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Beyond Laboratory Notebooks: Next Era Biological Data Hurdles forInformation Storage, Access and Distribution
Richard Slayden, PhD.Microbiology, Immunology & Pathology
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Evolution of the laboratory notebook: From recording to logging
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Example of data explosion: Traditional sequencing
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Published literature using AB SOLiDSOLiD sequencer: 14 days and 20 000 US$ (~10x coverage)
Proton: 4 hrs- 1,000’s bacteria, Human genome (~$2,000)
Example of data explosion: Next Generation Sequencing
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Workflow & complexity of the information required
Complexity of the data set: From the Bench to the Data
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Scientific Applications-Genome sequencing, whole transcriptome, modifications, structural variations
Workflow: Material type (ie. DNA or RNA) & sample preparation (Total RNA vs mRNA)
Workflow: library preparation & sequencing run-mate-pair or fragment
Computational Resources: Reference or de novo sequence assembly
Data reduction: Data Analysis- What portion of the data is analyzable, condensation, biologically relevant criteria
Secondary comparative analysis-Applied analysis, incorporation with historical data
Next Generation Sequencing: Complexity of the data set
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Current Data Storage:
Individual local computers or servers
Not readily accessible by multi local investigators
Not accessible by outside collaborators
Not routinely backed-up
Deletion of large raw data sets
Data cannot be integrated into multi-investigator programs
Current Data recording, management and distribution
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Beyond a single laboratory-Data access between experimental sites
Foothill Campus
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Beyond a single laboratory-interaction between experimental sites
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Beyond a single laboratory-interaction between experimental sites
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1. Data Storage-maintenance, cost, updating hardware, backup, secure, dynamic
2. Facilitate access to data files-from remote locations and software-software integration
3. Movement of data-without corruption more important than speed
4. Distribution of data files-across the US and beyond
5. Automated work processing-send data from remote location and analysis
6. Modern Help Desk-move beyond software updates and wireless mouse
7. Facilitate the development of the COLLABORATIVE LABORATORY NOTEBOOK
Towards a collaborative notebook-Envisioned Support Needs
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Questions: