Isolation, screening and comprehensive characterization of candidate
microalgae for biofuel feedstock production and dairy effluent treatment: a
sustainable approach
Ashutosh Pandey1, Sameer Srivastava1, Sanjay Kumar2*
1Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad (U.P.), India
2School of Biochemical Engineering, Indian Institute of Technology (BHU), Varanasi (U.P.), India
*Corresponding author:
Dr Sanjay Kumar
Table S1
Wastewater sample collection sites for microalgae isolation.
Collection site Coordinates Date of Collection
Microalgal isolates
Pond_Near Mahabirpur Teliyarganj, Prayagraj (UP), India
25°30'12.0"N 81°52'02.7"E
December, 2015
ASK01-17
Aeration tank of dairy effluent plant Shyam Dairy products, Prayagraj (UP), India
25°38'26.0" N, 81°83'80.0" E
February 2016
ASK18-28
Table S2
Correlation equations of microalgae biomass (gL-1) vs absorbance (680 nm).
Microalga isolate Correlation equations
ASK01 Biomass (gL-1) = 0.2728 × Abs680 ̶ 0.0829 (R2 = 0.9717)
ASK03 Biomass (gL-1) = 0.2368 × Abs680 + 0.048 (R2 = 0.9790)
ASK07 Biomass (gL-1) = 0.2994 × Abs680 ̶ 0.0415 (R2 = 0.9838)
ASK08 Biomass (gL-1) = 0.3304 × Abs680 ̶ 0.0493 (R2 = 0.9987)
ASK09 Biomass (gL-1) = 0.2561 × Abs680 ̶ 0.0127 (R2 = 0.9797)
ASK10 Biomass (gL-1) = 0.3607 × Abs680 ̶ 0.0196 (R2 = 0.9711)
ASK14 Biomass (gL-1) = 0.4364 × Abs680 + 0.0028 (R2 = 0.9791)
ASK16 Biomass (gL-1) = 0.7184 × Abs680 ̶ 0.0497 (R2 = 0.9984)
ASK17 Biomass (gL-1) = 0.3634 × Abs680 ̶ 0.0145 (R2 = 0.9959)
ASK18 Biomass (gL-1) = 0.2533 × Abs680 +0.0543 (R2 = 0.9878)
ASK20 Biomass (gL-1) = 0.4173 × Abs680 ̶ 0.0365 (R2 = 0.9950)
ASK21 Biomass (gL-1) = 0.369 × Abs680 ̶ 0.0228 (R2 = 0.9740)
ASK22 Biomass (gL-1) = 0.4918 × Abs680 ̶ 0.0221 (R2 = 0.9986)
ASK25 Biomass (gL-1) = 0.4217 × Abs680 ̶ 0.0462 (R2 = 0.9864)
ASK27 Biomass (gL-1) = 0.4536 × Abs680 ̶ 0.0005 ( R2 = 0.9954)
Table S3
Summary list of isolated potential strains, source location, tentative taxonomic assignment
and 18S rRNA sequence similarity with BLAST taxa.
Strain IDSource
locationTaxonomic assignment Closest BLAST match with similarity
Sequence
size
ASK 01
25°30'12.0"N
81°52'02.7"E
Desmodesmus sp. (MF
939404)Desmodesmus armatus AB917135 (96%)
693
ASK 14 Chlorella sp. (MF 801313) Chlorella vulgaris KJ676110 (98%) 911
ASK 16 Scenedesmus sp. (MK209709) Scenedesmus sp CPC3 KF537681 (99%) 829
ASK 22 25°38'26.0"
N,
81°83'80.0" E
Scenedesmus sp. (MF 945631) Scenedesmus abundans X73995 (99%) 1709
ASK 25 Chlorella sp. (MF 801361) Chlorella sorokiniana KT852969 (99%) 823
ASK 27 Chlorella sp. (MF 927577) Chlorella sorokiniana KU948990 (99%) 821
Fig. S1 Microscopy of selected potential microalgae isolates (A) Desmodesmus sp. ASK01,
(B) Chlorella sp. ASK14, (C) Scenedesmus sp. ASK16, (D) Scenedesmus sp. ASK22, (E)
Chlorella sp. ASK25, (F) Chlorella sp. ASK27.
Fig. S2 FTIR spectrum of (A) dried biomass of ASK22, (B) lipid extracted biomass (LEMB)
of ASK22, (C) crude oil extracted from ASK22, (D) soybean oil and (E-F) TG and DTG of
the Scenedesmus sp. ASK22 biomass (MB) and lipid extracted biomass (LEMB) in the inert
(nitrogen) and oxidative (air) atmosphere.