.0%, slope = –3.38.

10.000

1.000

0.100

∆R

n

0 5 10 15 20 25 30 35 40 45Cycles

Robust duplex qPCR results with iTaq fast supermix with ROX on the Applied Biosystems 7500 fast real-time PCR system. cDNA inputs: 100-fold serial dilutions of cDNA from the equivalent of 1 µg–1 pg total RNA were used in each 20 µl reaction. FAM-labeled 18S rRNA probe duplex reaction (), VIC-labeled beta-2-microglobulin (B2M) probe duplex reaction (), VIC-labeled B2M probe singleplex reaction (). 18S rRNA efficiency = 98.6%, r = 0.999; B2M efficiency = 98.0%, r = 0.998. Total qPCR run time = 45 min.

iQ™ Multiplex Powermix ■■ Robust supermix formulated for sensitive and efficient multiplex qPCR

■■ Reliable quantification of up to 4 targets (expression levels can vary up to 106-fold between target genes) or up to 5 targets

■■ Linearity over 6 orders of magnitude of input cDNA and 4 orders of magnitude of input genomic DNA

■■ Suitable for a wide variety of applications, including gene expression analysis, SNP genotyping, SNP analysis, GMO detection, and viral load detection


iTaq™ Fast Supermix with ROX■■ Developed and validated for Applied Biosystems 7500 standard and fast

and Stratagene real-time PCR instruments

■■ Compatible with standard and fast thermal cycling conditions

■■ Antibody-mediated iTaq DNA polymerase provided in a convenient 2x supermix


iTaq Supermix with ROX■■ Developed and validated for Applied Biosystems 7000, 7300, 7700, 7900,

StepOne, and StepOne Plus real-time PCR instruments

■■ Sensitive and accurate detection over 6 orders of magnitude

■■ Hot-start iTaq DNA polymerase in optimized buffer for simplex and duplex qPCR


1.000

0.100

∆R

n

0 10 20 30 40Cycles







Reag

ents

iProof™ High-Fidelity DNA Polymerase ■■ A high-fidelity DNA polymerase with 52-fold more accuracy than Taq

DNA polymerase

■■ Unique Pyrococcus-like proofreading enzyme is fused to a dsDNA binding protein, Sso7d

■■ Long and fast PCR applications — fragments up to 37 kb are amplified in less time (15–30 sec/kb) and with less enzyme (0.25–1 U/reaction)

■■ Convenient 2x supermix is available for iProof polymerase and buffer for GC-rich templates


dNTP Mix ■■ Formulated for consistency and higher efficiency in PCR and real-time PCR

■■ Robust dNTP solution withstands multiple rounds of freeze-thawing and temperature cycling

iTaq DNA Polymerase ■■ Antibody-mediated hot-start DNA polymerase for quick 3 min activation

at 95°C

■■ Polymerase prevents nonspecific amplification and primer-dimers in both PCR and real-time PCR applications


iProof high-fidelity DNA polymerase demonstrates unrivaled speed, leading to dramatically shorter overall reaction times. The reaction protocol for iProof polymerase was compared to the recommended protocols for two competing polymerases. Each protocol was designed to amplify 1, 8, and 15 kb products in 30 cycles. Reactions with iProof polymerase used a two-step protocol with a combined annealing and extension step, while the other reactions used three-step protocols with the minimum recommended extension times. Overall reaction times include temperature ramping times.

iProof high-fidelity DNA polymerase amplifies long templates with high yields. Left, various fragments up to 37 kb in length were amplified from BAC DNA using a combined annealing/extension step of 10 min per cycle and 30 U/ml of iProof polymerase. Right, various sequences up to 28.8 kb were amplified directly from human genomic DNA using 30 U/ml of iProof polymerase in GC buffer with a combined annealing/extension time of 10 min per cycle.

1 kb35 min

2 hr2 hr

8 kb1.5 hr

9.5 hr5.5 hr

15 kb2 hr 20 min

16.5 hr(Failed amplification)

Total protocol time

Tem

plat

e le

ngth

iProof high-fidelity DNA polymerase

Common high-fidelity polymerase

Taq polymerase

5

10 20 22 25 32 37

2.9

3.8

7.9

15 22 28 – 48 kb

– 23 kb

BAC DNA (37 kb) Human genomic DNA (28 kb)

Reagents Standard PCR

www.bio-rad.com/pcrreagents


http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_2779E.pdf

http://www.bio-rad.com/pcrreagents


Reagents Ordering Information


Catalog # Description $

qPCR Supermixes with SYBR Green or EvaGreen 172-5260 SsoAdvanced SYBR Green Supermix, 200 x 20 µl reactions172-5261 SsoAdvanced SYBR Green Supermix, 500 x 20 µl reactions172-5262 SsoAdvanced SYBR Green Supermix, 1,000 x 20 µl reactions172-5264 SsoAdvanced SYBR Green Supermix, 2,500 x 20 µl reactions172-5265 SsoAdvanced SYBR Green Supermix, 5,000 x 20 µl reactions172-5200 SsoFast EvaGreen Supermix, 200 x 20 µl reactions 172-5201 SsoFast EvaGreen Supermix, 500 x 20 µl reactions 172-5202 SsoFast EvaGreen Supermix, 1,000 x 20 µl reactions 172-5203 SsoFast EvaGreen Supermix, 2,000 x 20 µl reactions 172-5204 SsoFast EvaGreen Supermix, 2,500 x 20 µl reactions 172-5205 SsoFast EvaGreen Supermix, 5,000 x 20 µl reactions 172-5210 SsoFast EvaGreen Supermix with Low ROX, 200 x 20 µl reactions 172-5211 SsoFast EvaGreen Supermix with Low ROX, 500 x 20 µl reactions 172-5212 SsoFast EvaGreen Supermix with Low ROX, 1,000 x 20 µl reactions 172-5213 SsoFast EvaGreen Supermix with Low ROX, 2,000 x 20 µl reactions 170-8880 iQ SYBR Green Supermix, 100 x 50 µl reactions 170-8882 iQ SYBR Green Supermix, 500 x 50 µl reactions 170-8884 iQ SYBR Green Supermix, 1,000 x 50 µl reactions 170-8885 iQ SYBR Green Supermix, 2,000 x 50 µl reactions 170-8886 iQ SYBR Green Supermix, 2,500 x 50 µl reactions 170-8887 iQ SYBR Green Supermix, 5,000 x 50 µl reactions 172-5100 iTaq Fast SYBR Green Supermix with ROX, 200 x 20 µl reactions 172-5101 iTaq Fast SYBR Green Supermix with ROX, 500 x 20 µl reactions 172-5102 iTaq Fast SYBR Green Supermix with ROX, 1,000 x 20 µl reactions 172-5103 iTaq Fast SYBR Green Supermix with ROX, 2,000 x 20 µl reactions 172-5850 iTaq SYBR Green Supermix with ROX, 200 x 50 µl reactions 172-5851 iTaq SYBR Green Supermix with ROX, 500 x 50 µl reactions 172-5852 iTaq SYBR Green Supermix with ROX, 1,000 x 50 µl reactions 172-5853 iTaq SYBR Green Supermix with ROX, 2,000 x 50 µl reactions 172-5110 Precision Melt Supermix, 200 x 20 µl reactions172-5112 Precision Melt Supermix, 1,000 x 20 µl reactions172-5404 EpiQ Chromatin SYBR Green Supermix, 500 x 20 µl reactions172-5405 EpiQ Chromatin SYBR Green Supermix, 1,000 x 20 µl reactions

qPCR Supermixes for Probes172-5230 SsoFast Probes Supermix, 200 x 20 µl reactions 172-5231 SsoFast Probes Supermix, 500 x 20 µl reactions 172-5232 SsoFast Probes Supermix, 1,000 x 20 µl reactions 172-5233 SsoFast Probes Supermix, 2,000 x 20 µl reactions 172-5250 SsoFast Probes Supermix with ROX, 200 x 20 µl reactions 172-5251 SsoFast Probes Supermix with ROX, 500 x 20 µl reactions 172-5252 SsoFast Probes Supermix with ROX, 1,000 x 20 µl reactions 172-5253 SsoFast Probes Supermix with ROX, 2,000 x 20 µl reactions 170-8860 iQ Supermix, 100 x 50 µl reactions 170-8862 iQ Supermix, 500 x 50 µl reactions 170-8864 iQ Supermix, 1,000 x 50 µl reactions 172-5848 iQ Multiplex Powermix, 50 x 50 µl reactions 172-5849 iQ Multiplex Powermix, 200 x 50 µl reactions 172-5105 iTaq Fast Supermix with ROX, 200 x 20 µl reactions 172-5106 iTaq Fast Supermix with ROX, 500 x 20 µl reactions 172-5107 iTaq Fast Supermix with ROX, 1,000 x 20 µl reactions 172-5108 iTaq Fast Supermix with ROX, 2,000 x 20 µl reactions 172-5854 iTaq Supermix with ROX, 200 x 50 µl reactions 172-5855 iTaq Supermix with ROX, 500 x 50 µl reactions 172-5856 iTaq Supermix with ROX, 1,000 x 50 µl reactions 172-5857 iTaq Supermix with ROX, 2,000 x 50 µl reactions Standard PCR Supermixes 172-5300 iProof High-Fidelity DNA Polymerase, 2 U/µl, 20 U 172-5301 iProof High-Fidelity DNA Polymerase, 2 U/µl, 100 U 172-5302 iProof High-Fidelity DNA Polymerase, 2 U/µl, 500 U 172-5310 iProof HF Master Mix, 0.04 U/µl, 100 x 50 µl reactions172-5311 iProof HF Master Mix, 0.04 U/µl, 500 x 50 µl reactions172-5320 iProof GC Master Mix, 0.04 U/µl, 100 x 50 µl reactions172-5321 iProof GC Master Mix, 0.04 U/µl, 500 x 50 µl reactions170-8870 iTaq DNA Polymerase, 5 U/µl, 250 U 170-8875 iTaq DNA Polymerase, 5 U/µl, 5,000 U 170-8874 dNTP Mix, 200 µl 172-5858 ROX Passive Reference Dye, 0.5 ml


Reag

ents

Chromatin Analysis

EpiQ™ Chromatin Analysis Kit ■■ Novel assay generates quantitative chromatin structure information with

strong correlation to gene expression levels

■■ Quantitative assessment of chromatin structure of target genes in cultured cells

■■ Kit discriminates open, actively transcribed chromatin regions from closed, transcriptionally silent regions

■■ In situ chromatin digestion, genomic DNA purification, and real-time PCR all combined in one workflow

■■ Short assay time — assessment of chromatin structure can be accomplished in less than 6 hr

■■ Small sample requirement — as little as 5 x 104 cells are required to perform analysis


The EpiQ chromatin analysis kit utilizes nuclease accessibility to discriminate open vs. closed chromatin regions. Amplification of proximal promoter regions for the epigenetically silenced HBB (reference) gene or the constitutively expressed GAPDH (target) gene was carried out in HeLa cells using the EpiQ kit and EpiQ™ chromatin SYBR® Green supermix on the CFX96™ real-time PCR detection system. A, closed chromatin regions were protected from nuclease digestion and remained intact prior to amplification, resulting in minimal quantification cycle (Cq) delays (∆Cq = 0.58) following nuclease treatment; B, open chromatin regions were susceptible to nuclease digestion and were unavailable for amplification, leading to significant Cq delays (∆Cq = 8.08) after nuclease treatment. A comparison of ∆Cqs with the amplification efficiencies for each gene target factored in was used to determine the accessibility of the target gene, calculated to be >99% for GAPDH. RFU, relative fluorescence units.

Chromatin consists of DNA spooled around complexes of histone protein molecules called nucleosomes ().

EpiQ Chromatin Analysis Kit


Catalog # Description

172-5400 EpiQ Chromatin Analysis Kit, 50 preparations 172-5401 EpiQ Chromatin Analysis Kit, 100 preparationsKit components and supermixes can be purchased separately. For more information, visit www.bio-rad.com/epiq.

RFU

104

103

102

101

0 10 20 30 40Cycles

— No nuclease— With nuclease

∆Cq target = 8.08

RFU

104

103

102

101

0 10 20 30 40Cycles

— No nuclease— With nuclease

∆Cq ref = 0.58

A. HBB — Reference Gene (epigenetically silenced) B. GAPDH — Target Gene (constitutively expressed)

www.bio-rad.com/epiq


http://www.bio-rad.com/epiq

PCR Plastic Consumables ■■ Precisely manufactured for optimal fit and cycling performance

■■ Produced in Class 10,000 or 100,000 cleanroom environment

■■ Certified to be free of DNase, RNase, and human genomic DNA

■■ Extremely uniform wells reduce well-to-well variability in real-time PCR

■■ Warp-free Hard-Shell® plates are designed for optimum performance with automation


Plastics

PCR Plastic Consumables Instrument Compatibility

0.2 ml Tubes 384-Well Plates

Individual High-Profile Strips High-Profile Strips Low-Profile Hard-Shell® Standard Hard-Shell 480

Catalog # TBI-0201, TFI-0201, TWI-0201 TBS-xxxx, TBC-xxxx TLS-xxxx HSP-3xxx HSR-48xx

Thermal Cycler Bio-Rad C1000™, C1000 Touch™, S1000™ ✔ ▲ ✔ ✔ ▲

Bio-Rad DNA Engine®, DNA Engine Tetrad®, DNA Engine Tetrad 2, ✔ ▲ ✔ ✔ ▲ DNA Engine Dyad®, Dyad Disciple™, PTC-100®

Bio-Rad T100™, MyCycler™ ✔ ✔

Bio-Rad iCycler® ✔ ✔

Bio-Rad MJ Mini™ ✔ ▲ ✔

Applied Biosystems 0.2 ml tube ✔ ✔

cyclers (2720, 9700, Veriti)

Applied Biosystems 0.1 ml tube ✔

cyclers (9800 fast, Veriti fast)

Applied Biosystems 384-well ✔ ▲ cyclers (9700, Veriti)

Eppendorf Mastercycler series ✔ ▲ ✔ ✔ ▲

Real-Time PCR Instrument Bio-Rad CFX96™, CFX96 Touch™, CFX384™,*

✔ ✔ ▲

CFX384 Touch™,* CFX Connect™

Bio-Rad iCycler iQ®, iQ™5, MyiQ™, MyiQ™2 ✔

Bio-Rad Chromo4™ ▲ ✔

Bio-Rad DNA Engine Opticon® and Opticon 2 ✔

Bio-Rad MiniOpticon™* ✔

Applied Biosystems standard ✔ ✔ ▲ systems (7300, 7500, 7900HT)

Applied Biosystems fast systems (7500 fast, 7900HT fast, StepOne, ✔ ✔ ▲ StepOnePlus)

Eppendorf Mastercycler ep realplex ▲ ✔

Stratagene (Agilent) Mx series ✔

QIAGEN/Corbett Rotor-Gene ✔

Roche LightCycler 480 ✔ Other Instruments Applied Biosystems DNA

✔

sequencers (3100, 3700, 3730)

Idaho Technology LightScanner ✔

✔ Recommended ▲ Compatible


48- and 96-Well Plates

Hard-Shell® Semi-Skirted Hard-Shell Skirted Multiplate™ Unskirted Multiplate Unskirted iQ™ Semi-Skirted High-Profile Low-Profile High-Profile Low-Profile High-Profile

Catalog # HSS-xxxx HSP-9xxx MLP-xxxx MLL-xxxx 223-9441

Thermal Cycler Bio-Rad C1000™, C1000 Touch™,

▲ ✔ ▲ ▲ ▲ S1000™

Bio-Rad T100™ ✔ ▲ ▲

Bio-Rad DNA Engine®, DNA Engine Tetrad®, DNA Engine Tetrad 2, ▲ ✔ ▲ ▲ ▲ DNA Engine Dyad®, Dyad Disciple™, PTC-100®

Bio-Rad MyCycler™ ✔ ▲

Bio-Rad iCycler® ▲ ✔ ▲

Bio-Rad MJ Mini™ ▲ ✔

Applied Biosystems 0.2 ml tube cyclers ✔ ▲ ▲ (2720, 9700, Veriti)

Applied Biosystems 0.1 ml tube cyclers ✔ (9800 fast, Veriti fast)

Eppendorf Mastercycler series ▲ ✔ ▲ ▲ ▲ Real-Time PCR Instrument

Bio-Rad CFX96™, CFX96 Touch™, ✔ ▲

CFX Connect™

Bio-Rad iCycler iQ®, iQ™5, MyiQ™, ▲ ▲ ✔

MyiQ™2

Bio-Rad Chromo4™ ▲ ✔ ▲ ▲ ▲

Bio-Rad DNA Engine ✔ ▲

Opticon®, Opticon 2

Bio-Rad MiniOpticon™* ▲

Applied Biosystems standard ✔

▲

▲

systems (7300, 7500, 7900HT) Except 7900HT Except 7900HT

Applied Biosystems fast systems ▲ (7500 fast, StepOne, StepOnePlus) Except 7900 fast

Eppendorf Mastercycler ep realplex ▲ ✔ ▲ ▲ ▲ Stratagene (Agilent) Mx series ✔ ▲ ▲

Other Instruments Applied Biosystems DNA

✔ ▲

sequencers (3100, 3700, 3730)

Idaho Technology LightScanner ▲ ✔ ▲ ▲

✔ Recommended ▲ Compatible* CFX384™, CFX384 Touch™, and MiniOpticon real-time PCR detection systems are factory calibrated for white tubes and white-well plates. White plastics are recommended due to their superior

signal-to-noise ratio. Using clear tubes or clear-well plates on these instruments will require user calibration.

PCR Plastic Consumables Instrument Compatibility


Plastics

TLS-0801, clearTLS-0851, whiteLow-Profile Tube Strips without Caps

TBS-0201, 8-tube, clearTBS-1201, 12-tube, clearHigh-Profile Tube Strips without Caps

Individual PCR Tubes, 0.2 and 0.5 mlThese high-profile PCR tubes have double-locking caps that won’t pop open during cycling. PCR volume ranges are 5–125 µl for 0.2 ml tubes and 10–200 µl for 0.5 ml tubes. Tubes with flat, frosted caps for easy labeling are available in both 0.2 and 0.5 ml sizes (not suitable for real-time PCR).

To help prevent accidental contamination by multiple users, the 0.5 ml individual tubes with attached caps are available in resealable plastic bags of 100 tubes. Use of a capping tool is recommended for proper sealing of caps on tubes.

Both tubes and caps are available in strips of 8 or 12 for use in 48-well and 96-well sample blocks.

■■ Tight sealing and convenient handling for multiple samples■■ Choice of domed or flat optical cap strips

TWI-0201, 0.2 ml, domed cap

TBI-0501, TBI-0502,0.5 ml, flat cap

TFI-0201,0.2 ml, flat cap

TBI-0201, 0.2 ml, without cap

High-Profile 0.2 ml PCR Tube StripsRecommended reaction volumes are 5–125 µl, which are suitable for most standard PCR and qPCR instruments.

Low-Profile 0.2 ml PCR Tube Strips These tubes reduce the potential for condensate formation and also allow greater light capture in fluorescence assays, such as those performed in real-time PCR. Low-profile tubes are ideal for use in fast and low-volume PCR reactions. The tube height is 15.5 mm. Low-profile tubes are available in opaque white for optical applications.

Cap Strips for 0.2 ml PCR Tubes and PCR PlatesThese cap strips provide extremely tight sealing of all Bio-Rad 0.2 ml PCR tubes and plates during thermal cycling and cold storage. Flat cap strips feature ultraclear upper surfaces, which are ideal for fluorescence applications. Average light transmittance is 1.7-fold higher than with standard-clarity domed cap strips. Flat caps are available in strips of 8 and domed caps are available in strips of 8 or 12. Use of a capping tool is recommended for proper sealing of caps on tubes or plates.

TCS-0803, ultraclearOptical Flat 8-Cap Strips

TCS-0801, 8-cap, clearTCS-1201, 12-cap, clearDomed Cap Strips

PCR Plastic Consumables Tubes and Strips

Individual Tubes

www.bio-rad.com/pcrplastics

http://www.bio-rad.com/pcrplastics


Capping Tools and Racks Multiplate 48-Well PCR PlatesThe versatile, unskirted design and 48-well format make these Multiplate unskirted PCR plates ideal for laboratories using 48-well blocks on Bio-Rad instruments. The plates are suitable for reaction volumes of 5–125 µl. The polypropylene construction of Multiplate PCR plates confers very low protein binding and excellent preservation of sample volume. When less than a full plate is needed, these plates can be easily cut with scissors to the required size. Two plate styles are available:

■■ High-profile (20.70 mm) wells, clear — designed to fit in most thermal cyclers

■■ Low-profile (15.50 mm) wells, clear or white — optimized for fast PCR and low-volume reactions

Compatible Instruments

Bio-Rad C1000™, C1000 Touch™, T100™ (high-profile plates only), S1000™, DNA Engine® family, MJ Mini™, MiniOpticon™ (low-profile white plates recommended)

MLP-4801, clearMultiplate 48-Well Unskirted PCR Plates

MLL-4801, clear MLL-4851, whiteMultiplate Low-Profile 48-Well Unskirted PCR Plates

TRC-9601, ANSI/SBS standardPCR Tube Rack

ECT-1000Easy Cap™ Tool

ECT-2000Strip Cap Tool

TRC-0501, with covers96-Place Racks

PCR Plastic Consumables Capping Tools, Racks, and Multiplate™ Plates




Plastics

Features of Hard-Shell PCR plates include:

■■ Uniform wells that reduce well-to-well variability in optical assays, such as those performed in real-time PCR

■■ White-well option, which allows increased fluorescent signal strength

■■ Color-coded skirts with clear or white wells

■■ Low-cost, user-readable bar code option for convenient database tracking

Hard-Shell Low-Profile 96-Well Skirted PCR Plates Features of the skirted plates include:

■■ Reaction volumes of 5–125 µl (200 µl maximum)■■ Low-profile (16.05 mm) wells optimized for low-volume reactions and fast PCR■■ Superior stability and flatness, allowing precise positioning for automation■■ Full skirt for robotic handling and labeling surface■■ Footprint and well spacing that match ANSI/SBS standard dimensions■■ Black alphanumeric labeling for easy well identification


Bio-Rad C1000, C1000 Touch, S1000, DNA Engine family, PTC-100®, CFX96™, CFX96 Touch™, CFX Connect™, Chromo4™, DNA Engine Opticon®; Eppendorf Mastercycler series; Idaho Technology LightScanner

Hard-Shell TechnologyHard-Shell PCR plates are specifically designed to withstand the stresses of heat sealing, thermal cycling, and robotic handling. The patented two-component design features a skirt and deck molded from a rigid, thermostable polymer. In a separate step, the thin-wall wells are molded of virgin polypropylene selected for low DNA binding. This design prevents problems due to the warping, shrinkage, and sticking that may occur when single-component polypropylene PCR plates are exposed to the high temperatures of thermal cycling or heat sealing. Thus, performance is improved in many applications. In addition, these plates can withstand –80°C storage and high centrifugation forces, making them convenient for alcohol precipitations.


HSP-9601, white shell, clear wellHSP-9655, white shell, white wellOther colors and bar code option availableHard-Shell Low-Profile 96-Well Skirted PCR Plates

The skirt and deck of a Hard-Shell plate prevent warping and shrinkage

Thin-wall polypropylene V-shaped wells enable optimal thermal transfer and recovery of low-volume samples

Raised rims allow for tight sealing

PCR Plastic Consumables Hard-Shell® Plates





Hard-Shell High-Profile 96-Well Semi-Skirted PCR Plates These Hard-Shell PCR plates fit most thermal cyclers. Features of semi-skirted plates include:

■■ Reaction volumes of 5–125 µl (350 µl maximum)

■■ High-profile (20.75 mm) wells that fit most thermal cyclers, real-time PCR detection systems, and DNA sequencers

■■ Warp-free half-height skirt for improved robotic handling and labeling surface

■■ Black alphanumeric labeling for easy well identification


Bio-Rad C1000™, C1000 Touch™, S1000™, T100™, DNA Engine® family, PTC-100®, iCycler®, iQ™5, iCycler iQ®, MyiQ™, MyiQ™2, Chromo4™; Applied Biosystems 0.2 ml tube cyclers, real-time systems, and DNA sequencers; Eppendorf Mastercycler series; Stratagene (Agilent) Mx series; Idaho Technology LightScanner

Multiplate 96-Well Unskirted PCR PlatesThe single-component polypropylene construction of Multiplate PCR plates confers very low protein binding and excellent retention of sample. When less than a full plate is needed, these plates are easily cut with scissors to the required size. Two plate styles are available.

High-profile for most standard instruments

HSS-9601, clear shell, clear wellHSS-9641, green shell, clear wellHSS-9901, clear shell, clear well, bar-codedHSS-9665, black shell, white wellHard-Shell High-Profile 96-Well Semi-Skirted PCR Plates

MLP-9601, clearMLP-9651, whiteMultiplate 96-Well Unskirted PCR Plates

MLL-9601, clearMLL-9651, whiteMultiplate Low-Profile 96-Well Unskirted PCR Plates

Low-profile for most fast instruments


Bio-Rad C1000, C1000 Touch, S1000, DNA Engine family, PTC-100, MJ Mini, CFX96™, CFX96 Touch™, CFX Connect™, Chromo4, DNA Engine Opticon®; Applied Biosystems 0.1 ml tube fast cyclers and real-time systems; Eppendorf Mastercycler series; Idaho Technology LightScanner


Bio-Rad C1000, C1000 Touch, S1000, T100, DNA Engine family, PTC-100, MyCycler™, iCycler, MJ Mini™, iQ5, iCycler iQ, MyiQ, MyiQ2, Chromo4, MiniOpticon™; Applied Biosystems 0.2 ml tube cyclers, real-time systems, and DNA sequencers; Eppendorf Mastercycler series; Stratagene (Agilent) Mx series; Idaho Technology LightScanner

PCR Plastic Consumables Hard-Shell® and Multiplate™ Plates




Plastics

Hard-Shell 384-Well PCR PlatesHard-Shell PCR plates are designed to withstand the stresses of thermal cycling and robotic handling. The patented two-component design provides superior stability and flatness, allowing precise positioning for automation. Features include:

■■ Reaction volumes of 1–30 µl (50 µl maximum)

■■ Extremely uniform wells that reduce well-to-well variability in optical assays, such as those performed in real-time PCR

■■ White-well option for increased fluorescent signal strength

■■ Color-coded skirts with clear or white wells

■■ Warp-free skirt and deck for improved robotic handling

■■ Footprint and well spacing that match ANSI/SBS standard dimensions

■■ Low-cost, user-readable bar code option for convenient database tracking

Two plate styles are available.

Standard for most instruments

480 for Roche LightCycler 480


Roche LightCycler 480; Bio-Rad C1000, C1000 Touch, S1000, DNA Engine family, CFX384, CFX384 Touch; Applied Biosystems cyclers and real-time systems; Eppendorf Mastercycler series

iQ High-Profile 96-Well Semi-Skirted Real-Time PCR Plates These semi-skirted, high-profile PCR plates are optimized for iQ5, iCycler iQ, MyiQ2, and MyiQ real-time PCR detection systems. The semi-skirted design adds stiffness and a labeling surface. Plates are perforated every three columns for easy setup of triplicate reactions.


Bio-Rad C1000, C1000 Touch, S1000, T100, DNA Engine family, PTC-100, MyCycler, iCycler, MyiQ, iCycler iQ, iQ5, MyiQ2, Chromo4; Applied Biosystems 0.2 ml tube cyclers and real-time systems; Eppendorf Mastercycler series; Stratagene (Agilent) Mx series

223-9441iQ 96-Well PCR Plates

HSP-3801, clear shell, clear wellHSP-3805, clear shell, white wellOther colors and bar code option available Hard-Shell 384-Well Standard PCR Plates

HSR-4805K, clear shell, white well, 100 plates and 100 seals HSR-4801K, clear shell, clear well, 100 plates and 100 sealsHard-Shell 384-Well 480 PCR Plates


Bio-Rad C1000, C1000 Touch, S1000, DNA Engine family, CFX384™, CFX384 Touch™; Applied Biosystems cyclers, real-time systems, and DNA sequencers; Eppendorf Mastercycler series; Idaho Technology LightScanner

PCR Plastic Consumables IQ™ and Hard-Shell Plates




Microseal 'C' Optical SealsMicroseal 'C' optical seals are optically clear adhesive films designed for optical assays, such as those performed in real-time PCR. The seals can be used for microplates with raised well rims. Features include:

■■ Form a tight seal for PCR and qPCR■■ Pressure- and heat-sensitive adhesive sticks to the plate, not your gloves■■ Sealing tool for applying even pressure is included■■ Free from DNase, RNase, and human DNA contaminants

Microseal 'B' Adhesive Seals, Optically ClearMicroseal 'B' seals provide an adhesive-based sealing option for thermal cycling using thin-wall PCR plates. The strong adhesive layer ensures secure sample storage (from as low as –40°C and up to 110°C) or transport before or after cycling, as well as tight sealing during thermal cycling when supplemental pressure is applied by a heated lid. This clear polyester film allows easy inspection of sample wells and effective light transmission for optical assays. The sealing surface is protected from contamination by a peel-away release liner. Perforated end-tabs allow removal of overhanging film for automation and other applications. Accessories include:

■■ Optical compression pad (96-well) — enhances the seal integrity of Microseal 'B' clear seals when used in real-time PCR detection systems

■■ Optical film sealing kit — contains 100 Microseal 'B' clear seals and an optical compression pad

MSB-1001Microseal 'B' Adhesive Seals

MSA-5001Microseal 'A' Film

MSR-0001Sealing Roller

ADR-3296Optical Compression Pad

Microseal 'F' FoilThese aluminized foil seals act as a barrier against evaporation from –80°C to 105°C. They are thin enough to pierce with a pipet tip for recovery of samples from individual wells, and they are suitable for use with automated systems, such as the ABI 3700 DNA analyzer. In addition to cold storage applications, they can be used for thermal cycling for sample volumes of 25 µl (96-well) or 5 µl (384-well).

Microseal 'A' FilmMicroseal 'A' film quickly and effectively seals the full range of Bio-Rad PCR plates and tubes. The pliant inner layer is designed to seal tightly during cycling yet to release smoothly to minimize the risk of aerosol formation and cross-contamination of samples. This film is easily cut for use with fewer than 96 wells; a peel-away release liner protects the sealing surface from contamination (not suitable for qPCR). An optional sealing roller provides a quick way to firmly seal Microseal 'A' film on an entire array of wells.

MSC-1001Microseal 'C' Optical Seals

PCR Plastic Consumables Microseal® Sealers

MSF-1001Microseal 'F' Foil




Plastics



Individual PCR Tubes with Attached Caps (0.2 ml)TFI-0201 PCR Tubes with Flat Caps (0.2 ml), clear, 1,000 TWI-0201 PCR Tubes with Domed Caps (0.2 ml), clear, 1,000

Individual PCR Tubes without Caps (0.2 ml)TBI-0201 PCR Tubes without Caps (0.2 ml), clear, 1,000

Individual PCR Tubes with Attached Caps (0.5 ml) TBI-0501 PCR Tubes with Flat Caps (0.5 ml), clear, 1,000 (2 bags of 500) TBI-0502 PCR Tubes with Flat Caps (0.5 ml), clear, 800 (8 bags of 100)

High-Profile Tube Strips without Caps (0.2 ml)TBS-0201 8-Tube Strips without Caps (0.2 ml), clear, 120 strips (960 PCR tubes) TBS-1201 12-Tube Strips without Caps (0.2 ml), clear, 100 strips (1,200 PCR tubes)

Low-Profile 8-Tube Strips without Caps (0.2 ml)TLS-0801 Low-Profile 8-Tube Strips without Caps (0.2 ml), clear, 120 (960 PCR tubes) TLS-0851 Low-Profile 8-Tube Strips without Caps (0.2 ml), white, 120 (960 PCR tubes)

Domed Cap StripsTCS-0801 Domed 8-Cap Strips, for 0.2 ml PCR tubes and plates, clear, 120 TCS-1201 Domed 12-Cap Strips, for 0.2 ml PCR tubes and plates, clear, 200

Optical Flat Cap StripsTCS-0803 Optical Flat 8-Cap Strips, for 0.2 ml PCR tubes and plates, ultraclear, 120

High-Profile Tube Strips with Domed Cap Strips (0.2 ml)TBC-0802 8-Tube Strips and Domed Cap Strips (0.2 ml), clear, 20 bags of 12 x 8-tube strips and 12 x 8-cap strips (1,920 PCR tubes and 1,920 caps)TBC-1202 12-Tube Strips and Domed Cap Strips (0.2 ml), clear, 20 bags of 8 x 12-tube strips and 8 x 12-cap strips (1,920 PCR tubes and 1,920 caps)

Capping Tools and RacksTRC-9601 PCR Tube Rack, ANSI/SBS standard, white, 10TRC-0501 96-Place Racks, with covers, for PCR tubes and unskirted and semi-skirted microplates, assorted colors, 5ECT-1000 Easy Cap Tool, ensures tight seal for 0.2 ml PCR tubes or 96-well microplatesECT-2000 Strip Cap Tool, for sealing 8- and 12-cap strips on PCR plates or tubes

Multiplate 48-Well PCR Plates MLP-4801 Multiplate 48-Well Unskirted PCR Plates, clear, 50 plates MLL-4801 Multiplate Low-Profile 48-Well Unskirted PCR Plates, clear, 50 plates MLL-4851 Multiplate Low-Profile 48-Well Unskirted PCR Plates, white, 50 plates

Multiplate 96-Well Unskirted PCR PlatesMLP-9601 Multiplate 96-Well Unskirted PCR Plates, clear, 25 plates MLP-9651 Multiplate 96-Well Unskirted PCR Plates, white, 25 plates MLP-9631 Multiplate 96-Well Unskirted PCR Plates, blue, 25 plates

Multiplate Low-Profile 96-Well Unskirted PCR PlatesMLL-9601 Multiplate Low-Profile 96-Well Unskirted PCR Plates, clear, 25 plates MLL-9651 Multiplate Low-Profile 96-Well Unskirted PCR Plates, white, 25 plates

iQ 96-Well PCR Plates223-9441 iQ 96-Well PCR Plates, 25 plates

PCR Plastic Consumables Ordering Information



Description Clear Wells White Wells Black Wells

Hard-Shell Low-Profile 96-Well Skirted PCR Plates White shell, 50 HSP-9601 HSP-9655 — Red shell, 50 HSP-9611 — — Yellow shell, 50 HSP-9621 — — Blue shell, 50 HSP-9631 HSP-9635 — Green shell, 50 HSP-9641 HSP-9645 — Black shell, 50 HSP-9661 HSP-9665 HSP-9666 White shell, bar-coded, 50 HSP-9901 HSP-9955 —

Hard-Shell High-Profile 96-Well Semi-Skirted PCR Plates Clear shell, 25 HSS-9601 — — Green shell, 25 HSS-9641 — — Black shell, 25 — HSS-9665 — Clear shell, bar-coded, 25 HSS-9901 — —

Hard-Shell 384-Well Standard PCR Plates Clear shell, 50 HSP-3801 HSP-3805 — White shell, 50 HSP-3851 — —Red shell, 50 HSP-3811 — — Yellow shell, 50 HSP-3821 — —Blue shell, 50 HSP-3831 — — Green shell, 50 HSP-3841 — — Black shell, 50 — HSP-3865 HSP-3866 Clear shell, bar-coded, 50 HSP-3901 HSP-3905 —

50 Plates 100 Plates, 100 Microseal 'C' Seals

Hard-Shell 384-Well 480 PCR Plates with Bar Code on Row A Side Clear shell, white well HSR-4805 HSR-4805K Clear shell, clear well HSR-4801 HSR-4801K


PCR Plate SealersMSA-5001 Microseal 'A' Film, package of 50 seals MSB-1001 Microseal 'B' Adhesive Seals, optically clear, 100 seals MSC-1001 Microseal 'C' Optical Seals, 100 seals MSF-1001 Microseal 'F' Foil, package of 100 seals MSR-0001 Sealing Roller, for film sealsADR-3296 Optical Compression Pad, for improved film sealing of 96-well plates in DNA Engine Opticon 2 and Chromo4 systemsADR-5001 Pressure Pad, uniformly distributes lid pressure for sealing film MSO-1001 Optical Film Sealing Kit, for 96-well plates, includes optical compression pad, 100 Microseal 'B' clear adhesive seals223-9444 Optical Sealing Tape, package of 100 sheets 223-9442 96-Well PCR Plate Sealing Mats, 5

PCR Plastic Consumables Ordering Information

Amplification Reagents and Plastics

Amplification: Consumables

Life ScienceGroup

11-1398 0911 Sig 0211Bulletin 6090 Rev B US/EG

Bio-Rad Laboratories, Inc.

Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 31 3689 6600 Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300 Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Malaysia 60 3 2117 5260 Mexico 52 555 488 7670 The Netherlands 0318 540666 New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3170 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 061 717 95 55 Taiwan 886 2 2578 7189 Thailand 66 2 6518311 United Kingdom 020 8328 2000

Cy is a trademark of GE Healthcare group companies. Eppendorf and Mastercycler are trademarks of Eppendorf AG. EvaGreen is a trademark of Biotium, Inc. Bio-Rad Laboratories, Inc. is licensed by Biotium, Inc. to sell reagents containing EvaGreen dye for use in real-time PCR, for research purposes only. FAM, ROX, StepOne, StepOnePlus, Veriti, and VIC are trademarks of Applera Corporation. HRM and Rotor-Gene are trademarks of QIAGEN GmbH. LightCycler is a trademark of Roche Diagnostics GmbH. LightScanner is a trademark of Idaho Technology Inc. Mx, Mx3000P, Mx3005P, and Mx4000 are trademarks of Stratagene Corporation. SYBR is a trademark of Molecular Probes, Inc. Bio-Rad Laboratories, Inc. is licensed by Molecular Probes, Inc. to sell reagents containing SYBR Green I for use in real-time PCR, for research purposes only. Texas Red is a trademark of Invitrogen Corporation. ViiA is a trademark of Life Technologies Corporation.

Notice regarding Bio-Rad thermal cyclers and real-time systems:Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser’s own internal research and development and for use in human in vitro diagnostics and all other applied fields under U.S. Patent Number 5,475,610 (Claims 1, 44, 158, 160–163, and 167 only), or corresponding claims in its non-U.S. counterpart, owned by Applera Corporation. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5' nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Bio-Rad’s real-time thermal cyclers are licensed real-time thermal cyclers under Applera’s U.S. Patent Number 6,814,934 B1 for use in research, human in vitro diagnostics, and all other fields except veterinary diagnostics.

Bio-Rad’s thermal cyclers and real-time thermal cyclers are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 6,767,512 and 7,074,367.

Practice of the patented 5' Nuclease Process requires a license from Applied Biosystems. The purchase of these products includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research when used with the separate purchase of Licensed Probe. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

Download - Bio-Rad Amplification Reagents

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