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CURRICULUM VITAE
NAMA : PARAMASARI DIRGAHAYU., dr., PhD
Lahir : Purwokerto, 21 April 1966
PENDIDIKAN
S1 : FK UNS Surakarta (1992)
S2-S3 : Tottori University Japan (1999-2003)
Post Doctoral : Tottori University Japan (2003-2004)
Field of Interest : Immuno-ParasitologyMolecular Biology and Biotechnology
PEKERJAAN:
Kepala Instalasi Parasitologi Klinik dan Mikologi RSDM (2007)
Kepala Pusat Bioteknologi dan Biodiversitas LPPM UNS (2005) Wa Ka Lab Parasitologi-Mikologi FK UNS (2007)
Koordinator International Office UNS (2009)
Sekretaris Lab Biomedik Terpadu FK UNS (2006)
Assesor BAN PT DIKTI (2007)
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Memahami peran ilmu Biologi molekuler di bidang
kedokteran dan kesehatan.
Mengerti dasar/ jargon ilmiah di bidang biologi molekuler
Memahami prinsip-prinsip dasar biologi molekuler dan
Bioteknologi dibidang kedokteran sehingga mampu
menyusun experimental design/memahami journal
penelitian internasional berbasis molekuler.
Pendahuluan
Kompetensi yang harus dicapai:
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SYL BUS M T KULI H BIOLOGI MOLEKULERKuliah Topik Pengampu
1 Filosofi Biologi Molekuler dan
Perannya di Bidang Kesehatan-1
(Prof . DR. dr. Ambar
Moedigdo., SpPA(K)
2 Filosofi Biologi Molekuler dan
Perannya di Bidang Kesehatan-1
(Prof . DR. dr. Ambar
Moedigdo., SpPA(K)
3 Struktur dan fungsi Sel, virus, DNA,
RNA dan Protein
(Afiono Agung S, dr., PhD)
4 Virologi Molekular dan aplikasi di bidangkedokteran Afiono Agung P.,dr.,PhD
5 Sel structure and Gene Expression Paramasari Dirgahayu
6 Tehnik Biologi Molekuler dan Aplikasinya di
bidang kedokteran-1
Diagnostik dan Terapi Molekuler
Paramasari Dirgahayu,Paramasari Dirgahayu, dr.,
PhD
7
8 Jurnal reading-1
Paramasari Dirgahayu, dr.,
PhD + (Afiono Agung S, dr.,
PhD)
9 Jurnal reading-2 (Paramasari Dirgahayu, dr.,
PhD + (Afiono Agung S, dr.,PhD)
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Fungsi Ilmu Bioteknologi
1. Diagnosa penyakit 2. Prevensi penyakit herediter
3. Terapi molekuler (stem cell terapi,
DNA terapi, dll)4. Riset/Klarifikasi host-Agent relation
5. Pembuatan vaksin
6. Forensik molekular
7. Epidemiologi molekuler
8. dll
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TEKNIK BIOLOGI MOLEKULER
(BIOTEKNOLOGI)
Digunakan dalam identifikasi ekspresi genetik/protein secarakuantitatif/kualitatif
Terpenting:
Preparasi sample : dari Invivo/In Vitro experiment
berupa DNA/RNA/Protein?
Tentukan metode identifikasi sample
1. Isolasi DNA
2. PCR
3. Western Blot
4. Northern Blot
5. Southern blot
6. Immunohistokimia
7. ELISA
8. Sequencing
9. Cloning
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Overview
BODY
ORGANS
CELLS
KROMOSOMS
DNA
GENE
PHENOTYPE
GENOTYPENUKLEUS
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What Is a Cell?
Cell Structurecomposition
(molecules)organization
Basic Unit of Life
metabolism
replication
cells acquire nutrients and convertthem to energy and self (growth)
all cells come from pre-existing cells
(reproduction)Laboratorium BIOMEDIK TERPADU Fakultas Kedokteran UNS
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Eukaryote vs Prokaryote
Eukaryotic cells have
membrane-bound
compartments with
specialized functions.
Mitochondria
Plasma
Membrane
Nucleus
Lysosome
ER
Golgi
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DNA
Deoxyribonucleic Acid
Double helix
Carries genetic information
Located in the nucleus
The monomer is a nucleotide
A phosphate
A ribose sugar A nitrogenous base
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What are the bases in DNA
A adenine
T thymine
C cytosine
G
guanine
Base pair rules
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Where is DNA located in the Cell?
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HUMAN CHROMOSOMES
(KARYOTYPE)
THE chromosmes visualizedas they originally spilled from
the lysed cells
THE chromosmes artifiallyline up in their numerical
order
CELLS
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Human Karyotype
22 pairs of autosomes
size bands
sex chromosomes females: X/X
males: X/Y diploid (2n)
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Chromosomes
genomic DNA is very longhumans, 6 x 109bp
~1.8 meters total length
total length reduced via
associates with proteins individual DNA molecules
packaged into chromosomes
each chromosome represents
one linear DNA molecule
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Where are the genes located?
Genes are located on
the chromosomes.
Every species has a
different number ofchromosomes.
There are two types of
chromosomes:
autosomes and sexchromosomes
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Promoter regionTATA boxCAAT box (in mammals)
GC box (GGGCGGG)
Initiation codon Stop codon
Polyadenylationsignal
AATAAExon 1 Exon 2 Exon 3 Exon 4
Intron 1 Intron 2 Intron 3
5 3
Eukaryote
Gene structure
Initiation codon Stop codon
Promoter region
Shine-Dalgarno box (AGGAGG)Pribnow box (TATAAT)-35 site (TTGACA) Prokaryote
5 3
ORF
Terminator
Terminator
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GENOME
App. 3200 KM (2000 MILES)
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Genes
locus = physical location on a chromosome
gene = unit of information (~protein)
}}
5'ATGAAATTTTTAATACATCAATTAAACAATGATTATATAAAAAATGATATA
AGATATAAATCAAATGAAGAGAAATATATGAGTAAAGTAGACTTCCTAGAAACA
ATGAAAAAAGATATATCAGAACTGTACGAAGAATATGGAACGAATAATGAATCGAACACTTCGTATAACATAACGATGGATTTTGTAAACAATTTTGATAGCAGAGAT
GGGTCATATATAACTGATATAAGCAGAGAAGAGTTGATGTTAAAACAACATAAT
ATAATGAGATTGCAAGATGAACAACTTGAATTTCTTGAAGGAACAACACAAAAT
TTAAAAAGTATTAGCTATAATATAAATAACGAAATACAAGTACATAATGAAATA
TTAGATGATATAGATAGAGATATGGATGAAACAAGTGATTTATTGGATAGAAAT
AGAAACATTTTTACCAGAATTACTAACAGTACGAGTAATTATTATTTGTACATG
TTAATTTGTTTATTAACAGTTACACTTTTCTTTTTGATCATAATACTTTAG-3'
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ekuens
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Cells Structure - animation
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Gene expression is theactivation of a gene that
results in a protein.
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GENE
EXPRESSION
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Summary
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Transcription 1
(making a mRNA copy of DNA)
The part of the DNA molecule (the gene) that the cell wants theinformation from to make a protein unwinds to expose the bases.
Free mRNA nucleotides in the nucleus base pair with one strand of
the unwound DNA molecule.
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Transcription 2
The mRNA copy is made with the help of RNA polymerase. This enzyme joins
up the mRNA nucleotides to make a mRNA strand.
This mRNA strand is a complementary copy of the DNA (gene)
The mRNA molecule leaves the nucleus via a nuclear pore into the cytoplasm
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tRNA
pick up their specific amino acids from
the cytoplasm
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Transcription- animation
T l ti
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Translation
RNA
Single stranded Does not contain thymine but
has uracil instead.
tRNA carries 3 base pair codefor specific amino acid.
Amino acids composepolypeptid chains.
One or more polypeptidechains compose a protein
proteins provide the
blueprints for ourcharacteristics andfunctions.
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In Prokaryotes there are three (3) regulatory elements thatcontrol gene expression.
1. Structural genes genes that code for a specificpolypeptide (protein).
2. Promoter DNA segment that recognizes RNA
polymerase.3. Operator element that serves as a binding site for an
inhibitor protein that blocks transcription.
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Eukaryote
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Translation - animation
A ribosome
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A ribosome
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Translation - outline
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Translation.
mRNA used to make polypeptide chain
(protein)
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1.
First the mRNA attaches itself to a ribosome (to the small subunit).
Six bases of the mRNA are exposed.A complementary tRNA molecule with its attached amino acid (methionine) base pairsvia its anticodon UAC with the AUG on the mRNA in the first position P.
Another tRNA base pairs with the other three mRNA bases in the ribosome at positionA.
The enzyme peptidyl transferase forms a peptide bond between the two amino acids.
The first tRNA (without its amino acid) leaves the ribosome.
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Translation 2
The ribosome moves along the mRNA to the next codon (three bases).
The second tRNA molecule moves into position P.
Another tRNA molecule pairs with the mRNA in position A bringing its amino acid.
A growing polypeptide is formed in this way until a stop codon is reached.
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End of Translation
A stop codon on the mRNA is reached and this signals the ribosome to leave the
mRNA. A newly synthesised protein is now complete!
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Translation continued
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Translation continued
4 A second tRNA molecule binds to theribosome
5 The amino acid from the first tRNA isconnected to the amino acid of thesecond tRNA with the assistance ofthe ribosome
6 This process continues until the stop
codon on the mRNA is reached
7 At the stop codon, a chemicalreaction frees the polypeptide chain
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Protein synthesis - animation
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HOW TO EXAMINEEXPRESSIONS OF THE GENES