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BD Stem Cell ResourceMarkers of Self-Renewal and Differentiation

23-9953-01

Ectoderm Markers Embryonic Stem Cells Mesoderm Markers Stem Cell Relevant SignalingAstrocytesA2B5CD44FGFR3

GFAPRan-2S100B

Embryonic Stem Cell MarkersAlkaline PhosphataseCD9CD15 (SSEA-1, Lewis X)CD24CD29 (Integrin β1)CD30CD49f (Integrin α6)CD133 (AC133, Prominin-1)CD194 (CCR4)CDw338 (ABCG2)c-MycCriptoDPPA4DPPA5 (ESG1)E-Cadherin (CD324)FGF-4FoxD3Frizzled-5GCNF (NR6A1)GCTM-2GDF-3Hsp27

Lefty-ALin-28Nanog (Human)Nanog (Mouse)Oct3/4PodocalyxinPRC2 (Suz12)Rex-1SFRP2Smad2/3Sox2SSEA-3SSEA-4STAT3STAT3 (pS727)STAT3 (pY705)STAT3-interacting protein 1TRA-1-60 AntigenTRA-1-81 AntigenUTF-1Zic3

Early Mesoderm MarkersBMP2BMP4BrachyuryCD31 (PECAM1)CD34 (Mucosialin, gp 105-120) Cryptic

MIXL1N-Cadherin (CD325)NF-YANodalSca-1 (Ly6A/E)Snail

Erk-MAPK Signalingbasic FGFC-RafERKERK1ERK1/2 (pT202/pY204)ERK2ERK3

Mek1Mek1 (pS298)Mek1/2 (pS222)Mek2p38 MAPK (PT180/pY182)Ras

TGF Beta SignalingBMPR-IICD119 (IFN-γ receptor α-chain)c-MycDP-1E2F-1E2F-2E2F-3Id1Id2

Id3p16 INK4p107p300PP2A Catalytic αROCK-IROCK-IISmad2/3SP1

Wnt Signaling14-3-3α-Cateninβ-Cateninδ-Cateninγ-CateninAktCaM Kinase IICasein Kinase I εCasein Kinase II αCasein Kinase II β

CBP (CREB-Binding Protein)GSK-3βGSK-3β (pY216)HDAC3Jagged1Neurogenin 3NicastrinNotch1PP2A Catalytic αWnt16

Jak-STAT SignalingCD130 (gp130)STAT1 (C-TERMINUS)STAT1 (pY701)STAT3STAT3 (pS727)STAT3 (pY705)

STAT4STAT4 (pY693)STAT5 (MGF)STAT5 (MGF) (PY694)STAT6STAT6 (pY641)

Notch SignalingCBP (CREB-Binding Protein)Jagged1Neurogenin 3

NicastrinNotch1

Heart - Cardiogenesis Markersα-ActininAnnexin VICardiac specific myosinCardiac Troponin ICardiac Troponin TCaveolin-2Caveolin-3CD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD66CD66cCD106 (VCAM-1)CD117 (SCF R, c-kit)CD144 (VE-cadherin)CD166 (ALCAM)

CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)Connexin-43DesminFlk-1 (KDR, VEGF-R2, Ly-73)GATA4GATA6HAND1HAND2M-CadherinMyogeninN-Cadherin (CD325)NF-YANkx2.5

Hemangioblast MarkersACEBracyuryCD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD133 (AC133, Prominin-1)CD144 (VE-cadherin)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)

E-Cadherin (CD324)EphB4Flk-1 (KDR, VEGF-R2, Ly-73)GATA1LMO-2PodocalyxinRunx1 (CBFA2)Tal (SCL)

Hematopoietic Stem Cell MarkersNegative Markers

CD2CD3 CD4CD10CD13CD14CD15 (SSEA-1, Lewis X)CD15s (Sialyl Lewis x)CD16CD19CD20CD24

CD33CD36CD38CD45 (Leukocyte Common

Antigen, Ly-5)CD45R (B220)CD48CD56 (NCAM)CD97CD114 (G-CSF Receptor)CD138 (Syndecan-1) GATA3

Mesenchymal Stem Cell Differentiation MarkersChondrocyte (Cartilage) Markers

AggrecanCD44CD151Collagen II

Matrillin-4OsteonectinSox-9

Mesenchymal Stem Cell Markers (Fat)Negative Markers

CD11b (Integrin αM chain)CD14CD19CD34 (Mucosialin, gp 105-120)

CD45 (Leukocyte Common Antigen, Ly-5)

CD79aCD117 (SCF R, c-kit)HLA-DR

Positive Markers

CD9CD10CD13CD29 (Integrin β1)CD44CD49d (Integrin α4)CD49e (Integrin α5)CD54 (ICAM-1)CD55CD59

CD73 (Ecto-5’-nucleotidase)CD90 (Thy-1)CD105 (Endoglin)CD106 (VCAM-1)CD146 (MCAM, MUC18)CD166 (ALCAM)FibronectinHLA IVimentin

Skeletal Muscle Markersα-ActininActinB-Enolase (ENO-3)CD146 (MCAM, MUC18)DesminM-CadherinMyf5

MyoDMyogeninMyosin heavy chainMyosin light chainN-Cadherin (CD325)TitinTroponin

Smooth Muscle MarkersCalmodulinDesmin

Smooth muscle myosinVimentin

Mesenchymal Stem Cell Markers (Bone Marrow)Negative Markers

CD11a (Integrin αL chain)CD11b (Integrin αM chain)CD14CD19CD31 (PECAM1)

CD34 (Mucosialin, gp 105-120) CD45 (Leukocyte Common

Antigen, Ly-5)CD79aHLA-DR

Positive Markers

CD10CD13CD14CD15 (SSEA-1, Lewis X)CD29 (Integrin β1)CD44H (Pgp-1, H-CAM)CD49d (Integrin α4)CD49e (Integrin α5)CD51 (Integrin αV)CD54 (ICAM-1)CD56 (NCAM)CD71 (Transferrin Receptor)CD73 (Ecto-5’-nucleotidase)CD90 (Thy-1)

CD105 (Endoglin)CD106 (VCAM-1)CD117 (SCF R, c-kit)CD120a (TNF Receptor Type I)CD120b (TNF Receptor Type II)CD166 (ALCAM)CD271 (p75, NGFR/NTR)Flk-1 (KDR, VEGF-R2, Ly-73)Ly-6A/E (Sca-1)NucleosteminSTRO-1TAZVimentin

Myoblast (Muscle) Markers

AcetylcholinesteraseActinB-Enolase (ENO-3)CD146 (MCAM, MUC18)DesminLactate Dehydrogenase (LDH)

Myf-5MyoDN-Cadherin (CD325)Pax3Pax7PK-M

Osteoblast (Bone) Markers

Alkaline PhosphataseCollagen 1Fibronectin

IGFBP-3OsteocalcinRUNX2

Positive Markers

Bmi-1CaM Kinase IVCD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD44CD59CD90 (Thy-1)CD90.1 (Thy-1.1)CD105 (Endoglin)CD106 (VCAM-1)CD117 (SCF R, c-kit)CD133 (AC133, Prominin-1)CD164CD184 (CXCR4)CD201 (EPCR)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)CDw93 (C1qRp)

CDw338 (ABCG2)DAZLFlk-1 (KDR, VEGF-R2, Ly-73)FOXO1G-CSF Gfi-1HOXB4Jak2Lmo2Ly-6A/E (Sca-1)MRP1N-Cadherin (CD325)NF-YANotch1P-glycoproteinRunx1 (CBFA2)Tal (SCL)WASP (Wiskott-Aldrich

Syndrome Protein)

Endothelial Cell MarkersCD14CD15 (SSEA-1, Lewis X)CD18 (Integrin β2 chain, CR3/CR4) CD29 (Integrin β1)CD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD36CD45 (Leukocyte Common

Antigen, Ly-5)CD45R (B220)CD49d (Integrin α4)CD49e (Integrin α5)CD49f (Integrin α6)CD54 (ICAM-1)CD56 (NCAM)CD62E (E-Selectin)CD62L (L-Selectin)CD62P (P-Selectin)CD102CD105 (Endoglin)CD106 (VCAM-1)CD109CD112CD116 (GM-CSF Receptor)CD117 (SCF R, c-kit)CD120a (TNF Receptor Type I)CD120b (TNF Receptor Type II)CD121a (IL-1 Receptor, Type I/p80)CD124 (IL-4 Receptor α)CD141 (Thrombomodulin)CD144 (VE-cadherin)CD146 (MCAM, MUC18)CD147 (Neurothelin)

CD151CD152 (CTLA-4)CD157CD166 (ALCAM)CD184 (CXCR4)CD192 (CCR2)CD201 (EPCR)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)CD209CD209a (CIRE, DC-SIGN)CD213a1CD213a2CD249CD252 (OX-40 Ligand)CD253 (TRAIL) CD262 (TRAIL-R2, DR5)CD322CDw93 (C1qRp)CDw145Flk-1 (KDR, VEGF-R2, Ly-73)HIF-1αIP-10Ly-6A/E (Sca-1)N-Cadherin (CD325)NestinSTAT3STAT3 (pS727)STAT3 (pY705)STAT3-interacting protein 1Vimentin

Endoderm MarkersDefinitive Endoderm Markersα-FetoproteinB-CateninCD184 (CXCR4)GATA4

HNF-1β (TCF-2)HNF-3β (FoxA2)N-Cadherin (CD325)SOX-17

Hepatic Endoderm Markersα-FetoproteinAlbuminCD29 (Integrin β1)CD44H (Pgp-1, H-CAM)CD49f (Integrin α6)

CD90 (Thy-1)HNF-1αHNF-1β (TCF-2)Tat-SF1

Pancreatic Endoderm MarkersCD49f (Integrin α6)Gad65GlucagonGlut2InsulinIslet-1Islet-2NeuroD1

Neurogenin 3Neuropilin-2Pancreatic polypeptidePax-6PDX-1SomatostatinSynaptophysin

Primitive Gut Tube MarkersCDX2 HNF-1β (TCF-2)

Induced Pluripotency Markersc-MycKLF4Lin28

NanogOct3/4Sox2

Primordial Germ Cell MarkersBlimp-1CD15 (SSEA-1, Lewis X)CD117 (SCF R, c-kit)DAZLFABP9Fragilis

NanosOct3/4Piwi/HiwiSteelStella (Dppa3)VASA (DDX4)

Trophoblast MarkersCDX2Cytokeratin 7

Trop-2

Early Ectoderm MarkersNestinNotch1

TP63

Neural Crest Cell MarkersCD49d (Integrin α4)CD57 (HNK-1)CD271 (p75, NGFR/NTR)

MASH1Neurogenin 3Notch1

Neural Stem Cell MarkersA2B5CD15 (SSEA-1, Lewis X)CD15s (Sialyl Lewis x)CD24CD29 (Integrin β1)CD49f (Integrin α6)CD54 (ICAM-1)CD81 (TAPA-1)CD95 (Fas/APO-1)CD133 (AC133, Prominin-1)CD146 (MCAM, MUC18)CD184 (CXCR4)CD271 (p75, NGFR/NTR)CD349 (Frizzled-9)

CDw338 (ABCG2)Ki-67MSI1 (Musashi-1)MSl2 (Musashi-2)Nanog (Mouse)NestinNogginNotch1Pax-6Prominin-2PSA-NCAMSox1Sox2Vimentin

Neuronal Markersα-Synucleinα-Synuclein (pY125)CD24CD56 (NCAM)CD81 (TAPA-1)CD90 (Thy-1)CD90.1 (Thy-1.1)CD90.2 (Thy-1.2)CD112CD271 (p75, NGFR/NTR)ChATContactinDoublecortinGABA B Receptor Gad65GAP-43 (Neuromodulin)GluR δ 2GluR2GluR5/6/7Glutamine SynthetaseJagged1MAP2 (a+b)MAP2BMASH1

mGluR1mGluR1αN-Cadherin (CD325)NestinNeurofilament NF-MNeuron-restricted progenitorsNeuropilin-2Nicastrinp150 GluedPax-5P-glycoproteinPSD-95Serotonin Receptor 5-HT 2ARSerotonin Receptor 5-HT 2BRSMNSynapsin ISynaptophysinSynaptotagminSyntaxinTauTrkBTubbyTyrosine HydroxylaseVimentin

Oligodendrocyte MarkersCD44CD44H (Pgp-1, H-CAM)CD140a (PDGFR α)Galactocerebroside CMBP

O1O4Olig1Olig2RIP

Skin Precursor Cell MarkersCRABP2FibronectinNestin

Sca-1 (Ly6A/E)Vimentin

White colored markers are available from BD Biosciences

bdbiosciences.com/stemcell

LIFR

Jak

Stat3C-myc Sox2 Oct4 Nanog Smads 2/3

Smads 175 MEK/ERK

p pp

-catenin

WntBMP4 bFGF

TGF- /Activin

LIF

JAK-STAT

Self Renewal

Wnt BMP-SMad Erk-MAPKTGF- /Activin

gp130

LiverSelf Renewing Pathway

Heart Skeletal Muscle Kidney Smooth Muscle

Endothelium

Hemangioblast

Proerythroblast

MegakaryocyteMyeloid Dendrtitic CellMonocyte

Monoblast Megakaryoblast

Skin and Hair

Glial Restricted Progenitor

Type 1 Astrocyte

Type 2 Astrocyte

Neuron

OligodendrocyteProgenitor

Oligodendrocyte

NeuronalRestrictedProgenitor

Neural CrestStem Cell

Neurons, GliaSmooth MuscleCardiac TissueChondrocytes

Osteocytes

MacrophageThrombocytes

(Platelets)

Myeloblast

Neutrophil BasophilEosinophil

NeutrophilicMyelocyte

BasophilicMyelocyte

EosinophilicMyelocyte

NeutrophilicBand Cell

BasophilicBand Cell

EosinophilicBand Cell

Erythroblast Progranulocyte

Normoblast

Reticulocyte

Erythrocyte(Red Blood Cell)

Egg

Primordial Germ Cell

Sperm

Osteoblast (Bone)

Adipocyte (Fat)Fibroblast

Myotube (Muscle) Plasmacytoid

Dendritic CellCommitted Lymphoid Progenitor

Pre B CellPre-NK Cell

NK Lymphoblast

NK Cell

B-Lymphoblast

B Cell

Plasma Cell

T Cell

T-Lymphoblast

Thymocyte

MyoblastChondrocyte (Cartilage)

Thymus Pancreas Thyroid Lung Intestine

Endoderm

Mesenchymal Stem Cell

Hematopoietic Stem Cell

Mesoderm

Neural Stem Cell Ectoderm Embryonic Stem Cell or Induced Pluripotent

Stem Cell